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Papers by Bhupendra Mistry

Schiff base (E)-2-methyl-N-(tetrazolo[1,5-a]quinolin-4-ylmethylene)aniline (QMA) was synthesized,... more Schiff base (E)-2-methyl-N-(tetrazolo[1,5-a]quinolin-4-ylmethylene)aniline (QMA) was synthesized, and its inhibitive effect for mild steel in 1N HCl solution was investigated by weight loss measurement and electrochemical tests. From the weight loss measurements and electrochemical tests, it was observed that the inhibition efficiency increases with the increase in the Schiff base concentration and reaches a maximum at the optimum concentration. This is further confirmed by the decrease in corrosion rate. It is found that the system follows Langmuir adsorption isotherm.

Food Control, 2021

Helium (He) is a non-renewable resource, the second most abundant element, and a valuable commodi... more Helium (He) is a non-renewable resource, the second most abundant element, and a valuable commodity due to its unique physical and chemical properties. Most industrial He is recovered from a limited number of natural gas (NG) reservoirs containing low concentrations of He by cryogenic distillation of the off-gases from nitrogen rejection units (NRU) in liquefied NG (LNG) plants. The conventional processes for He recovery Publications included in this thesis M. Abdul Quader, Thomas E. Rufford, and Simon Smart, "Modeling and cost analysis of helium recovery using combined-membrane process configurations", Separation and Purification Technology, 2020, Volume 236, Pages 116269.incorporated as Chapter 4. Contributors Statement of contribution M. Abdul Quader (Candidate) Concept and design (75%) Analysis and interpretation of data (75%) Drafting and writing (75%) Thomas E. Rufford Concept and design (10%) Analysis and interpretation of data (10%) Drafting and writing (10%) Simon Smart Concept and design (15%) Analysis and interpretation of data (15%)

Industrial Crops and Products, 2017

International Journal of Case Reports and Images (IJCRI) is an international, peer reviewed, mont... more International Journal of Case Reports and Images (IJCRI) is an international, peer reviewed, monthly, open access, online journal, publishing high-quality, articles in all areas of basic medical sciences and clinical specialties. Aim of IJCRI is to encourage the publication of new information by providing a platform for reporting of unique, unusual and rare cases which enhance understanding of disease process, its diagnosis, management and clinico-pathologic correlations. IJCRI publishes Review Articles, Case Series, Case Reports, Case in Images, Clinical Images and Letters to Editor.

Bioresource Technology, 2018

Remediation of engineered-nanomaterials is an up-coming major environmental concern. This study d... more Remediation of engineered-nanomaterials is an up-coming major environmental concern. This study demonstrates adsorptive-remediation of cobalt oxide nanoparticles (CoO NPs) from the water. The -cellulose-fibers were extracted from waste-paper biomass (WP-CFs) and magnetized with Fe 3 O 4 NPs (M-WP-CFs). The XRD, FT-IR, and TGA were performed for detailed characterization of the newly developed bioadsorbent. The M-WP-CFs was then applied for adsorptive remediation of CoO NPs. The adsorptive kinetics of CoO NPs adsorption onto the M-WP-CFs reveals the pseudo-second-order model. The various adsorption isotherm studies revealed Langmuir is a best-fit isotherm. A prominently high adsorption capacity q m (1567 mg/g) corroborated extraordinary adsorptive potential of M-WP-CFs. Furthermore, CoO NPs were adsorbed onto M-WP-CFs were analyzed by the XPS, VSM, and TEM. Therefore, this study gave rise WP biomass extracted and rapidly-separable nano-biocomposite of 'M-WP-CFs' with a high-capacity for CoO NPs remediation, and can be further applied in remediation of several other engineered-nanomaterials.

Chinese Chemical Letters, 2017

A new series of multi-heterocyclic Schiff base was constructed starting from 4 0-(imidazol-1-yl)a... more A new series of multi-heterocyclic Schiff base was constructed starting from 4 0-(imidazol-1-yl)acetophenone which was converted to its 2-bromoethanone precursor which on cyclic condensation with thiourea yielded final thiazol-2-amine intermediate (3) to be reacted with substituted aldehydes to generate final imidazolylphenylheterocyclic-2-ylmethylenethiazole-2-amines (4a-4i). New Schiff base was investigated for their in vitro cytotoxic efficacies against a panel of three human cancer cell lines namely, MCF7 (human breast cancer), HCT116 (human colon cancer), and DU145 (human prostate cancer) and one normal skin fibroblast (SF). Most of these synthetic derivatives shown important cytotoxic actions against individual carcinoma cell line collections, but weak actions against SF, which is as anticipated. Observations of SAR suggested that the difference in the characteristics of substituents attached to the Schiff base function leads to the interesting variations within pharmacological effects of resultant molecular systems. Structural analysis performed using FT-IR, 1 H NMR, 13 C NMR spectroscopy and CHN analysis for final potent anticancer Schiff base, which warrant further investigations.

