Mathieu Belanger - Academia.edu (original) (raw)
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This study was conducted to develop tools for the evaluation of alternatives to antimicrobial gro... more This study was conducted to develop tools for the evaluation of alternatives to antimicrobial growth promoters to prevent necrotic enteritis in broiler chickens. A Clostridium perfringens infection model was carried out to assess the role of Eimeria infection and high wheat feed content as predisposing factors in broiler chickens for necrotic enteritis. Chickens were divided into 5 groups: Three groups were fed with a 45%, 35% or 25% wheat diet and 2 groups were fed with a 35% wheat diet with a 40 or 24 times the recommended dose of a live Eimeria vaccine. Chickens were challenged per os with a mixture of 3 Clostridium perfringens isolated from c1inical cases. After challenge, c1inical signs were observed and macroscopic lesion scoring was carried out. Only groups which received the Eimeria vaccine showed c1inical signs and macroscopic lesions in the jejunum corresponding to c1inical necrotic enteritis. Furthermore, the jejunum, ileum and cecal contents were collected for fecal coliforms, Lactobacillus, C. perfringens and total bacteria counts. On day 2 post-inoculation, C. perfringens counts in jejunum from the group receiving 24 times the anticoccidial dose were higher th an the control group. Important variations in counts were shown in cecum for the bacterial species that were studied. Eimeria infection with high wheat content in the meal predisposed broiler chickens to necrotic enteritis and that intestinal microflora modifications are observed after C. perfringens challenge. This model can be used to assess antimicrobial growth factors alternatives for preventing necrotic enteritis.
This study was conducted to develop tools for the evaluation of alternatives to antimicrobial gro... more This study was conducted to develop tools for the evaluation of alternatives to antimicrobial growth promoters to prevent necrotic enteritis in broiler chickens. A Clostridium perfringens infection model was carried out to assess the role of Eimeria infection and high wheat feed content as predisposing factors in broiler chickens for necrotic enteritis. Chickens were divided into 5 groups: Three groups were fed with a 45%, 35% or 25% wheat diet and 2 groups were fed with a 35% wheat diet with a 40 or 24 times the recommended dose of a live Eimeria vaccine. Chickens were challenged per os with a mixture of 3 Clostridium perfringens isolated from c1inical cases. After challenge, c1inical signs were observed and macroscopic lesion scoring was carried out. Only groups which received the Eimeria vaccine showed c1inical signs and macroscopic lesions in the jejunum corresponding to c1inical necrotic enteritis. Furthermore, the jejunum, ileum and cecal contents were collected for fecal coliforms, Lactobacillus, C. perfringens and total bacteria counts. On day 2 post-inoculation, C. perfringens counts in jejunum from the group receiving 24 times the anticoccidial dose were higher th an the control group. Important variations in counts were shown in cecum for the bacterial species that were studied. Eimeria infection with high wheat content in the meal predisposed broiler chickens to necrotic enteritis and that intestinal microflora modifications are observed after C. perfringens challenge. This model can be used to assess antimicrobial growth factors alternatives for preventing necrotic enteritis.