Brenda Gomes - Academia.edu (original) (raw)

Papers by Brenda Gomes

Research paper thumbnail of Correlation between Clinical/Radiographic Features and Inflammatory Cytokine Networks Produced by Macrophages Stimulated with Endodontic Content

Journal of Endodontics, 2012

Research paper thumbnail of Antigenic Activity of Bacterial Endodontic Contents from Primary Root Canal Infection with Periapical Lesions against Macrophage in the Release of Interleukin-1β and Tumor Necrosis Factor α

Journal of Endodontics, 2010

Research paper thumbnail of Antigenicity of Primary Endodontic Infection against Macrophages by the Levels of PGE2 Production

Journal of Endodontics, 2011

Research paper thumbnail of Capsule Locus Polymorphism among Distinct Lineages of Enterococcus faecalis Isolated from Canals of Root-filled Teeth with Periapical Lesions

Journal of Endodontics, 2012

Research paper thumbnail of Quantification of Endotoxins in Infected Root Canals and Acute Apical Abscess Exudates: Monitoring the Effectiveness of Root Canal Procedures in the Reduction of Endotoxins

Journal of Endodontics, 2014

Research paper thumbnail of Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

Journal of Endodontics, 2008

Research paper thumbnail of Persistent Extraradicular Infection in Root-filled Asymptomatic Human Tooth: Scanning Electron Microscopic Analysis and Microbial Investigation after Apical Microsurgery

Journal of Endodontics, 2011

Research paper thumbnail of Quantification of Endotoxins and Cultivable Bacteria in Root Canal Infection before and after Chemomechanical Preparation with 2.5% Sodium Hypochlorite

Journal of Endodontics, 2008

Research paper thumbnail of Comparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections

Journal of Endodontics, 2012

Research paper thumbnail of Effect of Different Irrigation Protocols on Resin Sealer Bond Strength to Dentin

Journal of Endodontics, 2013

Research paper thumbnail of Comparison of 2.5% Sodium Hypochlorite and 2% Chlorhexidine Gel on Oral Bacterial Lipopolysaccharide Reduction from Primarily Infected Root Canals

Journal of Endodontics, 2009

Research paper thumbnail of Molecular Fingerprinting Reveals the Presence of Unique Communities Associated with Paired Samples of Root Canals and Acute Apical Abscesses

Journal of Endodontics, 2010

Research paper thumbnail of Endodontic Microflora of Different Teeth in the Same Mouth

Research paper thumbnail of Endodontic Microflora of Different Teeth in the Same Mouth

Anaerobe, 1999

This study examined similarities in the microbiology of initial, and subsequent, infections in di... more This study examined similarities in the microbiology of initial, and subsequent, infections in different dental root canals in the same patient. In the initial infections, 61.5% of root canals had anaerobes present compared with 54% of root canals treated subsequently. Commercial identification kits were employed in speciation of isolates. Both sets of dental root canals had in common Peptostreptococcus micros, Eubacterium lentum and Veillonella parvula as species very commonly isolated. In addition Prevotella spp. tended to be present in both initial and subsequently infected teeth in the same patient (P=0.01); this was true of Gram-negative bacteria in general (P=0.03). A significant association was observed between the type of tooth infected at an initial and subsequent infection in the same mouth (P=0.01). In conclusion, there is an association between bacteria isolated from initial endodontically infected teeth and subsequent infected root canals in a patient.

Research paper thumbnail of Quantification of Endotoxins and Cultivable Bacteria in Root Canal Infection before and after Chemomechanical Preparation with 2.5% Sodium Hypochlorite

Journal of Endodontics, 2008

Research paper thumbnail of Persistent Extraradicular Infection in Root-Filled Asymptomatic Human Tooth: Scanning Electron Microscopic Analysis and Microbial Investigation After Apical Microsurgery

