Claudia Bevilacqua - Academia.edu (original) (raw)

Uploads

Papers by Claudia Bevilacqua

HAL (Le Centre pour la Communication Scientifique Directe), 2019

HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. La comparaison des profils miARN des globules gras du lait et des cellules épithéliales mammaires suggèrent une distribution différente selon les compartiments cellulaires

HAL (Le Centre pour la Communication Scientifique Directe), Nov 20, 2014

Comparaison de differentes sources d'ARN pour analyser le transcriptome mammaire bovin par RN... more Comparaison de differentes sources d'ARN pour analyser le transcriptome mammaire bovin par RNAseq. 13. Journees de l'Animation transversale "Glande mammaire, lait"

HAL (Le Centre pour la Communication Scientifique Directe), Nov 15, 2011

HAL (Le Centre pour la Communication Scientifique Directe), Oct 21, 2009

HAL (Le Centre pour la Communication Scientifique Directe), Mar 15, 2005

Authors' original file for figure 4

Authors' original file for figure 5

Authors' original file for figure 8

Les globules gras comme source non-invasive de microARN mammaires. 23. Rencontres autour des Rech... more Les globules gras comme source non-invasive de microARN mammaires. 23. Rencontres autour des Recherches sur les Ruminants

The objective of this study was to examine the cow mammary gland transcriptome using RNA-Sequenci... more The objective of this study was to examine the cow mammary gland transcriptome using RNA-Sequencing by comparing five different cellular fractions: mammary gland tissue (MGT), milk somatic cells (SC), laser microdissected mammary epithelial cells (LCMEC), milk fat globules (MFG) and epithelial cells from milk (MEC). These samples were collected from one Normande and two Jersey cows. An average of 32-million short-sequence reads was obtained for each sample, and were mapped to the annotated UMD3.1 bovine genome assembly. In all samples 60-70% of the reads were categorized as mapped reads, with the exception of one MFG and one MEC sample that mapped 44.6% and 32.7% reads, respectively. Gene expression in SC and LCMEC showed the highest correlation with MGT, ranging from 0.99 to 0.92. In general, all the highly expressed genes such as caseins (CSN1S1, CSN1S2, CSN2, CSN3) and lactoglobulins (BLG, LALBA) showed the same expression profile in MGT, SC and LCMEC. However, MFG and MEC showed a different profile, where PLIN2, TBA1B, HMOX1, S100A11 and RL10 genes were highly expressed. These genes represent markers of lipid accumulation and immunity. MFG membrane contains specific components such as phospholipids, proteins and glycophospholipids that are known to have an affinity for the bacterial cell surface. Analyzing all the MFG reads not mapping to the bovine genome (17-million), 500 different kinds of bacteria were identified. Milk SC were the most representative sample of the mammary gland transcriptome and can be used as an effective and easy way to study gene expression in the mammary gland.

$Research paper thumbnail of Génoprotéomique : une approche intégrée pour analyser la fraction protéique du lait de souris$

HAL (Le Centre pour la Communication Scientifique Directe), Apr 21, 2008

$Research paper thumbnail of Genoproteomics: an integrated approach to analyze the protein fraction of mouse milk$

HAL (Le Centre pour la Communication Scientifique Directe), 2007

Le Centre pour la Communication Scientifique Directe - HAL - memSIC, Nov 20, 2014

Managing Salmonella enterica Enteritidis (SE) carriage in chicken is necessary to ensure human fo... more Managing Salmonella enterica Enteritidis (SE) carriage in chicken is necessary to ensure human food safety and enhance chicken breeding viability. Salmonella can contaminate poultry products, causing human foodborne disease and economic losses for farmers. Both genetic selection for a decreased carriage and gut microbiota modulation strategies could reduce Salmonella propagation in farms.Two-hundred and twenty animals from the White Leghorn inbred lines N and 61 were raised together on floor, infected by SE at 7 days of age, transferred into isolators to prevent oro-fecal recontamination and euthanized at 19 days. Caecal content DNA was used to measure individual Salmonella counts (ISC) by droplet digital PCR. A RNA sequencing approach was used to measure gene expression levels in caecal tonsils after infection of 48 chicks with low or high ISC.The analysis between lines identified 7516 differentially expressed genes (DEGs) corresponding to 62 enriched Gene Ontology (GO) Biological ...

Number of differently expressed genes common between the different comparisons (Persistent subcli... more Number of differently expressed genes common between the different comparisons (Persistent subclinical endometritis vs Healthy, Persistent subclinical endometritis vs Recovery, Recovery vs Healthy), in each cell type group (Glandular Epithelial, Luminal Epithelial, Stroma)