Bishun Deo Prasad - Academia.edu (original) (raw)
Papers by Bishun Deo Prasad
International Journal of Plant & Soil Science
The present study was performed to analyze molecular diversity among different varieties of rice ... more The present study was performed to analyze molecular diversity among different varieties of rice using SSR markers, which are effective and reliable tools for this type of analysis. A total of 34 different alleles were generated using 17 SSR primers. Out of these 33 alleles were found to be polymorphic and only one was monomorphic. On average, 2 alleles per primer and 1.94 polymorphic alleles per primer was calculated. In the cluster analysis three varieties KATKIRICE, SONUMRICE and 1010 were found to be diverse from other varieties of rice. These varieties may be used as diverse parents for the future breeding programs for rice improvement. According to Jaccard’s similarity coefficient, the highest genetic diversity was observed between KATKIRICE and CHANDRAHASNI. Both varieties were grouped distantly. Out of 17 primers, the best 10 primers were selected based on polymorphic banding patterns for genetic diversity analysis. These selected 10 primers are sufficient to discriminate th...
International Journal of Plant & Soil Science
Four different concentrations of chitosan and four mungbean genotypes were studied to determine t... more Four different concentrations of chitosan and four mungbean genotypes were studied to determine the optimum concentration of chitosan seed priming. The experiment results revealed that germination percentage, shoot length, root length, fresh weight, dry weight, and seedling vigor index differed significantly among the treatments and genotypes. The greatest impact was observed at lower concentrations of 0.15% in all four genotypes showing consistent improvements in all the studied parameters. The highest concentration of 0.35% appears to be at par with the control and shows no statistical significance. However, root length was affected in IPM 2-14 and IPM 2-3 at high concentrations. In terms of seedling vigor index, IPM 2-3 showed significant reductions at 0.35%, while other genotypes recorded improvement. Based on our results, we conclude that chitosan seed priming has genotype-specific responses, and lower chitosan concentrations have significant positive effects on all parameters ...
International Journal of Plant & Soil Science
The growing human population has put enormous pressure on agriculture to increase production and ... more The growing human population has put enormous pressure on agriculture to increase production and productivity, which has resulted in the widespread usage of agrochemicals. The indiscriminate use of agrochemicals has harmed soil fertility and resulted in significant environmental contamination, impacting human health. The discovery and implementation of bacterial isolates with Plant Growth Promoting Rhizobacteria (PGPR) have enormous potential for reducing the usage of chemical fertilizers, insecticides, and herbicides. However, the identification of bacterial isolates is a prerequisite to utilize them for agricultural purposes. Traditional methods involve culturing microbes using a range of nonselective and selective enrichment methods, followed by biochemical confirmation among others. Traditional methods involve culturing microbes, followed by morphological biochemical confirmation etc. In the present investigation, we describe a fast and effective approach for isolating and ident...
Journal of Pharmaceutical Research International
COVID-19 (SARS-CoV-2) has recently emerged as an ongoing pandemic disease or probably syndemic an... more COVID-19 (SARS-CoV-2) has recently emerged as an ongoing pandemic disease or probably syndemic and became the latest threat to global health. The main route of transmission of virus droplet start with respiratory problems coughing, sneezing, fever during infection period, which may end up with death, if not treated. Many emerging epidemiological factors have made COVID-19 as global alarming disease. To control the outbreak of COVID-19, a critical management along with quarantine measure is applicable, which requires early detection of infection. The early detection of COVID-19 infection is playing a vital role in controlling of progression of illness and limiting viral spread within the population. Therefore, reliable, highly specific and sensitive diagnosis techniques are necessary for its early detection. Among several detection techniques, qRT-PCR is the rapidly employed, sensitive and widely accepted technique. In present review, different molecular techniques with emphasis on ...
