Barbara Stachowiak - Academia.edu (original) (raw)

Papers by Barbara Stachowiak

Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality

Piwo jest popularnym i najbardziej preferowanym napojem alkoholowym wśród konsumentów na świecie.... more Piwo jest popularnym i najbardziej preferowanym napojem alkoholowym wśród konsumentów na świecie. W Polsce jego roczne spożycie w przeliczeniu na 1 mieszkańca wynosi blisko 100 litrów, dzięki czemu nasz kraj znajduje się w gronie światowych liderów w tym zakresie. Na przestrzeni ostatnich dwudziestu lat postrzeganie piwa uległo znacznemu przeorientowaniu. Rewolucja piwna i związane z nią zmiany w branży browarniczej przyczyniły się do uzyskania przez ten trunek prestiżowej pozycji. Bardzo dynamicznie rozwija się rynek innowacyjnych piw rzemieślniczych, które stanowią ciekawą, a zarazem smakowo i jakościowo zróżnicowaną alternatywę dla marek masowych. Wśród nich dominują „piwne specjalności”. Mianem tym określa się zwykle wszystkie piwa fermentacji górnej oraz dolnej, innej niż tradycyjne lagery i pilsy. Pojawił się trend tzw. premiumizacji, wciąż wzrastają oczekiwania konsumentów wobec jakości piwa dotyczące jego składu, właściwości sensorycznych oraz potencjału prozdrowotnego. Na r...

Applied Sciences

Predicting yield at an early growth stage allows one to optimize the amount and timing of nitroge... more Predicting yield at an early growth stage allows one to optimize the amount and timing of nitrogen fertilization to maximize crop yield. The aim of the study was to determine how different nitrogen doses would affect the nutrition of two maize varieties (Zea mays L.) with this element and their yields, and to demonstrate the potential of using the SPAD index as an early-stage predictor of dry matter yield (DMY) of maize biomass. The field experiment, with different pre- and post-sown N fertilizer doses, was conducted for two years in Poland. The two-factor experiment was set up in a randomized block design with three field replicates. There were four mineral fertilization variants: (I) 120 kg N·ha−1 without P and K fertilization, (II) 120 kg N·ha−1 + PK, (III) 160 kg N·ha−1 + PK, and (IV) 200 kg N·ha−1 + PK. The second-order factor was two stay-green maize varieties: Delitop (FAO 240) and Magitop (FAO 270). The mean values of the SPAD index of maize depended on the nitrogen dose. Wh...

Sustainability

Failure to adjust the fertilization system to quantitative needs, and especially to the dynamics ... more Failure to adjust the fertilization system to quantitative needs, and especially to the dynamics of mineral demand, causes plant metabolism disorders, low mineral utilization by the plant, and an increased risk of environmental pollution. Additionally, unbalanced mineral fertilization may reduce the assimilation surface actively involved in photosynthesis, which determines the yield potential of individual varieties. The aim of the strict field experiment was to determine the responses of two types of maize varieties (Zea mays L.) to treatments with different nutrient management systems, as expressed by the growth analysis of active organs during photosynthesis, SPAD (soil and plant analysis development) leaf greenness index, green mass yield, and unit nitrogen productivity from PFPFN mineral fertilization (partial factor productivity fertilizer nitrogen). It was demonstrated that the total area of leaf blades of a single plant and the LAI (leaf area index) value were significantly ...

Plants

The pine (Pinus L.) is the largest and most heteromorphic plant genus of the pine family (Pinacea... more The pine (Pinus L.) is the largest and most heteromorphic plant genus of the pine family (Pinaceae Lindl.), which grows almost exclusively in the northern hemisphere. The demand for plant-based remedies, supplements and functional food is growing worldwide. Although pine-based products are widely available in many parts of the world, they are almost absent as food ingredients. The literature shows the beneficial effects of pine preparations on human health. Despite the wide geographical distribution of pine trees in the natural environment, there are very few data in the literature on the widespread use of pine in food technology. This study aims to present, characterise and evaluate the content of phytochemicals in pine trees, including shoots, bark and conifer needles, as well as to summarise the available data on their health-promoting and functional properties, and the potential of their use in food and the pharmaceutical industry to support health. Various species of pine tree ...