BioFactors, 2017

Aspartame is widely used artificial sweeteners as food additives. Several researchers have pointe... more Aspartame is widely used artificial sweeteners as food additives. Several researchers have pointed that the controversial report on the use of aspartame over more than decades. Omega-3 fatty acids are essential and unsaturated fatty acids, and it plays a remarkable role in vision, intelligence, neural development, and metabolism of neurotransmitters. Therefore, the present study was aimed to investigate the effect of omega-3 fatty acids on aspartame treated renal cells. Experimental groups were divided into three such as sham control, aspartame treated, and aspartame with omega-3 fatty acids. Cell viability was determined by sulforhodamine-b assay and flow cytometric analysis. The experimental results showed that the aspartame induced altered cell viability were reduced following treatment of aspartame with omega-3 fatty acids. Altered cell morphology was recovered by omega-3 fatty acids. DNA damage appeared in the highest concentration of aspartame used in this study. DNA damage characteristics such as comet tail and tiny head sections did not appear in the omega-3 fatty acids treated cells. Several microvilli and vesicular structures were found in aspartame treated cells. Altered morphology such as rounding, microvilli, and formation of domelike structures did not appear in the omega-3 fatty acids with aspartame treated cells. Caspase-3 mRNA and protein expression were increased in aspartame treated cells, and these levels were reduced following omega-3 fatty acids treatment. Taking all these data together, it is suggested that the omega-3 fatty acids may be a therapeutic agent to reduce the aspartame induced biochemical and morphological alterations in normal renal cells.

Transactions of the Indian Institute of Metals, 2016

Two synthesized Schiff base derivatives were analyzed as corrosion inhibitors for mild steel in 1... more Two synthesized Schiff base derivatives were analyzed as corrosion inhibitors for mild steel in 1 N HCl solution by a series of known techniques such as electrochemical impedance spectroscopy, weight loss, potentiodynamic polarization, linear polarization and scanning electron microscopic at different inhibitor concentrations. The effects demonstrate that two Schiff base derivatives are excellent inhibitors, and inhibition efficiency accepts the order: SB1 [ SB2. The adsorption of each inhibitor on metal surface obeys Langmuir adsorption isotherm. Two Schiff base derivatives acted as mixed-type inhibitors. The surface of the mild steel was examined by scanning electron microscope in the absence and presence of Schiff base derivatives.

Amino acids, Aug 1, 2017

β-Alanine is a non-essential amino acid and presents as a major component of various sports suppl... more β-Alanine is a non-essential amino acid and presents as a major component of various sports supplements. It is a non-proteogenic amino acid, formed in vivo by degradation of carnosine, anserine, balenine, and dihydrouracil. The present study was aimed at investigating the anti-tumor effects of β-alanine in renal and cervical tumor cells. Sulforhodamine-B assay and flow cytometric analysis were used to measure cell viability. Lactate dehydrogenase (LDH) expression was analyzed using FITC-conjugated fluorescent antibody. The cellular adenosine triphosphate (ATP) content was measured using bioluminescence method. Cell migration was determined by the simple standard-scratch method. β-Alanine reduced renal and cervical cell growth significantly. Percentage of inhibition of renal and cervical tumor cells was increased at higher concentration of β-alanine. LDH expression and ATP content were significantly reduced in renal and cervical tumor cells in a dose-dependent manner. Renal and cervi...

Journal of Food Measurement and Characterization, 2016

and catalase enzyme activities were significantly reduced in a dose-and time-dependent manner. Ta... more and catalase enzyme activities were significantly reduced in a dose-and time-dependent manner. Taking all these data together, it is concluded that aspartame may induce oxidative stress in the MDCK cells.

Medicinal Chemistry Research, 2016

Piperazine derivatives bearing different electronwithdrawing and electron-donating functional gro... more Piperazine derivatives bearing different electronwithdrawing and electron-donating functional groups were linked to the well-known isoquinoline alkaloid derivative, berberine via efficient organic transformations. The entire target berberine-based analogues were examined for their in vitro antioxidant potency using 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid bioassays, and anticancer activities using sulforhodamine B assay against HeLa and CaSki cervical cancer cell lines in addition to the cytotoxicity using Madin-Darby canine kidney non-cancer cell lines and, ascorbic acid and berberine used as a control for antioxidant and anticancer activities, respectively. Bioassay results revealed that newer compounds were more active against CaSki and HeLa cell lines with therapeutic indices better than that of parent berberine and showed tolerable cytotoxicity to the normal cells. A final analogue 5a with 4-methylpiperazine substituent indicated most significant anticancer potency with a therapeutic index of 58.53 (HeLa) and 48.76 (CaSki), followed by those bearing meta-chloropiperazine rings with a therapeutic index of 41.83 (HeLa) and 47.35 (CaSki), respectively.In addition, newly synthesized analogues exerted a significant radical scavenging activity against 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid cation with IC 50 values of 8.917 µg/mL, and were good to moderate scavengers of 2,2-diphenyl-1-picrylhydrazyl radical with IC 50 values of 25.40 µg/mL. Synthesized compound was characterized using several techniques, fourier transform infrared spectroscopy, 1 H nuclear magnetic resonance, 13 C nuclear magnetic resonance, mass spectroscopy and elemental (CHN) analyses.

International Journal of Biological Macromolecules, 2016

Carnosine is a natural antioxidant dipeptide that is highly concentrated in muscles and brain. Th... more Carnosine is a natural antioxidant dipeptide that is highly concentrated in muscles and brain. The present study investigated the effect of carnosine cell growth inhibition and activation of the caspase-3 enzyme under cell co-culture system. Renal carcinoma and normal cells were co-cultured to provide three-dimensional views for the experimental analyses. Carnosine inhibited renal cancer cell growth up to 40%, whereas it was 25% in normal cells. Caspase-3 enzyme activity corresponded to the appearance of immunofluorescence in the cytoplasm using the caspase-3 antibody. Caspase-3 enzyme activity gradually increased in renal carcinoma cells in a concentration-dependent manner. The increased immunofluorescence and fluorescent detection of caspase-3 indicated the occurrence of apoptosis. The binding affinity of carnosine with caspase-3 subunit was confirmed by In silico docking study and glide energy was -5.2kcal/mol. Taking all these data together, it is suggested that the carnosine may be a potential antiproliferative agent in renal carcinoma tumor.

