Beatriz Cordenunsi - Academia.edu (original) (raw)

Papers by Beatriz Cordenunsi

Brazilian Journal of Pharmaceutical Sciences, 2010

Acta Scientiarum-technology, Sep 1, 2018

Food Analytical Methods, Aug 20, 2014

Journal of Biotechnology, Feb 1, 1985

Radiation Physics and Chemistry, Sep 1, 2013

ABSTRACT This work investigated the effects of irradiation (0, 1 and 2 kGy) on the content of bio... more ABSTRACT This work investigated the effects of irradiation (0, 1 and 2 kGy) on the content of bioactive compounds such as vitamin C and carotenoids with provitamin A activity in arugula during the storage at 5±1 °C for up to 13 and 16 days, respectively. The vitamin C content decreased in non-irradiated as well as irradiated (1 and 2 kGy) samples during the storage period. On the other hand, no significant change in the content of carotenoids with provitamin A activity was observed after irradiation or storage period. Thus, the irradiation had minimal detrimental effects on the contents of carotenoids in arugula.

World Academy of Science, Engineering and Technology, International Journal of Nutrition and Food Engineering, 2015

Food Science and Technology International, 1994

Physico-chemical characterization and bioactive compounds of blackberry fruits (Rubus sp.) grown ... more Physico-chemical characterization and bioactive compounds of blackberry fruits (Rubus sp.) grown in Brazil Caracterização físico-química e de compostos bioativos em amora-preta (Rubus sp.) cultivada no Brasil

Revista Brasileira De Ciencias Farmaceuticas, 2004

Acta horticulturae, Apr 1, 2013

During fruit development, banana accumulates starch, which is then degraded during ripening. This... more During fruit development, banana accumulates starch, which is then degraded during ripening. This degradation provides precursors for sucrose and volatile compounds, responsible for sweetness and the characteristic aroma of the fruit. Starch metabolism may be influenced by the phytosanitary status of the plant. Black leaf streak disease (BLSD), caused by the pathogenic fungus Mycosphaerella fijiensis, is one of the most important diseases of banana. BLSD causes yield loss and has a strong effect on postharvest fruit quality. The aim of this study was to evaluate differences between the starch and sugars contents of fruits from a BLSD-infested plot and a fungicide-treated (control) plot. Starch and sugar contents were determined in fruits at two different physiological ages, i.e., early and late fruit harvest. Results show that BLSD may affect starch metabolism in a way similar to the advance of physiological age.

Acta horticulturae, Apr 1, 2013

Banana fruits are harvested and marketed in the green stage. Green Life (GL) is the number of day... more Banana fruits are harvested and marketed in the green stage. Green Life (GL) is the number of days between harvest and initiation of the ripening process, representing the time available to commercialization. Sigatoka leaf spot disease (SLSD), caused by the fungus Mycosphaerella musicola, is one of the main foliar diseases of banana production that leads to early ripening of the fruit, thus reducing the GL. The aim of this work was to determine whether removal of necrotic leaves could limit the effect of SLSD on fruit GL. Plants were classified according to four SLSD infestation levels, based on estimation of necrotic leaf area at flowering. GL of fruits was measured at 13°C, simulating the storage conditions during shipping, using two different treatments: plants with no leaf removal and plants with necrotic leaves removal (1 month before harvest). Higher SLSD infestation levels were associated with reduced GL values, reaching almost zero for the highest infestation level when leaves were not removed. However, with removal of necrotic leaves, GL increased over that of the control treatment (no leaf removal), and GL reached values greater than 40 days even for the highest infestation levels. Analyses of variance followed by Newman-Keuls tests (5% threshold) were conducted to compare different infestation levels among treatments. The removal of necrotic leaves 1 month before harvest considerably limited and, in some cases, suppressed the effect of SLSD on fruit GL at disease severity levels as high as 45% at flowering. In conclusion, pre-harvest removal of M. musicola-infected leaves represents a simple and effective cultural practice that may facilitate increased production of exportable bananas, when chemical control is not desired, not possible, or not efficient.

