Trish Berger - Academia.edu (original) (raw)

Papers by Trish Berger

Research paper thumbnail of Gene Expression in Porcine Bulbourethral Glands

Research paper thumbnail of Gender disparity in survival of early porcine fetuses due to altered androgen receptor or associated U2 spliceosome component

Scientific Reports, Sep 11, 2023

Research paper thumbnail of Are testicular cortisol and WISP2 involved in estrogen-regulated Sertoli cell proliferation?

Animal Reproduction Science, Aug 1, 2019

Research paper thumbnail of Multiple Forms of Aromatase in Suiformes: Tissue Distribution, Regulation, and Functional Significance

Oxford University Press eBooks, Oct 19, 2012

Research paper thumbnail of Trichloroethylene metabolism in the rat ovary reduces oocyte fertilizability

Chemico-Biological Interactions, Oct 1, 2007

Research paper thumbnail of In vitro fertilization after in vivo treatment of rats with three reproductive toxicants☆

Reproductive Toxicology, 2000

Research paper thumbnail of In vivo exposure of female rats to toxicants may affect oocyte quality

Reproductive Toxicology, Jun 1, 2003

Research paper thumbnail of Effect of Male Accessory Gland Secretions on Sensitivity of Porcine Sperm Acrosomes to Cold Shock, Initiation of Motility and Loss of Cytoplasmic Droplets

Journal of Animal Science, May 1, 1985

Research paper thumbnail of Adsorption of seminal plasma proteins by boar spermatozoa

Theriogenology, Oct 1, 1990

Research paper thumbnail of The monomeric G-protein RhoA is widely expressed in mammalian sperm, but does not play a role in actin cytoskeletal reorganization in porcine sperm

Research paper thumbnail of Is there a sire-dam interaction in sperm fertilizing potential?

California Agriculture, Nov 1, 2001

Research paper thumbnail of Inhibition of Sperm Motility by Bovine Serum Components1

Biology of Reproduction, Mar 1, 1990

Research paper thumbnail of Potential role of alphav and beta1 integrins as oocyte adhesion molecules during fertilization in pigs

Reproduction, Sep 1, 2000

Research paper thumbnail of Comparison of techniques for selection of motile spermatozoa

Fertility and Sterility, Feb 1, 1985

Procedures to separate motile sperm with high rates of recovery may have clinical application in ... more Procedures to separate motile sperm with high rates of recovery may have clinical application in in vitro fertilization and intrauterine insemination in increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. A two-step continuous Percoll gradient was an effective means of separating motile sperm which also had enhanced ability to penetrate zona-free hamster ova. However, the requirement for a high-speed centrifuge and rotor makes the procedure impractical in many cases. A one-step discontinuous Percoll gradient was also effective in separating a population of motile sperm. Comparison of the discontinuous Percoll gradient with other techniques for separation of motile sperm indicated the discontinuous Percoll gradient had advantages in terms of recovery, enhancement of motility, and increased ability to penetrate zona-free hamster ova. The velocity of selected sperm was not significantly different among techniques. The one-step discontinuous Percoll gradient appears to have value both for increasing homogeneity of human sperm populations used for basic research and in clinical practice for male subfertility.

Research paper thumbnail of Factors Affecting Human Sperm Penetration of Zona-Free Hamster Ova

The Journal of Urology, Nov 1, 1983

Research paper thumbnail of Boar sperm plasma membrane protein profile: Correlation with the zona-free hamster ova bioassay

Theriogenology, 1994

densitometry. Variation in the quantities of individual sperm plasma membrane proteins in the 20 ... more densitometry. Variation in the quantities of individual sperm plasma membrane proteins in the 20 identified bands were statistically compared with the ability of spermatozoa from the same ejaculate to penetrate zona-free hamster ova. The percentages of plasma membrane protein present in 3 bands (90, 84 and 60 kD) were positively correlated with the ability of spermatozoa from the same ejaculate to fuse with zona-free hamster ova (P = 0.002, 0.01, 0.04; R = 0.53, 0.40, 0.38, respectively). The quantities of protein in 2 other bands (69 and 35 kD) were significantly but negatively correlated with the results of the zona-free hamster ova bioassay (P = 0.02, 0.01; R =-0.42,-0.37, respectively). The sperm plasma membrane profiles were quantitatively similar between the ejaculated samples and the fertile epididymal samples. Six epididymal sperm plasma membrane proteins were present in statistically different quantities in the subfertile boar sample and the 3 fertile controls. The 90 kD band positively correlated with the hamster ova bioassay in the ejaculated samples was not detected in the subfertile epididymal sperm plasma membrane sample. These results suggest that protein(s) in one or more of the 3 positively correlated ejaculated sperm plasma membrane protein bands may be involved in sperm-oocyte interaction.