Journal of Chemical Research, 2016

The 9-demethylated derivative of the isoquinoline alkaloid berberine was derivatised in its isoqu... more The 9-demethylated derivative of the isoquinoline alkaloid berberine was derivatised in its isoquinoline moiety using enamines derived from formaldehyde and morpholine, piperidine, carbazole and six variously substituted piperazines to form Mannich base products which were evaluated for their in vitro biological effects. Standard tests determined their radical scavenging potential and their ferric reducing antioxidant power (FRAP). Cancerous growth inhibitory efficacies were assessed using cervical cancer cell lines HeLa and CaSki and their cytotoxicities towards normal cell lines were evaluated using Madin–Darby canine kidney (MDCK) cell lines. Piperazine derivatives bearing a heterocyclic nitrogen substituent such as a pyridyl or a pyrimidyl ring were the most active antioxidant and anticancer agents. A carbazole moiety attached to the berberine core also demonstrated excellent inhibitory effects on cancerous cells.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Jan 23, 2016

Synthesis of 7-(4-bromobutoxy)-5-hydroxy-2-phenyl-4H-chromen-4-one intermediate treating chrysin ... more Synthesis of 7-(4-bromobutoxy)-5-hydroxy-2-phenyl-4H-chromen-4-one intermediate treating chrysin with 1,4-dibromobutane facilitated combination of chrysin with a wide range of piperazine moieties which were equipped via reacting the corresponding amines with bis(2-chloroethyl)amine hydrochloride in diethylene glycol monomethyl ether solvent. Free radical scavenging potential of prepared products was analyzed in vitro adopting DPPH and ABTS bioassay in addition to the evaluation of in vitro anticancer efficacies against cervical cancer cell lines (HeLa and CaSki) and an ovarian cancer cell line SK-OV-3 using SRB assay. Bearable toxicity of 7a-w was examined employing Madin-Darby canine kidney (MDCK) cell line. In addition, cytotoxic nature of the presented compounds was inspected utilizing Human bone marrow derived mesenchymal stem cells (hBM-MSCs). Overall, 7a-w indicated remarkable antioxidant power in scavenging DPPH(·) and ABTS(·+), particularly analogs 7f, 7j, 7k, 7l, 7n, 7q, 7v...

Anti-Cancer Agents in Medicinal Chemistry, 2016

A new series of 9-O-3-(1-piperazinyl/morpholinyl/piperidinyl)pentyl-berberines has been efficient... more A new series of 9-O-3-(1-piperazinyl/morpholinyl/piperidinyl)pentyl-berberines has been efficiently formulated via coupling 1,5-dibromopentane with berberrubine, This was obtained by treating berberine in a vacuum oven at optimum temperature and pressure. Nucleophilic substitution of a variety of substituted piperazines, morpholine, carbazole and piperidine furnished analogues 5a-i. All final compounds were evaluated for their in vitro antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) bioassays. Also, cancer cell inhibitory potential of titled compounds was screened for cervical cancer, HeLa and CaSki employing SRB assay in terms of cytotoxicity. A minimum inhibitory concentration of 5a-i towards normal cells was studied using Madin-Darby canine kidney (MDCK) cell lines. Final compounds with morpholine and piperidine substituents showed excellent free radical scavenging efficacies in both DPPH and ABTS bioassays, respectively. The presence of naphthyl, benzhydryl, benzoyl, furoyl and heterocyclic rings on the piperazine system was essential to exert anticipated cytotoxic effects against cancer cell lines. The structure of the final compounds was adequately confirmed via spectroscopic techniques and further elemental analysis and characterization of physical properties.

Journal of Saudi Chemical Society, 2017

Benzothiazole moieties substituted with various functional groups were utilized to link with the ... more Benzothiazole moieties substituted with various functional groups were utilized to link with the isoquinoline alkaloid berberine through a pentyl side chain. Entitled analogs were screened for antioxidant potency using the DPPH and ABTS bioassays and for their in vitro anticancer activities against HeLa, CaSki (cervical cancer), and SK-OV-3 (ovarian cancer) cell lines using the SRB bioassay. The compounds were evaluated for their toxicity to the Madin-Darby canine kidney (MDCK) cell line. The final compounds demonstrated significant antioxidant potency with IC 50 levels of 13.03-24.50 lg/mL and 4.958-7.570 lg/mL in the DPPH and ABTS radical scavenging bioassays, respectively. The 5e analog with a methoxy functional group and the 5m analog with a cyano functional group had the most significant DPPH and ABTS radical scavenging activities, respectively. Moreover, the 5m cyano-based analog had the highest potency against all cancer cell lines, with IC 50 levels of 5.474, 5.311, and 32.61 lg/mL against the HeLa, CaSki, and SK-OV-3 cell lines, respectively. All the synthesized compounds were characterized by IR, 1 H NMR, 13 C NMR spectroscopy and elemental analysis.

