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Papers by Benson Oluwafemi

Research paper thumbnail of Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay

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Research paper thumbnail of An overview on antidiabetic medicinal plants having insulin mimetic property

Asian Pacific Journal of …, 2012

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Research paper thumbnail of Aldose reductase inhibitory activity and anti catraract potential of some traditionally acclaimed antidiabetic medicinal plants

Oriental Pharmacy and Experimental Medicine, 2009

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Research paper thumbnail of Antidiabetic Medicinal Plants as a Source of Alpha Glucosidase Inhibitors

Current diabetes …, 2010

The aim of this study is to collate all available data on antidiabetic plants that inhibit alpha ... more The aim of this study is to collate all available data on antidiabetic plants that inhibit alpha glucosidase, reported mainly by Medline (PubMed) these last years. In the present study, interest is focused on experimental researches conducted on hypoglycemic plants particularly those which show alpha glucosidase inhibitor activity alongside bioactive components. This study describes 47 species that belong to 29 families. The plant families, which enclose the species, studied most as inhibitors of alphaglucosidase, are Fabaceae (6 species.), Crassulaceae (3 species), Hippocrateacaea (3 species), Lamiaceae (3 species), and Myrtaceae (3 species), with most studied species being Salacia reticulata (Hippocrateaceae) and Morus alba (Moraceae). The study also covers natural products (active natural components and crude extracts) isolated from the medicinal plants which inhibit alpha glucosidase as reported this last decade. Many kinds of these isolated natural products show strong activity such as, Alkaloids, stilbenoids (polyphenol), triterpene, acids (chlorogenic acid, betulinic acid, syringic acid, vanillic acid, bartogenic acid, oleanolic acid, dehydrotrametenolic acid, corosolic acid, ellagic acid, ursolic acid, gallic acid), phytosterol, myoinositol, flavonoids, Flavonolignans, anthraquinones, anthrones, and xanthones, Feruloylglucosides, flavanone glucosides, acetophenone glucosides, glucopyranoside derivatives, genine derivatives, flavonol, anthocyanin and others.

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Research paper thumbnail of Screening of traditional antidiabetic medicinal plants of mauritius for possibleα-amylase inhibitory effectsin vitro

Phytotherapy …, 2006

In this study, seven exotic/indigenous medicinal plants of Mauritius, namely Coix lacryma-jobi (P... more In this study, seven exotic/indigenous medicinal plants of Mauritius, namely Coix lacryma-jobi (Poaceae), Aegle marmelos (Rutaceae), Artocarpus heterophyllus (Moraceae), Vangueria madagascariensis (Rubiaceae), Azadirachta indica (Meliaceae), Eriobotrya japonica (Rosaceae) and Syzigium cumini (Myrtaceae) were studied for possible effects on starch breakdown by α-amylase in vitro. The results showed that only Artocarpus heterophyllus significantly (p < 0.05) inhibited α-amylase activity in vitro. To confirm the observed effects, a further biochemical assay was undertaken to investigate the effects of Artocarpus heterophyllus on α-amylase activity using rat plasma in vitro. It was found that the aqueous leaf extract significantly (p < 0.05) inhibited α-amylase activity in rat plasma. The highest inhibitory activity (27.20 ± 5.00%) was observed at a concentration of 1000 µg/mL. However, in both cases dose dependency was not observed. Enzyme kinetic studies using the Michaelis-Menten and Lineweaver-Burk equations were performed to establish the type of inhibition involved. In the presence of the plant extract the maximal velocity (Vmax) remained constant (1/150 g / L/s) whereas the Michaelis-Menten constant (Km) increased by 5.79 g / L, indicating that the aqueous leaf extract of Artocarpus heterophyllus behaved as a competitive inhibitor. Results from the present study tend to indicate that Artocarpus heterophyllus could act as a ‘starch blocker’ thereby reducing post-prandial glucose peaks. Copyright © 2006 John Wiley & Sons, Ltd.

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Research paper thumbnail of Comparative chemical composition of leaves of some antidiabetic medicinal plants: Azadirachta indica, Vernonia amygdalina and Gongronema latifolium

African Journal of …, 2009

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Research paper thumbnail of Elemental Content of Some AntiDiabetic Medicinal Plants Using PIXE Analysis

Journal of Herbs, Spices & Medicinal Plants, 2010

Elemental analysis of some selected medicinal plants of northeast India having anti-diabetic prop... more Elemental analysis of some selected medicinal plants of northeast India having anti-diabetic property was conducted using proton-induced X-ray emission (PIXE) technique. Plant samples were excited with a 2.4-MeV proton beam from a 3-MV Tandetron accelerator, and the spectral data were analyzed by using GUPIX, a software package for fitting PIXE spectra from layered specimens. The elements K, Ca, Mn, Fe,

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Research paper thumbnail of Evaluation of α-amylase inhibitory activities of selected antidiabetic medicinal plants