Agronomy

Field experiments were carried out at the Department of Agronomy of the Poznań University of Life... more Field experiments were carried out at the Department of Agronomy of the Poznań University of Life Sciences to determine the effect of the depth of NP fertilization placement in maize cultivation on the number of plants after emergence. The adopted assumptions were verified based on a six-year field experiment involving four depths of NP fertilizer application (A1—0 cm (broadcast), A2—5 cm (in rows), A3—10 cm (in rows), A4—15 cm (in rows)). The objective of this study was to assess NP fertilizer placement depth, in conjunction with the year, on the number of maize (Zea mays L.) plants after emergence using the additive main effects and multiplicative interaction model. The number of plants after emergence decreased with the depth of NP fertilization in the soil profile, confirming the high dependence of maize on phosphorus and nitrogen availability, as well as greater subsoil loosening during placement. The number of plants after emergence for the experimental NP fertilizer placement...

Molecules

Xanthophyll astaxanthin, which is commonly used in aquaculture, is one of the most expensive and ... more Xanthophyll astaxanthin, which is commonly used in aquaculture, is one of the most expensive and important industrial pigments. It is responsible for the pink and red color of salmonid meat and shrimp. Due to having the strongest anti-oxidative properties among carotenoids and other health benefits, natural astaxanthin is used in nutraceuticals and cosmetics, and in some countries, occasionally, to fortify foods and beverages. Its use in food technology is limited due to the unknown effects of long-term consumption of synthetic astaxanthin on human health as well as few sources and the high cost of natural astaxanthin. The article characterizes the structure, health-promoting properties, commercial sources and industrial use of astaxanthin. It presents the possibilities and limitations of the use of astaxanthin in food technology, considering its costs and food safety. It also presents the possibilities of stabilizing astaxanthin and improving its bioavailability by means of micro- ...

Molecules, 2021

►► APA citation: Siejak, P., Smułek, W., Fathordobady, F., Grygier, A., Baranowska, H.M., Rudzińs... more ►► APA citation: Siejak, P., Smułek, W., Fathordobady, F., Grygier, A., Baranowska, H.M., Rudzińska, M., Masewicz, Ł., Jarzębska, M., Nowakowski, P.T., Makiej, A., Kazemian, P., Drobnik, P., Stachowiak, B., Jarzębski, M., & Pratap-Singh, A. (2021). Multidisciplinary studies of folk medicine “Five Thieves’ Oil” (Olejek Pięciu Złodziei) components. Molecules, 26(10), 2931. ◄◄ To meet the growing interest in natural antibacterial agents, we evaluated the physicochemical and biological properties of the folk medicine known as “five thieves’ oil” (Polish name: olejek pięciu złodziei). Five thieves’ oil consists of a mixture of five oils: rosemary, lemon, clove, eucalyptus, and cinnamon. In this study, we performed gas chromatography, FTIR, and UV–vis spectroscopic analysis, as well as L-a-b color tests, contact angle determination, and surface tension determination. To verify its antibacterial activity, the metabolic activity and changes in cell membrane permeability of bacteria of the g...

FEMS Microbiology Letters

The oleaginous yeast Rhodosporidium diobovatum is a poorly described producer of carotenoids and ... more The oleaginous yeast Rhodosporidium diobovatum is a poorly described producer of carotenoids and may be of interest in biotechnology. This study investigated the effects of culture medium and illumination on effective carotenoid production by R. diobovatum IMB Y-5023. Yeast was cultured on carrot, bran extract, and modified yeast malt (YM) medium at illuminations in the 0-5000 lx range. Biomass, total carotenoids and their profile were assessed after cultivation. In order to compare samples, cluster analysis and principal component analysis were used to visualize the relationships between the variables and samples. Results show that it is not illumination but culture medium that is the main factor determining the quantity and proportions of carotenoids produced by R. diobovatum IMB Y-5023. The yeast grew and produced pigments throughout the tested range of illumination intensity. The modified YM medium was optimal for carotenogenesis. In cultures on that medium, the highest carotenoid yields of 24.98 mg g −1 dry cell weight and 77 mg L −1 were recorded. It was found that this yeast is capable of assimilating oligosaccharides and can grow and produce carotenoids in low-glucose media containing DP3 and DP4. Moreover R. diobovatum IMB Y-5023 produced lycopene as the main pigment independently of the culture conditions.