Plant Foods for Human Nutrition, Jul 6, 2011

Journal of Agricultural and Food Chemistry, Jul 26, 2008

In Arabidopsis (Arabidopsis thaliana) leaves, starch is synthesized during the day and degraded a... more In Arabidopsis (Arabidopsis thaliana) leaves, starch is synthesized during the day and degraded at night to fuel growth and metabolism. Starch is degraded primarily by b-amylases, liberating maltose, but this activity is preceded by glucan phosphorylation and is accompanied by dephosphorylation. A glucan phosphatase family member, LIKE SEX4 1 (LSF1), binds starch and is required for normal starch degradation, but its exact role is unclear. Here, we show that LSF1 does not dephosphorylate glucans. The recombinant dual specificity phosphatase (DSP) domain of LSF1 had no detectable phosphatase activity. Furthermore, a variant of LSF1 mutated in the catalytic cysteine of the DSP domain complemented the starch-excess phenotype of the lsf1 mutant. By contrast, a variant of LSF1 with mutations in the carbohydrate binding module did not complement lsf1. Thus, glucan binding, but not phosphatase activity, is required for the function of LSF1 in starch degradation. LSF1 interacts with the b-amylases BAM1 and BAM3, and the BAM1-LSF1 complex shows amylolytic but not glucan phosphatase activity. Nighttime maltose levels are reduced in lsf1, and genetic analysis indicated that the starchexcess phenotype of lsf1 is dependent on bam1 and bam3. We propose that LSF1 binds b-amylases at the starch granule surface, thereby promoting starch degradation. INTRODUCTION Starch, the major storage carbohydrate in plants, accumulates in leaves, as well as the storage tissues of seeds, tubers, and roots. In leaves, starch is synthesized in chloroplasts during the day from photo-assimilated carbon and is degraded at night to fuel metabolism in the dark. Starch consists of two glucan polymers, amylopectin and amylose. In amylopectin (the major polymer), a-1,4-linked glucan chains are connected to each other by a-1,6 linkages (branch points) to form a racemose, tree-like structure. Adjacent glucan chains in amylopectin form double helices and adopt a stable, semicrystalline lamellar structure. By contrast, amylose is essentially linear and is thought to occupy the spaces between the crystalline regions of amylopectin (Buléon et al., 1998; Zeeman et al., 2010; Pfister and Zeeman, 2016). A number of different enzymes are required for starch degradation in Arabidopsis (Arabidopsis thaliana) leaves (Zeeman et al., 2010). The first step is the phosphorylation of a small proportion of the glucosyl residues at the granule surface by the enzymes GLUCAN, WATER DIKINASE (GWD) and PHOSPHOGLUCAN, WATER DIKINASE (PWD), which phosphorylate glucan chains in the C6 and C3 positions, respectively (Baunsgaard et al., 2005; Kötting et al., 2005; Ritte et al., 2006). Phosphorylation is believed to disrupt the crystalline packing of the amylopectin, allowing hydrolytic enzymes access to the glucan chains (Edner et al., 2007; Hejazi et al., 2008). The major class of glucan hydrolase that participates in leaf starch degradation at night is b-amylase, an exoamylase that removes maltose from the non-reducing ends of the a-1,4-linked glucan chains (

Food Science and Technology, May 1, 1999

Food irradiation is one of the most promising treatments that can be utilized for fruits desinfes... more Food irradiation is one of the most promising treatments that can be utilized for fruits desinfestation and extension of shelf life. The authors studied the influence of 0,5 kGy of Gamma irradiation on the soluble carbohydrates composition of papaya (Carica papaya L. cv. Solo) fruit, and on sucrose metabolizing enzymes: sucrose synthase (SS), sucrose-phosphate synthase (SPS), acid and neutral invertases activities, during ripening. The results demonstred that ethylene production, total soluble sugars, sucrose content, and sucrose-phosphate synthase and invertases activities were affected by irradiation, but not respiration, glucose and fructose content, and SS activity.

