Robin-van-den Berg - Academia.edu (original) (raw)

Papers by Robin-van-den Berg

Research paper thumbnail of Analytical, Nutritional and Clinical Methods Section Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures

a-Pinene and b-myrcene are compounds that are contained in mastic gum in high concentrations.The ... more a-Pinene and b-myrcene are compounds that are contained in mastic gum in high concentrations.The b-myrcene percentage determines the marketability of mastic gums.The chemical composition of mastic gum oil of a representative resin quality was evaluated by gas chromatography-mass spectrometry (GC-MS) technique.FT-Raman spectroscopy, based on band intensity measurements, was used for the determination of a-pinene and b-myrcene content in mastic gum.Bands at 1658 and 1633 cm � 1 were used for the calibration of a-pinene and b-myrcene, respectively.Calibration curves were linear (correlation coefficient for a- pinene was 0.992 and 0.997 for b-myrcene) in the range 30-80 and 3-45%, respectively.Normalization of calibration curves, against the 802 cm� 1 cyclohexane band, minimized the effect of laser beam power fluctuations.The proposed method is rapid and simple. Accordingly, mastic gum oils from Chios island (Greece) contained 38.1-69.5% a-pinene and 4.5-57.9% b-myrcene. # 2002 Elsevier Science Ltd.All rights reserved.

Research paper thumbnail of (closed circles) Pine nut FFA, (closed triangles) pine nut TG, (open squares) placebo

Significant differences (p < 0.05) indicated by a: pine nut FFA versus placebo, b: pine nut TG... more Significant differences (p < 0.05) indicated by a: pine nut FFA versus placebo, b: pine nut TG versus placebo.<b>Copyright information:</b>Taken from "The effect of Korean pine nut oil on in vitro CCK release, on appetite sensations and on gut hormones in post-menopausal overweight women"http://www.lipidworld.com/content/7/1/10Lipids in Health and Disease 2008;7():10-10.Published online 20 Mar 2008PMCID:PMC2322999.

Research paper thumbnail of Korean pine nut fatty acids affect appetite sensations, plasma CCK and GLP1 in overweight subjects

Research paper thumbnail of Nuclear factor-��B activation is higher in peripheral blood mononuclear cells of male smokers

Research paper thumbnail of Pinnothin™ suppresses appetite in overweight women

Research paper thumbnail of Mayonnaise Increases the Relative Bioavailability of Fat-Soluble (Non)-Nutrients from a Salad

International Journal of Nutrition and Food Sciences, 2015

Vegetables are good sources of vitamins. For that reason the World Health Organisation recommends... more Vegetables are good sources of vitamins. For that reason the World Health Organisation recommends daily consumption of at least 200g of vegetables. However, this requirement is met only partially worldwide. Increasing the bioavailability of fat-soluble (non)-nutrients could make intake of those sources more effective. Here, the hypothesis was tested whether this goal can be achieved by adding mayonnaise to a dressing for a salad. Nine healthy subjects (28.7±4.7 years old, BMI of 26.7±2.1 kg/m 2) consumed a salad either with a basic dressing (control) or a basic dressing with mayonnaise (test). After both interventions, α-carotene, β-carotene, vitamin K 1 and retinyl palmitate were measured in chylomicron-rich plasma fractions. The average relative bioavailability (-95% CI, +95% CI) was +80% (-3%, 232%) for α-carotene (P=0.0591), +91% (22%, 200%) for β-carotene (P=0.0118), and +116% (5%, 344%) for vitamin K 1 (P=0.0392). Retinyl palmitate AUC's increased by 117% (39%, 242%) (P=0.0039), hence bioconversion of pro-vitamin A to retinol doubled. In conclusion, addition of mayonnaise to a dressing for a salad increased the uptake of the (non)-nutrients analysed in this study. Therefore, adding mayonnaise may be an interesting dietary approach to improve the nutritive value of vegetables, which are frequently underconsumed.