International Journal of Dairy Technology

Carbohydrate Polymers, 2016

Some ß-glucans can be easily extracted from Basidiomycete mushrooms but commonly used extraction ... more Some ß-glucans can be easily extracted from Basidiomycete mushrooms but commonly used extraction procedures are not satisfactory. A simultaneous method for acid extraction and deproteinization in the case of Pleurotus ostreatus was developed using response surface methodology. The optimized extraction conditions proposed here (30°C, 3.8% HCl, 300min, stirring) allow for the simultaneous extraction and deproteinization of polysaccharides. Additionally, the acid extraction yield was 7 times greater than that of hot water extraction. The combined enzymatic digestion with lyticase, ß-glucanase, exo-1,3-ß-d-glucanase, and ß-glucosidase results elucidated that an extract containing ß-1,3-ß-1,6-ß-1,4-glucan. The gel permeation chromatography (GPC) results showed that the two glucan fractions obtained do not contain linked proteins. The weight average molecular weight of the first fraction (Mw=1137kDa) was 60 times higher than that of the second fraction (Mw=19kDa).

Zywnosc Nauka Technologia Jakosc, 2006

Zywnosc: Nauka, Technologia, Jakosc

The study investigated the effect of a Bacillus globisporus strain and produced super-natants on ... more The study investigated the effect of a Bacillus globisporus strain and produced super-natants on growth of selected plant pathogens, i.e. Alternaria alternata, Botrytis cinerea, Clado-sporium spp., Trichothecium roseum, Fusarium oxysporum, F. solani, Trichoderma viride, Rhi-zoctonia solani, Sclerotinia sclerotiorum, Verticillium dahliae. The highest fungistatic activity of bacterial cultures was found against R. solani, S. sclerotiorum, B. cinerea and T. roseum, whereas in case of the supernatant, it was found against R. solani and S. sclerotiorum. At identical concentrations , the fungistatic activity of B. globisporus culture appeared stronger than that of the super-natant. No significant effect of bacterial culture concentration in the medium was observed. Growth of Cladosporium spp., V. dahliae, T. viride, and A. alternata was found both after seven and 10 days of the experiment. In case of the supernatant, this situation was observed only for F. oxysporum, after seven days.

Zywnosc: Nauka, Technologia, Jakosc

S t r e s z c z e n i e Astaksantyna naley do grupy barwników karotenoidowych. Znajduje ona powsz... more S t r e s z c z e n i e Astaksantyna naley do grupy barwników karotenoidowych. Znajduje ona powszechne zastosowanie jako niezbdny składnik pasz w przemysłowej hodowli łososi, pstrgów i krewetek, nadajc tkankom tych zwierzt podane przez konsumenta, charakterystyczne róowoczerwone zabarwienie. Ponadto, astaksantyna charakteryzuje si du aktywnoci przeciwutleniajc, najwysz wród znanych karotenoidów i 100-500-krotnie wysz w porównaniu z α-tokoferolem. Wród preparatów astaksantyny, wystpujcych na wiatowym rynku, 95% zawiera barwnik syntetyczny-mniej stabilny od astaksantyny pozyskiwanej ze ródeł naturalnych. Ograniczony zakres stosowania astaksantyny naturalnej wynika z kosztów jej otrzymywania, głównie na drodze syntezy mikrobiologicznej z udziałem alg Haematococcus pluvialis. Potencjalnym ródłem astaksantyny naturalnej s drode Phaffia rhodozyma. Jako ewentualne przemysłowe ródło tego barwnika wykazuj one wiele zalet. Przede wszystkim astaksantyna jest głównym produkowanym przez te drode...

