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Papers by Bartłomiej Grygorcewicz

Microbial Drug Resistance, Nov 1, 2018

AIM The aim of the study was to investigate possible synergistic effects between several selected... more AIM The aim of the study was to investigate possible synergistic effects between several selected, commercially available essential oils and gentamicin against extended-spectrum β-lactamase (ESBL)-producing and New Delhi metallo-β-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae isolates. MATERIALS AND METHODS ESBLs production was confirmed by double-disk synergy test. Isolates positive for blaNDM-1 gene were found among the tested strains. K. pneumoniae ATCC® BAA-1705™ strain was used as a control. The checkerboard method was applied to assess the synergistic and additive action of nine essential oils: caraway, fennel, peppermint, geranium, basil, clove, thyme, clary sage, and lavender, respectively, in combination with gentamicin. RESULTS Our results indicated that peppermint oil combined with gentamicin showed synergistic activity against both control, ESBL-producing and NDM-1-producing isolates. Caraway essential oil demonstrated synergy with gentamicin toward ESBL-producing and additionally gentamicin-resistant strains. The additive effect was observed for gentamicin combined with thyme, fennel, basil, and clary sage. CONCLUSIONS Because of their synergistic activity with gentamicin, peppermint, and caraway oils in particular, can be considered as an alternative or an addition for the control of infections with limited therapeutic options due to multidrug resistance.

Postępy Mikrobiologii. Suplement, 2017

Microorganisms, Dec 29, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Archives of Microbiology, Jul 14, 2022

The influence of microbiota on the human body is currently the subject of many studies. The compo... more The influence of microbiota on the human body is currently the subject of many studies. The composition of bacteria colonizing the gastrointestinal tract varies depending on genetic make-up, lifestyle, use of antibiotics or the presence of diseases. The diet is also important in the species diversity of the microbiota. This study is an analysis of the relationships between physical activity, diet, and the microbiota of the gastrointestinal tract in athletes. This review shows the differences in the microbial composition in various sports disciplines, the influence of probiotics on the microbiome, the consequence of which may be achieved even better sports results. Physical activity increases the number of bacteria, mainly of the Clostridiales order and the genus: Lactobacillus, Prevotella, Bacteroides, and Veillonella, and their number varies depending on the sports discipline. These bacteria are present in athletes in sports that require a high VO 2 max. The players' diet also influences the composition of the microbiota. A diet rich in dietary fiber increases the amount of Lactobacillus or Bifidobacterium bacteria, probiotic microorganisms, which indicates the need to supplement the diet with probiotic preparations. It is impossible to suggest an unambiguous answer to how the microbiota of the gastrointestinal tract changes in athletes and requires further analyzes.

Microorganisms

Phage–antibiotic combination-based protocols are presently under heightened investigation. This p... more Phage–antibiotic combination-based protocols are presently under heightened investigation. This paradigm extends to engagements with bacterial biofilms, necessitating novel computational approaches to comprehensively characterize and optimize the outcomes achievable via these combinations. This study aimed to explore the Response Surface Methodology (RSM) in optimizing the antibiofilm activity of bacteriophage–antibiotic combinations. We employ a combination of antibiotics (gentamicin, meropenem, amikacin, ceftazidime, fosfomycin, imipenem, and colistin) alongside the bacteriophage vB_AbaP_AGC01 to combat Acinetobacter baumannii biofilm. Based on the conducted biofilm challenge assays analyzed using the RSM, the optimal points of antibiofilm activity efficacy were effectively selected by applying this methodology, enabling the quantifiable mathematical representations. Subsequent optimization showed the synergistic potential of the anti-biofilm that arises when antibiotics are judic...