Research paper thumbnail of Male Effects on Reproductive Performance

Journal of Animal Science, 1998

... The significance to bull fertility of morphologi-cally abnormal sperm. Vet. Clin. North Am. F... more ... The significance to bull fertility of morphologi-cally abnormal sperm. Vet. Clin. North Am. Food Anim. Prad. Kilgour, R. J. 1993. The relationship between ram breeding capacity and flock fertility. Theriogenology 40:277-285. algour, R. J., and R. G. Whale. 1980. ...

Research paper thumbnail of Increased testicular estradiol during the neonatal interval reduces Sertoli cell numbers

Animal Reproduction Science, 2018

Research paper thumbnail of Sertoli Cell Numbers Increase Rapidly After Aromatase Inhibition in Young Boars

Biology of Reproduction, 2009

Research paper thumbnail of The onset of puberty in colony-housed male and female titi monkeys (Plecturocebus cupreus): Possible effects of oxytocin treatment during peri-adolescent development

Hormones and Behavior, 2022

Oxytocin has been used to treat neurodevelopmental conditions in adolescent patients but possible... more Oxytocin has been used to treat neurodevelopmental conditions in adolescent patients but possible effects on reproductive development have not been well investigated. The effects of daily intra-nasal oxytocin treatment (12-18 months of age) on puberty and fertility were studied in colony-housed, male and female titi monkeys (Plecturocebus cupreus). Body weight, urinary conjugated pregnanes and estrogens (defining cyclicity) in females, and androgens and sperm in urine of in males, were measured from 1 to 3 years of age to detect puberty. Serum testosterone was also measured in males at 13, 23 and 33 months of age and hemi-castration at 3 years of age enabled assessment of testicular morphometry and oxytocin receptor expression. An oxytocin treatment*time interaction suggested a minor, transient suppression in weight gain after treatment ended. Note that females weighed 10% less across all ages. Oxytocin-treated females exhibited early, spurious ovulations but neither regular cyclicity (≈30 months) nor pregnancies were affected by treatment. Oxytocin did not affect the pubertal increase in urinary androgen or the first appearance of sperm, which occurred as early as 15 months of age. Treatment did delay the puberty-associated rise in serum testosterone in males. All males were pubertal by 22 months and all females by 32 months of age. Although no major male or female fertility outcome was observed, oxytocin demonstrated some physiological effects through a delay of testosterone secretion in males, induction of precocious ovulation in females, and a suppression of general weight gain for the months following treatment.

Research paper thumbnail of Gene Expression in Porcine Bulbourethral Glands

Research paper thumbnail of Gender disparity in survival of early porcine fetuses due to altered androgen receptor or associated U2 spliceosome component

Scientific Reports, Sep 11, 2023

Research paper thumbnail of Are testicular cortisol and WISP2 involved in estrogen-regulated Sertoli cell proliferation?

Animal Reproduction Science, Aug 1, 2019

Research paper thumbnail of Multiple Forms of Aromatase in Suiformes: Tissue Distribution, Regulation, and Functional Significance

Oxford University Press eBooks, Oct 19, 2012

Research paper thumbnail of Trichloroethylene metabolism in the rat ovary reduces oocyte fertilizability

Chemico-Biological Interactions, Oct 1, 2007

Research paper thumbnail of In vitro fertilization after in vivo treatment of rats with three reproductive toxicants☆

Reproductive Toxicology, 2000

Research paper thumbnail of In vivo exposure of female rats to toxicants may affect oocyte quality

Reproductive Toxicology, Jun 1, 2003

Research paper thumbnail of Effect of Male Accessory Gland Secretions on Sensitivity of Porcine Sperm Acrosomes to Cold Shock, Initiation of Motility and Loss of Cytoplasmic Droplets

Journal of Animal Science, May 1, 1985

Research paper thumbnail of Adsorption of seminal plasma proteins by boar spermatozoa

Theriogenology, Oct 1, 1990

Research paper thumbnail of The monomeric G-protein RhoA is widely expressed in mammalian sperm, but does not play a role in actin cytoskeletal reorganization in porcine sperm

Research paper thumbnail of Is there a sire-dam interaction in sperm fertilizing potential?

