Branislav Ruttkay-nedecky - Academia.edu (original) (raw)

Papers by Branislav Ruttkay-nedecky

NANOCON 2019 Conference Proeedings

The AgNPs have considerable industrial potential and are intensively studied with regard to their... more The AgNPs have considerable industrial potential and are intensively studied with regard to their antibacterial properties. Using green synthesis, the nanoparticle surface can be coated with molecules that exhibit biologically significant properties. Increased use of nanoparticles enhances the risk of their release into the environment. However, there is still little known about the behaviour of AgNPs in the eco-environment. In this study, the effect of AgNPs prepared by green synthesis on germinated plants of maize was investigated. The effects on germination, basic growth and physiological parameters of the plant were monitored. AgNPs and silver nitrate were applied to maize seedlings (5 days in hydroponics system) at concentrations of 1 mg/L, 50 mg/L, and 150 mg/L. Chlorophyll a and b content was determined. We found that the growth inhibition of the above-ground parts of plants was about 40 % and AgNPs and silver nitrate had a significant effect on photosynthetic pigments. Chlorophyll a and b content changed with time and concentration.

International Journal of Electrochemical Science, Apr 1, 2013

Electrochemical methods are a reliable tool for a fast and low cost assay of phenolic compounds (... more Electrochemical methods are a reliable tool for a fast and low cost assay of phenolic compounds (phenolics) in food samples. The methods are precise and sesnitive enough to assay low content of polyphenols. The devices can be stationary or flow through, and based on voltammetry or amperometry. The application of voltammetric methods and HPLC-ED methods for analysis of phenolics in food samples is described in this review. The advantages and disadvantages of the methods are discussed.

Nanomaterials, Oct 31, 2019

AgNPs have attracted considerable attention in many applications including industrial use, and th... more AgNPs have attracted considerable attention in many applications including industrial use, and their antibacterial properties have been widely investigated. Due to the green synthesis process employed, the nanoparticle surface can be coated with molecules with biologically important characteristics. It has been reported that increased use of nanoparticles elevates the risk of their release into the environment. However, little is known about the behaviour of AgNPs in the eco-environment. In this study, the effect of green synthesized AgNPs on germinated plants of maize was examined. The effects on germination, basic growth and physiological parameters of the plants were monitored. Moreover, the effect of AgNPs was compared with that of Ag(I) ions in the form of AgNO 3 solution. It was found that the growth inhibition of the above-ground parts of plants was about 40%, and AgNPs exhibited a significant effect on photosynthetic pigments. Significant differences in the following parameters were observed: weights of the caryopses and fresh weight (FW) of primary roots after 96 h of exposure to Ag(I) ions and AgNPs compared to the control and between Ag compounds. In addition, the coefficient of velocity of germination (CVG) between the control and the AgNPs varied and that between the Ag(I) ions and AgNPs was also different. Phytotoxicity was proved in the following sequence: control < AgNPs < Ag(I) ions.

NANOCON 2019 Conference Proeedings, 2020

In this work, we focused on the green synthesis of silver nanoparticles (AgNPs). In the green syn... more In this work, we focused on the green synthesis of silver nanoparticles (AgNPs). In the green synthesis, plant extracts are used as reducing agents. AgNPs contain surface-bound biomolecules from the used plant extract. We used an aqueous extract from sage (Salvia officinalis). This plant is known for its high content of secondary metabolites with excellent antibacterial activity. Antibacterial activity of AgNPs prepared by green synthesis was investigated. Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli were selected for testing. Methods such as agar diffusion assay and inhibition of bacterial growth curves were used. Minimum inhibitory concentrations (MICE. coli = 250 µg/mL; MICS. aureus = 50 µg/mL) were determined. The radiuses of inhibition zones were about 1.5 cm in both bacterial strains. AgNPs prepared by this method can play an important role in dealing with the problem of bacterial resistance. Bacteria develop resistance not only to typical antibiotics. Bacterial cells are also able to eliminate the effect of AgNPs. By modifying their surface with antibacterial molecules, resistance can be effectively combatted.

