Brian Derby - Academia.edu (original) (raw)
Papers by Brian Derby
Investigative Ophthalmology & Visual Science, 2013
Current Eye Research, 2013
Lab on a chip, Jan 20, 2015
We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human ne... more We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human neuroprogenitor cells (NPCs) derived from human embryonic stem cells (hESCs). Sessile drops of volume 114 nl were formed by printing cell suspensions containing dimethyl sulphoxide (DMSO) as a cryoprotection agent (CPA) at rates in the region 100 Hz-20 kHz, from individual droplets of 380 pl. After printing and a freeze/thaw cycle (with a minimum 24 hours hold period at liquid N2 temperature), 3T3 cells showed an average viability of >90% with CPA concentration <0.8 M at all drop deposition rates. This is a significantly lower CPA concentration than normally used with conventional cryopreservation methods. Cell viability shows a small variation with the polymer substrates used, with the best results obtained using a polyimide substrate. The viability of 3T3 cells after 2 months storage at liquid nitrogen temperature was slightly reduced compared to the cells held for 24 hours but the...
International Journal of Materials and Product Technology, 2001
Ceramic Microstructures ’86, 1987
Solomah/Indentation, 2006
MRS Proceedings, 2014
ABSTRACT
Journal of materials science. Materials in medicine, 2002
Tricalcium phosphate (TCP) scaffolds with controlled internal porosity were fabricated with a sus... more Tricalcium phosphate (TCP) scaffolds with controlled internal porosity were fabricated with a suspension of TCP in diacrylate cross-linking monomers using a mold prepared by ink-jet printing. Scaffolds were removed by selective dissolution of the mold. They were heat treated for removal of the acrylic binder followed by sintering. Despite a considerable linear shrinkage, scaffold porosity was retained after sintering. Composite scaffolds were fabricated from TCP in poly(ethylene glycol) diacrylate using an identical gel casting route.
Scripta Metallurgica, 1988
Investigative Ophthalmology & Visual Science, 2013
Current Eye Research, 2013
Lab on a chip, Jan 20, 2015
We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human ne... more We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human neuroprogenitor cells (NPCs) derived from human embryonic stem cells (hESCs). Sessile drops of volume 114 nl were formed by printing cell suspensions containing dimethyl sulphoxide (DMSO) as a cryoprotection agent (CPA) at rates in the region 100 Hz-20 kHz, from individual droplets of 380 pl. After printing and a freeze/thaw cycle (with a minimum 24 hours hold period at liquid N2 temperature), 3T3 cells showed an average viability of >90% with CPA concentration <0.8 M at all drop deposition rates. This is a significantly lower CPA concentration than normally used with conventional cryopreservation methods. Cell viability shows a small variation with the polymer substrates used, with the best results obtained using a polyimide substrate. The viability of 3T3 cells after 2 months storage at liquid nitrogen temperature was slightly reduced compared to the cells held for 24 hours but the...
International Journal of Materials and Product Technology, 2001
Ceramic Microstructures ’86, 1987
Solomah/Indentation, 2006
MRS Proceedings, 2014
ABSTRACT
Journal of materials science. Materials in medicine, 2002
Tricalcium phosphate (TCP) scaffolds with controlled internal porosity were fabricated with a sus... more Tricalcium phosphate (TCP) scaffolds with controlled internal porosity were fabricated with a suspension of TCP in diacrylate cross-linking monomers using a mold prepared by ink-jet printing. Scaffolds were removed by selective dissolution of the mold. They were heat treated for removal of the acrylic binder followed by sintering. Despite a considerable linear shrinkage, scaffold porosity was retained after sintering. Composite scaffolds were fabricated from TCP in poly(ethylene glycol) diacrylate using an identical gel casting route.
Scripta Metallurgica, 1988
Materials World Volume 8, Issue 7, 2000, Pages 20-22, Jan 1, 2000
Studying materials microstructures is extremely important. They form a vital link in the processi... more Studying materials microstructures is extremely important. They form a vital link in the processing/properties/structure inter-relationship that is at the heart of materials science. To further improve probing into the micro world, UMIST and the University of Manchester are using the Internet to provide a hands-on educational resource otherwise unavailable to students. The venture, the Internet Microscope, is a teaching resource to help students learn about microstructures, an image library that attempts to reproduce most of the features of a real microscope.