The effect of pre-culturing of Pleurotus ostreatus in wheat bran extract after consequent heat tr... more The effect of pre-culturing of Pleurotus ostreatus in wheat bran extract after consequent heat treatment (5 min 100°C) and without it on the growth rate of the yeast Rhodosporidium diobovatum and Xanthophyllomyces dendrorhous was investigated. It was found that the pre-cultivation of Pleurotus ostreatus from 2 to 12 days thereafter depressed the yeast growth rate as compared to the base culture medium. In that case, if Pleurotus ostreatus was precultured in the base medium, and then treated by heat, Rhodosporidium diobovatum and Xanthophyllomyces dendrorhous growth rate increased by 60-80% as compared with the base medium cultivation. It is shown that the composition of the base medium after culturing of Pleurotus ostreatus and consequent heat treatment changed greatly, in particular glucose content increased. It has been shown that the presence of lipid components in natural nutrient media inhibits yeast growth rate.

Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists, 2013

Astaxanthin is a xanthophill pigment with commercial application in the aquaculture, pharmaceutic... more Astaxanthin is a xanthophill pigment with commercial application in the aquaculture, pharmaceutical, food and cosmetic industries. The red yeast Xanthophyllomyces dendrorhous is one of the most promising microorganisms for its industrial production. However, astaxanthin content in wild yeast strains is low. Pigment production by X. dendrorhous can be improved by mutagenesis. The aim of the study was to assess the efficiency of four mutagens: UV radiation, benomyl, ethyl methanesulfonate and ethidium bromide in generating asthaxanthin-hyperproducing strains of the yeast Xanthophyllomyces dendrorhous DSM 5626. Mutations with benomyl, ethidium bromide and UV radiation generated a group of hyperpigmented mutants exhibiting increases up to 100% in astaxanthin content. Ethyl methanesulfonate turned out to be useless in this respect.

Indian Journal of Microbiology, 2012

The study evaluated the effect of media based on plant extracts: potato, carrot and barley malt b... more The study evaluated the effect of media based on plant extracts: potato, carrot and barley malt broth, on growth and astaxanthin synthesis by yeast Xanthophyllomyces dendrorhous DSM 5626 and its mutants. The carrot medium promoted carotenogenesis most effectively. In cultures on this medium the highest volumetric and cellular concentrations of astaxanthin were recorded for four out of five tested strains. Also the share of astaxanthin in the total carotenoids produced by the tested strains was the highest.

Food Science and Biotechnology, 2013

The aim of the study was to select optimal illumination for Xanthophyllomyces dendrorhous DSM 562... more The aim of the study was to select optimal illumination for Xanthophyllomyces dendrorhous DSM 5626 and its mutants: 26UV, 10BE, 13B, and 34B for astaxanthin production. The strains were cultivated on YM medium at an illumination of 0-5,000 lx. The highest yields of astaxanthin were found in cultures at 600 lx. The maximum pigment production for the parental strain was 0.95 mg/L and 0.19 g/kg dry cell weight (DCW), while for mutants 1.23-1.51 mg/L and 0.34-0.39 g/kg DCW. It was calculated that the application of adequate values of illumination (within the range of 670-718 lx), determined on the basis of regression models, may improve astaxanthin production by approximately 5-15%, depending on the strain. This was confirmed in the verification experiments for the parental strain and mutants: 10BE, 13B, 26UV, but for 34B at 718 lx the volumetric astaxanthin production was found to be higher than predicted by the model.

... Antioxidant potential of carotenoid extracts from Phaffia rhodozyma mutants in linoleic acid ... more ... Antioxidant potential of carotenoid extracts from Phaffia rhodozyma mutants in linoleic acid emulsion Barbara Stachowiak, Anna Gramza-Michałowska, Jacek Nowak, Julita Reguła Wydział Nauk o Żywności i Żywieniu, Uniwersytet Przyrodniczy w Poznaniu Introduction. ...

Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality

Piwo jest popularnym i najbardziej preferowanym napojem alkoholowym wśród konsumentów na świecie.... more Piwo jest popularnym i najbardziej preferowanym napojem alkoholowym wśród konsumentów na świecie. W Polsce jego roczne spożycie w przeliczeniu na 1 mieszkańca wynosi blisko 100 litrów, dzięki czemu nasz kraj znajduje się w gronie światowych liderów w tym zakresie. Na przestrzeni ostatnich dwudziestu lat postrzeganie piwa uległo znacznemu przeorientowaniu. Rewolucja piwna i związane z nią zmiany w branży browarniczej przyczyniły się do uzyskania przez ten trunek prestiżowej pozycji. Bardzo dynamicznie rozwija się rynek innowacyjnych piw rzemieślniczych, które stanowią ciekawą, a zarazem smakowo i jakościowo zróżnicowaną alternatywę dla marek masowych. Wśród nich dominują „piwne specjalności”. Mianem tym określa się zwykle wszystkie piwa fermentacji górnej oraz dolnej, innej niż tradycyjne lagery i pilsy. Pojawił się trend tzw. premiumizacji, wciąż wzrastają oczekiwania konsumentów wobec jakości piwa dotyczące jego składu, właściwości sensorycznych oraz potencjału prozdrowotnego. Na r...

Applied Sciences

Predicting yield at an early growth stage allows one to optimize the amount and timing of nitroge... more Predicting yield at an early growth stage allows one to optimize the amount and timing of nitrogen fertilization to maximize crop yield. The aim of the study was to determine how different nitrogen doses would affect the nutrition of two maize varieties (Zea mays L.) with this element and their yields, and to demonstrate the potential of using the SPAD index as an early-stage predictor of dry matter yield (DMY) of maize biomass. The field experiment, with different pre- and post-sown N fertilizer doses, was conducted for two years in Poland. The two-factor experiment was set up in a randomized block design with three field replicates. There were four mineral fertilization variants: (I) 120 kg N·ha−1 without P and K fertilization, (II) 120 kg N·ha−1 + PK, (III) 160 kg N·ha−1 + PK, and (IV) 200 kg N·ha−1 + PK. The second-order factor was two stay-green maize varieties: Delitop (FAO 240) and Magitop (FAO 270). The mean values of the SPAD index of maize depended on the nitrogen dose. Wh...

Sustainability

Failure to adjust the fertilization system to quantitative needs, and especially to the dynamics ... more Failure to adjust the fertilization system to quantitative needs, and especially to the dynamics of mineral demand, causes plant metabolism disorders, low mineral utilization by the plant, and an increased risk of environmental pollution. Additionally, unbalanced mineral fertilization may reduce the assimilation surface actively involved in photosynthesis, which determines the yield potential of individual varieties. The aim of the strict field experiment was to determine the responses of two types of maize varieties (Zea mays L.) to treatments with different nutrient management systems, as expressed by the growth analysis of active organs during photosynthesis, SPAD (soil and plant analysis development) leaf greenness index, green mass yield, and unit nitrogen productivity from PFPFN mineral fertilization (partial factor productivity fertilizer nitrogen). It was demonstrated that the total area of leaf blades of a single plant and the LAI (leaf area index) value were significantly ...

Plants

The pine (Pinus L.) is the largest and most heteromorphic plant genus of the pine family (Pinacea... more The pine (Pinus L.) is the largest and most heteromorphic plant genus of the pine family (Pinaceae Lindl.), which grows almost exclusively in the northern hemisphere. The demand for plant-based remedies, supplements and functional food is growing worldwide. Although pine-based products are widely available in many parts of the world, they are almost absent as food ingredients. The literature shows the beneficial effects of pine preparations on human health. Despite the wide geographical distribution of pine trees in the natural environment, there are very few data in the literature on the widespread use of pine in food technology. This study aims to present, characterise and evaluate the content of phytochemicals in pine trees, including shoots, bark and conifer needles, as well as to summarise the available data on their health-promoting and functional properties, and the potential of their use in food and the pharmaceutical industry to support health. Various species of pine tree ...