Research paper thumbnail of Bioprocessing of wheat bran improves in vitro bioaccessibility and colonic metabolism of phenolic compounds

Ferulic acid (FA) is the most abundant phenolic compound in wheat grain, mainly located in the br... more Ferulic acid (FA) is the most abundant phenolic compound in wheat grain, mainly located in the bran. However, its bioaccessibility from the bran matrix is extremely low. Different bioprocessing techniques involving fermentation or enzymatic and fermentation treatments of wheat bran were developed aiming at improving the bioaccessibility of phenolic compounds in bran-containing breads. The bioaccessibility of ferulic acid, p-coumaric acid, and sinapic acid was assessed with an in vitro model of upper gastrointestinal tract (TIM-1). Colonic metabolism of the phenolic compounds in the nonbioaccessible fraction of the breads was studied with an in vitro model of human colon (TIM-2). The most effective treatment was the combination of enzymes and fermentation that increased the bioaccessibility of FA from 1.1% to 5.5%. The major colonic metabolites were 3-(3-hydroxyphenyl)propionic acid and 3-phenylpropionic acid. Bran bioprocessing increases the bioaccessibility of phenolic compounds as well as the colonic end metabolite 3-phenylpropionic acid.

Research paper thumbnail of Transcription Factor NF-kB as a Potential Biomarker of Oxidative Stress to Evaluate Antioxidant Effects

Research paper thumbnail of Direct analysis of aldehydes and carboxylic acids in the gas phase by negative ionization SIFT-MS: quantification and modeling of ion-molecule reactions

Rapid Communications in Mass Spectrometry

Research paper thumbnail of Sodium pyrophosphate enhances iron bioavailability from bouillon cubes fortified with ferric pyrophosphate

The British journal of nutrition, Jan 8, 2016

Fe fortification of centrally manufactured and frequently consumed condiments such as bouillon cu... more Fe fortification of centrally manufactured and frequently consumed condiments such as bouillon cubes could help prevent Fe deficiency in developing countries. However, Fe compounds that do not cause sensory changes in the fortified product, such as ferric pyrophosphate (FePP), exhibit low absorption in humans. Tetra sodium pyrophosphate (NaPP) can form soluble complexes with Fe, which could increase Fe bioavailability. Therefore, the aim of this study was to investigate Fe bioavailability from bouillon cubes fortified with either FePP only, FePP+NaPP, ferrous sulphate (FeSO4) only, or FeSO4+NaPP. We first conducted in vitro studies using a protocol of simulated digestion to assess the dialysable and ionic Fe, and the cellular ferritin response in a Caco-2 cell model. Second, Fe absorption from bouillon prepared from intrinsically labelled cubes (2·5 mg stable Fe isotopes/cube) was assessed in twenty-four Fe-deficient women, by measuring Fe incorporation into erythrocytes 2 weeks aft...

Research paper thumbnail of Effect of Harvesting, Processing and Preparation on the Nutrient Retention of (Phyto) Nutrients of Dried and Homemade Soups

Free Radical Biology and Medicine, 2013

Research paper thumbnail of Effect of Harvesting, Processing and Preparation on the Nutrient Retention of (Phyto) Nutrients of Dried and Homemade Soups

Research paper thumbnail of A Vegetable/Fruit Concentrate with High Antioxidant Capacity Has No Effect on Biomarkers of Antioxidant Status in Male Smokers

The Journal of Nutrition, Jun 1, 2001

The potential benefits of a high fruit and vegetable intake on the antioxidant status and on rele... more The potential benefits of a high fruit and vegetable intake on the antioxidant status and on relevant biomarkers of oxidative damage to lipids, proteins and DNA and on (functional) markers of oxidative stress were evaluated. A randomized, free living, open placebo-controlled cross-over trial of 3 wk, with a 2-wk washout period between treatments, was performed in a group of 22 male smokers with a relatively low vegetable and fruit intake using a vegetable burger and fruit drink. The vegetable burger and fruit drink increased serum levels of vitamin C, ␣-carotene, ␤-carotene, ␤-cryptoxanthin and zeaxanthin and plasma total antioxidant capacity. However, no effects were demonstrated on any marker of oxidative damage to lipids (malondialdehyde F 2 -isoprostane) proteins (carbonyls) and DNA (Comet assay) and (functional) markers of oxidative stress (reduced/oxidized glutathione ratio, glutathione-S-transferase ␣, glutathione-S-transferase and nuclear transcription factor-B). Apparently, these increased levels of antioxidants in serum were not sufficiently high to show beneficial changes with the selected biomarkers. Alternatively, oxidative stress in male smokers with a relatively low fruit and vegetable intake might have been still too low to demonstrate a beneficial effect of antioxidants.

