Babak Mandoulakani - Academia.edu (original) (raw)

Papers by Babak Mandoulakani

پژوهشنامه اصلاح گیاهان زراعی, Aug 1, 2022

پژوهشنامه اصلاح گیاهان زراعی, Oct 1, 2020

HAL (Le Centre pour la Communication Scientifique Directe), 2015

Basal stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most important dis... more Basal stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most important diseases of sunflower. Quantitative trait loci (QTL) implicated in partial resistance to two isolates of S. sclerotiorum (SSU107 and SSKH41) were investigated using F 9 recombinant inbred lines (RILs) from the cross between sunflower parental lines PAC2 and RHA266. Experiments were conducted in completely randomized design with 3-6 replications under controlled conditions. The reaction of genotypes to basal stem rot disease was evaluated by measuring the percentage of necrosis area three days after inoculation. Combined analysis of experiments showed significant interactions between sunflower genotypes and S. sclerotiorum isolates suggesting that partial resistance to S. sclerotiorum should be isolate-specific in sunflower. QTLs were mapped using an updated high-density SSR and SNP linkage map. The map consisted of 210 SSRs and 11 genederived markers placed in 17 linkage groups (LGs). The total map length was 1,653.1 cM with a mean density of 1 marker per 7.44 cM. A total of 14 QTLs were detected for partial resistance to two isolates. The phenotypic variance explained by QTLs (R 2) ranged from 0.10 to 9.85. The sign of additive gene effects showed that favorable alleles for partial resistance to isolates came from both parents. Six QTLs were common between two isolates on LGs 1, 8 and 17, whereas the others were specific for each isolate. Colocalized QTLs on LG 1 were linked to the glutathione S-transferase gene (GST). The colocalized QTLs for partial resistance to basal stem rot isolates could be good candidates for marker assisted selection (MAS).

پژوهشنامه اصلاح گیاهان زراعی, Aug 1, 2022

Bulgarian Journal of Agricultural Science, Sep 26, 2017

inter-simple sequence repeat (issr) markers were used to assess genetic diversity and structure o... more inter-simple sequence repeat (issr) markers were used to assess genetic diversity and structure of two populations of Papaver bracteatum and one population of Papaver somniferum. Fifteen ISSR primers amplified 135 lociwhich 117 (86.6%) were polymorphic. The genetic diversity estimated by Shannon's information index (I) was 0.283 and 0.1 for P. bracteatum and P. somniferum, respectively. Eighty-three (61%) fragments were found to be useful as species-specific markers and 52 fragments were conserved between two species. Analysis of molecular variance (AMOVA) showed that the genetic variation mostly allocated to among species (64%). UPGMA clustering algorithm differentiated and classified studied genotypes and populations in three distinct groups. The result of the present study indicated a wide range of variation in the phenetic relationship among P. bracteatum and P. somniferum. Also, species-specific bands generated could be used to develop the species-specific sequence characterized amplified region (SCAR) markers.

Journal of Agricultural Science and Technology, Mar 1, 2018

Understanding of genetic diversity is essential in breeding programs and plant genetic resources ... more Understanding of genetic diversity is essential in breeding programs and plant genetic resources management. In this study, the genetic diversity of 77 individuals of Teucrium from different regions of Iran was investigated using 18 ISSR markers. A total number of 198 bands were detected by ISSR primers, of which 184 (92.9%) bands with an average of 10.2 bands per primer were polymorphic. The Percentage of Polymorphic bands (PPL) ranged from 80 (UBC834) to 100% (UBC811, 812, 818, 820, 825, 826, and UBC855). The average Polymorphic Information Content (PIC), Shannon's Information index (I), and Number of effective alleles (Ne) were 0.39, 0.526, and 1.6, respectively. The Analysis of Molecular Variance (AMOVA) revealed the higher level of genetic variation within populations (77%) compared to among populations (23%). Cluster analysis separated the individuals into three major groups using WPGMA based on Nei's genetic distance coefficients. In addition, a model-based Bayesian approach subdivided the individuals into three major subgroups. The results of this study revealed that estimation of population genetics parameters using ISSR markers can be applied for assessing the differences between Teucrium populations and management of the genetic resources.