Cellular and Molecular Neurobiology

Stem cells have been the subject of research for years due to their enormous therapeutic potentia... more Stem cells have been the subject of research for years due to their enormous therapeutic potential. Most neurological diseases such as multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD) are incurable or very difficult to treat. Therefore new therapies are sought in which autologous stem cells are used. They are often the patient's only hope for recovery or slowing down the progress of the disease symptoms. The most important conclusions arise after analyzing the literature on the use of stem cells in neurodegenerative diseases. The effectiveness of MSC cell therapy has been confirmed in ALS and HD therapy. MSC cells slow down ALS progression and show early promising signs of efficacy. In HD, they reduced huntingtin (Htt) aggregation and stimulation of endogenous neurogenesis. MS therapy with hematopoietic stem cells (HSCs) inducted significant recalibration of pro-inflammator...

International Journal of Molecular Sciences

Bacterial biofilms generally contribute to chronic infections, including wound infections. Due to... more Bacterial biofilms generally contribute to chronic infections, including wound infections. Due to the antibiotic resistance mechanisms protecting bacteria living in the biofilm, they are a serious problem in the wound healing process. To accelerate the wound healing process and avoid bacterial infection, it is necessary to select the appropriate dressing material. In this study, the promising therapeutic properties of alginate lyase (AlgL) immobilised on BC membranes for protecting wounds from Pseudomonas aeruginosa infection were investigated. The AlgL was immobilised on never dried BC pellicles via physical adsorption. The maximum adsorption capacity of AlgL was 6.0 mg/g of dry BC, and the equilibrium was reached after 2 h. The adsorption kinetics was studied, and it has been proven that the adsorption was consistent with Langmuir isotherm. In addition, the impact of enzyme immobilisation on bacterial biofilm stability and the effect of simultaneous immobilisation of AlgL and gent...

Advances in Applied Microbiology

Proteomics Clinical Applications, Aug 31, 2022

Microbial Drug Resistance

Alternative therapies against multidrug-resistant bacteria are widely investigated in the postant... more Alternative therapies against multidrug-resistant bacteria are widely investigated in the postantibiotic era. Polymicrobial biofilms formed by two or more species of bacteria or fungi pose an additional threat. The removal of such complex communities requires more effort and a multidirectional approach. In this study, the effectiveness of two bacteriophages vB_SauM-A (A) and vB_SauM-D (D) combined with ciprofloxacin was used to combat Staphylococcus aureus in the presence of Candida albicans both in liquid culture and biofilm. The results showed that phage-antibiotic synergy (PAS) led to the complete removal of S. aureus in liquid culture without bacterial population regrowth after 24 hours, and C. albicans enhanced this therapeutic effect. In a biofilm assay, C. albicans presence caused a decrease of bacterial eradication and a reduction of biofilm-specific activity (BSA). However, the strong effect of PAS was observed both in mono- and dual-species biofilm. Usage of phages and ciprofloxacin (1 mg/L) caused a 90% reduction of BSA of mono-species biofilm and 69% of dual-species biofilm. Phages alone resulted in a decrease of 71% and 48%, and ciprofloxacin (1 mg/L) alone resulted in 45% and 23% reduction, respectively. The influence of C. albicans on the PAS effect against S. aureus presented in this study was not previously investigated.

Biochemical Engineering Journal

Advancements of Microbiology, 2017

Wpłynęło w lipcu, zaakceptowano w sierpniu 2017 r. 1. Wstęp. 2. Zasady dotyczące wprowadzania zmi... more Wpłynęło w lipcu, zaakceptowano w sierpniu 2017 r. 1. Wstęp. 2. Zasady dotyczące wprowadzania zmian taksonomicznych. 3. Przesłanki wprowadzania zmian taksonomicznych. 4. Zmiany w rzędzie Enterobacterales ord. nov. 5. Bazy danych a zmiany w taksonomii rzędu Enterobacterales ord. nov. 6. Podsumowanie Changes in the taxonomy of γ-Proteobacteria, modification of the order Enterobacteriales and novel families within Enterobacterales ord. nov.