Molecular Biology Reports
Ecology, Environment and Conservation
Rice-associated plant growth-promoting bacteria (PGPB) have a major role in promoting growth andd... more Rice-associated plant growth-promoting bacteria (PGPB) have a major role in promoting growth anddevelopment using a variety of mechanisms. In this study, we isolated bacteria from drought stressedfields to check their effects on rice plants. Bacterial strains were selectively isolated based on their growthon selective media and different biochemical tests. These bacteria were identified by partial sequencing of16s rDNA. We have found that two isolates, Brevibacterium sp. (B4) and Priestiaaryabhattai (B16) (MZ081422.1),have a magnificent role in the growth of rice plants compared to mock-treated plants after drought stresstreatment.
Current Journal of Applied Science and Technology
The genetic diversity was estimated using seventy two genotypes of rice in a randomized block des... more The genetic diversity was estimated using seventy two genotypes of rice in a randomized block design with three replications at the rice research farm of Bihar Agricultural University, Sabour (Bhagalpur) during Kharif, 2019-20 to determine the contribution of fifteen quantitative traits to the total variability in rice using Principal component analysis. In the present investigation PCA was performed for fifteen quantitative traits of rice. All the 3PCs exhibited more than 1.0 Eigen value and showed about 95.00% variability. Therefore, these PCs were given due important for the further explanation. The PC1 showed 77.28 per cent variation of total variation followed by second to third components which accounted 15.65 and 2.05 per cent of total variation presented among the genotypes, respectively. PC1 contributed 77.28% of the total variation and correlated with total carbohydrate, generation of H2O2, days to 50% flowering, biological yield, number of fertile grains per panicle, pani...
International Journal of Plant & Soil Science
The current work sought to investigate the impact of two chemicals (NaCl and Brassinolide) on gro... more The current work sought to investigate the impact of two chemicals (NaCl and Brassinolide) on growth parameters, chlorophyll pigments in stevia shoots cultured under in vitro condition. For this, nodal explants of stevia were cultured for four weeks on MS medium supplemented with 0.2 mg l-1 BAP, Kinetin 0.2 mg l-1 and various concentrations of NaCl (0, 60, 70, 90, 120, 150 mM) and BL (0, 1, 2, 3 4, 5µM) for screening under in vitro condition. Explant treated with NaCl showed reduction with increasing salinity levels in all the studied parameters while explants treated with Brassinolide showed best results in all the studied parameters. From the results, it is concluded that the stevia plant was less impacted by 60mM and 70mM NaCl concentrations when compared to other concentrations of NaCl, but BL increased/improved all the examined parameters when compared to control; 1µM and 2µM concentrations of BL responded the best.
Biopesticides and Bioagents, 2016
BackgroundOccurrence and prevention of diseases have a big impact on sustainability of staple foo... more BackgroundOccurrence and prevention of diseases have a big impact on sustainability of staple food crop like rice. The crosstalk between phytohormones and secondary messengers in host cell during infestation of pathogens play a pivotal role in defence responses. Apart from SA and JA, the role of brassinosteroids (BR) in defence responses in plants is unprecedented. The calcium signatures observed during early infection process modulates the expression of calmodulin and other Ca2+ binding protein followed by their interactions with calmodulin binding protein (CBP), which are pivotal in elucidating defence responses in plant. Numerous CBPs have been identified, which modulated stress responses with the help of CBD and other functional domain. Interestingly, Arabidopsis CBP60 (AtCBP60) family protein, SARD1, was involved in defense responses via SAR. However, no rice CBP60 (OsCBP60) has been identified in relation to pathogen infection yet. ResultsIn present investigation, 15 OsCBP60 g...