Basil (Ocimum basillicum L.), one of the most important medicinal plants, contains monoterpene an... more Basil (Ocimum basillicum L.), one of the most important medicinal plants, contains monoterpene and sesquiterpene compounds. Linalool synthase (LIS) and Germacrene D synthase (GDS) are two key genes involved in terpenes biosynthesis. In the current investigation, cleaved amplified polymorphic sequence (CAPS) markers and sequencing were used to identify single nucleotide polymorphisms (SNPs) in both genes in five different basil populations. For this means, fragments of 600 and 583 bp from the coding sequences of these genes were amplified and digested by using Pst1 and Mse1 restriction enzymes. After retrieval of the sequences of the amplified gene fragments, SNPs were identified based on multiple sequence alignment in both genes in studied basil genotypes. Out of the nine SNPs identified in LIS gene, five occurred in exon region while the number of SNPs identified in intron was four. Of the total SNPs detected in this gene, the proportion of transition was 77.8%, with frequencies of A/G: 44.4% and T/C: 33.3% and C/G and C/A (transversion) with the same frequencies of 22.2%. In GDS gene, in total, 28 SNPs was detected (seven in exon and 21 in intron) of which 67.

Genetic diversity is necessary for plant breeders to obtain new cultivars either with high yield,... more Genetic diversity is necessary for plant breeders to obtain new cultivars either with high yield, better quality, more adapted to abiotic stress or more resistant to pest and pathogens. Retrotransposons are current component of plant genomes. Ubiquitous, activity and abundant of retrotransposons thorough the plant genome, make them useful molecular marker. We used inter-retrotransposon amplified polymorphism (IRAP) markers to assess genomic diversity levels and survey the activity of LTR retrotransposon elements in 10 confectionary sunflower populations. Out of 25 single and combined IRAP primers, 11 primers produced scorable and polymorphic banding patterns. A total of 116 loci were amplified by using 11 IRAP primers on confectionary populations. 110 loci out of 116 were polymorphic. The lowest (0.74) and highest (0.88) Nei genetic similarity was observed between Hamedan and Mashhad and Marand and Esfahan populations, respectively. Cluster analysis based on UPGMA algorithm grouped the studied populations in 3 main classes. Analysis of molecular variance revealed that the high part of total variation was due to within populations. So it will be better to do selection within populations in breeding programs. The results showed that retrotransposons are active in sunflower genome and they are inserted in sunflower genome as head-to-head, head-to-tail and tail-to-tail orientations. Our results showed that retrotransposon-based molecular markers can be used as a valuable tool for genomic assessment in sunflower.

Orobanche spp. is holoparasitic plant, parasitising roo... more Orobanche spp. is holoparasitic plant, parasitising roots of different crops. Genetic polymorphism was investigated among and within 3 Orobanche species collected from different regions of northwest Iran, using ISSR markers. Out of 34 ISSR primers tested, 20 were found to be polymorphic and produced clear bands .261 discernible bands were generated with 254 (94%) being polymorphic. Among studied species, "O. aegyptiaca" showed 2 unique bands. Clustering algorithm was divided collected Orobanche specimens into 6 main groups. It was obvious that genetic relationships among studied landraces did not have force tendency to associate with their geographic origins. According to AMOVA, 100% of the total variation was partitioned within species. Such variability is important for any attempt to develop resistant host crops against parasite.

Journal of Crop Breeding

A greenhouse factorial experiment was conducted in completely randomized design (CRD) with three ... more A greenhouse factorial experiment was conducted in completely randomized design (CRD) with three replications to investigate the effect of soil Zn deficiency stress on the expression of three genes involved in the synthesis of metal chelating agents (NAS1, NAS2 and NAS3) in bread wheat. Hamoun and Hirmand (Zn-efficient and inefficient cultivars, respectively) cultivars were planted under Zn deficiency (0 mg Zn / kg soil) and adequacy (5 mg Zn / kg soil) conditions and relative expression of the genes was measured in root and leaf of the cultivars at two growth stages: stem elongation (vegetative stage) and 30% of flowering (reproductive stage) using Real time PCR technique. The results revealed the highest expression of NAS1 (7-fold) and NAS2 (5fold) in Zn deficiency conditions in the root of Zn-efficient cultivar (Hamoun) at reproductive stage and in the leaf of the same cultivar at vegetative stage, respectively. The highest expression of NAS3 (7-fold) was observed in the root of Hamoun cultivar at reproductive stage. The expression of this gene was also enhanced in the leaf of both cultivars at reproductive stage under Zn deficiency conditions. In conclusion, the results of the present study demonstrated that the expression of NAS1, NAS2 and NAS3 genes in Zn-efficient cultivar under Zn deficiency stress was enhanced more than Zn-inefficient cultivar which probably facilitate Zn uptake and translocation in Zn-efficient cultivars through increasing the synthesis of Zn-chelating agents such as nicotianamine and mugineic acid in the root and leaf of the plants.

