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Research paper thumbnail of Three new beetle-associated yeast species in the clade

Fems Yeast Res, 2004

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Research paper thumbnail of Incidence, Host Specificity, Distribution, and Morphological Variation in Arthrorhynchus nycteribiae and A. eucampsipodae (Laboulbeniomycetes)

Mycologia, 1980

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Research paper thumbnail of A New Genus in the Lophiaceae

Mycologia, 1980

... Mycologia 69: 1162-1172. Singer, R. 1975. The Agaricales in modern taxonomy, 3rd ed. Cramer, ... more ... Mycologia 69: 1162-1172. Singer, R. 1975. The Agaricales in modern taxonomy, 3rd ed. Cramer, Vaduz. 912 p. A NEW GENUS IN THE LOPHIACEAE ... Mycologia 69: 1162-1172. Singer, R. 1975. The Agaricales in modern taxonomy, 3rd ed. Cramer, Vaduz. 912 p. ...

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Research paper thumbnail of Fungi associated with African earwigs and their relationship to South American forms

Mycologia, 1990

... AND Meredith Blackwell Department of Botany, Louisiana State University, Baton Rouge, Louisia... more ... AND Meredith Blackwell Department of Botany, Louisiana State University, Baton Rouge, Louisiana 70803 ... Key Words: Ear wigs, entomogenous fungi, Africa LITERATURE CITED Blackwell, M., JR Bridges, JC Moser, and TJ Perry. 1986a. Hyperphoretic dispersal ofa Pyx? ...

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Research paper thumbnail of Fungal biotrophic parasites of insects and other arthropods

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Research paper thumbnail of Population Structure of Dogwood Anthracnose Fungus

Phytopathology, 2002

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Research paper thumbnail of Extreme morphological divergence: phylogenetic position of a termite ectoparasite

Species of Termitaria are lesion-forming ectoparasites occurring worldwide on a diverse group of ... more Species of Termitaria are lesion-forming ectoparasites occurring worldwide on a diverse group of termites. The reduced thallus consists of a basal cell layer from which haustorial cells penetrate the termite and a darkly pigmented sporodochium. One species, Termitaria snyderi, has been the subject of several morphological studies, but its phylogenetic position has remained enigmatic. Here we provide evidence of a

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Research paper thumbnail of Phylogeography of Metarhizium, an insect pathogenic fungus

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Research paper thumbnail of Caps off to the BMS

Mycologist, 1997

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Research paper thumbnail of Ecology and evolution of mycophagous bark beetles and their fungal partners

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Research paper thumbnail of Three new beetle-associated yeast species in the Pichia guilliermondii clade

Fems Yeast Research, Oct 1, 2004

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Research paper thumbnail of Molecular Phylogeny of the Cleistothecial Fungi Placed in Cephalothecaceaeand Pseudeurotiaceae

Mycologia, 1999

... Sung-Oui Suhl Meredith Blackwell Department of Biological Sciences, Louisiana State Universit... more ... Sung-Oui Suhl Meredith Blackwell Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803 ... of Hypocreales, Sordariales, Ophiostomatales, and Mi-croascales have been recognized (Benny and Kim-brough 1980, de Hoog 1974, Malloch ...

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Research paper thumbnail of Trends and impact of host utilization in beetles

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Research paper thumbnail of gen. and sp. nov., a unique cuticular fungus infecting Japanese beetle, (Coleoptera: Scarabaeidae)

J Invertebr Pathol, 1991

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Research paper thumbnail of Phylogenetic distribution of fungal sterols

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Research paper thumbnail of Ultrastructure of Orbilia auricolor and the phylogeny of Pezizomycotina

Molecular phylogenetic analyses indicate that the monophyletic classes Orbiliomycetes and Pezizom... more Molecular phylogenetic analyses indicate that the monophyletic classes Orbiliomycetes and Pezizomycetes are among the earliest diverging branches of Pezizomycotina, the largest subphylum of the Ascomycota. Although Orbiliomycetes is resolved as the most basal lineage in some analyses, molecular support for the node resolving the relationships between the two classes is low and topologies are unstable. We provide ultrastructural evidence to inform the placement of Orbiliomycetes by studying Orbilia auricolor, a representative species of the only order (Orbiliales) of the class. The truncate ascus apex in O. auricolor is thin-walled except at the margin, and an irregular wall rupture of the apex permits ascospore discharge. Ascus, ascogenous and non-ascogenous hyphae were simple septate, with septal pores plugged by unelaborated electrondense, non-membranous occlusions. Globose Woronin bodies were located on both sides of the septum. Nuclear division was characterized by the retention...