제 8회 발효한약국제심포지움 및 한국자원식물학회 추계학술대회, Sep 1, 2014

Saudi Journal of Biological Sciences, 2017

A new Mannich base series of piperazine linked berberine analogues was furnished in this study to... more A new Mannich base series of piperazine linked berberine analogues was furnished in this study to screen the antioxidant and anticancer potential of the resultant analogues. Alkoxy group at a C-9 position of berberine was converted to hydroxyl functionality to enhance the ability of final scaffolds binding to the target of drug action mainly through hydrophobic effect, conjugation effect, whereas Mannich base functionality was introduced on the C-12 position of berberine. Scaffolds were investigated for their free radical scavenging antioxidant potential in FRAP and DPPH assay, whereas tested to check their Fe +3 reducing power in ABTS assay. The radical scavenging potential of the final derivatives 4a-j was found excellent with IC 50 s, <13 lg/mL and < 8 lg/mL in DPPH and ABTS assay, respectively, whereas some analogues showed significant Fe +3 reducing power with absorption at around 2 nm in the FRAP assay. Anticancer effects of titled compounds were inspected against cervical cancer cell line Hela and Caski adapting SRB assay, in which analogues 4a-j presented <6 lg/mL of IC 50 s, and >30 of therapeutic indices, thus exerting low cytotoxic values against Malin-Darby canine kidney (MDCK) cell lines at CC 50 s >125 lg/mL. Hence, from the bioassay outcomes it can be stated that these analogues are dual active agents as the scavengers of reactive oxygen species and inhibitors of the cancerous cells as compounds with halogen functional group have overall good pharmacological potential in assays studied in this research. Correct structure of the final compounds was adequately confirmed on the basis of FT-IR and 1 H NMR as well as elemental analyses.

Journal of the Iranian Chemical Society, 2015

Isoquinoline alkaloids possess versatile biological activities. Hence, in the current research an... more Isoquinoline alkaloids possess versatile biological activities. Hence, in the current research an effort has been made to improve structurally important part of isoquinoline alkaloid berberine because it is recognized as the marking compound for the crude drugs. Synthesis of rationalized berberine has been performed via substituting various piperazine moieties bearing disubstituted electron withdrawing and electron donating groups to the berberine core via Mannich reaction. Intended scaffolds were inspected for their in vitro antioxidant potential using different bioassays, FRAP, DPPH and ABTS as well as anticancer efficacies against cervical cancer cell lines HeLa, CaSki adapting SRB assay. Also, an inspection of the cytotoxic nature of titled analogues has been carried out towards Madin-Darby canine kidney (MDCK) cell lines. Radical scavenging potential of the final derivatives 4a–i was found to be excellent with IC50s, <20 and <12 µg/mL in DPPH and ABTS assay, respectively, whereas some dichlorophenyl piperazine analogues revealed important Fe3+ decreasing power with absorption at around 2 nm in FRAP assay. Moreover, compounds 4a–i appeared with significant inhibitors of the cervical cancer cell lines HeLa and CaSki with IC50s ranging 4.346–6.321 and 3.408–6.081 µg/mL, with low level of cytotoxic values and higher therapeutic indices ranging 20.42–42.45 and 21.23–43.25, respectively. Therefore, from the bioassay results it can be mentioned that these analogues are effective double agents as the scavengers of reactive oxygen species and inhibitors of the cancerous cells. The correct structure of the final compounds was adequately confirmed on the basis of FT-IR, 1H NMR and mass spectroscopy data as well as elemental analyses.

Arabian Journal of Chemistry, 2017

Azide-alkyne cycloaddition was attempted to generate a flavanone hesperetin based phenyl substitu... more Azide-alkyne cycloaddition was attempted to generate a flavanone hesperetin based phenyl substituted 1,2,3-triazolyls as semi-synthetic natural product derivatives utilizing coppercatalyzed click chemistry. All final compounds were analyzed for their in vitro antioxidant abilities using DPPH and ABTS bioassay. Moreover, cancerous cell inhibitory prospect of titled compounds was screened against cervical cancer cell lines, HeLa and CaSki and an ovarian cancer cell line SK-OV-3 implementing SRB assay. Bearable toxicity of 6a-s was examined employing Madin-Darby canine kidney (MDCK) non-cancer cell line. Overall, 6a-s indicated remarkable antioxidant power in scavenging DPPH Å and ABTS Å+ ; particularly, an analog 6o with meta-methoxy substituent showed most potent radical scavenging activity, whereas scaffolds 6d with para-fluoro, 6k with ortho-methyl, and 6o with meta-methoxy performed excellently in inhibiting both the cervical cancer cell lines and analog 6q with meta-trifluoromethyl substituent expressed excellent sensitivity toward ovarian cancer cell line. From the structure-activity point of view, nature and position of the electron withdrawing and electron donating functional groups on the phenyl ring attached to the tria

Research on Chemical Intermediates, 2014

The effect of novel synthesized Schiff base, namely (Z)-4-chloro-N-((2-chloroquinolin-3-yl)methyl... more The effect of novel synthesized Schiff base, namely (Z)-4-chloro-N-((2-chloroquinolin-3-yl)methylene)aniline (4-CCMA) on the corrosion of mild steel in 1 N HCl was investigated using weight loss, potentiodynamic polarization, linear polarization, electrochemical impedance spectroscopy, and scanning electron microscopy. The experimental results showed that 4-CCMA revealed good corrosion inhibition and that the inhibition efficiency increases with the increase of concentration of inhibitor to attain 99.04 % for 4-CCMA at 25 ppm. Polarization measurements suggest that 4-CCMA acts as a mixed-type inhibitor. Electrochemical impedance spectroscopy measurements show an increase of the transfer resistance with the inhibitor concentration. The temperature effect on the corrosion behavior of mild steel in 1 N HCl without and with the inhibitor at 5–25 ppm were studied in the temperature range from 298 to 318 K, and the associated activation energy has been determined. Adsorption of 4-CCMA on the mild steel surfaces in 1 N HCl follows the Langmuir adsorption isotherm model.