NANOCON 2019 Conference Proeedings, 2020

3D printing is a modern and widely used technology that has reached virtually all sectors of huma... more 3D printing is a modern and widely used technology that has reached virtually all sectors of human activity, from the automotive industry to medical fields. Silver nanoparticles (AgNPs) have considerable industrial potential and are intensively studied regarding their antibacterial capability. Using green synthesis, the nanoparticle surface can be coated with molecules that exhibit biologically significant properties. By combining 3D printing technology and antibacterial activity of AgNPs, a unique antibacterial material can be prepared. The aim of this work was to modify the printing filament with AgNPs, to prepare the antibacterial material by 3D printing and to test the antibacterial activity of the prepared material using the JIS (Japanese industrial standards) L 1902 method. Such material can be used in hospital facilities to adjust various devices to prevent nosocomial infections.

We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/... more We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/VIS spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified fragment of DNA of 498 bp. DNA was subsequently incubated with Zn(II) ions for 60 min at 25 C. After incubation, samples were dialyzed and analysed immediately. In this way, DNA was converted into a metal bound DNA (M-DNA). Interaction of Zn(II) with DNA showed a change of the absorption spectrum (190-350 nm) and a decrease in the M-DNA melting temperature. Spectrophotometric (UV/VIS) analysis showed that increasing concentrations of Zn(II) led to an increase in absorbance at 200 nm and a decrease in absorbance at 260 nm. Application of 5.5 microM Zn(II) caused a decrease in the melting temperature (Tm) for about 7.5 C in average in comparison with control (75.5 +- 3 C). The lowest melting temperature (60.5 +- 2.5 C) was observed after application of 33 microM Zn(II). On the other hand, application of two c...

Nanomaterials, 2020

The irradiance of ultraviolet (UV) radiation is a physical parameter that significantly influence... more The irradiance of ultraviolet (UV) radiation is a physical parameter that significantly influences biological molecules by affecting their molecular structure. The influence of UV radiation on nanoparticles has not been investigated much. In this work, the ability of cadmium telluride quantum dots (CdTe QDs) to respond to natural UV radiation was examined. The average size of the yellow QDs was 4 nm, and the sizes of green, red and orange QDs were 2 nm. Quantum yield of green CdTe QDs-MSA (mercaptosuccinic acid)-A, yellow CdTe QDs-MSA-B, orange CdTe QDs-MSA-C and red CdTe QDs-MSA-D were 23.0%, 16.0%, 18.0% and 7.0%, respectively. Green, yellow, orange and red CdTe QDs were replaced every day and exposed to daily UV radiation for 12 h for seven consecutive days in summer with UV index signal integration ranging from 1894 to 2970. The rising dose of UV radiation led to the release of cadmium ions and the change in the size of individual QDs. The shifts were evident in absorption signa...

International Journal of Electrochemical Science

Electrochemical methods are a reliable tool for a fast and low cost assay of phenolic compounds (... more Electrochemical methods are a reliable tool for a fast and low cost assay of phenolic compounds (phenolics) in food samples. The methods are precise and sesnitive enough to assay low content of polyphenols. The devices can be stationary or flow through, and based on voltammetry or amperometry. The application of voltammetric methods and HPLC-ED methods for analysis of phenolics in food samples is described in this review. The advantages and disadvantages of the methods are discussed.

Lipid Peroxidation, 2012

Lipid Peroxidation 132 with another molecule of fatty acid, from which a hydrogen atom is detache... more Lipid Peroxidation 132 with another molecule of fatty acid, from which a hydrogen atom is detached under formation of lipid hydroperoxide from original molecule (3). After pairing of all radicals, the last stage of the reaction called termination occurs. In addition to the above-mentioned chemical non-enzymatic peroxidation, enzymatic lipid peroxidation that is catalysed by the enzymes cyclooxygenase and lipoxygenase takes place. [26,28]. Both enzymes are involved in the formation of eicosanoids, which represent a group of biologically active lipid compounds derived from unsaturated fatty acids containing 20 carbon atoms. Cyclooxygenase is involved in the genesis of prostaglandins [29].