Inhibitors of carbohydrate hydrolyzing enzymes such as α-amylase play an important role for the c... more Inhibitors of carbohydrate hydrolyzing enzymes such as α-amylase play an important role for the control of diabetes mellitus especially in patients with type 2 diabetes. In this study we selected ten antidiabetic medicinal plants, because they have been recommended to treat diabetes in traditional Iranian medicine, and screened them for α-amylase inhibitory activities. Among the tested samples, Camellia sinensis (Theaceae) leaf (IC50 = 1.54 mg/mL), Trigonella foenum-graecum (Leguminosae) seed (IC50 = 1.87 mg/mL) and leaf (IC50 = 1.92 mg/mL), and Urtica dioica (Urticaceae) leaf (IC50 = 1.89 mg/mL) revealed appreciable α-amylase inhibitory activities in a concentration-dependent manner. Furthermore, the most active sample, Camellia sinensis leaf, was partitioned by stepwise solvent–solvent extraction process and the inhibitory effect of each fraction on the α-amylase was tested. According to the results, the ethyl acetate fraction (IC50 = 0.53 mg/mL) and the residue (IC50 = 0.52 mg/mL) had the highest α-amylase inhibitory activities.

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Research paper thumbnail of Medicinal plants of India with anti-diabetic potential

Journal of ethnopharmacology, 2002

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Research paper thumbnail of Antidiabetic activity of medicinal plants and its relationship with their antioxidant property

Journal of ethnopharmacology, 2002

Methanolic extract (75%) of Terminalia chebula, Terminalia belerica, Emblica officinalis and thei... more Methanolic extract (75%) of Terminalia chebula, Terminalia belerica, Emblica officinalis and their combination named ‘Triphala’ (equal proportion of above three plant extracts) are being used extensively in Indian system of medicine. They were found to inhibit lipid peroxide formation and to scavenge hydroxyl and superoxide radicals in vitro. The concentration of plant extracts that inhibited 50% of lipid peroxidation induced with Fe2+/ascorbate were food to be 85.5, 27, 74 and 69 μg/ml, respectively. The concentration needed for the inhibition of hydoxyl radical scavenging were 165, 71, 155.5 and 151 μg/ml, and that for superoxide scavenging activity were found to be 20.5, 40.5, 6.5 and 12.5 μg/ml, respectively. Oral administration of the extracts (100 mg/kg body weight) reduced the blood sugar level in normal and in alloxan (120 mg/kg) diabetic rats significantly within 4 h. Continued, daily administration of the drug produced a sustained effect.

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Research paper thumbnail of Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay

Bookmarks Related papers MentionsView impact

Research paper thumbnail of An overview on antidiabetic medicinal plants having insulin mimetic property

Asian Pacific Journal of …, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Aldose reductase inhibitory activity and anti catraract potential of some traditionally acclaimed antidiabetic medicinal plants

Oriental Pharmacy and Experimental Medicine, 2009

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Antidiabetic Medicinal Plants as a Source of Alpha Glucosidase Inhibitors

Current diabetes …, 2010

The aim of this study is to collate all available data on antidiabetic plants that inhibit alpha ... more The aim of this study is to collate all available data on antidiabetic plants that inhibit alpha glucosidase, reported mainly by Medline (PubMed) these last years. In the present study, interest is focused on experimental researches conducted on hypoglycemic plants particularly those which show alpha glucosidase inhibitor activity alongside bioactive components. This study describes 47 species that belong to 29 families. The plant families, which enclose the species, studied most as inhibitors of alphaglucosidase, are Fabaceae (6 species.), Crassulaceae (3 species), Hippocrateacaea (3 species), Lamiaceae (3 species), and Myrtaceae (3 species), with most studied species being Salacia reticulata (Hippocrateaceae) and Morus alba (Moraceae). The study also covers natural products (active natural components and crude extracts) isolated from the medicinal plants which inhibit alpha glucosidase as reported this last decade. Many kinds of these isolated natural products show strong activity such as, Alkaloids, stilbenoids (polyphenol), triterpene, acids (chlorogenic acid, betulinic acid, syringic acid, vanillic acid, bartogenic acid, oleanolic acid, dehydrotrametenolic acid, corosolic acid, ellagic acid, ursolic acid, gallic acid), phytosterol, myoinositol, flavonoids, Flavonolignans, anthraquinones, anthrones, and xanthones, Feruloylglucosides, flavanone glucosides, acetophenone glucosides, glucopyranoside derivatives, genine derivatives, flavonol, anthocyanin and others.