Schiff base (E)-2-methyl-N-(tetrazolo[1,5-a]quinolin-4-ylmethylene)aniline (QMA) was synthesized,... more Schiff base (E)-2-methyl-N-(tetrazolo[1,5-a]quinolin-4-ylmethylene)aniline (QMA) was synthesized, and its inhibitive effect for mild steel in 1N HCl solution was investigated by weight loss measurement and electrochemical tests. From the weight loss measurements and electrochemical tests, it was observed that the inhibition efficiency increases with the increase in the Schiff base concentration and reaches a maximum at the optimum concentration. This is further confirmed by the decrease in corrosion rate. It is found that the system follows Langmuir adsorption isotherm.

Food Control, 2021

Helium (He) is a non-renewable resource, the second most abundant element, and a valuable commodi... more Helium (He) is a non-renewable resource, the second most abundant element, and a valuable commodity due to its unique physical and chemical properties. Most industrial He is recovered from a limited number of natural gas (NG) reservoirs containing low concentrations of He by cryogenic distillation of the off-gases from nitrogen rejection units (NRU) in liquefied NG (LNG) plants. The conventional processes for He recovery Publications included in this thesis M. Abdul Quader, Thomas E. Rufford, and Simon Smart, "Modeling and cost analysis of helium recovery using combined-membrane process configurations", Separation and Purification Technology, 2020, Volume 236, Pages 116269.incorporated as Chapter 4. Contributors Statement of contribution M. Abdul Quader (Candidate) Concept and design (75%) Analysis and interpretation of data (75%) Drafting and writing (75%) Thomas E. Rufford Concept and design (10%) Analysis and interpretation of data (10%) Drafting and writing (10%) Simon Smart Concept and design (15%) Analysis and interpretation of data (15%)

Industrial Crops and Products, 2017

International Journal of Case Reports and Images (IJCRI) is an international, peer reviewed, mont... more International Journal of Case Reports and Images (IJCRI) is an international, peer reviewed, monthly, open access, online journal, publishing high-quality, articles in all areas of basic medical sciences and clinical specialties. Aim of IJCRI is to encourage the publication of new information by providing a platform for reporting of unique, unusual and rare cases which enhance understanding of disease process, its diagnosis, management and clinico-pathologic correlations. IJCRI publishes Review Articles, Case Series, Case Reports, Case in Images, Clinical Images and Letters to Editor.

Bioresource Technology, 2018

Remediation of engineered-nanomaterials is an up-coming major environmental concern. This study d... more Remediation of engineered-nanomaterials is an up-coming major environmental concern. This study demonstrates adsorptive-remediation of cobalt oxide nanoparticles (CoO NPs) from the water. The -cellulose-fibers were extracted from waste-paper biomass (WP-CFs) and magnetized with Fe 3 O 4 NPs (M-WP-CFs). The XRD, FT-IR, and TGA were performed for detailed characterization of the newly developed bioadsorbent. The M-WP-CFs was then applied for adsorptive remediation of CoO NPs. The adsorptive kinetics of CoO NPs adsorption onto the M-WP-CFs reveals the pseudo-second-order model. The various adsorption isotherm studies revealed Langmuir is a best-fit isotherm. A prominently high adsorption capacity q m (1567 mg/g) corroborated extraordinary adsorptive potential of M-WP-CFs. Furthermore, CoO NPs were adsorbed onto M-WP-CFs were analyzed by the XPS, VSM, and TEM. Therefore, this study gave rise WP biomass extracted and rapidly-separable nano-biocomposite of 'M-WP-CFs' with a high-capacity for CoO NPs remediation, and can be further applied in remediation of several other engineered-nanomaterials.

Chinese Chemical Letters, 2017

A new series of multi-heterocyclic Schiff base was constructed starting from 4 0-(imidazol-1-yl)a... more A new series of multi-heterocyclic Schiff base was constructed starting from 4 0-(imidazol-1-yl)acetophenone which was converted to its 2-bromoethanone precursor which on cyclic condensation with thiourea yielded final thiazol-2-amine intermediate (3) to be reacted with substituted aldehydes to generate final imidazolylphenylheterocyclic-2-ylmethylenethiazole-2-amines (4a-4i). New Schiff base was investigated for their in vitro cytotoxic efficacies against a panel of three human cancer cell lines namely, MCF7 (human breast cancer), HCT116 (human colon cancer), and DU145 (human prostate cancer) and one normal skin fibroblast (SF). Most of these synthetic derivatives shown important cytotoxic actions against individual carcinoma cell line collections, but weak actions against SF, which is as anticipated. Observations of SAR suggested that the difference in the characteristics of substituents attached to the Schiff base function leads to the interesting variations within pharmacological effects of resultant molecular systems. Structural analysis performed using FT-IR, 1 H NMR, 13 C NMR spectroscopy and CHN analysis for final potent anticancer Schiff base, which warrant further investigations.