Journal of nanobiotechnology, Jan 26, 2017

Nanomaterials in agriculture are becoming popular due to the impressive advantages of these parti... more Nanomaterials in agriculture are becoming popular due to the impressive advantages of these particles. However, their bioavailability and toxicity are key features for their massive employment. Herein, we comprehensively summarize the latest findings on the phytotoxicity of nanomaterial products based on essential metals used in plant protection. The metal nanoparticles (NPs) synthesized from essential metals belong to the most commonly manufactured types of nanomaterials since they have unique physical and chemical properties and are used in agricultural and biotechnological applications, which are discussed. The paper discusses the interactions of nanomaterials and vascular plants, which are the subject of intensive research because plants closely interact with soil, water, and atmosphere; they are also part of the food chain. Regarding the accumulation of NPs in the plant body, their quantification and localization is still very unclear and further research in this area is necess...

Abstract: Polymers are currently widely used to replace a variety of natural materials with respe... more Abstract: Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of application of polymers is the production of different products for medical use. In this case, it is necessary to face a significant disadvantage of polymer products due to possible and very common colonization of the surface by various microorganisms that can pose a potential danger to the patient. One of the possible solutions is to prepare polymer with antibacterial/antimicrobial properties that is resistant to bacterial colonization. The aim of this study was to contribute to the development of antimicrobial polymeric material ideal for covering vascular implants with subsequent use in transplant surgery. Therefore, the complexes of polymeric substances (hyaluronic acid and chitosan) with silver nitrate orInt. J. Mol. Sci. 2013, 14 13593

In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(hi... more In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(his)Cl 2 , Zn(his)Cl 2) and ZnS quantum dots (QDs) using printed electrodes. This method was chosen due to the simple (easy to use) instrumentation and variable setting of flows. Reduction signals of zinc under the strictly defined and controlled conditions (pH, temperature, flow rate, accumulation time and applied potential) were studied. We showed that the increasing concentration of the complexes (Zn(phen)(his)Cl 2 , Zn(his)Cl 2) led to a decrease in the electrochemical signal and a significant shift of the potential to more positive values. The most likely explanation of this result is that zinc is strongly bound in the complex and its distribution on the electrode is very limited. Changing the pH from 3.5 to 5.5 resulted in a significant intensification of the Zn(II)

1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemed... more 1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, European Union 2 Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ616 00 Brno, Czech Republic, European Union 3 Vysoka skola Karla Englise, Sujanovo square 356/1, CZ-602 00, Brno, Czech Republic, European Union * E-mail: vojtech.adam@mendelu.cz

Pharmaceutics, 2020

Silver nanoparticles (AgNPs) have recently become very attractive for the scientific community du... more Silver nanoparticles (AgNPs) have recently become very attractive for the scientific community due to their broad spectrum of applications in the biomedical field. The main advantages of AgNPs include a simple method of synthesis, a simple way to change their morphology and high surface area to volume ratio. Much research has been carried out over the years to evaluate their possible effectivity against microbial organisms. The most important factors which influence the effectivity of AgNPs against microorganisms are the method of their preparation and the type of application. When incorporated into fabric wound dressings and other textiles, AgNPs have shown significant antibacterial activity against both Gram-positive and Gram-negative bacteria and inhibited biofilm formation. In this review, the different routes of synthesizing AgNPs with controlled size and geometry including chemical, green, irradiation and thermal synthesis, as well as the different types of application of AgNP...