Research paper thumbnail of Transcription factor NF-?B as a potential biomarker for oxidative stress

Research paper thumbnail of Biomarkers for Evaluating Antioxidant Effects

Oxidative Stress and Disease, 2005

Research paper thumbnail of Co-ingestion of a protein hydrolysate with or without additional leucine effectively reduces postprandial blood glucose excursions in Type 2 diabetic men

The Journal of nutrition, 2006

This study examined postprandial plasma insulin and glucose responses after co-ingestion of an in... more This study examined postprandial plasma insulin and glucose responses after co-ingestion of an insulinotropic protein (Pro) hydrolysate with and without additional free leucine with a single bolus of carbohydrate (Cho). Male patients with long-standing Type 2 diabetes (n = 10) and healthy controls (n = 10) participated in 3 trials in which plasma glucose, insulin, and amino acid responses were determined after the ingestion of beverages of different composition (Cho: 0.7 g/kg carbohydrate, Cho+Pro: 0.7 g/kg carbohydrate with 0.3 g/kg protein hydrolysate, or Cho+Pro+Leu: 0.7 g/kg carbohydrate, 0.3 g/kg protein hydrolysate and 0.1 g/kg free leucine). Plasma insulin responses [expressed as area under the curve (AUC)] were 141 and 204% greater in patients with Type 2 diabetes and 66 and 221% greater in the controls in the Cho+Pro and Cho+Pro+Leu trials, respectively, compared with those in the Cho trial (P < 0.05). The concomitant plasma glucose responses were 15 and 12% lower in the...

Research paper thumbnail of A vegetable/fruit concentrate with high antioxidant capacity has no effect on biomarkers of antioxidant status in male smokers

The Journal of nutrition, 2001

The potential benefits of a high fruit and vegetable intake on the antioxidant status and on rele... more The potential benefits of a high fruit and vegetable intake on the antioxidant status and on relevant biomarkers of oxidative damage to lipids, proteins and DNA and on (functional) markers of oxidative stress were evaluated. A randomized, free living, open placebo-controlled cross-over trial of 3 wk, with a 2-wk washout period between treatments, was performed in a group of 22 male smokers with a relatively low vegetable and fruit intake using a vegetable burger and fruit drink. The vegetable burger and fruit drink increased serum levels of vitamin C, alpha-carotene, beta-carotene, beta-cryptoxanthin and zeaxanthin and plasma total antioxidant capacity. However, no effects were demonstrated on any marker of oxidative damage to lipids (malondialdehyde F(2)-isoprostane) proteins (carbonyls) and DNA (Comet assay) and (functional) markers of oxidative stress (reduced/oxidized glutathione ratio, glutathione-S-transferase alpha, glutathione-S-transferase pi and nuclear transcription facto...

Research paper thumbnail of Bioavailability of ferulic acid is determined by its bioaccessibility

Journal of Cereal Science, 2009

Epidemiological studies have linked whole grain consumption to prevention of several chronic dise... more Epidemiological studies have linked whole grain consumption to prevention of several chronic diseases. Whole grain is a source of important phytochemicals, such as ferulic acid (FA). FA is the most abundant phenolic and major contributor to the in vitro antioxidant capacity of wheat grain. Several studies have reported highly variable results on FA bioavailability (0.4–98%). The binding of FA to

Research paper thumbnail of Ferulic Acid from Aleurone Determines the Antioxidant Potency of Wheat Grain ( Triticum aestivum L.)