BioFactors, 2017

Aspartame is widely used artificial sweeteners as food additives. Several researchers have pointe... more Aspartame is widely used artificial sweeteners as food additives. Several researchers have pointed that the controversial report on the use of aspartame over more than decades. Omega-3 fatty acids are essential and unsaturated fatty acids, and it plays a remarkable role in vision, intelligence, neural development, and metabolism of neurotransmitters. Therefore, the present study was aimed to investigate the effect of omega-3 fatty acids on aspartame treated renal cells. Experimental groups were divided into three such as sham control, aspartame treated, and aspartame with omega-3 fatty acids. Cell viability was determined by sulforhodamine-b assay and flow cytometric analysis. The experimental results showed that the aspartame induced altered cell viability were reduced following treatment of aspartame with omega-3 fatty acids. Altered cell morphology was recovered by omega-3 fatty acids. DNA damage appeared in the highest concentration of aspartame used in this study. DNA damage characteristics such as comet tail and tiny head sections did not appear in the omega-3 fatty acids treated cells. Several microvilli and vesicular structures were found in aspartame treated cells. Altered morphology such as rounding, microvilli, and formation of domelike structures did not appear in the omega-3 fatty acids with aspartame treated cells. Caspase-3 mRNA and protein expression were increased in aspartame treated cells, and these levels were reduced following omega-3 fatty acids treatment. Taking all these data together, it is suggested that the omega-3 fatty acids may be a therapeutic agent to reduce the aspartame induced biochemical and morphological alterations in normal renal cells.

Transactions of the Indian Institute of Metals, 2016

Two synthesized Schiff base derivatives were analyzed as corrosion inhibitors for mild steel in 1... more Two synthesized Schiff base derivatives were analyzed as corrosion inhibitors for mild steel in 1 N HCl solution by a series of known techniques such as electrochemical impedance spectroscopy, weight loss, potentiodynamic polarization, linear polarization and scanning electron microscopic at different inhibitor concentrations. The effects demonstrate that two Schiff base derivatives are excellent inhibitors, and inhibition efficiency accepts the order: SB1 [ SB2. The adsorption of each inhibitor on metal surface obeys Langmuir adsorption isotherm. Two Schiff base derivatives acted as mixed-type inhibitors. The surface of the mild steel was examined by scanning electron microscope in the absence and presence of Schiff base derivatives.

Amino acids, Aug 1, 2017

β-Alanine is a non-essential amino acid and presents as a major component of various sports suppl... more β-Alanine is a non-essential amino acid and presents as a major component of various sports supplements. It is a non-proteogenic amino acid, formed in vivo by degradation of carnosine, anserine, balenine, and dihydrouracil. The present study was aimed at investigating the anti-tumor effects of β-alanine in renal and cervical tumor cells. Sulforhodamine-B assay and flow cytometric analysis were used to measure cell viability. Lactate dehydrogenase (LDH) expression was analyzed using FITC-conjugated fluorescent antibody. The cellular adenosine triphosphate (ATP) content was measured using bioluminescence method. Cell migration was determined by the simple standard-scratch method. β-Alanine reduced renal and cervical cell growth significantly. Percentage of inhibition of renal and cervical tumor cells was increased at higher concentration of β-alanine. LDH expression and ATP content were significantly reduced in renal and cervical tumor cells in a dose-dependent manner. Renal and cervi...

Journal of Food Measurement and Characterization, 2016

and catalase enzyme activities were significantly reduced in a dose-and time-dependent manner. Ta... more and catalase enzyme activities were significantly reduced in a dose-and time-dependent manner. Taking all these data together, it is concluded that aspartame may induce oxidative stress in the MDCK cells.

Medicinal Chemistry Research, 2016

Piperazine derivatives bearing different electronwithdrawing and electron-donating functional gro... more Piperazine derivatives bearing different electronwithdrawing and electron-donating functional groups were linked to the well-known isoquinoline alkaloid derivative, berberine via efficient organic transformations. The entire target berberine-based analogues were examined for their in vitro antioxidant potency using 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid bioassays, and anticancer activities using sulforhodamine B assay against HeLa and CaSki cervical cancer cell lines in addition to the cytotoxicity using Madin-Darby canine kidney non-cancer cell lines and, ascorbic acid and berberine used as a control for antioxidant and anticancer activities, respectively. Bioassay results revealed that newer compounds were more active against CaSki and HeLa cell lines with therapeutic indices better than that of parent berberine and showed tolerable cytotoxicity to the normal cells. A final analogue 5a with 4-methylpiperazine substituent indicated most significant anticancer potency with a therapeutic index of 58.53 (HeLa) and 48.76 (CaSki), followed by those bearing meta-chloropiperazine rings with a therapeutic index of 41.83 (HeLa) and 47.35 (CaSki), respectively.In addition, newly synthesized analogues exerted a significant radical scavenging activity against 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid cation with IC 50 values of 8.917 µg/mL, and were good to moderate scavengers of 2,2-diphenyl-1-picrylhydrazyl radical with IC 50 values of 25.40 µg/mL. Synthesized compound was characterized using several techniques, fourier transform infrared spectroscopy, 1 H nuclear magnetic resonance, 13 C nuclear magnetic resonance, mass spectroscopy and elemental (CHN) analyses.

International Journal of Biological Macromolecules, 2016

Carnosine is a natural antioxidant dipeptide that is highly concentrated in muscles and brain. Th... more Carnosine is a natural antioxidant dipeptide that is highly concentrated in muscles and brain. The present study investigated the effect of carnosine cell growth inhibition and activation of the caspase-3 enzyme under cell co-culture system. Renal carcinoma and normal cells were co-cultured to provide three-dimensional views for the experimental analyses. Carnosine inhibited renal cancer cell growth up to 40%, whereas it was 25% in normal cells. Caspase-3 enzyme activity corresponded to the appearance of immunofluorescence in the cytoplasm using the caspase-3 antibody. Caspase-3 enzyme activity gradually increased in renal carcinoma cells in a concentration-dependent manner. The increased immunofluorescence and fluorescent detection of caspase-3 indicated the occurrence of apoptosis. The binding affinity of carnosine with caspase-3 subunit was confirmed by In silico docking study and glide energy was -5.2kcal/mol. Taking all these data together, it is suggested that the carnosine may be a potential antiproliferative agent in renal carcinoma tumor.