PLOS ONE

Background Urinary tract infections (UTI) represent one of the most common contagious diseases in... more Background Urinary tract infections (UTI) represent one of the most common contagious diseases in humans. Uropathogenic Escherichia coli (UPEC) strains are recognized as the most frequent causative agent, and these express a range of virulence factors including the adhesins. Immune response to UPEC under immunosuppression has not been fully understood yet. Interleukin 1β (IL1β), 6 (IL6) and 17 (IL17) represent clinically relevant markers of inflammation. Aim The study aimed to investigate the interplay between UPEC genotype and hosts’ immune status in shaping local inflammatory response in the course of an UTI episode. The respective numbers of: 18 kidney recipients with UPEC UTI, 28 immunocompetent hosts with UPEC UTI and 29 healthy controls were involved. Urine IL1β, IL6, and IL17/creatinine ratios in relation to fimH, csgA, papC, tosA, and flu genes presence in UPEC isolated from the urine samples were analyzed. Apart from traditional statistics, also machine learning algorithms ...

International Journal of Molecular Sciences

Three-dimensionally-printed aortic templates are increasingly being used to aid in the modificati... more Three-dimensionally-printed aortic templates are increasingly being used to aid in the modification of stent grafts in the treatment of urgent, complex aortic disorders, often of an emergency nature. The direct contact between the aortic template and the stent graft implies the necessity of complete sterility. Currently, the efficacy of sterilizing aortic templates and the effect of sterilization on the geometry of tubular aortic models are unknown. A complex case of aortic arch dissection was selected to prepare a 3D-printed aortic arch template, which was then manufactured in six popular printing materials: polylactic acid (PLA), nylon, polypropylene (PP), polyethylene terephthalate glycol (PETG), and a rigid and flexible photopolymer resin using fused deposition modeling (FDM) and stereolithography (SLA). The 3D models were contaminated with Geobacillus stearothermophilus broth and Bacillus atrophaeus. The sterilization was performed using three different methods: heat (105 °C an...

Current Issues in Molecular Biology

Growing interest in bacteriophage research and use, especially as an alternative treatment option... more Growing interest in bacteriophage research and use, especially as an alternative treatment option for multidrug-resistant bacterial infection, requires rapid development of production methods and strengthening of bacteriophage activities. Bacteriophage adsorption to host cells initiates the process of infection. The rotating magnetic field (RMF) is a promising biotechnological method for process intensification, especially for the intensification of micromixing and mass transfer. This study evaluates the use of RMF to enhance the infection process by influencing bacteriophage adsorption rate. The RMF exposition decreased the t50 and t75 of bacteriophages T4 on Escherichia coli cells and vb_SauM_A phages on Staphylococcus aureus cells. The T4 phage adsorption rate increased from 3.13 × 10−9 mL × min−1 to 1.64 × 10−8 mL × min−1. The adsorption rate of vb_SauM_A phages exposed to RMF increased from 4.94 × 10−9 mL × min−1 to 7.34 × 10−9 mL × min−1. Additionally, the phage T4 zeta potent...

International Journal of Molecular Sciences

Problems connected with biofilm-related infections and antibiotic resistance necessitate the inve... more Problems connected with biofilm-related infections and antibiotic resistance necessitate the investigation and development of novel treatment strategies. Given their unique characteristics, one of the most promising alternatives to conventional antibiotics are bacteriophages. In the in vitro and in vivo larva model study, we demonstrate that phages vB_SauM-A, vB_SauM-C, and vB_SauM-D are effective antibiofilm agents. The exposure of biofilm to phages vB_SauM-A and vB_SauM-D led to 2–3 log reductions in the colony-forming unit number in most of the multidrug-resistant S. aureus strains. It was found that phage application reduced the formed biofilms independently of the used titer. Moreover, the study demonstrated that bacteriophages are more efficient in biofilm biomass removal and reduction in staphylococci count when compared to the antibiotics used. The scanning electron microscopy analysis results are in line with colony forming unit (CFU) counting but not entirely consistent wi...