Biofertilizers and Biopesticides in Sustainable Agriculture, 2019
Australasian Plant Pathology, 2022
![Research paper thumbnail of Salicylic Acid Induced Resistance against Mungbean Yellow Mosaic Virus (MYMV) and Enhanced Seed Yield in Resistant and Susceptible Urdbean [Vigna mungo (L.) Heper] Genotypes](https://attachments.academia-assets.com/93937155/thumbnails/1.jpg)
](LEGUME RESEARCH - AN INTERNATIONAL JOURNAL, 2021
Background: Urdbean’s low productivity is largely due to its susceptibility against whitefly-tran... more Background: Urdbean’s low productivity is largely due to its susceptibility against whitefly-transmitted mungbean yellow mosaic virus (MYMV) disease. The effect of Salicylic acid (SA) on MYMV disease resistance and its impact on seed yield under field conditions on diverse genotypes is largely unknown. Therefore, in present investigation, we have analysed the effect of SA on induction of antioxidant enzymes leading to MYMV resistance and enhanced seed yield in urdbean genotypes. Methods: Different concentrations of SA were sprayed on 3 week-old susceptible urdbean genotype (LBG 623) and induction of antioxidant enzymes was analysed. A pot experiment was conducted to see the effect of SA on initial induction of antioxidant enzymes maintained over long period of time in 39 urdbean genotypes. Under field conditions, the effect of SA treatment on MYMV disease resistance and seed yield was assessed. Result: Rise in antioxidant enzyme production was observed in SA treated urdbean plants c...
International Journal of Current Microbiology and Applied Sciences, 2020
Red rot of sugarcane caused by Colletotrichum falcatum is the major devastating disease of sugarc... more Red rot of sugarcane caused by Colletotrichum falcatum is the major devastating disease of sugarcane. This disease causes losses in both sugarcane yield and sugar recovery. Most commonly affected areas of India are Bihar, Punjab and Uttar Pradesh but now it spreads to coastal areas of Tamil Nadu and Gujarat. The primary aim of this study was to isolate Bacillus strains from different climatic condition and check the antifungal activity against three different strains of Colletotrichum falcatum isolated from affected sugarcane grown in South Gujarat. A total of 45 Bacillus strains were isolated from different samples. Seven out of these isolates showed significant inhibition against all the three strains of the pathogen Colletotrichum falcatum (i.e. CFNAV, CFTIM and CFKAM). One from these seven isolates, the Bacillus spp. strain AV5 showed the maximum inhibition (percentage) of the three fungus strains (i.e. 60, 58 and 55 respectively) suggesting its potential use as bio-control agen...
International Journal of Plant & Soil Science
The present study was performed to analyze molecular diversity among different varieties of rice ... more The present study was performed to analyze molecular diversity among different varieties of rice using SSR markers, which are effective and reliable tools for this type of analysis. A total of 34 different alleles were generated using 17 SSR primers. Out of these 33 alleles were found to be polymorphic and only one was monomorphic. On average, 2 alleles per primer and 1.94 polymorphic alleles per primer was calculated. In the cluster analysis three varieties KATKIRICE, SONUMRICE and 1010 were found to be diverse from other varieties of rice. These varieties may be used as diverse parents for the future breeding programs for rice improvement. According to Jaccard’s similarity coefficient, the highest genetic diversity was observed between KATKIRICE and CHANDRAHASNI. Both varieties were grouped distantly. Out of 17 primers, the best 10 primers were selected based on polymorphic banding patterns for genetic diversity analysis. These selected 10 primers are sufficient to discriminate th...
International Journal of Plant & Soil Science
Four different concentrations of chitosan and four mungbean genotypes were studied to determine t... more Four different concentrations of chitosan and four mungbean genotypes were studied to determine the optimum concentration of chitosan seed priming. The experiment results revealed that germination percentage, shoot length, root length, fresh weight, dry weight, and seedling vigor index differed significantly among the treatments and genotypes. The greatest impact was observed at lower concentrations of 0.15% in all four genotypes showing consistent improvements in all the studied parameters. The highest concentration of 0.35% appears to be at par with the control and shows no statistical significance. However, root length was affected in IPM 2-14 and IPM 2-3 at high concentrations. In terms of seedling vigor index, IPM 2-3 showed significant reductions at 0.35%, while other genotypes recorded improvement. Based on our results, we conclude that chitosan seed priming has genotype-specific responses, and lower chitosan concentrations have significant positive effects on all parameters ...