Journal of Agricultural and Food Chemistry, 2008

Grain is an important source of phytochemicals which have potent antioxidant capacity. They have ... more Grain is an important source of phytochemicals which have potent antioxidant capacity. They have been implicated in the beneficial health effect of whole grains in reducing cardiovascular disease and type 2 diabetes. The aim of the present study was to identify the most important antioxidant fractions of wheat grain. It was found that the aleurone content of these fractions was highly correlated with the antioxidant capacity of the fractions (r = 0.96, p <0.0001). Ferulic acid appeared to be the major contributor to the antioxidant capacity in the fractions with higher antioxidant capacity. The contribution of protein, was rather limited. It was concluded that the antioxidant potency of wheat grain fractions is predominantly determined by the aleurone content, which can be attributed to the presence of relatively large amount of phenolic compounds, primarily ferulic acid.

Research paper thumbnail of The predictive value of the antioxidant capacity of structurally related flavonoids using the Trolox equivalent antioxidant capacity (TEAC) assay

Food Chemistry, 2000

The antioxidant capacity of a series of structurally related¯avonoids is quanti®ed by the amount ... more The antioxidant capacity of a series of structurally related¯avonoids is quanti®ed by the amount of ABTS [2,2 H -azinobis-(3ethylbenzthiazoline-6-sulfonate)] radical anions (ABTS À ) that is able to react with the¯avonoid and expressed as the Trolox equivalent antioxidant capacity (TEAC). To evaluate the predictive value of the TEAC, the antioxidant activity of this series of avonoids was also assessed in other in vitro assays, measuring the eect on hydroxyl scavenging, lipid peroxidation and doxorubicin-induced toxicity as typical scavenging or damage assays. The¯avonoids tested were mono HER, di HER, tri HER, tetra HER and tri HEQ, diering in the number of aromatic hydroxyl groups. It was found that these compounds showed both a fast and slow scavenging eect in the TEAC assay and therefore the TEAC at 10 s (`fast' TEAC) and 6 min (`total' TEAC) was determined. Both this`total' and`fast' TEAC are negatively correlated with hydroxyl radical scavenging. The`total' TEAC showed a better correlation than the`fast' TEAC with the inhibition of lipid peroxidation and the protection against doxorubicin-induced toxicity. This indicates that beside the fast reaction of scavengers with the ABTS radical, also the slow reaction should be taken into consideration. It is concluded that the antioxidant capacity, assessed with the modi®ed TEAC assay, can be useful to predict the in vivo antioxidant eect in a series of structurally related compounds. #

Research paper thumbnail of Analytical, Nutritional and Clinical Methods Section Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures

a-Pinene and b-myrcene are compounds that are contained in mastic gum in high concentrations.The ... more a-Pinene and b-myrcene are compounds that are contained in mastic gum in high concentrations.The b-myrcene percentage determines the marketability of mastic gums.The chemical composition of mastic gum oil of a representative resin quality was evaluated by gas chromatography-mass spectrometry (GC-MS) technique.FT-Raman spectroscopy, based on band intensity measurements, was used for the determination of a-pinene and b-myrcene content in mastic gum.Bands at 1658 and 1633 cm � 1 were used for the calibration of a-pinene and b-myrcene, respectively.Calibration curves were linear (correlation coefficient for a- pinene was 0.992 and 0.997 for b-myrcene) in the range 30-80 and 3-45%, respectively.Normalization of calibration curves, against the 802 cm� 1 cyclohexane band, minimized the effect of laser beam power fluctuations.The proposed method is rapid and simple. Accordingly, mastic gum oils from Chios island (Greece) contained 38.1-69.5% a-pinene and 4.5-57.9% b-myrcene. # 2002 Elsevier Science Ltd.All rights reserved.

Research paper thumbnail of (closed circles) Pine nut FFA, (closed triangles) pine nut TG, (open squares) placebo

Significant differences (p < 0.05) indicated by a: pine nut FFA versus placebo, b: pine nut TG... more Significant differences (p < 0.05) indicated by a: pine nut FFA versus placebo, b: pine nut TG versus placebo.<b>Copyright information:</b>Taken from "The effect of Korean pine nut oil on in vitro CCK release, on appetite sensations and on gut hormones in post-menopausal overweight women"http://www.lipidworld.com/content/7/1/10Lipids in Health and Disease 2008;7():10-10.Published online 20 Mar 2008PMCID:PMC2322999.