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Research paper thumbnail of Screening of traditional antidiabetic medicinal plants of mauritius for possibleα-amylase inhibitory effectsin vitro

Phytotherapy …, 2006

In this study, seven exotic/indigenous medicinal plants of Mauritius, namely Coix lacryma-jobi (P... more In this study, seven exotic/indigenous medicinal plants of Mauritius, namely Coix lacryma-jobi (Poaceae), Aegle marmelos (Rutaceae), Artocarpus heterophyllus (Moraceae), Vangueria madagascariensis (Rubiaceae), Azadirachta indica (Meliaceae), Eriobotrya japonica (Rosaceae) and Syzigium cumini (Myrtaceae) were studied for possible effects on starch breakdown by α-amylase in vitro. The results showed that only Artocarpus heterophyllus significantly (p < 0.05) inhibited α-amylase activity in vitro. To confirm the observed effects, a further biochemical assay was undertaken to investigate the effects of Artocarpus heterophyllus on α-amylase activity using rat plasma in vitro. It was found that the aqueous leaf extract significantly (p < 0.05) inhibited α-amylase activity in rat plasma. The highest inhibitory activity (27.20 ± 5.00%) was observed at a concentration of 1000 µg/mL. However, in both cases dose dependency was not observed. Enzyme kinetic studies using the Michaelis-Menten and Lineweaver-Burk equations were performed to establish the type of inhibition involved. In the presence of the plant extract the maximal velocity (Vmax) remained constant (1/150 g / L/s) whereas the Michaelis-Menten constant (Km) increased by 5.79 g / L, indicating that the aqueous leaf extract of Artocarpus heterophyllus behaved as a competitive inhibitor. Results from the present study tend to indicate that Artocarpus heterophyllus could act as a ‘starch blocker’ thereby reducing post-prandial glucose peaks. Copyright © 2006 John Wiley & Sons, Ltd.

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Research paper thumbnail of Comparative chemical composition of leaves of some antidiabetic medicinal plants: Azadirachta indica, Vernonia amygdalina and Gongronema latifolium

African Journal of …, 2009

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Research paper thumbnail of Elemental Content of Some AntiDiabetic Medicinal Plants Using PIXE Analysis

Journal of Herbs, Spices & Medicinal Plants, 2010

Elemental analysis of some selected medicinal plants of northeast India having anti-diabetic prop... more Elemental analysis of some selected medicinal plants of northeast India having anti-diabetic property was conducted using proton-induced X-ray emission (PIXE) technique. Plant samples were excited with a 2.4-MeV proton beam from a 3-MV Tandetron accelerator, and the spectral data were analyzed by using GUPIX, a software package for fitting PIXE spectra from layered specimens. The elements K, Ca, Mn, Fe,

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Evaluation of α-amylase inhibitory activities of selected antidiabetic medicinal plants

Inhibitors of carbohydrate hydrolyzing enzymes such as α-amylase play an important role for the c... more Inhibitors of carbohydrate hydrolyzing enzymes such as α-amylase play an important role for the control of diabetes mellitus especially in patients with type 2 diabetes. In this study we selected ten antidiabetic medicinal plants, because they have been recommended to treat diabetes in traditional Iranian medicine, and screened them for α-amylase inhibitory activities. Among the tested samples, Camellia sinensis (Theaceae) leaf (IC50 = 1.54 mg/mL), Trigonella foenum-graecum (Leguminosae) seed (IC50 = 1.87 mg/mL) and leaf (IC50 = 1.92 mg/mL), and Urtica dioica (Urticaceae) leaf (IC50 = 1.89 mg/mL) revealed appreciable α-amylase inhibitory activities in a concentration-dependent manner. Furthermore, the most active sample, Camellia sinensis leaf, was partitioned by stepwise solvent–solvent extraction process and the inhibitory effect of each fraction on the α-amylase was tested. According to the results, the ethyl acetate fraction (IC50 = 0.53 mg/mL) and the residue (IC50 = 0.52 mg/mL) had the highest α-amylase inhibitory activities.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Medicinal plants of India with anti-diabetic potential

Journal of ethnopharmacology, 2002

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Antidiabetic activity of medicinal plants and its relationship with their antioxidant property

Journal of ethnopharmacology, 2002

Methanolic extract (75%) of Terminalia chebula, Terminalia belerica, Emblica officinalis and thei... more Methanolic extract (75%) of Terminalia chebula, Terminalia belerica, Emblica officinalis and their combination named ‘Triphala’ (equal proportion of above three plant extracts) are being used extensively in Indian system of medicine. They were found to inhibit lipid peroxide formation and to scavenge hydroxyl and superoxide radicals in vitro. The concentration of plant extracts that inhibited 50% of lipid peroxidation induced with Fe2+/ascorbate were food to be 85.5, 27, 74 and 69 μg/ml, respectively. The concentration needed for the inhibition of hydoxyl radical scavenging were 165, 71, 155.5 and 151 μg/ml, and that for superoxide scavenging activity were found to be 20.5, 40.5, 6.5 and 12.5 μg/ml, respectively. Oral administration of the extracts (100 mg/kg body weight) reduced the blood sugar level in normal and in alloxan (120 mg/kg) diabetic rats significantly within 4 h. Continued, daily administration of the drug produced a sustained effect.

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