Prostaglandins & Other Lipid Mediators

Diagnostics

The aim of our study was to evaluate the influence of asymptomatic infection and the occurrence o... more The aim of our study was to evaluate the influence of asymptomatic infection and the occurrence of symptomatic COVID-19 on specific biochemical, renal, and immune parameters—renalase, neutrophil gelatinase-associated lipocalin (NGAL) cystatin C (CysC), and creatinine—and their weekly fluctuations during a one-month observation period in COVID-19 patients admitted to hospital. The study involved 86 individuals: 30 patients with diagnosed COVID-19, 28 people with asymptomatic infection confirmed with IgG antibodies—the IG(+) group—and 28 individuals without any (IgG, IgE) anti-SARS-CoV-2 antibodies—the IG(−) group. In the COVID-19 group, blood was drawn four times: (1) on day 0/1 after admission to hospital (C1 group), (2) 7 days later (C7 group), (3) 14 days later (C14 group), and (4) 28 days later (C28 group). In the IG(−) and IG(+) groups, blood was drawn once. There were no significant differences in creatinine, Cys C, and uric acid between any of the analyzed groups. NGAL levels ...

Neurochemistry International

Oxidative stress is defined as the persistent imbalance between the activity of toxic reactive fo... more Oxidative stress is defined as the persistent imbalance between the activity of toxic reactive forms of both oxygen and nitrogen and the antioxidant defense. In low concentrations, they are essential for the proper functioning of the body. Still, their excessive amount contributes to the damage of the biomolecules, consequently leading to various pathologies of the organism. Due to the lipid-rich brain structure, enormous oxygen consumption, and the lack of a sufficient antioxidant barrier make it highly susceptible to oxidative imbalance. Hence, oxidative stress has been linked to various psychiatric disorders. These diseases include all behavioral, emotional, and cognitive abnormalities associated with a significant impediment to social life. Each of the diseases in question: Alzheimer's disease, schizophrenia, depression, and bipolar disorder, is characterized by excessive oxidative stress. Considerable damages to DNA, RNA, proteins, lipids, and mitochondrial dysfunction, are observed. All conditions show increased lipid peroxidation, which appears to be typical of psychiatric disorders because the brain contains large amounts of these types of molecules. In addition, numerous abnormalities in the antioxidant defense are noted, but the results of studies on the activity of antioxidant enzymes differ significantly. The most promising biomarkers seem to be GSH in Alzheimer's disease as an early-stage marker of the disease and thioredoxin in schizophrenia as a marker for therapy monitoring. Data from the literature are consistent with the decrease in antioxidants such as vitamin C, E, uric acid, albumin, etc. Despite these numerous inconsistencies, it seems that oxidative stress is present in the course of psychiatric diseases. Still, it cannot be conclusively determined whether it is the direct cause of development, a consequence of other abnormalities at the biochemical or molecular level, or the result of the disease itself.