Journal of Chemical Research, 2016

The 9-demethylated derivative of the isoquinoline alkaloid berberine was derivatised in its isoqu... more The 9-demethylated derivative of the isoquinoline alkaloid berberine was derivatised in its isoquinoline moiety using enamines derived from formaldehyde and morpholine, piperidine, carbazole and six variously substituted piperazines to form Mannich base products which were evaluated for their in vitro biological effects. Standard tests determined their radical scavenging potential and their ferric reducing antioxidant power (FRAP). Cancerous growth inhibitory efficacies were assessed using cervical cancer cell lines HeLa and CaSki and their cytotoxicities towards normal cell lines were evaluated using Madin–Darby canine kidney (MDCK) cell lines. Piperazine derivatives bearing a heterocyclic nitrogen substituent such as a pyridyl or a pyrimidyl ring were the most active antioxidant and anticancer agents. A carbazole moiety attached to the berberine core also demonstrated excellent inhibitory effects on cancerous cells.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Jan 23, 2016

Synthesis of 7-(4-bromobutoxy)-5-hydroxy-2-phenyl-4H-chromen-4-one intermediate treating chrysin ... more Synthesis of 7-(4-bromobutoxy)-5-hydroxy-2-phenyl-4H-chromen-4-one intermediate treating chrysin with 1,4-dibromobutane facilitated combination of chrysin with a wide range of piperazine moieties which were equipped via reacting the corresponding amines with bis(2-chloroethyl)amine hydrochloride in diethylene glycol monomethyl ether solvent. Free radical scavenging potential of prepared products was analyzed in vitro adopting DPPH and ABTS bioassay in addition to the evaluation of in vitro anticancer efficacies against cervical cancer cell lines (HeLa and CaSki) and an ovarian cancer cell line SK-OV-3 using SRB assay. Bearable toxicity of 7a-w was examined employing Madin-Darby canine kidney (MDCK) cell line. In addition, cytotoxic nature of the presented compounds was inspected utilizing Human bone marrow derived mesenchymal stem cells (hBM-MSCs). Overall, 7a-w indicated remarkable antioxidant power in scavenging DPPH(·) and ABTS(·+), particularly analogs 7f, 7j, 7k, 7l, 7n, 7q, 7v...

Anti-Cancer Agents in Medicinal Chemistry, 2016

A new series of 9-O-3-(1-piperazinyl/morpholinyl/piperidinyl)pentyl-berberines has been efficient... more A new series of 9-O-3-(1-piperazinyl/morpholinyl/piperidinyl)pentyl-berberines has been efficiently formulated via coupling 1,5-dibromopentane with berberrubine, This was obtained by treating berberine in a vacuum oven at optimum temperature and pressure. Nucleophilic substitution of a variety of substituted piperazines, morpholine, carbazole and piperidine furnished analogues 5a-i. All final compounds were evaluated for their in vitro antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) bioassays. Also, cancer cell inhibitory potential of titled compounds was screened for cervical cancer, HeLa and CaSki employing SRB assay in terms of cytotoxicity. A minimum inhibitory concentration of 5a-i towards normal cells was studied using Madin-Darby canine kidney (MDCK) cell lines. Final compounds with morpholine and piperidine substituents showed excellent free radical scavenging efficacies in both DPPH and ABTS bioassays, respectively. The presence of naphthyl, benzhydryl, benzoyl, furoyl and heterocyclic rings on the piperazine system was essential to exert anticipated cytotoxic effects against cancer cell lines. The structure of the final compounds was adequately confirmed via spectroscopic techniques and further elemental analysis and characterization of physical properties.

Journal of Saudi Chemical Society, 2017

Benzothiazole moieties substituted with various functional groups were utilized to link with the ... more Benzothiazole moieties substituted with various functional groups were utilized to link with the isoquinoline alkaloid berberine through a pentyl side chain. Entitled analogs were screened for antioxidant potency using the DPPH and ABTS bioassays and for their in vitro anticancer activities against HeLa, CaSki (cervical cancer), and SK-OV-3 (ovarian cancer) cell lines using the SRB bioassay. The compounds were evaluated for their toxicity to the Madin-Darby canine kidney (MDCK) cell line. The final compounds demonstrated significant antioxidant potency with IC 50 levels of 13.03-24.50 lg/mL and 4.958-7.570 lg/mL in the DPPH and ABTS radical scavenging bioassays, respectively. The 5e analog with a methoxy functional group and the 5m analog with a cyano functional group had the most significant DPPH and ABTS radical scavenging activities, respectively. Moreover, the 5m cyano-based analog had the highest potency against all cancer cell lines, with IC 50 levels of 5.474, 5.311, and 32.61 lg/mL against the HeLa, CaSki, and SK-OV-3 cell lines, respectively. All the synthesized compounds were characterized by IR, 1 H NMR, 13 C NMR spectroscopy and elemental analysis.