Agronomy

Field experiments were carried out at the Department of Agronomy of the Poznań University of Life... more Field experiments were carried out at the Department of Agronomy of the Poznań University of Life Sciences to determine the effect of the depth of NP fertilization placement in maize cultivation on the number of plants after emergence. The adopted assumptions were verified based on a six-year field experiment involving four depths of NP fertilizer application (A1—0 cm (broadcast), A2—5 cm (in rows), A3—10 cm (in rows), A4—15 cm (in rows)). The objective of this study was to assess NP fertilizer placement depth, in conjunction with the year, on the number of maize (Zea mays L.) plants after emergence using the additive main effects and multiplicative interaction model. The number of plants after emergence decreased with the depth of NP fertilization in the soil profile, confirming the high dependence of maize on phosphorus and nitrogen availability, as well as greater subsoil loosening during placement. The number of plants after emergence for the experimental NP fertilizer placement...

Molecules

Xanthophyll astaxanthin, which is commonly used in aquaculture, is one of the most expensive and ... more Xanthophyll astaxanthin, which is commonly used in aquaculture, is one of the most expensive and important industrial pigments. It is responsible for the pink and red color of salmonid meat and shrimp. Due to having the strongest anti-oxidative properties among carotenoids and other health benefits, natural astaxanthin is used in nutraceuticals and cosmetics, and in some countries, occasionally, to fortify foods and beverages. Its use in food technology is limited due to the unknown effects of long-term consumption of synthetic astaxanthin on human health as well as few sources and the high cost of natural astaxanthin. The article characterizes the structure, health-promoting properties, commercial sources and industrial use of astaxanthin. It presents the possibilities and limitations of the use of astaxanthin in food technology, considering its costs and food safety. It also presents the possibilities of stabilizing astaxanthin and improving its bioavailability by means of micro- ...

Molecules, 2021

►► APA citation: Siejak, P., Smułek, W., Fathordobady, F., Grygier, A., Baranowska, H.M., Rudzińs... more ►► APA citation: Siejak, P., Smułek, W., Fathordobady, F., Grygier, A., Baranowska, H.M., Rudzińska, M., Masewicz, Ł., Jarzębska, M., Nowakowski, P.T., Makiej, A., Kazemian, P., Drobnik, P., Stachowiak, B., Jarzębski, M., & Pratap-Singh, A. (2021). Multidisciplinary studies of folk medicine “Five Thieves’ Oil” (Olejek Pięciu Złodziei) components. Molecules, 26(10), 2931. ◄◄ To meet the growing interest in natural antibacterial agents, we evaluated the physicochemical and biological properties of the folk medicine known as “five thieves’ oil” (Polish name: olejek pięciu złodziei). Five thieves’ oil consists of a mixture of five oils: rosemary, lemon, clove, eucalyptus, and cinnamon. In this study, we performed gas chromatography, FTIR, and UV–vis spectroscopic analysis, as well as L-a-b color tests, contact angle determination, and surface tension determination. To verify its antibacterial activity, the metabolic activity and changes in cell membrane permeability of bacteria of the g...

FEMS Microbiology Letters

The oleaginous yeast Rhodosporidium diobovatum is a poorly described producer of carotenoids and ... more The oleaginous yeast Rhodosporidium diobovatum is a poorly described producer of carotenoids and may be of interest in biotechnology. This study investigated the effects of culture medium and illumination on effective carotenoid production by R. diobovatum IMB Y-5023. Yeast was cultured on carrot, bran extract, and modified yeast malt (YM) medium at illuminations in the 0-5000 lx range. Biomass, total carotenoids and their profile were assessed after cultivation. In order to compare samples, cluster analysis and principal component analysis were used to visualize the relationships between the variables and samples. Results show that it is not illumination but culture medium that is the main factor determining the quantity and proportions of carotenoids produced by R. diobovatum IMB Y-5023. The yeast grew and produced pigments throughout the tested range of illumination intensity. The modified YM medium was optimal for carotenogenesis. In cultures on that medium, the highest carotenoid yields of 24.98 mg g −1 dry cell weight and 77 mg L −1 were recorded. It was found that this yeast is capable of assimilating oligosaccharides and can grow and produce carotenoids in low-glucose media containing DP3 and DP4. Moreover R. diobovatum IMB Y-5023 produced lycopene as the main pigment independently of the culture conditions.