Microbial Drug Resistance, Nov 1, 2018

Postępy Mikrobiologii. Suplement, 2017

Microorganisms, Dec 29, 2022

Archives of Microbiology, Jul 14, 2022

Microorganisms

Cellular and Molecular Neurobiology

International Journal of Molecular Sciences

Advances in Applied Microbiology

Proteomics Clinical Applications, Aug 31, 2022

Microbial Drug Resistance

Biochemical Engineering Journal

Advancements of Microbiology, 2017

PLOS ONE

International Journal of Molecular Sciences

Current Issues in Molecular Biology

International Journal of Molecular Sciences

Prostaglandins & Other Lipid Mediators

Diagnostics

Neurochemistry International

Slurry is considered one of the best fertilizers. Nevertheless, it may contain harmful bacteria. ... more Slurry is considered one of the best fertilizers. Nevertheless, it may contain harmful bacteria. Spreading it on the soil may contribute to transfer of these microorganisms in the environment. This could be avoided with effective biocontrol methods Bacteriophage sall{\_}v01 specific to several Salmonella enterica serovars was isolated from wastewater and characterized. The lytic activity of bacteriophage sall{\_}v01 showed its effectiveness for the growth reduction of Salmonella Enteritidis at the multiplicity of infection = 1, with latent periods = 25 min, and the burst size approx. 107 ± 8 new virions per cell after 45 min at 37 °C. Bacteriophage-mediated treatment of experimentally contaminated swine slurry resulted in 3.8 log CFU/mL reduction in quantity of Salmonella Enteritidis. Based on our results, phage sall{\_}v01 could be considered a potential biocontrol agent against Salmonella Enteritidis contamination in agriculture and animal production.

$Research paper thumbnail of Changes in the taxonomy of <math xmlns="http://www.w3.org/1998/Math/MathML"><semantics><mrow><mi>γ</mi></mrow><annotation encoding="application/x-tex">\gamma</annotation></semantics></math>γ-Proteobacteria, modification of the order Enterobacteriales and novel families within Enterobacterales ord. Nov.$

Prokaryotic diversity increases every year with each new described species. Since the first disco... more Prokaryotic diversity increases every year with each new described species. Since the first discoveries of microorganisms, researchers' endeavours are dedicated to the systematisation of all known living organisms in a consistent taxonomy. Originally based on morphology, in recent years modern taxonomy develops thanks to the implemenation of new discoveries in the fields of biochemistry and genetics. In the last thirty years, ribotyping was the leading technique used to classify microorganisms. Due to problems with the comparison of certain species, novel methods based on the analysis of proteins have been applied. In-depth analysis of Enterobacteriaceae family showed that its members are more dissimilar than previously thought, which eventually led to dividing this family into seven families and resulted in a change to the name of the order: from Enterobacteriales to Enterobacterales. These changes were applied in some biggest accessible databases. However, there are still many other which have not modified their taxonomy records to date. Such situation may lead to unnecessary confusion, which strengthens the necessity to create one, unified taxonomy which is approved by the whole scientific community.

$Research paper thumbnail of Preliminary Study on the Antibacterial Activity of Essential Oils Alone and in Combination with Gentamicin Against Extended-Spectrum <math xmlns="http://www.w3.org/1998/Math/MathML"><semantics><mrow><mi>β</mi></mrow><annotation encoding="application/x-tex">\beta</annotation></semantics></math>β-Lactamase-Producing and New Delhi Metallo-$\beta$-Lactamase-1-Producing Klebsiella pneumoniae Isolates$

Slurry management is a contemporary agricultural problem. Although liquid manure is a valuable fe... more Slurry management is a contemporary agricultural problem. Although liquid manure is a valuable fertiliser, it may pose a microbiological threat due to spreading of bacteria resistant to various antibiotics. Fermentation has been proposed as a solution to this problem, although it does not fully eliminate the antibiotic-resistant strains. The presence of gram-negative rods resistant to antimicrobials may cause an uncontrolled transmission of resistance factors and genes in the natural environment. Therefore, a biocontrol system is necessary to prevent this phenomenon. The aim of this study was to detect multidrug-resistant (MDR) bacteria and their lytic bacteriophages in raw swine slurry. For that purpose, MDR bacteria from the family Enterobacteriaceae were isolated and tested with bacteriophages using a modified detection method. The study suggests that the bacteriophages present in the slurry are active against MDR bacteria and possibly could be used as a biocontrol agent for reduction of these microorganisms in fertilisers of animal origin. In the scope of the results obtained, a novel approach for the evaluation of slurry based on the preselection method is proposed. Moreover, the study reports the first case of isolation of a bacteriophage active against Providencia alcalifaciens.