Research paper thumbnail of Korean pine nut fatty acids affect appetite sensations, plasma CCK and GLP1 in overweight subjects

Research paper thumbnail of Nuclear factor-��B activation is higher in peripheral blood mononuclear cells of male smokers

Research paper thumbnail of Pinnothin™ suppresses appetite in overweight women

Research paper thumbnail of Mayonnaise Increases the Relative Bioavailability of Fat-Soluble (Non)-Nutrients from a Salad

International Journal of Nutrition and Food Sciences, 2015

Vegetables are good sources of vitamins. For that reason the World Health Organisation recommends... more Vegetables are good sources of vitamins. For that reason the World Health Organisation recommends daily consumption of at least 200g of vegetables. However, this requirement is met only partially worldwide. Increasing the bioavailability of fat-soluble (non)-nutrients could make intake of those sources more effective. Here, the hypothesis was tested whether this goal can be achieved by adding mayonnaise to a dressing for a salad. Nine healthy subjects (28.7±4.7 years old, BMI of 26.7±2.1 kg/m 2) consumed a salad either with a basic dressing (control) or a basic dressing with mayonnaise (test). After both interventions, α-carotene, β-carotene, vitamin K 1 and retinyl palmitate were measured in chylomicron-rich plasma fractions. The average relative bioavailability (-95% CI, +95% CI) was +80% (-3%, 232%) for α-carotene (P=0.0591), +91% (22%, 200%) for β-carotene (P=0.0118), and +116% (5%, 344%) for vitamin K 1 (P=0.0392). Retinyl palmitate AUC's increased by 117% (39%, 242%) (P=0.0039), hence bioconversion of pro-vitamin A to retinol doubled. In conclusion, addition of mayonnaise to a dressing for a salad increased the uptake of the (non)-nutrients analysed in this study. Therefore, adding mayonnaise may be an interesting dietary approach to improve the nutritive value of vegetables, which are frequently underconsumed.

Research paper thumbnail of Bioprocessing of wheat bran improves in vitro bioaccessibility and colonic metabolism of phenolic compounds

Ferulic acid (FA) is the most abundant phenolic compound in wheat grain, mainly located in the br... more Ferulic acid (FA) is the most abundant phenolic compound in wheat grain, mainly located in the bran. However, its bioaccessibility from the bran matrix is extremely low. Different bioprocessing techniques involving fermentation or enzymatic and fermentation treatments of wheat bran were developed aiming at improving the bioaccessibility of phenolic compounds in bran-containing breads. The bioaccessibility of ferulic acid, p-coumaric acid, and sinapic acid was assessed with an in vitro model of upper gastrointestinal tract (TIM-1). Colonic metabolism of the phenolic compounds in the nonbioaccessible fraction of the breads was studied with an in vitro model of human colon (TIM-2). The most effective treatment was the combination of enzymes and fermentation that increased the bioaccessibility of FA from 1.1% to 5.5%. The major colonic metabolites were 3-(3-hydroxyphenyl)propionic acid and 3-phenylpropionic acid. Bran bioprocessing increases the bioaccessibility of phenolic compounds as well as the colonic end metabolite 3-phenylpropionic acid.

Research paper thumbnail of Transcription Factor NF-kB as a Potential Biomarker of Oxidative Stress to Evaluate Antioxidant Effects

Research paper thumbnail of Direct analysis of aldehydes and carboxylic acids in the gas phase by negative ionization SIFT-MS: quantification and modeling of ion-molecule reactions

Rapid Communications in Mass Spectrometry

Research paper thumbnail of Sodium pyrophosphate enhances iron bioavailability from bouillon cubes fortified with ferric pyrophosphate

The British journal of nutrition, Jan 8, 2016

Fe fortification of centrally manufactured and frequently consumed condiments such as bouillon cu... more Fe fortification of centrally manufactured and frequently consumed condiments such as bouillon cubes could help prevent Fe deficiency in developing countries. However, Fe compounds that do not cause sensory changes in the fortified product, such as ferric pyrophosphate (FePP), exhibit low absorption in humans. Tetra sodium pyrophosphate (NaPP) can form soluble complexes with Fe, which could increase Fe bioavailability. Therefore, the aim of this study was to investigate Fe bioavailability from bouillon cubes fortified with either FePP only, FePP+NaPP, ferrous sulphate (FeSO4) only, or FeSO4+NaPP. We first conducted in vitro studies using a protocol of simulated digestion to assess the dialysable and ionic Fe, and the cellular ferritin response in a Caco-2 cell model. Second, Fe absorption from bouillon prepared from intrinsically labelled cubes (2·5 mg stable Fe isotopes/cube) was assessed in twenty-four Fe-deficient women, by measuring Fe incorporation into erythrocytes 2 weeks aft...