Journal of Endodontics, 2000

Procedural accidents have a negative effect on healing and might contribute to the persistence of... more Procedural accidents have a negative effect on healing and might contribute to the persistence of infections in inaccessible apical areas, requiring surgical intervention. This report describes a case of persistent apical periodontitis of a lower left first molar associated with the sinus tract and a periapical lesion that required nonsurgical endodontic retreatment and apical surgery for resolution.The tooth had received endodontic treatment 3 years ago and had to be retreated using the crown-down technique with chemical auxiliary substance (2% chlorhexidine gel), foramen patency, and enlargement and was filled in a single appointment. The occlusal access cavity was immediately restored with composite resin. After 1 month, it could be observed that the sinus tract persisted and, radiographically, the lesion remained unaltered. Therefore, endodontic microsurgery was indicated. Apical microsurgery was performed under magnification with the use of a dental operating microscope including apicectomy, root end with ultrasound, and sealing with mineral trioxide aggregate. A microbiological sample was collected from the apical lesion. The resected distal root apex was observed by scanning electron microscopy.The following species were detected: Actinomyces naeslundii and Actinomyces meyeri, Propionibacterium propionicum, Clostridium botullinum, Parvimonas micra, and Bacteroides ureolyticus; scanning electron microscopic analysis revealed bacterial biofilm surrounding the apical foramen and external radicular surface. Gutta-percha overfilling at the apex because of a zip caused during initial endodontic treatment could be observed. A 6-month follow-up showed apparent radiographic periapical healing, which progressed after 24 months.Gram-positive anaerobic bacteria and extraradicular biofilm seem to participate in the maintenance of persistent periapical pathology, and endodontic retreatment followed by periapical microsurgery proved to be a successful alternative in the resolution of persistent extraradicular infections.

Research paper thumbnail of Interactions between Irrigants Commonly Used in Endodontic Practice: A Chemical Analysis

Journal of Endodontics, 2013

The aim of this work was to characterize the by-products formed in the associations between the m... more The aim of this work was to characterize the by-products formed in the associations between the most commonly used irrigants in endodontic practice through electrospray ionization quadrupole time-of-flight mass spectrometry analyses. Sodium hypochlorite (NaOCl) (0.16%, 1%, 2.5%, and 5.25%) was associated with 2% chlorhexidine (CHX) solution and gel, 17% EDTA, 10% citric acid, 37% phosphoric acid, saline solution, ethanol, and distilled water. CHX solution and gel were also associated with all above mentioned irrigants. The solutions were mixed in a 1:1 ratio, and electrospray ionization quadrupole time-of-flight mass spectrometry was used to characterize the precipitates when formed. CHX produced an orange-brown precipitate when associated with NaOCl from 1%-5.25% and an orange-white precipitate when associated with 0.16% NaOCl. When associated with EDTA, CHX produced a white milky precipitate, and when associated with saline solution and ethanol, a salt precipitation was produced. No precipitation was observed when CHX was associated with citric acid, phosphoric acid, or distilled water. In the NaOCl associations, precipitation occurred only when CHX was present. The orange-brown precipitate observed in the association between CHX and NaOCl occurs because of the presence of NaOCl, an oxidizing agent causing chlorination of the guanidino nitrogens of the CHX. The precipitates formed in the reaction of CHX with EDTA, saline solution, and ethanol were associated with acid-base reactions, salting-out process, and lower solubility, respectively. NaOCl associated with EDTA, citric acid, and phosphoric acid leads mainly to chlorine gas formation. Intermediate flushes with distilled water seem to be appropriate to prevent or at least reduce formation of by-products.

Research paper thumbnail of Scanning Electron Microscopic Investigation of the Effectiveness of Phosphoric Acid in Smear Layer Removal When Compared with EDTA and Citric Acid

Journal of Endodontics, 2011

Research paper thumbnail of A Comparative Study of the Smear Layer Removal Using Scanning Electronic Microscopy and Atomic Force Microscopy

Journal of Advanced Microscopy Research, 2012

Research paper thumbnail of Repair of apical root resorption associated with periodontitis using a new intracanal medicament protocol

Journal of oral science, 2014

Endodontic therapy is indicated for cases of traumatic tooth dislocation associated with pulp nec... more Endodontic therapy is indicated for cases of traumatic tooth dislocation associated with pulp necrosis and/or inflammatory resorption. Here we describe the management of a maxillary left lateral permanent incisor that suffered lateral luxation, leading to pulp necrosis and root resorption, in a 13-year-old boy. The traumatized tooth was treated successfully by intracanal medication with calcium hydroxide, 2% chlorhexidine gel and zinc oxide for 12 months without any need to change the dressing, followed by conventional root canal filling. The postoperative course was uneventful and a stable clinical outcome was obtained with evidence of periapical lesion repair and stabilization of the resorption process.

