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Papers by Brian Burke
Cell, 2002
filament proteins and plays an essential role in the main-University of Calgary tenance of NE int... more filament proteins and plays an essential role in the main-University of Calgary tenance of NE integrity and nuclear organization (Gruen-3330 Hospital Drive NW baum et al., 2000; Wilson et al., 2001). Calgary, Alberta T2N 4N1 Prophase in higher cells is defined by condensation Canada of chromatin, and initiation of events leading to NE 2 Department of Biology breakdown (NEB). NEB involves the disassembly and 220A Mudd Hall dispersal of all major NE structural components (Lee The Johns Hopkins University et alSummary Snow et al., 1987), and membranes. The disruption of the nuclear membranes marks the end of prophase. At During prophase in higher cells, centrosomes localize this time, integral proteins of the INM and NPCs are to deep invaginations in the nuclear envelope in a microlost from the nuclear periphery and become distributed tubule-dependent process. Loss of nuclear membranes throughout the cell (Chaudhary and Courvalin, 1993; Elin prometaphase commences in regions of the nuclear lenberg et al., 1997; Yang et al., 1997)
Science, 2008
Fluorescence light microscopy allows multicolor visualization of cellular components with high sp... more Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.
Cell, 2002
filament proteins and plays an essential role in the main-University of Calgary tenance of NE int... more filament proteins and plays an essential role in the main-University of Calgary tenance of NE integrity and nuclear organization (Gruen-3330 Hospital Drive NW baum et al., 2000; Wilson et al., 2001). Calgary, Alberta T2N 4N1 Prophase in higher cells is defined by condensation Canada of chromatin, and initiation of events leading to NE 2 Department of Biology breakdown (NEB). NEB involves the disassembly and 220A Mudd Hall dispersal of all major NE structural components (Lee The Johns Hopkins University et alSummary Snow et al., 1987), and membranes. The disruption of the nuclear membranes marks the end of prophase. At During prophase in higher cells, centrosomes localize this time, integral proteins of the INM and NPCs are to deep invaginations in the nuclear envelope in a microlost from the nuclear periphery and become distributed tubule-dependent process. Loss of nuclear membranes throughout the cell (Chaudhary and Courvalin, 1993; Elin prometaphase commences in regions of the nuclear lenberg et al., 1997; Yang et al., 1997)
Science, 2008
Fluorescence light microscopy allows multicolor visualization of cellular components with high sp... more Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.