Bruno Henriques - Academia.edu (original) (raw)

Papers by Bruno Henriques

Ceramics, 2022

The aim of this study was to conduct an integrative review of the biological and mechanical outco... more The aim of this study was to conduct an integrative review of the biological and mechanical outcomes of porous zirconia structures for extensive bone repair. An electronic search was performed on the PubMed database using a combination of the following scientific terms: porous, scaffold, foam, zirconia, bone regeneration, bone repair, bone healing. Articles published in the English language up to December 2021 and related to porosity, pore interconnectivity, biocompatibility and strength of the material, and the manufacturing methods of zirconia porous structures were included. Randomized controlled trials and prospective cohort studies were also evaluated. The research identified 145 studies, of which 23 were considered relevant. A high percentage of pores and the size and interconnectivity of pores are key factors for cell migration, attachment, proliferation, and differentiation. In addition, pore interconnectivity allows for the exchange of nutrients between cells and formation ...

Dental porcelain composites reinforced and toughened by 20 wt.% tetragonal zirconia (3Y... more Dental porcelain composites reinforced and toughened by 20 wt.% tetragonal zirconia (3Y-TZP) were processed by hot pressing at 1000°C. Two types of particles were tested: yttriastabilized zirconia (ZrO2–3%Y2O3) agglomerates and pre-sintered yttria-stabilized zirconia (ZrO2–3%Y2O3) particles. The composites as well as the reinforcing particles were analyzed by the means of optical and Scanning Electron Microscopy (SEM), Energy Dispersion Spectroscopy (EDS) and X-Ray Diffraction (XRD). The mechanical properties were obtained by the transverse rupture strength test. Wear tests were also performed on the composites and monolithic porcelain. The best mechanical results were displayed by the porcelain reinforced with the pre-sintered ZrO2–3%Y2O3 agglomerates.

Clinical Oral Investigations, 2021

Objective: The objective of this study was to perform a systematic review on the toxic effect of ... more Objective: The objective of this study was to perform a systematic review on the toxic effect of submicron and nano-scale commercially pure titanium (cp Ti) debris on cells of peri-implant tissues. Materials and Methods: A systematic review was carried out on the PUBMED electronic platform using the following keywords: Ti "OR" titanium "AND" dental implants "AND" nanoparticles "OR" nano-scale debris "OR" nanometric debris "AND" osteoblasts "OR "Cytotoxicity" OR "mutagenic" Results: Titanium nanoparticles in submicron-and nano-scale altered the behavior of cells in culture medium. An inflammatory response was triggered by macrophages, fibroblasts, osteoblasts, mesenchymal cells, and odontoblasts as indicated by the detection of several inflammatory mediators: IL-6, IL-1β, TNF-α and PGE2. The formation of a rich bioactive complex composed of calcium and phosphorus on titanium nanoparticles allowed the binding to proteins leading the cell internalization phenomenon. The nano-particles induced mutagenic and carcinogenic effects into the cells. Conclusions: The cytotoxic effect of debris released from dental implants depends on the size, concentrations, and chemical composition of the particles. A high concentration of particles on nanometric scale intensifies the inflammatory responses with mutagenic potential of the surrounding cells. Clinical relevance: Titanium (Ti) ions and debris have been detected in peri-implant tissues with different sizes and forms. The presence of metallic debris at peri-implant tissues also stimulates the migration of immune cells and inflamatory reactions. Cp Ti and TiO 2 micro-and nano-scale particles can reach the blood stream, accumulating in lungs, liver, spleen, and bone marrow.

The present chapter introduces the importance and concepts of nanostructured biomaterials and sur... more The present chapter introduces the importance and concepts of nanostructured biomaterials and surfaces for oral and cranio-maxillofacial applications. A brief description is devoted on implant surfaces, bioactive ceramics, composites, and functionally graded materials to bring readers with different backgrounds to a common level of knowledge on nanostructured biomaterials.

International Journal of Business Information Systems, 2021

Chemosphere, 2021

The high demand for greener energy and technological innovation require some crucial elements, su... more The high demand for greener energy and technological innovation require some crucial elements, such as the rare earths Nd and Dy. Being considered two of the most critical elements (high supply risk), it is vital to recover them from wastes/wastewaters, for later reuse. Here, the influence of operational parameters, such as biosorbent stock density (0.5, 3.0, and 5.5 g L-1), ionic strength (salinity 10 and 30) and contact time (24, 72 and 168 h), in the biosorption/bioaccumulation of Nd and Dy by two living marine macroalgae was evaluated in artificial seawater, seeking the improvement of the process. Results demonstrated that stock density is the most influential parameter, while the ionic strength showed to be a selective parameter, with a major influence only for Dy removal, which can be attributed to the different chemical characteristics observed between light rare earth elements (LREE) and heavy rare earth elements (HREE). For the ranges studied, the greatest removal/recovery for Gracilaria sp. was achieved with a stock density of 3.0 g L-1 at salinity 10, after 72 h for both REEs. For Ulva lactuca optimal conditions were: stock density of 5.5 g L-1 at salinity 10 with a contact time of 72 h for both REEs. Between species, U. lactuca showed to be the most promising, with removal efficiencies up to 98% for Nd and 89% for Dy. Findings substantiate the potential of the proposed process for obtaining Nd and Dy from secondary sources, particularly from low-level contaminated waters.