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Research paper thumbnail of Cladistic Analysis of Partial ssrDNA Sequences among Unitunicate Perithecial Ascomycetes and Its Implications on the Evolution of Centrum Development

Ascomycete Systematics, 1994

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Research paper thumbnail of Molecular Systematics of Unitunicate Perithecial Ascomycetes: The Clavicipitales-Hypocreales Connection

Mycologia, 1993

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Research paper thumbnail of Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA

BioTechniques, 1992

A modification of the asymmetric PCR method is described, which reliably facilitates sequencing o... more A modification of the asymmetric PCR method is described, which reliably facilitates sequencing of PCR-amplified DNA. This procedure produces single-stranded DNA fragments as long as two kilobases that are suitable for dideoxy DNA sequencing. First, a PCR for double-stranded DNA is preformed under optimal conditions (double-stranded PCR). Then, a 5-10-microliters fraction of the double-stranded PCR and a single primer are used to generate single-stranded DNA in a separate PCR (single-stranded PCR). The concentration of the single primer are used to generate single-stranded DNA in a separate PCR (single-stranded PCR). The concentration of the single primer is approximately 0.4 microM. Usually 15 to 25 cycles of single-stranded PCR are optimal to produce single-stranded DNA for four to eight sequencing reactions. The single-stranded DNA is purified by centrifugal ultrafiltration and used directly in dideoxy sequencing. This method was employed to produce high-quality single-stranded D...

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Research paper thumbnail of The status and characterization of Enteroramus dimorphus: a xylose-fermenting yeast attached to the gut of beetles

Mycologia

Enteroramus dimorphus from the gut of the passalid beetle Odontotaenius disjunctus was described ... more Enteroramus dimorphus from the gut of the passalid beetle Odontotaenius disjunctus was described originally as a yeast-like fungus of unknown taxonomic affiliation. The fungus can be observed in situ, attached by a specialized cell to the beetle hindgut wall. In a recent study of yeast endosymbionts from a variety of beetles, we discovered that E. dimorphus is a member of the Pichia stipitis (Saccharomycetes) clade, known for xylose fermentation and assimilation. The closest relative of E. dimorphus is the PASS1 isolate, repeatedly acquired from passalid beetles in eastern North America from Pennsylvania to Louisiana. In addition to xylose fermentation and assimilation, these yeasts are characterized by the production of hat-shaped ascospores in culture, assimilation of a wide range of sugars, and synthesis of several vitamins. Enteroramus dimorphus, however, can be distinguished from close relatives by several physiological characteristics and rDNA sequences, which vary slightly fr...

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Research paper thumbnail of Three new beetle-associated yeast species in the clade

Fems Yeast Res, 2004

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Research paper thumbnail of Incidence, Host Specificity, Distribution, and Morphological Variation in Arthrorhynchus nycteribiae and A. eucampsipodae (Laboulbeniomycetes)

Mycologia, 1980

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Research paper thumbnail of A New Genus in the Lophiaceae

Mycologia, 1980

... Mycologia 69: 1162-1172. Singer, R. 1975. The Agaricales in modern taxonomy, 3rd ed. Cramer, ... more ... Mycologia 69: 1162-1172. Singer, R. 1975. The Agaricales in modern taxonomy, 3rd ed. Cramer, Vaduz. 912 p. A NEW GENUS IN THE LOPHIACEAE ... Mycologia 69: 1162-1172. Singer, R. 1975. The Agaricales in modern taxonomy, 3rd ed. Cramer, Vaduz. 912 p. ...

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Research paper thumbnail of Fungi associated with African earwigs and their relationship to South American forms

Mycologia, 1990

... AND Meredith Blackwell Department of Botany, Louisiana State University, Baton Rouge, Louisia... more ... AND Meredith Blackwell Department of Botany, Louisiana State University, Baton Rouge, Louisiana 70803 ... Key Words: Ear wigs, entomogenous fungi, Africa LITERATURE CITED Blackwell, M., JR Bridges, JC Moser, and TJ Perry. 1986a. Hyperphoretic dispersal ofa Pyx? ...

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Research paper thumbnail of Fungal biotrophic parasites of insects and other arthropods

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Population Structure of Dogwood Anthracnose Fungus

Phytopathology, 2002

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Research paper thumbnail of Extreme morphological divergence: phylogenetic position of a termite ectoparasite

Species of Termitaria are lesion-forming ectoparasites occurring worldwide on a diverse group of ... more Species of Termitaria are lesion-forming ectoparasites occurring worldwide on a diverse group of termites. The reduced thallus consists of a basal cell layer from which haustorial cells penetrate the termite and a darkly pigmented sporodochium. One species, Termitaria snyderi, has been the subject of several morphological studies, but its phylogenetic position has remained enigmatic. Here we provide evidence of a

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Research paper thumbnail of Phylogeography of Metarhizium, an insect pathogenic fungus