Research paper thumbnail of Effect of Harvesting, Processing and Preparation on the Nutrient Retention of (Phyto) Nutrients of Dried and Homemade Soups

Free Radical Biology and Medicine, 2013

Research paper thumbnail of Effect of Harvesting, Processing and Preparation on the Nutrient Retention of (Phyto) Nutrients of Dried and Homemade Soups

Research paper thumbnail of A Vegetable/Fruit Concentrate with High Antioxidant Capacity Has No Effect on Biomarkers of Antioxidant Status in Male Smokers

The Journal of Nutrition, Jun 1, 2001

The potential benefits of a high fruit and vegetable intake on the antioxidant status and on rele... more The potential benefits of a high fruit and vegetable intake on the antioxidant status and on relevant biomarkers of oxidative damage to lipids, proteins and DNA and on (functional) markers of oxidative stress were evaluated. A randomized, free living, open placebo-controlled cross-over trial of 3 wk, with a 2-wk washout period between treatments, was performed in a group of 22 male smokers with a relatively low vegetable and fruit intake using a vegetable burger and fruit drink. The vegetable burger and fruit drink increased serum levels of vitamin C, ␣-carotene, ␤-carotene, ␤-cryptoxanthin and zeaxanthin and plasma total antioxidant capacity. However, no effects were demonstrated on any marker of oxidative damage to lipids (malondialdehyde F 2 -isoprostane) proteins (carbonyls) and DNA (Comet assay) and (functional) markers of oxidative stress (reduced/oxidized glutathione ratio, glutathione-S-transferase ␣, glutathione-S-transferase and nuclear transcription factor-B). Apparently, these increased levels of antioxidants in serum were not sufficiently high to show beneficial changes with the selected biomarkers. Alternatively, oxidative stress in male smokers with a relatively low fruit and vegetable intake might have been still too low to demonstrate a beneficial effect of antioxidants.

Research paper thumbnail of Transcription factor NF-?B as a potential biomarker for oxidative stress

Research paper thumbnail of Biomarkers for Evaluating Antioxidant Effects

Oxidative Stress and Disease, 2005

Research paper thumbnail of Co-ingestion of a protein hydrolysate with or without additional leucine effectively reduces postprandial blood glucose excursions in Type 2 diabetic men

The Journal of nutrition, 2006

This study examined postprandial plasma insulin and glucose responses after co-ingestion of an in... more This study examined postprandial plasma insulin and glucose responses after co-ingestion of an insulinotropic protein (Pro) hydrolysate with and without additional free leucine with a single bolus of carbohydrate (Cho). Male patients with long-standing Type 2 diabetes (n = 10) and healthy controls (n = 10) participated in 3 trials in which plasma glucose, insulin, and amino acid responses were determined after the ingestion of beverages of different composition (Cho: 0.7 g/kg carbohydrate, Cho+Pro: 0.7 g/kg carbohydrate with 0.3 g/kg protein hydrolysate, or Cho+Pro+Leu: 0.7 g/kg carbohydrate, 0.3 g/kg protein hydrolysate and 0.1 g/kg free leucine). Plasma insulin responses [expressed as area under the curve (AUC)] were 141 and 204% greater in patients with Type 2 diabetes and 66 and 221% greater in the controls in the Cho+Pro and Cho+Pro+Leu trials, respectively, compared with those in the Cho trial (P < 0.05). The concomitant plasma glucose responses were 15 and 12% lower in the...

