Benjamin Opawale - Academia.edu (original) (raw)

Papers by Benjamin Opawale

Research paper thumbnail of Antimicrobial and Phytochemical Screening of Stem Bark Extracts of Lovoa trichiliodes (Harm) and Trichilia heudelotii Planc (Harm)

World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences, Dec 26, 2016

Research paper thumbnail of A Survey of Physicochemical andBacteriological Quality of Pipe-Borne Water Used for Drinking in Rufus Giwa Polytechnic, Owo Ondo State, Nigeria

Greener Journal of Science, Engineering and Technological Research, 2013

Pipe borne water samples used for drinking in Rufus Giwa Polytechnic, Owo were analysed bacteriol... more Pipe borne water samples used for drinking in Rufus Giwa Polytechnic, Owo were analysed bacteriologically and physicochemically using standard methods. Four water samples were subjected to physicochemical analyses like PH, temperature, color, turbidity total hardness, dissolve solids, salinity, conductivity total solid, nitrate, sulphate, calcium, and sodium. These properties of the water samples were within the WHO/FAO standard for water except dissolve solids, nitrate chloride and conductivity. The bacteriological analysis was carried out to detect the total mesophillic, coliform count and subsequently, organisms present in the water samples. The total mesophillic count ranged between 2.6x10 4 to 3.3x10 4 cfu/ml while the total coliform count ranged between 1.1x10 3 to 1.6x10 3 cfu/ml. Both values were higher than the month standard limit for drinking water. The identified organisms include; Staphylococcus aureus, Citrobacter, sp, Proteus sp, E.coli, Bacillus sp, Pseudomonas sp, Enterobacter sp, klebsiella sp, Acinetobacter sp, Streptococcus sp and Serratia sp. 70% of the isolates were gram negative and most belonged to the family Enterobacteriacea. The data suggests the need for treatment of the water samples by the school authority and by simple boiling by the consumers.

Research paper thumbnail of Antimicrobial, free radical scavenging and cytotoxic activities of Khaya grandifoliola C.DC extracts

Malaysian Journal of Microbiology, 2016

Aims: Khaya grandifoliola C.DC is a plant used locally in Nigeria ethno medicine for remedy of va... more Aims: Khaya grandifoliola C.DC is a plant used locally in Nigeria ethno medicine for remedy of various disease conditions. However, there is little scientific evidence to support the therapeutic claims of the plant. Therefore, these investigations were conducted to determine the antimicrobial activity, antioxidant and cytotoxic potentials of the plant extracts. Methodology and results: In vitro antimicrobial activity of the leaf and stem bark extracts of K. grandifoliola against some human pathogens was done using agar diffusion method. The free radical scavenging activity and cytotoxic property of the plant materials were evaluated using 2, 2-diphenyl-1-pieryhydrazyl radical (DPPH) and brine shrimp lethality bioassay methods respectively. The yields of the plant material extracts ranged from 3.57±0.06 to 6.49±0.01% and 4.76±0.02 to 9.17±0.06% for the leaf and stem bark extracts respectively. The minimum inhibitory concentration (MIC) of KG-A and KG-E ranged from 2.5 to 200 mg/mL and recorded remarkable activity against the growth of Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Trichophyton rubrum and Aspergillus flavus. However, Strepcoccus pneumoniae, Staphylococcus epidermidis, Klebsiella pneumoniae, and T. rubrum were resistant to the KG-W. The plant extracts demonstrated high DPPH free radical scavenging activity when compared with ascorbic acid used as control in the assay and, also exhibited lethality against brine shrimp larvae with LC50 values ranging from: leaf extracts (0.67 to 1502 ppm) and stem bark extracts (0.91 to 1431 ppm). Conclusion, significance and impact of study: The results show that the KG-A and KG-E have great potentials as antimicrobial agent and may be used in the treatment of infectious diseases caused by the susceptible organisms.