Journal of the Faculty of Agriculture, 2016

The study was conducted to determine the genetic diversity and correlation among the different tr... more The study was conducted to determine the genetic diversity and correlation among the different traits in tomato germplasm. Ninety-seven landraces collected from Igdir city (East Anatolian region) of Turkey and North-West of Iran, along with three commercial cultivars were studied during two years. Tomato genotypes showed genetic diversity in all the studied characters. Fruit shape of genotypes contained flattened, slightly flattened, rounded, cylindrical, cordate, ovate, obovate, pyriform and obcordate. In fruit color, red, dark red, orange, yellow and in fruit size, very small, small, intermediate and large were observed. Correlation analysis showed the genotypes with high yield, had large fruit, firm fruit, high blossom end rot and sun scald, small plant size, sparse foliage density, low seed amount/fruit size. Cluster analysis classified genotypes in six groups, so that genotypes with different vegetative and generative charactreristics were located separate groups. Keywords : Ge...

Journal of Agricultural Science and Technology, 2020

To extend the genetic base of Iranian tomato germplasm, 93 landraces were collected from the nort... more To extend the genetic base of Iranian tomato germplasm, 93 landraces were collected from the northwest of Iran and East Anatolian of Turkey, along with three commercial cultivars, and their genetic structure were studied using 39 SSR primers. Thirty-five polymorphic SSR loci generated a total of 118 alleles in the studied germplasm. Number of alleles per locus and effective number of alleles averaged 3.37 and 2.47, respectively. Expected heterozygosity of SSRs varied from 0.227 (TMS24) to 0.773 (LEta016), averaged 0.558. The mean number of alleles per genomic-SSRs (3.61) was more than that of ESTSSRs (2.66). Cluster analysis using Neighbour Joining (NJ) method placed 96 tomato genotypes in eight groups. Little congruence was found between NJ dendrogram and geographical distances. Genetic structure analysis of the germplasm using Bayesian method revealed two sub-populations and separated cherry tomatoes from the other landraces and commercial cultivars. Out of the 21 morphological ch...

Russian Journal of Plant Physiology, Jul 1, 2021

A greenhouse experiment was conducted to investigate the expression levels of six ZRT/IRT like-pr... more A greenhouse experiment was conducted to investigate the expression levels of six ZRT/IRT like-protein (ZIP) genes in roots and leaves of bread wheat cultivars (Triticum aestivum L.) under soil Zn deficiency conditions. ‘Bayat’ and ‘Niknejad’ (Zn-efficient) and ‘Hirmand’ and ‘Karaj1’ (Zn-inefficient) cultivars were grown in soil Zn deficiency and control conditions with sufficient level of zinc in the medium and genes expression levels were studied in roots and leaves of the cultivars at 28 days after germination (vegetative) and 30% of heading (reproductive). The leaf, root and grain Zn concentration and content were also measured. Analysis of gene expression data revealed that in Zn starvation conditions, the expression of some ZIP genes in Zn-efficient cultivars was significantly (P ≤ 0.01) higher than those of Zn-inefficient cultivars. ZIP9 expression in root was significantly (P ≤ 0.01) higher than that of leaf, while the expression of ZIP7, ZIP10, ZIP14, and ZIP16 in root was significantly (P ≤ 0.01) lower than those of leaf. The relative expression of ZIP9, ZIP10, ZIP11, ZIP14 and ZIP16 at reproductive stage was significantly (P ≤ 0.01) higher than those in the vegetative stage. A positive and significant (P ≤ 0.01) correlation was observed between the expression of ZIP9, ZIP10 and ZIP14 in leaf at vegetative stage and both root and leaf Zn contents at the same stage under Zn deficiency conditions. The expression of ZIP7 in leaf at reproductive stage was also strongly associated with the grain Zn content under Zn deficiency conditions, hence ZIP7 could be proposed for the biofortification of bread wheat cultivars.