International Journal of Molecular Sciences, 2018

Background: Sarcosine is an amino acid that is formed by methylation of glycine and is present in... more Background: Sarcosine is an amino acid that is formed by methylation of glycine and is present in trace amounts in the body. Increased sarcosine concentrations in blood plasma and urine are manifested in sarcosinemia and in some other diseases such as prostate cancer. For this purpose, sarcosine detection using the nanomedicine approach was proposed. In this study, we have prepared superparamagnetic iron oxide nanoparticles (SPIONs) with different modified surface area. Nanoparticles (NPs) were modified by chitosan (CS), and sarcosine oxidase (SOX). SPIONs without any modification were taken as controls. Methods and Results: The obtained NPs were characterized by physicochemical methods. The size of the NPs determined by the dynamic light scattering method was as follows: SPIONs/Au/NPs (100–300 nm), SPIONs/Au/CS/NPs (300–700 nm), and SPIONs/Au/CS/SOX/NPs (600–1500 nm). The amount of CS deposited on the NP surface was found to be 48 mg/mL for SPIONs/Au/CS/NPs and 39 mg/mL for SPIONs/...

Nanomaterials (Basel, Switzerland), Jan 8, 2017

This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin... more This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin for targeted cancer delivery. Prepared nanocarriers were stabilized and functionalized via zinc ions incorporated into the chitosan nanoparticle backbone. We took the advantage of high expression of sarcosine in the prostate cancer cells. The prostate cancer targeting was mediated by the AntiSar antibodies decorated surface of the nanocage. Formation of the chitosan nanoparticles was determined using a ninhydrin assay and differential pulse voltammetry. Obtained results showed the strong effect of tripolyphosphine on the nanoparticle formation. The zinc ions affected strong chitosan backbone coiling both in inner and outer chitosan nanoparticle structure. Zinc electrochemical signal depended on the level of the complex formation and the potential shift from -960 to -950 mV. Formed complex is suitable for doxorubicin delivery. It was observed the 20% entrapment efficiency of doxorubicin ...

Kvasny Prumysl, 2015

The massive industrial production and application of engineered nanomaterials ENMs currently and ... more The massive industrial production and application of engineered nanomaterials ENMs currently and the predicted increase in the near future may result in their appearance in various environments, yielding the possibility of human exposure to these ENMs through inhalation, dermal contact, or ingestion (Martirosyan, Schneider, 2014). The amount of released of ENMs into the environment cannot be accurately estimated due to the speed of production, lack of knowledge on the amount of ENMs applied and released from various applications and products. Due to high-volume production of consumer products containing ENMs, such as nanoparticles (NPs) of silver, titanium dioxide, zinc oxide, silica, etc ., human exposure to these artificial NPs is possible directly (via personal healthcare products, cosmetics, food, water, drinking, drugs and drug delivery system) and/or indirectly, e .g ., through the release of these compounds into the environment (

Nanomaterials and regenerative medicine, 2016

International Journal of Molecular Sciences, 2016

The majority of carcinomas that were developed due to the infection with human papillomavirus (HP... more The majority of carcinomas that were developed due to the infection with human papillomavirus (HPV) are caused by high-risk HPV types, HPV16 and HPV18. These HPV types contain the E6 and E7 oncogenes, so the fast detection of these oncogenes is an important point to avoid the development of cancer. Many different HPV tests are available to detect the presence of HPV in biological samples. The aim of this study was to design a fast and low cost method for HPV identification employing magnetic isolation, polymerase chain reaction (PCR) and electrochemical detection. These assays were developed to detect the interactions between E6-HPV16 oncogene and magnetizable particles (MPs) using commercial Dynabeads M-280 Streptavidin particles and laboratory-synthesized "homemade" particles called MANs (MAN-37, MAN-127 and MAN-164). The yields of PCR amplification of E6-HPV16 oncogene bound on the particles and after the elution from the particles were compared. A highest yield of E6-HPV16 DNA isolation was obtained with both MPs particles commercial M-280 Streptavidin and MAN-37 due to reducing of the interferents compared with the standard PCR method. A biosensor employing the isolation of E6-HPV16 oncogene with MPs particles followed by its electrochemical detection can be a very effective technique for HPV identification, providing simple, sensitive and cost-effective analysis.