Brazilian Journal of Pharmaceutical Sciences, 2010

Acta Scientiarum-technology, Sep 1, 2018

Food Analytical Methods, Aug 20, 2014

Journal of Biotechnology, Feb 1, 1985

Radiation Physics and Chemistry, Sep 1, 2013

ABSTRACT This work investigated the effects of irradiation (0, 1 and 2 kGy) on the content of bio... more ABSTRACT This work investigated the effects of irradiation (0, 1 and 2 kGy) on the content of bioactive compounds such as vitamin C and carotenoids with provitamin A activity in arugula during the storage at 5±1 °C for up to 13 and 16 days, respectively. The vitamin C content decreased in non-irradiated as well as irradiated (1 and 2 kGy) samples during the storage period. On the other hand, no significant change in the content of carotenoids with provitamin A activity was observed after irradiation or storage period. Thus, the irradiation had minimal detrimental effects on the contents of carotenoids in arugula.

World Academy of Science, Engineering and Technology, International Journal of Nutrition and Food Engineering, 2015

Food Science and Technology International, 1994

Physico-chemical characterization and bioactive compounds of blackberry fruits (Rubus sp.) grown ... more Physico-chemical characterization and bioactive compounds of blackberry fruits (Rubus sp.) grown in Brazil Caracterização físico-química e de compostos bioativos em amora-preta (Rubus sp.) cultivada no Brasil

Revista Brasileira De Ciencias Farmaceuticas, 2004

Acta horticulturae, Apr 1, 2013

During fruit development, banana accumulates starch, which is then degraded during ripening. This... more During fruit development, banana accumulates starch, which is then degraded during ripening. This degradation provides precursors for sucrose and volatile compounds, responsible for sweetness and the characteristic aroma of the fruit. Starch metabolism may be influenced by the phytosanitary status of the plant. Black leaf streak disease (BLSD), caused by the pathogenic fungus Mycosphaerella fijiensis, is one of the most important diseases of banana. BLSD causes yield loss and has a strong effect on postharvest fruit quality. The aim of this study was to evaluate differences between the starch and sugars contents of fruits from a BLSD-infested plot and a fungicide-treated (control) plot. Starch and sugar contents were determined in fruits at two different physiological ages, i.e., early and late fruit harvest. Results show that BLSD may affect starch metabolism in a way similar to the advance of physiological age.

Acta horticulturae, Apr 1, 2013

Banana fruits are harvested and marketed in the green stage. Green Life (GL) is the number of day... more Banana fruits are harvested and marketed in the green stage. Green Life (GL) is the number of days between harvest and initiation of the ripening process, representing the time available to commercialization. Sigatoka leaf spot disease (SLSD), caused by the fungus Mycosphaerella musicola, is one of the main foliar diseases of banana production that leads to early ripening of the fruit, thus reducing the GL. The aim of this work was to determine whether removal of necrotic leaves could limit the effect of SLSD on fruit GL. Plants were classified according to four SLSD infestation levels, based on estimation of necrotic leaf area at flowering. GL of fruits was measured at 13°C, simulating the storage conditions during shipping, using two different treatments: plants with no leaf removal and plants with necrotic leaves removal (1 month before harvest). Higher SLSD infestation levels were associated with reduced GL values, reaching almost zero for the highest infestation level when leaves were not removed. However, with removal of necrotic leaves, GL increased over that of the control treatment (no leaf removal), and GL reached values greater than 40 days even for the highest infestation levels. Analyses of variance followed by Newman-Keuls tests (5% threshold) were conducted to compare different infestation levels among treatments. The removal of necrotic leaves 1 month before harvest considerably limited and, in some cases, suppressed the effect of SLSD on fruit GL at disease severity levels as high as 45% at flowering. In conclusion, pre-harvest removal of M. musicola-infected leaves represents a simple and effective cultural practice that may facilitate increased production of exportable bananas, when chemical control is not desired, not possible, or not efficient.