Journal of Dentistry, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Wear, 2020

The main aim of the present study was to evaluate the micro-scale abrasion and sliding wear behav... more The main aim of the present study was to evaluate the micro-scale abrasion and sliding wear behavior of a zirconium-lithium silicate (ZLS) glass-ceramic and a polymer-infiltrated ceramic network used in dentistry. Samples were assessed on a reciprocating ball-on-plate tribometer at 30 N normal load, 1 Hz and stroke length of 2 mm. The wear sliding tests were carried out against an alumina ball in artificial saliva at 37 � C. Also, microscale abrasion tests were performed in the presence of abrasive particles to simulate three-body abrasion conditions. The micro-scale abrasion tests were performed at 60 rpm on 0.8 N normal load for 600 revolutions of a stainless-steel ball in contact with a suspension of hydrated silica particles. After wear tests, the worn surfaces were inspected by scanning electron microscopy (SEM). Abrasion was the main wear mechanism found during the tests. However, the hybrid ceramic revealed an unstable adhesive tribo-layer, associated with a delamination process. The wear volume mean values recorded after the micro-abrasion tests were significantly higher for the polymer infiltrated ceramic network (~1.44 x10-1 mm 3) than those for the ZLS glass-ceramic (~9.89 x10-2 mm 3). Also, the mean values of specific sliding wear rate were higher for the polymer-infiltrated ceramic network (~5.33 x10-5 mm 3 /N.m) than those for the ZLS glass-ceramic ZL(~3.17 x10-5 mm 3 /N.m). For all test conditions, zirconium-lithium glass-ceramic revealed higher wear resistance and lower friction coefficient when compared to the polymer-infiltrated ceramic network. The findings indicated a less damage of glass-ceramics in comparison to polymer-matrix composites on sliding loading or micro-scale abrasion that can occur during the masticatory process.

Biotribology, 2020

The main aim of this study was to perform a scoping review on the wear behaviour of recent dental... more The main aim of this study was to perform a scoping review on the wear behaviour of recent dental glassceramics and its influence on the damage of opposing tooth enamel surfaces. Relevant in vivo and in vitro studies reported significant damage on enamel surfaces after friction against current glass-ceramics and therefore different parameters have been studied such as the glass-ceramic type, wear set up, environment, and surface treatment. The opposing enamel loss in volume, weight, or vertical measurement shown by previous studies is significantly higher when compared to enamel-by-enamel wear values. In fact, high values of hardness and elastic modulus of glass-ceramics combined with rough surfaces can result in detrimental effects on the tooth enamel surfaces. Restorative dental materials must withstand masticatory loads in combination with aesthetic benefits and high biocompatibility. Nevertheless, elastic modulus, hardness, and roughness of glass-ceramics should be adjusted to decrease the damage on tooth enamel surfaces.

Aquatic Toxicology, 2019

Highlights  Mytilus galloprovincialis bioaccumulated lanthanum  Mussels exposed to Lanthanum de... more Highlights  Mytilus galloprovincialis bioaccumulated lanthanum  Mussels exposed to Lanthanum decreased their metabolic capacity  Contaminated mussels activated their antioxidant and biotransformation defences  Contaminated mussels showed increased lipid peroxidation and lower GSH/GSSG ratio  Neurotoxicity was induced in contaminated mussels  Histopathological alterations were induced by lanthanum Summary Inappropriate processing and disposal of electronic waste contributes to the contamination of aquatic systems by various types of pollutants such as the rare-earth elements (REE) in which lanthanum (La) is included. Knowledge on the toxicity of these elements in marine organisms is still scarce when compared to other metals such as mercury (Hg) and arsenic (As).