California Agriculture, Nov 1, 2001

Research paper thumbnail of Inhibition of Sperm Motility by Bovine Serum Components1

Biology of Reproduction, Mar 1, 1990

Research paper thumbnail of Potential role of alphav and beta1 integrins as oocyte adhesion molecules during fertilization in pigs

Reproduction, Sep 1, 2000

Research paper thumbnail of Comparison of techniques for selection of motile spermatozoa

Fertility and Sterility, Feb 1, 1985

Procedures to separate motile sperm with high rates of recovery may have clinical application in ... more Procedures to separate motile sperm with high rates of recovery may have clinical application in in vitro fertilization and intrauterine insemination in increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. A two-step continuous Percoll gradient was an effective means of separating motile sperm which also had enhanced ability to penetrate zona-free hamster ova. However, the requirement for a high-speed centrifuge and rotor makes the procedure impractical in many cases. A one-step discontinuous Percoll gradient was also effective in separating a population of motile sperm. Comparison of the discontinuous Percoll gradient with other techniques for separation of motile sperm indicated the discontinuous Percoll gradient had advantages in terms of recovery, enhancement of motility, and increased ability to penetrate zona-free hamster ova. The velocity of selected sperm was not significantly different among techniques. The one-step discontinuous Percoll gradient appears to have value both for increasing homogeneity of human sperm populations used for basic research and in clinical practice for male subfertility.

Research paper thumbnail of Factors Affecting Human Sperm Penetration of Zona-Free Hamster Ova

The Journal of Urology, Nov 1, 1983

Research paper thumbnail of Boar sperm plasma membrane protein profile: Correlation with the zona-free hamster ova bioassay

Theriogenology, 1994

densitometry. Variation in the quantities of individual sperm plasma membrane proteins in the 20 ... more densitometry. Variation in the quantities of individual sperm plasma membrane proteins in the 20 identified bands were statistically compared with the ability of spermatozoa from the same ejaculate to penetrate zona-free hamster ova. The percentages of plasma membrane protein present in 3 bands (90, 84 and 60 kD) were positively correlated with the ability of spermatozoa from the same ejaculate to fuse with zona-free hamster ova (P = 0.002, 0.01, 0.04; R = 0.53, 0.40, 0.38, respectively). The quantities of protein in 2 other bands (69 and 35 kD) were significantly but negatively correlated with the results of the zona-free hamster ova bioassay (P = 0.02, 0.01; R =-0.42,-0.37, respectively). The sperm plasma membrane profiles were quantitatively similar between the ejaculated samples and the fertile epididymal samples. Six epididymal sperm plasma membrane proteins were present in statistically different quantities in the subfertile boar sample and the 3 fertile controls. The 90 kD band positively correlated with the hamster ova bioassay in the ejaculated samples was not detected in the subfertile epididymal sperm plasma membrane sample. These results suggest that protein(s) in one or more of the 3 positively correlated ejaculated sperm plasma membrane protein bands may be involved in sperm-oocyte interaction.

Research paper thumbnail of Male Effects on Reproductive Performance

Journal of Animal Science, 1998

... The significance to bull fertility of morphologi-cally abnormal sperm. Vet. Clin. North Am. F... more ... The significance to bull fertility of morphologi-cally abnormal sperm. Vet. Clin. North Am. Food Anim. Prad. Kilgour, R. J. 1993. The relationship between ram breeding capacity and flock fertility. Theriogenology 40:277-285. algour, R. J., and R. G. Whale. 1980. ...

Research paper thumbnail of Increased testicular estradiol during the neonatal interval reduces Sertoli cell numbers

Animal Reproduction Science, 2018

Research paper thumbnail of Sertoli Cell Numbers Increase Rapidly After Aromatase Inhibition in Young Boars

Biology of Reproduction, 2009

Research paper thumbnail of The onset of puberty in colony-housed male and female titi monkeys (Plecturocebus cupreus): Possible effects of oxytocin treatment during peri-adolescent development

Hormones and Behavior, 2022

Oxytocin has been used to treat neurodevelopmental conditions in adolescent patients but possible... more Oxytocin has been used to treat neurodevelopmental conditions in adolescent patients but possible effects on reproductive development have not been well investigated. The effects of daily intra-nasal oxytocin treatment (12-18 months of age) on puberty and fertility were studied in colony-housed, male and female titi monkeys (Plecturocebus cupreus). Body weight, urinary conjugated pregnanes and estrogens (defining cyclicity) in females, and androgens and sperm in urine of in males, were measured from 1 to 3 years of age to detect puberty. Serum testosterone was also measured in males at 13, 23 and 33 months of age and hemi-castration at 3 years of age enabled assessment of testicular morphometry and oxytocin receptor expression. An oxytocin treatment*time interaction suggested a minor, transient suppression in weight gain after treatment ended. Note that females weighed 10% less across all ages. Oxytocin-treated females exhibited early, spurious ovulations but neither regular cyclicity (≈30 months) nor pregnancies were affected by treatment. Oxytocin did not affect the pubertal increase in urinary androgen or the first appearance of sperm, which occurred as early as 15 months of age. Treatment did delay the puberty-associated rise in serum testosterone in males. All males were pubertal by 22 months and all females by 32 months of age. Although no major male or female fertility outcome was observed, oxytocin demonstrated some physiological effects through a delay of testosterone secretion in males, induction of precocious ovulation in females, and a suppression of general weight gain for the months following treatment.