Journal of Metallomics and Nanotechnologies, Jun 18, 2014

NANOCON 2019 Conference Proeedings

The AgNPs have considerable industrial potential and are intensively studied with regard to their... more The AgNPs have considerable industrial potential and are intensively studied with regard to their antibacterial properties. Using green synthesis, the nanoparticle surface can be coated with molecules that exhibit biologically significant properties. Increased use of nanoparticles enhances the risk of their release into the environment. However, there is still little known about the behaviour of AgNPs in the eco-environment. In this study, the effect of AgNPs prepared by green synthesis on germinated plants of maize was investigated. The effects on germination, basic growth and physiological parameters of the plant were monitored. AgNPs and silver nitrate were applied to maize seedlings (5 days in hydroponics system) at concentrations of 1 mg/L, 50 mg/L, and 150 mg/L. Chlorophyll a and b content was determined. We found that the growth inhibition of the above-ground parts of plants was about 40 % and AgNPs and silver nitrate had a significant effect on photosynthetic pigments. Chlorophyll a and b content changed with time and concentration.

International Journal of Electrochemical Science, Apr 1, 2013

Electrochemical methods are a reliable tool for a fast and low cost assay of phenolic compounds (... more Electrochemical methods are a reliable tool for a fast and low cost assay of phenolic compounds (phenolics) in food samples. The methods are precise and sesnitive enough to assay low content of polyphenols. The devices can be stationary or flow through, and based on voltammetry or amperometry. The application of voltammetric methods and HPLC-ED methods for analysis of phenolics in food samples is described in this review. The advantages and disadvantages of the methods are discussed.

Nanomaterials, Oct 31, 2019

AgNPs have attracted considerable attention in many applications including industrial use, and th... more AgNPs have attracted considerable attention in many applications including industrial use, and their antibacterial properties have been widely investigated. Due to the green synthesis process employed, the nanoparticle surface can be coated with molecules with biologically important characteristics. It has been reported that increased use of nanoparticles elevates the risk of their release into the environment. However, little is known about the behaviour of AgNPs in the eco-environment. In this study, the effect of green synthesized AgNPs on germinated plants of maize was examined. The effects on germination, basic growth and physiological parameters of the plants were monitored. Moreover, the effect of AgNPs was compared with that of Ag(I) ions in the form of AgNO 3 solution. It was found that the growth inhibition of the above-ground parts of plants was about 40%, and AgNPs exhibited a significant effect on photosynthetic pigments. Significant differences in the following parameters were observed: weights of the caryopses and fresh weight (FW) of primary roots after 96 h of exposure to Ag(I) ions and AgNPs compared to the control and between Ag compounds. In addition, the coefficient of velocity of germination (CVG) between the control and the AgNPs varied and that between the Ag(I) ions and AgNPs was also different. Phytotoxicity was proved in the following sequence: control < AgNPs < Ag(I) ions.

NANOCON 2019 Conference Proeedings, 2020

In this work, we focused on the green synthesis of silver nanoparticles (AgNPs). In the green syn... more In this work, we focused on the green synthesis of silver nanoparticles (AgNPs). In the green synthesis, plant extracts are used as reducing agents. AgNPs contain surface-bound biomolecules from the used plant extract. We used an aqueous extract from sage (Salvia officinalis). This plant is known for its high content of secondary metabolites with excellent antibacterial activity. Antibacterial activity of AgNPs prepared by green synthesis was investigated. Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli were selected for testing. Methods such as agar diffusion assay and inhibition of bacterial growth curves were used. Minimum inhibitory concentrations (MICE. coli = 250 µg/mL; MICS. aureus = 50 µg/mL) were determined. The radiuses of inhibition zones were about 1.5 cm in both bacterial strains. AgNPs prepared by this method can play an important role in dealing with the problem of bacterial resistance. Bacteria develop resistance not only to typical antibiotics. Bacterial cells are also able to eliminate the effect of AgNPs. By modifying their surface with antibacterial molecules, resistance can be effectively combatted.