Research paper thumbnail of Assessment of the physico-chemical characteristics and elemental properties of water and sediments from Owan river in Edo State, Nigeria

International Journal of Energy and Water Resources, 2021

The water quality of Owan river, a very important river in Ovia North-East Local Government Area ... more The water quality of Owan river, a very important river in Ovia North-East Local Government Area of Edo State, Nigeria was assessed. American Public Health Association (APHA) standard analytical procedures for water and sediment samples were used. Elemental composition of the sediment samples were determined by Energy Dispersive X-ray Fluorescence Spectrometer (EDXRF). Results obtained were statistically analysed (basic descriptive). In water, concentrations (mg/L) of nitrate—(2.65–21.55), sulphate—(58.6–80.0), calcium —(3.21–8.60), dissolved oxygen (5.5–6.53), total hardness (12.60–42.0), alkalinity (32.0–50.5), Cd (0.01), Cr (0.1–0.15), As (0.01–0.05), and Zn (1.4–2.04), electrical conductivity (32.0–38.0 µS/cm) and pH (6.10–6.85) were within environmental standards. However, Fe and Pb were slightly above the standards. In the sediment samples, Al, Si, Fe, and K are the most abundant elements. The highest values were detected as follows: Al—8.09 (SW7), Si—25.08 (SW5), Fe—16.72 (SW...

Research paper thumbnail of Antimicrobial and Phytochemical Screening of Stem Bark Extracts of Lovoa trichiliodes (Harm) and Trichilia heudelotii Planc (Harm)

World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences, 2016

Research paper thumbnail of Phytochemical Screening, Antifungal and Cytotoxic Activities of Trichilia heudelotii Planc (Harm)

International Journal of Sciences: Basic and Applied Research, 2015

This study was designed to evaluate the antifungal and cytotoxic activities as well as phytochemi... more This study was designed to evaluate the antifungal and cytotoxic activities as well as phytochemical screening of leaf and stem bark extracts (Aqueous, Acetone and Ethanol) of Trichilia heudelotii Planc Harm. against some human pathogenic fungi using standard methods. The average yield of the extracts ranged from 9.62±0.01 to 10.20±0.01 and 6.39±0.01 to 14.19±0.06 for the leaf and stem bark extracts respectively. Phytochemical screening revealed the presence of alkaloids, tannins, phlobatannins, phenols, anthraquinones, glycosides and flavonoids. Steroids and chalcones were absent in all the extracts. At the concentration of 50mg/ml, the highest zone of inhibition was exhibited by acetone leaf extract against Candida albicans ATCC10231 (17.00±0.58mm) followed by acetone and ethanol stem bark extracts against Trichophyton rubrum and Candida albicans exhibiting zone of inhibition of 16.00±0.00mm respectively. The aqueous leaf extracts showed the least activity of 6.00±0.00 in T. rubru...

Research paper thumbnail of Correction to: Assessment of the physico-chemical characteristics and elemental properties of water and sediments from Owan river in Edo State, Nigeria

International Journal of Energy and Water Resources

The publication of this article unfortunately contained mistakes. The given and family names of t... more The publication of this article unfortunately contained mistakes. The given and family names of the co-authors were not correct. The corrected names you can see above. The original article has been corrected.

Research paper thumbnail of Evaluation of bioactivity of stem bark extracts of Lovoa trichiliodes (Harm) and Trichilia heudelotii Planc (Harm)

GSC Biological and Pharmaceutical Sciences

This study evaluated the bioactivity of the stem bark extracts of Lovoa trichiliodes and Trichili... more This study evaluated the bioactivity of the stem bark extracts of Lovoa trichiliodes and Trichilia heudelotii using standard methods. The highest yield of 10.20% was obtained from the stem bark extract of T. heudelotii. Qualitative phytochemical examination of the plant extracts indicated the presence of different secondary metabolites which remarkably inhibited the growth of Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Salmonella typhii, Aspergillus flavus, Candida albicans and Candida glabrata. However, the cold water extract of L. trichiliodes exhibited no activity against the test pathogens. The highest mean (22.33±0.33 mm) zone of inhibition and minimum inhibitory concentration (MIC) of 2.5 mg/ml were exhibited by the acetone stem bark extract of L trichiliodes against B. subtilis ATCC6633. The results affirmed the traditional uses of the plants in the management and treatment of numerous diseases caused by the test pathogens.

Research paper thumbnail of Effects of Processing on the Yield and Physico-Chemical Properties of Cassava Products

Advanced Materials Research, 2007

... roots were processed into selected products( gari, 'pupuru', 'Lafun', chi... more ... roots were processed into selected products( gari, 'pupuru', 'Lafun', chips and starch) using unit operations such as peeling, washing, cutting, grating, dewatering(fermentation), smoking, granulated roasting(garifying), milling, packaging). The effects of processing on the yield ...