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Research paper thumbnail of Caps off to the BMS

Mycologist, 1997

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Research paper thumbnail of Ecology and evolution of mycophagous bark beetles and their fungal partners

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Three new beetle-associated yeast species in the Pichia guilliermondii clade

Fems Yeast Research, Oct 1, 2004

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Research paper thumbnail of Molecular Phylogeny of the Cleistothecial Fungi Placed in Cephalothecaceaeand Pseudeurotiaceae

Mycologia, 1999

... Sung-Oui Suhl Meredith Blackwell Department of Biological Sciences, Louisiana State Universit... more ... Sung-Oui Suhl Meredith Blackwell Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803 ... of Hypocreales, Sordariales, Ophiostomatales, and Mi-croascales have been recognized (Benny and Kim-brough 1980, de Hoog 1974, Malloch ...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Trends and impact of host utilization in beetles

Bookmarks Related papers MentionsView impact

Research paper thumbnail of gen. and sp. nov., a unique cuticular fungus infecting Japanese beetle, (Coleoptera: Scarabaeidae)

J Invertebr Pathol, 1991

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Phylogenetic distribution of fungal sterols

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Ultrastructure of Orbilia auricolor and the phylogeny of Pezizomycotina

Molecular phylogenetic analyses indicate that the monophyletic classes Orbiliomycetes and Pezizom... more Molecular phylogenetic analyses indicate that the monophyletic classes Orbiliomycetes and Pezizomycetes are among the earliest diverging branches of Pezizomycotina, the largest subphylum of the Ascomycota. Although Orbiliomycetes is resolved as the most basal lineage in some analyses, molecular support for the node resolving the relationships between the two classes is low and topologies are unstable. We provide ultrastructural evidence to inform the placement of Orbiliomycetes by studying Orbilia auricolor, a representative species of the only order (Orbiliales) of the class. The truncate ascus apex in O. auricolor is thin-walled except at the margin, and an irregular wall rupture of the apex permits ascospore discharge. Ascus, ascogenous and non-ascogenous hyphae were simple septate, with septal pores plugged by unelaborated electrondense, non-membranous occlusions. Globose Woronin bodies were located on both sides of the septum. Nuclear division was characterized by the retention...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Cladistic Analysis of Partial ssrDNA Sequences among Unitunicate Perithecial Ascomycetes and Its Implications on the Evolution of Centrum Development

Ascomycete Systematics, 1994

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular Systematics of Unitunicate Perithecial Ascomycetes: The Clavicipitales-Hypocreales Connection

Mycologia, 1993

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA

BioTechniques, 1992

A modification of the asymmetric PCR method is described, which reliably facilitates sequencing o... more A modification of the asymmetric PCR method is described, which reliably facilitates sequencing of PCR-amplified DNA. This procedure produces single-stranded DNA fragments as long as two kilobases that are suitable for dideoxy DNA sequencing. First, a PCR for double-stranded DNA is preformed under optimal conditions (double-stranded PCR). Then, a 5-10-microliters fraction of the double-stranded PCR and a single primer are used to generate single-stranded DNA in a separate PCR (single-stranded PCR). The concentration of the single primer are used to generate single-stranded DNA in a separate PCR (single-stranded PCR). The concentration of the single primer is approximately 0.4 microM. Usually 15 to 25 cycles of single-stranded PCR are optimal to produce single-stranded DNA for four to eight sequencing reactions. The single-stranded DNA is purified by centrifugal ultrafiltration and used directly in dideoxy sequencing. This method was employed to produce high-quality single-stranded D...

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Research paper thumbnail of The status and characterization of Enteroramus dimorphus: a xylose-fermenting yeast attached to the gut of beetles

Mycologia

Enteroramus dimorphus from the gut of the passalid beetle Odontotaenius disjunctus was described ... more Enteroramus dimorphus from the gut of the passalid beetle Odontotaenius disjunctus was described originally as a yeast-like fungus of unknown taxonomic affiliation. The fungus can be observed in situ, attached by a specialized cell to the beetle hindgut wall. In a recent study of yeast endosymbionts from a variety of beetles, we discovered that E. dimorphus is a member of the Pichia stipitis (Saccharomycetes) clade, known for xylose fermentation and assimilation. The closest relative of E. dimorphus is the PASS1 isolate, repeatedly acquired from passalid beetles in eastern North America from Pennsylvania to Louisiana. In addition to xylose fermentation and assimilation, these yeasts are characterized by the production of hat-shaped ascospores in culture, assimilation of a wide range of sugars, and synthesis of several vitamins. Enteroramus dimorphus, however, can be distinguished from close relatives by several physiological characteristics and rDNA sequences, which vary slightly fr...

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