NANOCON 2019 Conference Proeedings, 2020

3D printing is a modern and widely used technology that has reached virtually all sectors of huma... more 3D printing is a modern and widely used technology that has reached virtually all sectors of human activity, from the automotive industry to medical fields. Silver nanoparticles (AgNPs) have considerable industrial potential and are intensively studied regarding their antibacterial capability. Using green synthesis, the nanoparticle surface can be coated with molecules that exhibit biologically significant properties. By combining 3D printing technology and antibacterial activity of AgNPs, a unique antibacterial material can be prepared. The aim of this work was to modify the printing filament with AgNPs, to prepare the antibacterial material by 3D printing and to test the antibacterial activity of the prepared material using the JIS (Japanese industrial standards) L 1902 method. Such material can be used in hospital facilities to adjust various devices to prevent nosocomial infections.

We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/... more We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/VIS spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified fragment of DNA of 498 bp. DNA was subsequently incubated with Zn(II) ions for 60 min at 25 C. After incubation, samples were dialyzed and analysed immediately. In this way, DNA was converted into a metal bound DNA (M-DNA). Interaction of Zn(II) with DNA showed a change of the absorption spectrum (190-350 nm) and a decrease in the M-DNA melting temperature. Spectrophotometric (UV/VIS) analysis showed that increasing concentrations of Zn(II) led to an increase in absorbance at 200 nm and a decrease in absorbance at 260 nm. Application of 5.5 microM Zn(II) caused a decrease in the melting temperature (Tm) for about 7.5 C in average in comparison with control (75.5 +- 3 C). The lowest melting temperature (60.5 +- 2.5 C) was observed after application of 33 microM Zn(II). On the other hand, application of two c...

Nanomaterials, 2020

The irradiance of ultraviolet (UV) radiation is a physical parameter that significantly influence... more The irradiance of ultraviolet (UV) radiation is a physical parameter that significantly influences biological molecules by affecting their molecular structure. The influence of UV radiation on nanoparticles has not been investigated much. In this work, the ability of cadmium telluride quantum dots (CdTe QDs) to respond to natural UV radiation was examined. The average size of the yellow QDs was 4 nm, and the sizes of green, red and orange QDs were 2 nm. Quantum yield of green CdTe QDs-MSA (mercaptosuccinic acid)-A, yellow CdTe QDs-MSA-B, orange CdTe QDs-MSA-C and red CdTe QDs-MSA-D were 23.0%, 16.0%, 18.0% and 7.0%, respectively. Green, yellow, orange and red CdTe QDs were replaced every day and exposed to daily UV radiation for 12 h for seven consecutive days in summer with UV index signal integration ranging from 1894 to 2970. The rising dose of UV radiation led to the release of cadmium ions and the change in the size of individual QDs. The shifts were evident in absorption signa...

International Journal of Electrochemical Science

Electrochemical methods are a reliable tool for a fast and low cost assay of phenolic compounds (... more Electrochemical methods are a reliable tool for a fast and low cost assay of phenolic compounds (phenolics) in food samples. The methods are precise and sesnitive enough to assay low content of polyphenols. The devices can be stationary or flow through, and based on voltammetry or amperometry. The application of voltammetric methods and HPLC-ED methods for analysis of phenolics in food samples is described in this review. The advantages and disadvantages of the methods are discussed.

Lipid Peroxidation, 2012

Lipid Peroxidation 132 with another molecule of fatty acid, from which a hydrogen atom is detache... more Lipid Peroxidation 132 with another molecule of fatty acid, from which a hydrogen atom is detached under formation of lipid hydroperoxide from original molecule (3). After pairing of all radicals, the last stage of the reaction called termination occurs. In addition to the above-mentioned chemical non-enzymatic peroxidation, enzymatic lipid peroxidation that is catalysed by the enzymes cyclooxygenase and lipoxygenase takes place. [26,28]. Both enzymes are involved in the formation of eicosanoids, which represent a group of biologically active lipid compounds derived from unsaturated fatty acids containing 20 carbon atoms. Cyclooxygenase is involved in the genesis of prostaglandins [29].