International Journal of Dairy Technology

Carbohydrate Polymers, 2016

Some ß-glucans can be easily extracted from Basidiomycete mushrooms but commonly used extraction ... more Some ß-glucans can be easily extracted from Basidiomycete mushrooms but commonly used extraction procedures are not satisfactory. A simultaneous method for acid extraction and deproteinization in the case of Pleurotus ostreatus was developed using response surface methodology. The optimized extraction conditions proposed here (30°C, 3.8% HCl, 300min, stirring) allow for the simultaneous extraction and deproteinization of polysaccharides. Additionally, the acid extraction yield was 7 times greater than that of hot water extraction. The combined enzymatic digestion with lyticase, ß-glucanase, exo-1,3-ß-d-glucanase, and ß-glucosidase results elucidated that an extract containing ß-1,3-ß-1,6-ß-1,4-glucan. The gel permeation chromatography (GPC) results showed that the two glucan fractions obtained do not contain linked proteins. The weight average molecular weight of the first fraction (Mw=1137kDa) was 60 times higher than that of the second fraction (Mw=19kDa).

Zywnosc Nauka Technologia Jakosc, 2006

Zywnosc: Nauka, Technologia, Jakosc

The study investigated the effect of a Bacillus globisporus strain and produced super-natants on ... more The study investigated the effect of a Bacillus globisporus strain and produced super-natants on growth of selected plant pathogens, i.e. Alternaria alternata, Botrytis cinerea, Clado-sporium spp., Trichothecium roseum, Fusarium oxysporum, F. solani, Trichoderma viride, Rhi-zoctonia solani, Sclerotinia sclerotiorum, Verticillium dahliae. The highest fungistatic activity of bacterial cultures was found against R. solani, S. sclerotiorum, B. cinerea and T. roseum, whereas in case of the supernatant, it was found against R. solani and S. sclerotiorum. At identical concentrations , the fungistatic activity of B. globisporus culture appeared stronger than that of the super-natant. No significant effect of bacterial culture concentration in the medium was observed. Growth of Cladosporium spp., V. dahliae, T. viride, and A. alternata was found both after seven and 10 days of the experiment. In case of the supernatant, this situation was observed only for F. oxysporum, after seven days.

Zywnosc: Nauka, Technologia, Jakosc

S t r e s z c z e n i e Astaksantyna naley do grupy barwników karotenoidowych. Znajduje ona powsz... more S t r e s z c z e n i e Astaksantyna naley do grupy barwników karotenoidowych. Znajduje ona powszechne zastosowanie jako niezbdny składnik pasz w przemysłowej hodowli łososi, pstrgów i krewetek, nadajc tkankom tych zwierzt podane przez konsumenta, charakterystyczne róowoczerwone zabarwienie. Ponadto, astaksantyna charakteryzuje si du aktywnoci przeciwutleniajc, najwysz wród znanych karotenoidów i 100-500-krotnie wysz w porównaniu z α-tokoferolem. Wród preparatów astaksantyny, wystpujcych na wiatowym rynku, 95% zawiera barwnik syntetyczny-mniej stabilny od astaksantyny pozyskiwanej ze ródeł naturalnych. Ograniczony zakres stosowania astaksantyny naturalnej wynika z kosztów jej otrzymywania, głównie na drodze syntezy mikrobiologicznej z udziałem alg Haematococcus pluvialis. Potencjalnym ródłem astaksantyny naturalnej s drode Phaffia rhodozyma. Jako ewentualne przemysłowe ródło tego barwnika wykazuj one wiele zalet. Przede wszystkim astaksantyna jest głównym produkowanym przez te drode...