Seed and Plant Improvment Journal, 2008

Yr15 تسا هب هك 31 يم ناشن تمواقم درز گنز داژن دهد . موزومورك يور نژ نيا 1BS ناكم تسا هدش يباي . ه... more Yr15 تسا هب هك 31 يم ناشن تمواقم درز گنز داژن دهد . موزومورك يور نژ نيا 1BS ناكم تسا هدش يباي . هناسمه تهج مدق نيلوا ناونع هب ناكم ،هشقن ساسارب نژ نيا يزاس تيعمج كي زا هدافتسا اب نآ قيقد يباي F2 لماش 825 دش ماجنا درف . نوزوپسنرتورتر روضح هب هجوت اب وركوي يحاون رد اه نژ فارطا و نيتام زا ًاتدمع ،اه نوزوپسنرتورتر رب ينتبم ياهرگناشن دش هدافتسا اه . رگناشن هس SSR رگناشن هس ، IRAP رگناشن تفه و REMAP اب زا رتمك هلصاف 2 يتناس دش عابشا نژ فارطا يكيتنژ هشقن و يياسانش نژ اب ناگروم . تمس رد اهرگناشن يخرب اد نامزمه قرفت نژ يرمورتناس دنتش . ياهرگناشن زا يكي IRAP رگناشن ود و REMAP يصاصتخا ياهرگناشن هب و رگناشن هك دندش ليدبت زرابمه زرابمه و يصاصتخا SC792 تشاد نامزمه قرفت نژ اب . ياهرگناشن و يصاصتخا زرابمه SC1600 و SC1028 هلصاف رد ار نژ يتناس كي يم هطاحا ناگروم دندرك . يم اهرگناشن نيا اوت يارب دنن دنوش هدافتسا نژ نيا هشقن ساسارب يزاس هناسمه و رگناشن كمك هب شنيزگ . هك داد ناشن قيقحت نيا جياتن ناكم يارب ينوزوپسنرتورتر ياهرگناشن نژ قيقد يباي يم يعارز مهم تافص هدننك لرتنك ياه ديفم رايسب دنناوت دنشاب . ا...

Journal of Agricultural Science and Technology, 2020

Retrotransposons (RTNs) are a major source of genomic changes in plant genomes and, therefore, ar... more Retrotransposons (RTNs) are a major source of genomic changes in plant genomes and, therefore, are extensively used as ideal molecular markers for genetic variability, DNA fingerprinting, and genetic mapping studies in plant species. In the present study, two RTNbased marker systems, inter-retrotransposon amplified polymorphisms (IRAPs), and the retrotransposon-microsatellite amplified polymorphisms (REMAPs) were used to assess genetic variability and structure in a collection of 94 durum wheat genotypes. In general, 63 and 141 loci were amplified using 6 IRAP and 15 REMAP primers, respectively. Percentage of polymorphic loci (PPL) in the studied collection for IRAP and REMAP markers were 47.15% and 47.81%, respectively. The average of expected heterozygosity (He), number of effective alleles (Ne), and Shannon's information index (I), separately estimated based on IRAP and REMAP data, were not considerably different. A model-based Bayesian method and cluster analysis using Neigh...

Journal of Crop Breeding, Oct 10, 2021

پژوهشنامه اصلاح گیاهان زراعی, Aug 1, 2022

پژوهشنامه اصلاح گیاهان زراعی, Oct 1, 2020

HAL (Le Centre pour la Communication Scientifique Directe), 2015

پژوهشنامه اصلاح گیاهان زراعی, Aug 1, 2022

Bulgarian Journal of Agricultural Science, Sep 26, 2017

Journal of Agricultural Science and Technology, Mar 1, 2018

Journal of Crop Breeding

Journal of the Faculty of Agriculture, 2016

Journal of Agricultural Science and Technology, 2020

Russian Journal of Plant Physiology, Jul 1, 2021

Seed and Plant Improvment Journal, 2008

Journal of Agricultural Science and Technology, 2020

Journal of Crop Breeding, Oct 10, 2021