Leonard Baskin - Academia.edu (original) (raw)

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Papers by Leonard Baskin

Research paper thumbnail of The Master of Mary of Burgundy

The Journal of Aesthetics and Art Criticism, 1971

Research paper thumbnail of Prints

Research paper thumbnail of Flowers and Insects, Some Birds and a Pair of Spiders

World Literature Today, 1987

Research paper thumbnail of Cave Birds: An Alchemical Cave Drama

World Literature Today, 1979

... Cave birds: An alchemical cave drama. Post a Comment. CONTRIBUTORS: Author: Hughes,Ted (b. 19... more ... Cave birds: An alchemical cave drama. Post a Comment. CONTRIBUTORS: Author: Hughes,Ted (b. 1930, d. ----. Author: Baskin, Leonard (b. 1922, d. ----. PUBLISHER: Viking Press (New York). SERIES TITLE: YEAR: 1978. PUB TYPE: Book (ISBN 0670209279 ). ...

Research paper thumbnail of Genomic sequences capable of committing mouse and rat fibroblasts to adipogenesis

Nucleic Acids Research, 1993

The mouse Swiss 3T3-F442A/3T3-C2 cell system is well suited for the isolation of genes involved i... more The mouse Swiss 3T3-F442A/3T3-C2 cell system is well suited for the isolation of genes involved in commitment to adipogenesis. 3T3-F442A cells convert to adipocytes with high efficiency in response to confluence and insulin. The sister clonal line 3T3-C2 does not respond to these signals, but can convert to adipocytes when transfected with DNA from 3T3-F442A preadipocytes or from human fat. Human fat-tissue biopsy F046 DNA transfected into 3T3-C2 gave rise to fat foci after two rounds of transfection and selection. A cosmid library of a subclone of secondary transfectant 3T3-C2/FO46-1 was screened for the human repetitive Alu sequence. Five out of eight Alu + recombinant clones committed 3T3-C2 cells to adipogenesis. The adipose commitment (AC) activity of one cosmid, p18A4, was found to reside in two small, non-identical, subcloned sequences 1.2kb and 2.0kb in length, each separately able to commit 3T3-C2, precrisis mouse and rat fibroblasts and the multipotential C3H1OT1/2 cell line to adipogenesis. We conclude that commitment to adipogenesis can be effected in vitro with high efficiency by transfection of specific sequences into a variety of host cells.

Research paper thumbnail of Evidence that oocyte maturation induced by an oncogenic ras-p21 protein and insulin is mediated by overlapping yet distinct mechanisms

Experimental Cell Research, 1992

We have recently shown that a peptide (residues 35-47) from a functional region of the ras p21 pr... more We have recently shown that a peptide (residues 35-47) from a functional region of the ras p21 protein, thought to be involved in the binding of p21 to GTPase activating protein, the antibiotic azatyrosine, known to induce the ras-recision gene, and the selective protein kinase C inhibitor, CGP 41,251, all inhibit oncogenic p21 protein-induced maturation of oocytes in a dose-dependent manner. We now show that these three agents only partially inhibit insulin-induced oocyte maturation, known to be dependent on activation of cellular p21 protein. On the other hand, the anti-p21 protein antibody Y13-259 completely inhibits both insulin- and oncogenic p21 protein-induced maturation as does a tetrapeptide, CVIM, known to block the enzyme farnesyl transferase which covalently attaches the farnesyl moiety to the p21 protein allowing it to attach to the cell membrane. Our results suggest that while the oncogenic and insulin-activated normal p21 proteins share certain elements of their signal transduction pathways in common, these pathways diverge and allow for selective inhibition of the oncogenic pathway.

Research paper thumbnail of The Master of Mary of Burgundy

The Journal of Aesthetics and Art Criticism, 1971

Research paper thumbnail of Prints

Research paper thumbnail of Flowers and Insects, Some Birds and a Pair of Spiders

World Literature Today, 1987

Research paper thumbnail of Cave Birds: An Alchemical Cave Drama

World Literature Today, 1979

... Cave birds: An alchemical cave drama. Post a Comment. CONTRIBUTORS: Author: Hughes,Ted (b. 19... more ... Cave birds: An alchemical cave drama. Post a Comment. CONTRIBUTORS: Author: Hughes,Ted (b. 1930, d. ----. Author: Baskin, Leonard (b. 1922, d. ----. PUBLISHER: Viking Press (New York). SERIES TITLE: YEAR: 1978. PUB TYPE: Book (ISBN 0670209279 ). ...

Research paper thumbnail of Genomic sequences capable of committing mouse and rat fibroblasts to adipogenesis

Nucleic Acids Research, 1993

The mouse Swiss 3T3-F442A/3T3-C2 cell system is well suited for the isolation of genes involved i... more The mouse Swiss 3T3-F442A/3T3-C2 cell system is well suited for the isolation of genes involved in commitment to adipogenesis. 3T3-F442A cells convert to adipocytes with high efficiency in response to confluence and insulin. The sister clonal line 3T3-C2 does not respond to these signals, but can convert to adipocytes when transfected with DNA from 3T3-F442A preadipocytes or from human fat. Human fat-tissue biopsy F046 DNA transfected into 3T3-C2 gave rise to fat foci after two rounds of transfection and selection. A cosmid library of a subclone of secondary transfectant 3T3-C2/FO46-1 was screened for the human repetitive Alu sequence. Five out of eight Alu + recombinant clones committed 3T3-C2 cells to adipogenesis. The adipose commitment (AC) activity of one cosmid, p18A4, was found to reside in two small, non-identical, subcloned sequences 1.2kb and 2.0kb in length, each separately able to commit 3T3-C2, precrisis mouse and rat fibroblasts and the multipotential C3H1OT1/2 cell line to adipogenesis. We conclude that commitment to adipogenesis can be effected in vitro with high efficiency by transfection of specific sequences into a variety of host cells.

Research paper thumbnail of Evidence that oocyte maturation induced by an oncogenic ras-p21 protein and insulin is mediated by overlapping yet distinct mechanisms

Experimental Cell Research, 1992

We have recently shown that a peptide (residues 35-47) from a functional region of the ras p21 pr... more We have recently shown that a peptide (residues 35-47) from a functional region of the ras p21 protein, thought to be involved in the binding of p21 to GTPase activating protein, the antibiotic azatyrosine, known to induce the ras-recision gene, and the selective protein kinase C inhibitor, CGP 41,251, all inhibit oncogenic p21 protein-induced maturation of oocytes in a dose-dependent manner. We now show that these three agents only partially inhibit insulin-induced oocyte maturation, known to be dependent on activation of cellular p21 protein. On the other hand, the anti-p21 protein antibody Y13-259 completely inhibits both insulin- and oncogenic p21 protein-induced maturation as does a tetrapeptide, CVIM, known to block the enzyme farnesyl transferase which covalently attaches the farnesyl moiety to the p21 protein allowing it to attach to the cell membrane. Our results suggest that while the oncogenic and insulin-activated normal p21 proteins share certain elements of their signal transduction pathways in common, these pathways diverge and allow for selective inhibition of the oncogenic pathway.

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