Journal of nanobiotechnology, Jan 26, 2017

Nanomaterials in agriculture are becoming popular due to the impressive advantages of these parti... more Nanomaterials in agriculture are becoming popular due to the impressive advantages of these particles. However, their bioavailability and toxicity are key features for their massive employment. Herein, we comprehensively summarize the latest findings on the phytotoxicity of nanomaterial products based on essential metals used in plant protection. The metal nanoparticles (NPs) synthesized from essential metals belong to the most commonly manufactured types of nanomaterials since they have unique physical and chemical properties and are used in agricultural and biotechnological applications, which are discussed. The paper discusses the interactions of nanomaterials and vascular plants, which are the subject of intensive research because plants closely interact with soil, water, and atmosphere; they are also part of the food chain. Regarding the accumulation of NPs in the plant body, their quantification and localization is still very unclear and further research in this area is necess...

Abstract: Polymers are currently widely used to replace a variety of natural materials with respe... more Abstract: Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of application of polymers is the production of different products for medical use. In this case, it is necessary to face a significant disadvantage of polymer products due to possible and very common colonization of the surface by various microorganisms that can pose a potential danger to the patient. One of the possible solutions is to prepare polymer with antibacterial/antimicrobial properties that is resistant to bacterial colonization. The aim of this study was to contribute to the development of antimicrobial polymeric material ideal for covering vascular implants with subsequent use in transplant surgery. Therefore, the complexes of polymeric substances (hyaluronic acid and chitosan) with silver nitrate orInt. J. Mol. Sci. 2013, 14 13593

In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(hi... more In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(his)Cl 2 , Zn(his)Cl 2) and ZnS quantum dots (QDs) using printed electrodes. This method was chosen due to the simple (easy to use) instrumentation and variable setting of flows. Reduction signals of zinc under the strictly defined and controlled conditions (pH, temperature, flow rate, accumulation time and applied potential) were studied. We showed that the increasing concentration of the complexes (Zn(phen)(his)Cl 2 , Zn(his)Cl 2) led to a decrease in the electrochemical signal and a significant shift of the potential to more positive values. The most likely explanation of this result is that zinc is strongly bound in the complex and its distribution on the electrode is very limited. Changing the pH from 3.5 to 5.5 resulted in a significant intensification of the Zn(II)

1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemed... more 1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, European Union 2 Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ616 00 Brno, Czech Republic, European Union 3 Vysoka skola Karla Englise, Sujanovo square 356/1, CZ-602 00, Brno, Czech Republic, European Union * E-mail: vojtech.adam@mendelu.cz

Pharmaceutics, 2020

Silver nanoparticles (AgNPs) have recently become very attractive for the scientific community du... more Silver nanoparticles (AgNPs) have recently become very attractive for the scientific community due to their broad spectrum of applications in the biomedical field. The main advantages of AgNPs include a simple method of synthesis, a simple way to change their morphology and high surface area to volume ratio. Much research has been carried out over the years to evaluate their possible effectivity against microbial organisms. The most important factors which influence the effectivity of AgNPs against microorganisms are the method of their preparation and the type of application. When incorporated into fabric wound dressings and other textiles, AgNPs have shown significant antibacterial activity against both Gram-positive and Gram-negative bacteria and inhibited biofilm formation. In this review, the different routes of synthesizing AgNPs with controlled size and geometry including chemical, green, irradiation and thermal synthesis, as well as the different types of application of AgNP...