In this work, three-phase partitioning (TPP) was used for the purification of laccase from liquid... more In this work, three-phase partitioning (TPP) was used for the purification of laccase from liquid cultures of Trametes versicolor. For determining the optimal conditions of TPP process, parameters such as initial pH (6.5, 7.0, 7.5, 8.0), ammonium sulphate saturation (20{\%}-80{\%}) and the water phase to tert-butanol ratio (1:0.5, 1:1, 1:2), were analyzed. The best conditions with 73{\%} recovery and 24-fold purification was obtained with the use of 50{\%} saturation with ammonium sulphate, water phase to tert-butanol ratio of 1:1 and initial pH 7.0. The molecular mass of the purified laccase secreted by analyzed strain T. versicolor was found for ≈ 66 kDa. The results showed that TPP is an efficient method for the fractionation and purification of laccase obtained from liquid cultures of T. versicolor and it allows for obtaining the relatively pure enzyme without the use of time-consuming and costly chromatographic methods.

\textcopyright} 2017 Kamila Mijowska et al. In this study, UV-C light was tested as an alternativ... more \textcopyright} 2017 Kamila Mijowska et al. In this study, UV-C light was tested as an alternative method to inactivate microorganisms in the must of 'Regent' red grape cultivar. The control sample containing the microorganism diluted in a physiological NaCl solution was prepared to take into consideration different conditions of liquids, such as turbidity and colour. Additionally, the changes in the composition of polyphenol compounds in the 'Regent' must after UV-C exposure were evaluated. The viability of yeasts (Saccharomyces cerevisiae) and bacteria (Oenococcus oeni) significantly decreased with time; however, the highest decline was noted after the first hour of exposure. The polyphenol compound content was significantly lower after UV-C treatment and this was mainly the result of anthocyanin decomposition. The total content of flavan-3-ols and hydroxycinnamic acids and derivatives increased after irradiation.

Bakteriofagi jako wirusy specyficznie infekuj{\c{a}}ce bakterie znajduj{\c{a}} coraz szersze zast... more Bakteriofagi jako wirusy specyficznie infekuj{\c{a}}ce bakterie znajduj{\c{a}} coraz szersze zastosowanie w przemy{\'{s}}le rolno-spo{\.{z}}ywczym, dodatkowo mo{\.{z}}na je zaliczy{\'{c}} do klasy naturalnych {\'{s}}rodk{\'{o}}w przeciwbakteryjnych. Skuteczno{\'{s}}{\'{c}} fag{\'{o}}w eliminowaniu bakterii podatnych na infekcj{\c{e}} doprowadzi{\l}a do szybkiego rozwoju produkt{\'{o}}w bakteriofagowych przeznaczonych do ochrony {\.{z}}ywno{\'{s}}ci. Dodatkowo, wzrost {\'{s}}wiadomo{\'{s}}ci konsument{\'{o}}w, dotycz{\c{a}}cy stosowanych chemicznych konserwant{\'{o}}w sprzyja rozwojowi alternatywnych, naturalnych metod kontroli patogen{\'{o}}w przenoszonych przez {\.{z}}ywno{\'{s}}{\'{c}}. Niniejsza praca ma na celu przedstawienie bakteriofag{\'{o}}w jako narz{\c{e}}dzia s{\l}u{\.{z}}{\c{a}}cego do ochrony zdrowia i bezpiecze{\'{n}}stwa konsument{\'{o}}w.

Background: Pathogenicity of Shiga toxin-producing strains of E. coli (STEC) is caused by the Shi... more Background: Pathogenicity of Shiga toxin-producing strains of E. coli (STEC) is caused by the Shiga toxin that is encoded in the lambdoid bacteriophage genome. Objective: The aim of the study was to analyze the influence of hydrogen peroxide on the induction of bacteriophages carrying genes encoding Shiga toxin and to define their mutual interaction and activity against selected strains of STEC. Results: The studies confirmed that H202 is an effective inducer of lambdoid bacteriophages induction. It was also found that H202 induced lambdoid phages, carrying the stxi and stx2 genes, may function as helper phages for the defective phages from this group, located in the genome of the animal STEC strailns having stx2 genes or representing a double lysogens (stx2/stx2).