Plant Foods for Human Nutrition, Jul 6, 2011

Journal of Agricultural and Food Chemistry, Jul 26, 2008

In Arabidopsis (Arabidopsis thaliana) leaves, starch is synthesized during the day and degraded a... more In Arabidopsis (Arabidopsis thaliana) leaves, starch is synthesized during the day and degraded at night to fuel growth and metabolism. Starch is degraded primarily by b-amylases, liberating maltose, but this activity is preceded by glucan phosphorylation and is accompanied by dephosphorylation. A glucan phosphatase family member, LIKE SEX4 1 (LSF1), binds starch and is required for normal starch degradation, but its exact role is unclear. Here, we show that LSF1 does not dephosphorylate glucans. The recombinant dual specificity phosphatase (DSP) domain of LSF1 had no detectable phosphatase activity. Furthermore, a variant of LSF1 mutated in the catalytic cysteine of the DSP domain complemented the starch-excess phenotype of the lsf1 mutant. By contrast, a variant of LSF1 with mutations in the carbohydrate binding module did not complement lsf1. Thus, glucan binding, but not phosphatase activity, is required for the function of LSF1 in starch degradation. LSF1 interacts with the b-amylases BAM1 and BAM3, and the BAM1-LSF1 complex shows amylolytic but not glucan phosphatase activity. Nighttime maltose levels are reduced in lsf1, and genetic analysis indicated that the starchexcess phenotype of lsf1 is dependent on bam1 and bam3. We propose that LSF1 binds b-amylases at the starch granule surface, thereby promoting starch degradation. INTRODUCTION Starch, the major storage carbohydrate in plants, accumulates in leaves, as well as the storage tissues of seeds, tubers, and roots. In leaves, starch is synthesized in chloroplasts during the day from photo-assimilated carbon and is degraded at night to fuel metabolism in the dark. Starch consists of two glucan polymers, amylopectin and amylose. In amylopectin (the major polymer), a-1,4-linked glucan chains are connected to each other by a-1,6 linkages (branch points) to form a racemose, tree-like structure. Adjacent glucan chains in amylopectin form double helices and adopt a stable, semicrystalline lamellar structure. By contrast, amylose is essentially linear and is thought to occupy the spaces between the crystalline regions of amylopectin (Buléon et al., 1998; Zeeman et al., 2010; Pfister and Zeeman, 2016). A number of different enzymes are required for starch degradation in Arabidopsis (Arabidopsis thaliana) leaves (Zeeman et al., 2010). The first step is the phosphorylation of a small proportion of the glucosyl residues at the granule surface by the enzymes GLUCAN, WATER DIKINASE (GWD) and PHOSPHOGLUCAN, WATER DIKINASE (PWD), which phosphorylate glucan chains in the C6 and C3 positions, respectively (Baunsgaard et al., 2005; Kötting et al., 2005; Ritte et al., 2006). Phosphorylation is believed to disrupt the crystalline packing of the amylopectin, allowing hydrolytic enzymes access to the glucan chains (Edner et al., 2007; Hejazi et al., 2008). The major class of glucan hydrolase that participates in leaf starch degradation at night is b-amylase, an exoamylase that removes maltose from the non-reducing ends of the a-1,4-linked glucan chains (

Food Science and Technology, May 1, 1999

Food irradiation is one of the most promising treatments that can be utilized for fruits desinfes... more Food irradiation is one of the most promising treatments that can be utilized for fruits desinfestation and extension of shelf life. The authors studied the influence of 0,5 kGy of Gamma irradiation on the soluble carbohydrates composition of papaya (Carica papaya L. cv. Solo) fruit, and on sucrose metabolizing enzymes: sucrose synthase (SS), sucrose-phosphate synthase (SPS), acid and neutral invertases activities, during ripening. The results demonstred that ethylene production, total soluble sugars, sucrose content, and sucrose-phosphate synthase and invertases activities were affected by irradiation, but not respiration, glucose and fructose content, and SS activity.