International Journal of Molecular Sciences, 2018

Background: Sarcosine is an amino acid that is formed by methylation of glycine and is present in... more Background: Sarcosine is an amino acid that is formed by methylation of glycine and is present in trace amounts in the body. Increased sarcosine concentrations in blood plasma and urine are manifested in sarcosinemia and in some other diseases such as prostate cancer. For this purpose, sarcosine detection using the nanomedicine approach was proposed. In this study, we have prepared superparamagnetic iron oxide nanoparticles (SPIONs) with different modified surface area. Nanoparticles (NPs) were modified by chitosan (CS), and sarcosine oxidase (SOX). SPIONs without any modification were taken as controls. Methods and Results: The obtained NPs were characterized by physicochemical methods. The size of the NPs determined by the dynamic light scattering method was as follows: SPIONs/Au/NPs (100–300 nm), SPIONs/Au/CS/NPs (300–700 nm), and SPIONs/Au/CS/SOX/NPs (600–1500 nm). The amount of CS deposited on the NP surface was found to be 48 mg/mL for SPIONs/Au/CS/NPs and 39 mg/mL for SPIONs/...

Nanomaterials (Basel, Switzerland), Jan 8, 2017

This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin... more This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin for targeted cancer delivery. Prepared nanocarriers were stabilized and functionalized via zinc ions incorporated into the chitosan nanoparticle backbone. We took the advantage of high expression of sarcosine in the prostate cancer cells. The prostate cancer targeting was mediated by the AntiSar antibodies decorated surface of the nanocage. Formation of the chitosan nanoparticles was determined using a ninhydrin assay and differential pulse voltammetry. Obtained results showed the strong effect of tripolyphosphine on the nanoparticle formation. The zinc ions affected strong chitosan backbone coiling both in inner and outer chitosan nanoparticle structure. Zinc electrochemical signal depended on the level of the complex formation and the potential shift from -960 to -950 mV. Formed complex is suitable for doxorubicin delivery. It was observed the 20% entrapment efficiency of doxorubicin ...

Kvasny Prumysl, 2015

The massive industrial production and application of engineered nanomaterials ENMs currently and ... more The massive industrial production and application of engineered nanomaterials ENMs currently and the predicted increase in the near future may result in their appearance in various environments, yielding the possibility of human exposure to these ENMs through inhalation, dermal contact, or ingestion (Martirosyan, Schneider, 2014). The amount of released of ENMs into the environment cannot be accurately estimated due to the speed of production, lack of knowledge on the amount of ENMs applied and released from various applications and products. Due to high-volume production of consumer products containing ENMs, such as nanoparticles (NPs) of silver, titanium dioxide, zinc oxide, silica, etc ., human exposure to these artificial NPs is possible directly (via personal healthcare products, cosmetics, food, water, drinking, drugs and drug delivery system) and/or indirectly, e .g ., through the release of these compounds into the environment (

Nanomaterials and regenerative medicine, 2016

International Journal of Molecular Sciences, 2016

The majority of carcinomas that were developed due to the infection with human papillomavirus (HP... more The majority of carcinomas that were developed due to the infection with human papillomavirus (HPV) are caused by high-risk HPV types, HPV16 and HPV18. These HPV types contain the E6 and E7 oncogenes, so the fast detection of these oncogenes is an important point to avoid the development of cancer. Many different HPV tests are available to detect the presence of HPV in biological samples. The aim of this study was to design a fast and low cost method for HPV identification employing magnetic isolation, polymerase chain reaction (PCR) and electrochemical detection. These assays were developed to detect the interactions between E6-HPV16 oncogene and magnetizable particles (MPs) using commercial Dynabeads M-280 Streptavidin particles and laboratory-synthesized "homemade" particles called MANs (MAN-37, MAN-127 and MAN-164). The yields of PCR amplification of E6-HPV16 oncogene bound on the particles and after the elution from the particles were compared. A highest yield of E6-HPV16 DNA isolation was obtained with both MPs particles commercial M-280 Streptavidin and MAN-37 due to reducing of the interferents compared with the standard PCR method. A biosensor employing the isolation of E6-HPV16 oncogene with MPs particles followed by its electrochemical detection can be a very effective technique for HPV identification, providing simple, sensitive and cost-effective analysis.

Journal of Metallomics and Nanotechnologies, Jun 18, 2014