Research paper thumbnail of Correlation between Clinical/Radiographic Features and Inflammatory Cytokine Networks Produced by Macrophages Stimulated with Endodontic Content

Journal of Endodontics, 2012

Research paper thumbnail of Antigenic Activity of Bacterial Endodontic Contents from Primary Root Canal Infection with Periapical Lesions against Macrophage in the Release of Interleukin-1β and Tumor Necrosis Factor α

Journal of Endodontics, 2010

Research paper thumbnail of Antigenicity of Primary Endodontic Infection against Macrophages by the Levels of PGE2 Production

Journal of Endodontics, 2011

Research paper thumbnail of Capsule Locus Polymorphism among Distinct Lineages of Enterococcus faecalis Isolated from Canals of Root-filled Teeth with Periapical Lesions

Journal of Endodontics, 2012

Research paper thumbnail of Quantification of Endotoxins in Infected Root Canals and Acute Apical Abscess Exudates: Monitoring the Effectiveness of Root Canal Procedures in the Reduction of Endotoxins

Journal of Endodontics, 2014

Research paper thumbnail of Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

Journal of Endodontics, 2008

Research paper thumbnail of Persistent Extraradicular Infection in Root-filled Asymptomatic Human Tooth: Scanning Electron Microscopic Analysis and Microbial Investigation after Apical Microsurgery

Journal of Endodontics, 2011

Research paper thumbnail of Quantification of Endotoxins and Cultivable Bacteria in Root Canal Infection before and after Chemomechanical Preparation with 2.5% Sodium Hypochlorite

Journal of Endodontics, 2008

Research paper thumbnail of Comparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections

Journal of Endodontics, 2012

Research paper thumbnail of Effect of Different Irrigation Protocols on Resin Sealer Bond Strength to Dentin

Journal of Endodontics, 2013

Research paper thumbnail of Comparison of 2.5% Sodium Hypochlorite and 2% Chlorhexidine Gel on Oral Bacterial Lipopolysaccharide Reduction from Primarily Infected Root Canals

Journal of Endodontics, 2009

Research paper thumbnail of Molecular Fingerprinting Reveals the Presence of Unique Communities Associated with Paired Samples of Root Canals and Acute Apical Abscesses

Journal of Endodontics, 2010

Research paper thumbnail of Endodontic Microflora of Different Teeth in the Same Mouth

Research paper thumbnail of Endodontic Microflora of Different Teeth in the Same Mouth

Anaerobe, 1999

This study examined similarities in the microbiology of initial, and subsequent, infections in di... more This study examined similarities in the microbiology of initial, and subsequent, infections in different dental root canals in the same patient. In the initial infections, 61.5% of root canals had anaerobes present compared with 54% of root canals treated subsequently. Commercial identification kits were employed in speciation of isolates. Both sets of dental root canals had in common Peptostreptococcus micros, Eubacterium lentum and Veillonella parvula as species very commonly isolated. In addition Prevotella spp. tended to be present in both initial and subsequently infected teeth in the same patient (P=0.01); this was true of Gram-negative bacteria in general (P=0.03). A significant association was observed between the type of tooth infected at an initial and subsequent infection in the same mouth (P=0.01). In conclusion, there is an association between bacteria isolated from initial endodontically infected teeth and subsequent infected root canals in a patient.

Research paper thumbnail of Quantification of Endotoxins and Cultivable Bacteria in Root Canal Infection before and after Chemomechanical Preparation with 2.5% Sodium Hypochlorite

Journal of Endodontics, 2008

Research paper thumbnail of Persistent Extraradicular Infection in Root-Filled Asymptomatic Human Tooth: Scanning Electron Microscopic Analysis and Microbial Investigation After Apical Microsurgery