Research paper thumbnail of Antimicrobial and Phytochemical Screening of Stem Bark Extracts of Lovoa trichiliodes (Harm) and Trichilia heudelotii Planc (Harm)

World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences, Dec 26, 2016

Research paper thumbnail of A Survey of Physicochemical andBacteriological Quality of Pipe-Borne Water Used for Drinking in Rufus Giwa Polytechnic, Owo Ondo State, Nigeria

Greener Journal of Science, Engineering and Technological Research, 2013

Pipe borne water samples used for drinking in Rufus Giwa Polytechnic, Owo were analysed bacteriol... more Pipe borne water samples used for drinking in Rufus Giwa Polytechnic, Owo were analysed bacteriologically and physicochemically using standard methods. Four water samples were subjected to physicochemical analyses like PH, temperature, color, turbidity total hardness, dissolve solids, salinity, conductivity total solid, nitrate, sulphate, calcium, and sodium. These properties of the water samples were within the WHO/FAO standard for water except dissolve solids, nitrate chloride and conductivity. The bacteriological analysis was carried out to detect the total mesophillic, coliform count and subsequently, organisms present in the water samples. The total mesophillic count ranged between 2.6x10 4 to 3.3x10 4 cfu/ml while the total coliform count ranged between 1.1x10 3 to 1.6x10 3 cfu/ml. Both values were higher than the month standard limit for drinking water. The identified organisms include; Staphylococcus aureus, Citrobacter, sp, Proteus sp, E.coli, Bacillus sp, Pseudomonas sp, Enterobacter sp, klebsiella sp, Acinetobacter sp, Streptococcus sp and Serratia sp. 70% of the isolates were gram negative and most belonged to the family Enterobacteriacea. The data suggests the need for treatment of the water samples by the school authority and by simple boiling by the consumers.

Research paper thumbnail of Antimicrobial, free radical scavenging and cytotoxic activities of Khaya grandifoliola C.DC extracts

Malaysian Journal of Microbiology, 2016

Aims: Khaya grandifoliola C.DC is a plant used locally in Nigeria ethno medicine for remedy of va... more Aims: Khaya grandifoliola C.DC is a plant used locally in Nigeria ethno medicine for remedy of various disease conditions. However, there is little scientific evidence to support the therapeutic claims of the plant. Therefore, these investigations were conducted to determine the antimicrobial activity, antioxidant and cytotoxic potentials of the plant extracts. Methodology and results: In vitro antimicrobial activity of the leaf and stem bark extracts of K. grandifoliola against some human pathogens was done using agar diffusion method. The free radical scavenging activity and cytotoxic property of the plant materials were evaluated using 2, 2-diphenyl-1-pieryhydrazyl radical (DPPH) and brine shrimp lethality bioassay methods respectively. The yields of the plant material extracts ranged from 3.57±0.06 to 6.49±0.01% and 4.76±0.02 to 9.17±0.06% for the leaf and stem bark extracts respectively. The minimum inhibitory concentration (MIC) of KG-A and KG-E ranged from 2.5 to 200 mg/mL and recorded remarkable activity against the growth of Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Trichophyton rubrum and Aspergillus flavus. However, Strepcoccus pneumoniae, Staphylococcus epidermidis, Klebsiella pneumoniae, and T. rubrum were resistant to the KG-W. The plant extracts demonstrated high DPPH free radical scavenging activity when compared with ascorbic acid used as control in the assay and, also exhibited lethality against brine shrimp larvae with LC50 values ranging from: leaf extracts (0.67 to 1502 ppm) and stem bark extracts (0.91 to 1431 ppm). Conclusion, significance and impact of study: The results show that the KG-A and KG-E have great potentials as antimicrobial agent and may be used in the treatment of infectious diseases caused by the susceptible organisms.