제 8회 발효한약국제심포지움 및 한국자원식물학회 추계학술대회, Sep 1, 2014

Saudi Journal of Biological Sciences, 2017

A new Mannich base series of piperazine linked berberine analogues was furnished in this study to... more A new Mannich base series of piperazine linked berberine analogues was furnished in this study to screen the antioxidant and anticancer potential of the resultant analogues. Alkoxy group at a C-9 position of berberine was converted to hydroxyl functionality to enhance the ability of final scaffolds binding to the target of drug action mainly through hydrophobic effect, conjugation effect, whereas Mannich base functionality was introduced on the C-12 position of berberine. Scaffolds were investigated for their free radical scavenging antioxidant potential in FRAP and DPPH assay, whereas tested to check their Fe +3 reducing power in ABTS assay. The radical scavenging potential of the final derivatives 4a-j was found excellent with IC 50 s, <13 lg/mL and < 8 lg/mL in DPPH and ABTS assay, respectively, whereas some analogues showed significant Fe +3 reducing power with absorption at around 2 nm in the FRAP assay. Anticancer effects of titled compounds were inspected against cervical cancer cell line Hela and Caski adapting SRB assay, in which analogues 4a-j presented <6 lg/mL of IC 50 s, and >30 of therapeutic indices, thus exerting low cytotoxic values against Malin-Darby canine kidney (MDCK) cell lines at CC 50 s >125 lg/mL. Hence, from the bioassay outcomes it can be stated that these analogues are dual active agents as the scavengers of reactive oxygen species and inhibitors of the cancerous cells as compounds with halogen functional group have overall good pharmacological potential in assays studied in this research. Correct structure of the final compounds was adequately confirmed on the basis of FT-IR and 1 H NMR as well as elemental analyses.

Journal of the Iranian Chemical Society, 2015

Isoquinoline alkaloids possess versatile biological activities. Hence, in the current research an... more Isoquinoline alkaloids possess versatile biological activities. Hence, in the current research an effort has been made to improve structurally important part of isoquinoline alkaloid berberine because it is recognized as the marking compound for the crude drugs. Synthesis of rationalized berberine has been performed via substituting various piperazine moieties bearing disubstituted electron withdrawing and electron donating groups to the berberine core via Mannich reaction. Intended scaffolds were inspected for their in vitro antioxidant potential using different bioassays, FRAP, DPPH and ABTS as well as anticancer efficacies against cervical cancer cell lines HeLa, CaSki adapting SRB assay. Also, an inspection of the cytotoxic nature of titled analogues has been carried out towards Madin-Darby canine kidney (MDCK) cell lines. Radical scavenging potential of the final derivatives 4a–i was found to be excellent with IC50s, <20 and <12 µg/mL in DPPH and ABTS assay, respectively, whereas some dichlorophenyl piperazine analogues revealed important Fe3+ decreasing power with absorption at around 2 nm in FRAP assay. Moreover, compounds 4a–i appeared with significant inhibitors of the cervical cancer cell lines HeLa and CaSki with IC50s ranging 4.346–6.321 and 3.408–6.081 µg/mL, with low level of cytotoxic values and higher therapeutic indices ranging 20.42–42.45 and 21.23–43.25, respectively. Therefore, from the bioassay results it can be mentioned that these analogues are effective double agents as the scavengers of reactive oxygen species and inhibitors of the cancerous cells. The correct structure of the final compounds was adequately confirmed on the basis of FT-IR, 1H NMR and mass spectroscopy data as well as elemental analyses.

Arabian Journal of Chemistry, 2017

Azide-alkyne cycloaddition was attempted to generate a flavanone hesperetin based phenyl substitu... more Azide-alkyne cycloaddition was attempted to generate a flavanone hesperetin based phenyl substituted 1,2,3-triazolyls as semi-synthetic natural product derivatives utilizing coppercatalyzed click chemistry. All final compounds were analyzed for their in vitro antioxidant abilities using DPPH and ABTS bioassay. Moreover, cancerous cell inhibitory prospect of titled compounds was screened against cervical cancer cell lines, HeLa and CaSki and an ovarian cancer cell line SK-OV-3 implementing SRB assay. Bearable toxicity of 6a-s was examined employing Madin-Darby canine kidney (MDCK) non-cancer cell line. Overall, 6a-s indicated remarkable antioxidant power in scavenging DPPH Å and ABTS Å+ ; particularly, an analog 6o with meta-methoxy substituent showed most potent radical scavenging activity, whereas scaffolds 6d with para-fluoro, 6k with ortho-methyl, and 6o with meta-methoxy performed excellently in inhibiting both the cervical cancer cell lines and analog 6q with meta-trifluoromethyl substituent expressed excellent sensitivity toward ovarian cancer cell line. From the structure-activity point of view, nature and position of the electron withdrawing and electron donating functional groups on the phenyl ring attached to the tria

Research on Chemical Intermediates, 2014

The effect of novel synthesized Schiff base, namely (Z)-4-chloro-N-((2-chloroquinolin-3-yl)methyl... more The effect of novel synthesized Schiff base, namely (Z)-4-chloro-N-((2-chloroquinolin-3-yl)methylene)aniline (4-CCMA) on the corrosion of mild steel in 1 N HCl was investigated using weight loss, potentiodynamic polarization, linear polarization, electrochemical impedance spectroscopy, and scanning electron microscopy. The experimental results showed that 4-CCMA revealed good corrosion inhibition and that the inhibition efficiency increases with the increase of concentration of inhibitor to attain 99.04 % for 4-CCMA at 25 ppm. Polarization measurements suggest that 4-CCMA acts as a mixed-type inhibitor. Electrochemical impedance spectroscopy measurements show an increase of the transfer resistance with the inhibitor concentration. The temperature effect on the corrosion behavior of mild steel in 1 N HCl without and with the inhibitor at 5–25 ppm were studied in the temperature range from 298 to 318 K, and the associated activation energy has been determined. Adsorption of 4-CCMA on the mild steel surfaces in 1 N HCl follows the Langmuir adsorption isotherm model.