Research paper thumbnail of A vegetable/fruit concentrate with high antioxidant capacity has no effect on biomarkers of antioxidant status in male smokers

The Journal of nutrition, 2001

The potential benefits of a high fruit and vegetable intake on the antioxidant status and on rele... more The potential benefits of a high fruit and vegetable intake on the antioxidant status and on relevant biomarkers of oxidative damage to lipids, proteins and DNA and on (functional) markers of oxidative stress were evaluated. A randomized, free living, open placebo-controlled cross-over trial of 3 wk, with a 2-wk washout period between treatments, was performed in a group of 22 male smokers with a relatively low vegetable and fruit intake using a vegetable burger and fruit drink. The vegetable burger and fruit drink increased serum levels of vitamin C, alpha-carotene, beta-carotene, beta-cryptoxanthin and zeaxanthin and plasma total antioxidant capacity. However, no effects were demonstrated on any marker of oxidative damage to lipids (malondialdehyde F(2)-isoprostane) proteins (carbonyls) and DNA (Comet assay) and (functional) markers of oxidative stress (reduced/oxidized glutathione ratio, glutathione-S-transferase alpha, glutathione-S-transferase pi and nuclear transcription facto...

Research paper thumbnail of Bioavailability of ferulic acid is determined by its bioaccessibility

Journal of Cereal Science, 2009

Epidemiological studies have linked whole grain consumption to prevention of several chronic dise... more Epidemiological studies have linked whole grain consumption to prevention of several chronic diseases. Whole grain is a source of important phytochemicals, such as ferulic acid (FA). FA is the most abundant phenolic and major contributor to the in vitro antioxidant capacity of wheat grain. Several studies have reported highly variable results on FA bioavailability (0.4–98%). The binding of FA to

Research paper thumbnail of Ferulic Acid from Aleurone Determines the Antioxidant Potency of Wheat Grain ( Triticum aestivum L.)

Journal of Agricultural and Food Chemistry, 2008

Grain is an important source of phytochemicals which have potent antioxidant capacity. They have ... more Grain is an important source of phytochemicals which have potent antioxidant capacity. They have been implicated in the beneficial health effect of whole grains in reducing cardiovascular disease and type 2 diabetes. The aim of the present study was to identify the most important antioxidant fractions of wheat grain. It was found that the aleurone content of these fractions was highly correlated with the antioxidant capacity of the fractions (r = 0.96, p <0.0001). Ferulic acid appeared to be the major contributor to the antioxidant capacity in the fractions with higher antioxidant capacity. The contribution of protein, was rather limited. It was concluded that the antioxidant potency of wheat grain fractions is predominantly determined by the aleurone content, which can be attributed to the presence of relatively large amount of phenolic compounds, primarily ferulic acid.

Research paper thumbnail of The predictive value of the antioxidant capacity of structurally related flavonoids using the Trolox equivalent antioxidant capacity (TEAC) assay

Food Chemistry, 2000

The antioxidant capacity of a series of structurally related¯avonoids is quanti®ed by the amount ... more The antioxidant capacity of a series of structurally related¯avonoids is quanti®ed by the amount of ABTS [2,2 H -azinobis-(3ethylbenzthiazoline-6-sulfonate)] radical anions (ABTS À ) that is able to react with the¯avonoid and expressed as the Trolox equivalent antioxidant capacity (TEAC). To evaluate the predictive value of the TEAC, the antioxidant activity of this series of avonoids was also assessed in other in vitro assays, measuring the eect on hydroxyl scavenging, lipid peroxidation and doxorubicin-induced toxicity as typical scavenging or damage assays. The¯avonoids tested were mono HER, di HER, tri HER, tetra HER and tri HEQ, diering in the number of aromatic hydroxyl groups. It was found that these compounds showed both a fast and slow scavenging eect in the TEAC assay and therefore the TEAC at 10 s (`fast' TEAC) and 6 min (`total' TEAC) was determined. Both this`total' and`fast' TEAC are negatively correlated with hydroxyl radical scavenging. The`total' TEAC showed a better correlation than the`fast' TEAC with the inhibition of lipid peroxidation and the protection against doxorubicin-induced toxicity. This indicates that beside the fast reaction of scavengers with the ABTS radical, also the slow reaction should be taken into consideration. It is concluded that the antioxidant capacity, assessed with the modi®ed TEAC assay, can be useful to predict the in vivo antioxidant eect in a series of structurally related compounds. #