Research paper thumbnail of Assessment of the physico-chemical characteristics and elemental properties of water and sediments from Owan river in Edo State, Nigeria

International Journal of Energy and Water Resources, 2021

The water quality of Owan river, a very important river in Ovia North-East Local Government Area ... more The water quality of Owan river, a very important river in Ovia North-East Local Government Area of Edo State, Nigeria was assessed. American Public Health Association (APHA) standard analytical procedures for water and sediment samples were used. Elemental composition of the sediment samples were determined by Energy Dispersive X-ray Fluorescence Spectrometer (EDXRF). Results obtained were statistically analysed (basic descriptive). In water, concentrations (mg/L) of nitrate—(2.65–21.55), sulphate—(58.6–80.0), calcium —(3.21–8.60), dissolved oxygen (5.5–6.53), total hardness (12.60–42.0), alkalinity (32.0–50.5), Cd (0.01), Cr (0.1–0.15), As (0.01–0.05), and Zn (1.4–2.04), electrical conductivity (32.0–38.0 µS/cm) and pH (6.10–6.85) were within environmental standards. However, Fe and Pb were slightly above the standards. In the sediment samples, Al, Si, Fe, and K are the most abundant elements. The highest values were detected as follows: Al—8.09 (SW7), Si—25.08 (SW5), Fe—16.72 (SW...

Research paper thumbnail of Antimicrobial and Phytochemical Screening of Stem Bark Extracts of Lovoa trichiliodes (Harm) and Trichilia heudelotii Planc (Harm)

World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences, 2016

Research paper thumbnail of Phytochemical Screening, Antifungal and Cytotoxic Activities of Trichilia heudelotii Planc (Harm)

International Journal of Sciences: Basic and Applied Research, 2015

This study was designed to evaluate the antifungal and cytotoxic activities as well as phytochemi... more This study was designed to evaluate the antifungal and cytotoxic activities as well as phytochemical screening of leaf and stem bark extracts (Aqueous, Acetone and Ethanol) of Trichilia heudelotii Planc Harm. against some human pathogenic fungi using standard methods. The average yield of the extracts ranged from 9.62±0.01 to 10.20±0.01 and 6.39±0.01 to 14.19±0.06 for the leaf and stem bark extracts respectively. Phytochemical screening revealed the presence of alkaloids, tannins, phlobatannins, phenols, anthraquinones, glycosides and flavonoids. Steroids and chalcones were absent in all the extracts. At the concentration of 50mg/ml, the highest zone of inhibition was exhibited by acetone leaf extract against Candida albicans ATCC10231 (17.00±0.58mm) followed by acetone and ethanol stem bark extracts against Trichophyton rubrum and Candida albicans exhibiting zone of inhibition of 16.00±0.00mm respectively. The aqueous leaf extracts showed the least activity of 6.00±0.00 in T. rubru...

Research paper thumbnail of Correction to: Assessment of the physico-chemical characteristics and elemental properties of water and sediments from Owan river in Edo State, Nigeria

International Journal of Energy and Water Resources

The publication of this article unfortunately contained mistakes. The given and family names of t... more The publication of this article unfortunately contained mistakes. The given and family names of the co-authors were not correct. The corrected names you can see above. The original article has been corrected.

Research paper thumbnail of Evaluation of bioactivity of stem bark extracts of Lovoa trichiliodes (Harm) and Trichilia heudelotii Planc (Harm)

GSC Biological and Pharmaceutical Sciences

This study evaluated the bioactivity of the stem bark extracts of Lovoa trichiliodes and Trichili... more This study evaluated the bioactivity of the stem bark extracts of Lovoa trichiliodes and Trichilia heudelotii using standard methods. The highest yield of 10.20% was obtained from the stem bark extract of T. heudelotii. Qualitative phytochemical examination of the plant extracts indicated the presence of different secondary metabolites which remarkably inhibited the growth of Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Salmonella typhii, Aspergillus flavus, Candida albicans and Candida glabrata. However, the cold water extract of L. trichiliodes exhibited no activity against the test pathogens. The highest mean (22.33±0.33 mm) zone of inhibition and minimum inhibitory concentration (MIC) of 2.5 mg/ml were exhibited by the acetone stem bark extract of L trichiliodes against B. subtilis ATCC6633. The results affirmed the traditional uses of the plants in the management and treatment of numerous diseases caused by the test pathogens.

Research paper thumbnail of Effects of Processing on the Yield and Physico-Chemical Properties of Cassava Products

Advanced Materials Research, 2007

... roots were processed into selected products( gari, 'pupuru', 'Lafun', chi... more ... roots were processed into selected products( gari, 'pupuru', 'Lafun', chips and starch) using unit operations such as peeling, washing, cutting, grating, dewatering(fermentation), smoking, granulated roasting(garifying), milling, packaging). The effects of processing on the yield ...