International Journal of Plant & Soil Science
The growing human population has put enormous pressure on agriculture to increase production and ... more The growing human population has put enormous pressure on agriculture to increase production and productivity, which has resulted in the widespread usage of agrochemicals. The indiscriminate use of agrochemicals has harmed soil fertility and resulted in significant environmental contamination, impacting human health. The discovery and implementation of bacterial isolates with Plant Growth Promoting Rhizobacteria (PGPR) have enormous potential for reducing the usage of chemical fertilizers, insecticides, and herbicides. However, the identification of bacterial isolates is a prerequisite to utilize them for agricultural purposes. Traditional methods involve culturing microbes using a range of nonselective and selective enrichment methods, followed by biochemical confirmation among others. Traditional methods involve culturing microbes, followed by morphological biochemical confirmation etc. In the present investigation, we describe a fast and effective approach for isolating and ident...
Journal of Pharmaceutical Research International
COVID-19 (SARS-CoV-2) has recently emerged as an ongoing pandemic disease or probably syndemic an... more COVID-19 (SARS-CoV-2) has recently emerged as an ongoing pandemic disease or probably syndemic and became the latest threat to global health. The main route of transmission of virus droplet start with respiratory problems coughing, sneezing, fever during infection period, which may end up with death, if not treated. Many emerging epidemiological factors have made COVID-19 as global alarming disease. To control the outbreak of COVID-19, a critical management along with quarantine measure is applicable, which requires early detection of infection. The early detection of COVID-19 infection is playing a vital role in controlling of progression of illness and limiting viral spread within the population. Therefore, reliable, highly specific and sensitive diagnosis techniques are necessary for its early detection. Among several detection techniques, qRT-PCR is the rapidly employed, sensitive and widely accepted technique. In present review, different molecular techniques with emphasis on ...
Molecular Biology Reports
Ecology, Environment and Conservation
Rice-associated plant growth-promoting bacteria (PGPB) have a major role in promoting growth andd... more Rice-associated plant growth-promoting bacteria (PGPB) have a major role in promoting growth anddevelopment using a variety of mechanisms. In this study, we isolated bacteria from drought stressedfields to check their effects on rice plants. Bacterial strains were selectively isolated based on their growthon selective media and different biochemical tests. These bacteria were identified by partial sequencing of16s rDNA. We have found that two isolates, Brevibacterium sp. (B4) and Priestiaaryabhattai (B16) (MZ081422.1),have a magnificent role in the growth of rice plants compared to mock-treated plants after drought stresstreatment.
Current Journal of Applied Science and Technology
The genetic diversity was estimated using seventy two genotypes of rice in a randomized block des... more The genetic diversity was estimated using seventy two genotypes of rice in a randomized block design with three replications at the rice research farm of Bihar Agricultural University, Sabour (Bhagalpur) during Kharif, 2019-20 to determine the contribution of fifteen quantitative traits to the total variability in rice using Principal component analysis. In the present investigation PCA was performed for fifteen quantitative traits of rice. All the 3PCs exhibited more than 1.0 Eigen value and showed about 95.00% variability. Therefore, these PCs were given due important for the further explanation. The PC1 showed 77.28 per cent variation of total variation followed by second to third components which accounted 15.65 and 2.05 per cent of total variation presented among the genotypes, respectively. PC1 contributed 77.28% of the total variation and correlated with total carbohydrate, generation of H2O2, days to 50% flowering, biological yield, number of fertile grains per panicle, pani...