Journal of Endodontics, 2000

Procedural accidents have a negative effect on healing and might contribute to the persistence of... more Procedural accidents have a negative effect on healing and might contribute to the persistence of infections in inaccessible apical areas, requiring surgical intervention. This report describes a case of persistent apical periodontitis of a lower left first molar associated with the sinus tract and a periapical lesion that required nonsurgical endodontic retreatment and apical surgery for resolution.The tooth had received endodontic treatment 3 years ago and had to be retreated using the crown-down technique with chemical auxiliary substance (2% chlorhexidine gel), foramen patency, and enlargement and was filled in a single appointment. The occlusal access cavity was immediately restored with composite resin. After 1 month, it could be observed that the sinus tract persisted and, radiographically, the lesion remained unaltered. Therefore, endodontic microsurgery was indicated. Apical microsurgery was performed under magnification with the use of a dental operating microscope including apicectomy, root end with ultrasound, and sealing with mineral trioxide aggregate. A microbiological sample was collected from the apical lesion. The resected distal root apex was observed by scanning electron microscopy.The following species were detected: Actinomyces naeslundii and Actinomyces meyeri, Propionibacterium propionicum, Clostridium botullinum, Parvimonas micra, and Bacteroides ureolyticus; scanning electron microscopic analysis revealed bacterial biofilm surrounding the apical foramen and external radicular surface. Gutta-percha overfilling at the apex because of a zip caused during initial endodontic treatment could be observed. A 6-month follow-up showed apparent radiographic periapical healing, which progressed after 24 months.Gram-positive anaerobic bacteria and extraradicular biofilm seem to participate in the maintenance of persistent periapical pathology, and endodontic retreatment followed by periapical microsurgery proved to be a successful alternative in the resolution of persistent extraradicular infections.

Research paper thumbnail of Interactions between Irrigants Commonly Used in Endodontic Practice: A Chemical Analysis

Journal of Endodontics, 2013

The aim of this work was to characterize the by-products formed in the associations between the m... more The aim of this work was to characterize the by-products formed in the associations between the most commonly used irrigants in endodontic practice through electrospray ionization quadrupole time-of-flight mass spectrometry analyses. Sodium hypochlorite (NaOCl) (0.16%, 1%, 2.5%, and 5.25%) was associated with 2% chlorhexidine (CHX) solution and gel, 17% EDTA, 10% citric acid, 37% phosphoric acid, saline solution, ethanol, and distilled water. CHX solution and gel were also associated with all above mentioned irrigants. The solutions were mixed in a 1:1 ratio, and electrospray ionization quadrupole time-of-flight mass spectrometry was used to characterize the precipitates when formed. CHX produced an orange-brown precipitate when associated with NaOCl from 1%-5.25% and an orange-white precipitate when associated with 0.16% NaOCl. When associated with EDTA, CHX produced a white milky precipitate, and when associated with saline solution and ethanol, a salt precipitation was produced. No precipitation was observed when CHX was associated with citric acid, phosphoric acid, or distilled water. In the NaOCl associations, precipitation occurred only when CHX was present. The orange-brown precipitate observed in the association between CHX and NaOCl occurs because of the presence of NaOCl, an oxidizing agent causing chlorination of the guanidino nitrogens of the CHX. The precipitates formed in the reaction of CHX with EDTA, saline solution, and ethanol were associated with acid-base reactions, salting-out process, and lower solubility, respectively. NaOCl associated with EDTA, citric acid, and phosphoric acid leads mainly to chlorine gas formation. Intermediate flushes with distilled water seem to be appropriate to prevent or at least reduce formation of by-products.

Research paper thumbnail of Scanning Electron Microscopic Investigation of the Effectiveness of Phosphoric Acid in Smear Layer Removal When Compared with EDTA and Citric Acid

Journal of Endodontics, 2011

Research paper thumbnail of A Comparative Study of the Smear Layer Removal Using Scanning Electronic Microscopy and Atomic Force Microscopy

Journal of Advanced Microscopy Research, 2012

Research paper thumbnail of Repair of apical root resorption associated with periodontitis using a new intracanal medicament protocol

Journal of oral science, 2014

Endodontic therapy is indicated for cases of traumatic tooth dislocation associated with pulp nec... more Endodontic therapy is indicated for cases of traumatic tooth dislocation associated with pulp necrosis and/or inflammatory resorption. Here we describe the management of a maxillary left lateral permanent incisor that suffered lateral luxation, leading to pulp necrosis and root resorption, in a 13-year-old boy. The traumatized tooth was treated successfully by intracanal medication with calcium hydroxide, 2% chlorhexidine gel and zinc oxide for 12 months without any need to change the dressing, followed by conventional root canal filling. The postoperative course was uneventful and a stable clinical outcome was obtained with evidence of periapical lesion repair and stabilization of the resorption process.