The effect of pre-culturing of Pleurotus ostreatus in wheat bran extract after consequent heat tr... more The effect of pre-culturing of Pleurotus ostreatus in wheat bran extract after consequent heat treatment (5 min 100°C) and without it on the growth rate of the yeast Rhodosporidium diobovatum and Xanthophyllomyces dendrorhous was investigated. It was found that the pre-cultivation of Pleurotus ostreatus from 2 to 12 days thereafter depressed the yeast growth rate as compared to the base culture medium. In that case, if Pleurotus ostreatus was precultured in the base medium, and then treated by heat, Rhodosporidium diobovatum and Xanthophyllomyces dendrorhous growth rate increased by 60-80% as compared with the base medium cultivation. It is shown that the composition of the base medium after culturing of Pleurotus ostreatus and consequent heat treatment changed greatly, in particular glucose content increased. It has been shown that the presence of lipid components in natural nutrient media inhibits yeast growth rate.

Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists, 2013

Astaxanthin is a xanthophill pigment with commercial application in the aquaculture, pharmaceutic... more Astaxanthin is a xanthophill pigment with commercial application in the aquaculture, pharmaceutical, food and cosmetic industries. The red yeast Xanthophyllomyces dendrorhous is one of the most promising microorganisms for its industrial production. However, astaxanthin content in wild yeast strains is low. Pigment production by X. dendrorhous can be improved by mutagenesis. The aim of the study was to assess the efficiency of four mutagens: UV radiation, benomyl, ethyl methanesulfonate and ethidium bromide in generating asthaxanthin-hyperproducing strains of the yeast Xanthophyllomyces dendrorhous DSM 5626. Mutations with benomyl, ethidium bromide and UV radiation generated a group of hyperpigmented mutants exhibiting increases up to 100% in astaxanthin content. Ethyl methanesulfonate turned out to be useless in this respect.

Indian Journal of Microbiology, 2012

The study evaluated the effect of media based on plant extracts: potato, carrot and barley malt b... more The study evaluated the effect of media based on plant extracts: potato, carrot and barley malt broth, on growth and astaxanthin synthesis by yeast Xanthophyllomyces dendrorhous DSM 5626 and its mutants. The carrot medium promoted carotenogenesis most effectively. In cultures on this medium the highest volumetric and cellular concentrations of astaxanthin were recorded for four out of five tested strains. Also the share of astaxanthin in the total carotenoids produced by the tested strains was the highest.

Food Science and Biotechnology, 2013

The aim of the study was to select optimal illumination for Xanthophyllomyces dendrorhous DSM 562... more The aim of the study was to select optimal illumination for Xanthophyllomyces dendrorhous DSM 5626 and its mutants: 26UV, 10BE, 13B, and 34B for astaxanthin production. The strains were cultivated on YM medium at an illumination of 0-5,000 lx. The highest yields of astaxanthin were found in cultures at 600 lx. The maximum pigment production for the parental strain was 0.95 mg/L and 0.19 g/kg dry cell weight (DCW), while for mutants 1.23-1.51 mg/L and 0.34-0.39 g/kg DCW. It was calculated that the application of adequate values of illumination (within the range of 670-718 lx), determined on the basis of regression models, may improve astaxanthin production by approximately 5-15%, depending on the strain. This was confirmed in the verification experiments for the parental strain and mutants: 10BE, 13B, 26UV, but for 34B at 718 lx the volumetric astaxanthin production was found to be higher than predicted by the model.

... Antioxidant potential of carotenoid extracts from Phaffia rhodozyma mutants in linoleic acid ... more ... Antioxidant potential of carotenoid extracts from Phaffia rhodozyma mutants in linoleic acid emulsion Barbara Stachowiak, Anna Gramza-Michałowska, Jacek Nowak, Julita Reguła Wydział Nauk o Żywności i Żywieniu, Uniwersytet Przyrodniczy w Poznaniu Introduction. ...