International Journal of Plant & Soil Science
The current work sought to investigate the impact of two chemicals (NaCl and Brassinolide) on gro... more The current work sought to investigate the impact of two chemicals (NaCl and Brassinolide) on growth parameters, chlorophyll pigments in stevia shoots cultured under in vitro condition. For this, nodal explants of stevia were cultured for four weeks on MS medium supplemented with 0.2 mg l-1 BAP, Kinetin 0.2 mg l-1 and various concentrations of NaCl (0, 60, 70, 90, 120, 150 mM) and BL (0, 1, 2, 3 4, 5µM) for screening under in vitro condition. Explant treated with NaCl showed reduction with increasing salinity levels in all the studied parameters while explants treated with Brassinolide showed best results in all the studied parameters. From the results, it is concluded that the stevia plant was less impacted by 60mM and 70mM NaCl concentrations when compared to other concentrations of NaCl, but BL increased/improved all the examined parameters when compared to control; 1µM and 2µM concentrations of BL responded the best.
Biopesticides and Bioagents, 2016
BackgroundOccurrence and prevention of diseases have a big impact on sustainability of staple foo... more BackgroundOccurrence and prevention of diseases have a big impact on sustainability of staple food crop like rice. The crosstalk between phytohormones and secondary messengers in host cell during infestation of pathogens play a pivotal role in defence responses. Apart from SA and JA, the role of brassinosteroids (BR) in defence responses in plants is unprecedented. The calcium signatures observed during early infection process modulates the expression of calmodulin and other Ca2+ binding protein followed by their interactions with calmodulin binding protein (CBP), which are pivotal in elucidating defence responses in plant. Numerous CBPs have been identified, which modulated stress responses with the help of CBD and other functional domain. Interestingly, Arabidopsis CBP60 (AtCBP60) family protein, SARD1, was involved in defense responses via SAR. However, no rice CBP60 (OsCBP60) has been identified in relation to pathogen infection yet. ResultsIn present investigation, 15 OsCBP60 g...
Biofertilizers and Biopesticides in Sustainable Agriculture, 2019
Australasian Plant Pathology, 2022
LEGUME RESEARCH - AN INTERNATIONAL JOURNAL, 2021
Background: Urdbean’s low productivity is largely due to its susceptibility against whitefly-tran... more Background: Urdbean’s low productivity is largely due to its susceptibility against whitefly-transmitted mungbean yellow mosaic virus (MYMV) disease. The effect of Salicylic acid (SA) on MYMV disease resistance and its impact on seed yield under field conditions on diverse genotypes is largely unknown. Therefore, in present investigation, we have analysed the effect of SA on induction of antioxidant enzymes leading to MYMV resistance and enhanced seed yield in urdbean genotypes. Methods: Different concentrations of SA were sprayed on 3 week-old susceptible urdbean genotype (LBG 623) and induction of antioxidant enzymes was analysed. A pot experiment was conducted to see the effect of SA on initial induction of antioxidant enzymes maintained over long period of time in 39 urdbean genotypes. Under field conditions, the effect of SA treatment on MYMV disease resistance and seed yield was assessed. Result: Rise in antioxidant enzyme production was observed in SA treated urdbean plants c...
International Journal of Current Microbiology and Applied Sciences, 2020
Red rot of sugarcane caused by Colletotrichum falcatum is the major devastating disease of sugarc... more Red rot of sugarcane caused by Colletotrichum falcatum is the major devastating disease of sugarcane. This disease causes losses in both sugarcane yield and sugar recovery. Most commonly affected areas of India are Bihar, Punjab and Uttar Pradesh but now it spreads to coastal areas of Tamil Nadu and Gujarat. The primary aim of this study was to isolate Bacillus strains from different climatic condition and check the antifungal activity against three different strains of Colletotrichum falcatum isolated from affected sugarcane grown in South Gujarat. A total of 45 Bacillus strains were isolated from different samples. Seven out of these isolates showed significant inhibition against all the three strains of the pathogen Colletotrichum falcatum (i.e. CFNAV, CFTIM and CFKAM). One from these seven isolates, the Bacillus spp. strain AV5 showed the maximum inhibition (percentage) of the three fungus strains (i.e. 60, 58 and 55 respectively) suggesting its potential use as bio-control agen...