Resources, 2019

Calcium carbonate is one of the most used raw materials in various industries, such as constructi... more Calcium carbonate is one of the most used raw materials in various industries, such as construction materials, food supplement, pharmaceutics, animal feed, plastic production, and others. Calcium carbonate can derive from marine wastes, like crustaceans and bivalve’s shells. The worldwide demand for new sources of food has increased exponentially, and following that tendency, the mariculture—especially the oyster culture—has been increasingly resorting to farming techniques. In 2016, 438 billion tons of oysters were produced. The majority of the shells were unduly discarded, presenting a public health problem. This article offers a solution based on the reuse and recycling of oyster shell residues in the production region of Florianópolis, SC, Brazil. The presented solution is an oyster shell by-product developed by a local company which produces artificial stone. The main component of the artificial stone is a composite material made of oyster shells incorporated in a polymeric res...

Journal of biomedical materials research. Part B, Applied biomaterials, Jan 2, 2018

The aim of this study was to conduct a literature review on the potential benefits of custom-made... more The aim of this study was to conduct a literature review on the potential benefits of custom-made root-analogue zirconia implants. A PubMed and ScienceDirect bibliographical search was carried out from 1969 to 2017. The increased interest in zirconia-based dental structures linked to aesthetic and biological outcomes have been reported in literature. Recent technological advances have focused on novel strategies for modification of zirconia-based surfaces to accelerate osseointegration. However, only a few studies revealed mechanical and biological benefits of custom-made root-analogue zirconia implants and therefore further studies should investigate the influence of different design and surface modification on the performance of such implants. Custom-made root-analogue zirconia implants have become a viable alternative to overcome limitations concerning stress distribution, aesthetics, and peri-implantitis induced by biofilms. However, further in vitro and in vivo studies on surfa...

Journal of hazardous materials, Jan 15, 2018

The experiments performed in this work proved the ability of Gracilaria gracilis to concentrate a... more The experiments performed in this work proved the ability of Gracilaria gracilis to concentrate and recover Critical Rare Elements (CRE) from contaminated waters. The importance of recycling these elements is related to their very limited sources in Nature and progressive use in technologies. Moreover, their mining exploitation has negative environmental impact, and recent studies point them as new emerging pollutants. To the best of our knowledge, this is the first report on the application of living macroalgae for the removal and recovery of CRE. G. gracilis (2.5gL, fresh weight) was exposed to mono- and multi-element saline solutions of 500μgL of Y, Ce, Nd, Eu and La. Removal was up to 70% in 48h, with bioaccumulation following Elovich kinetic model. In multi-element solutions, selectivity was not observed although removal of lanthanides improved comparatively to single-element solutions. No mortality or adverse effect on growth was registered. The subsequent macroalgae digestion...

Ecotoxicology and environmental safety, 2018

Organisms in marine systems are exposed to multiple stressors that create a range of associated e... more Organisms in marine systems are exposed to multiple stressors that create a range of associated environmental and ecotoxicological risks. Examples of stressors include alterations related to climate change, such as temperature increase, and the exposure to pollutants arising from human activities. The present study evaluated the impacts of Arsenic exposure (1mg/L) and warming (21°C) in Mytilus galloprovincialis, acting alone and in combination. Our results demonstrated that both Arsenic exposure and warming induced oxidative stress and reduced mussels metabolism, with changes becoming more prominent with the exposure time and when mussels were exposed to both stressors in combination. Furthermore, results obtained showed higher As accumulation in organisms exposed to warming treatments. The present study showed that under warming scenarios, the negative impacts induced by As may be enhanced in ecologically and economically relevant bivalves, with potential impacts on population stoc...

Journal of dentistry, Jan 31, 2017

Thermal cycling is widely used to simulate the aging of restorative materials corresponding to th... more Thermal cycling is widely used to simulate the aging of restorative materials corresponding to the changes of temperature in the oral cavity. However, test parameters present in literature vary considerably, which prevents comparison between different reports. The aim of this work is to assess the influence of the specimens' geometry and materials on the thermal stresses developed during thermal cycling tests. Finite elements method was used to simulate the conditions of thermal cycling tests for three different sample geometries: a three-points bending test sample, a cylinder rod and more complex shape of a restoration crown. Two different restorative systems were considered: all-ceramic (zirconia coupled with porcelain) and metal-ceramic (CoCrMo alloy coupled with porcelain). The stress state of each sample was evaluated throughout the test cycle. The results show that the sample geometry has great influence on the stress state, with difference of up to 230% in the maximum str...

Materials science & engineering. C, Materials for biological applications, 2017

PEEK is a promising polymer possessing high mechanical strength and biocompatibility and therefor... more PEEK is a promising polymer possessing high mechanical strength and biocompatibility and therefore it can be associated to titanium for biomedical applications. This study aimed at producing Ti6Al4V-PEEK joints with enhanced adhesion through laser-structuring Ti6Al4V treatments. Ti6Al4V cylindrical substrates were prepared by two types of surface treatments: alumina blasting and laser structuring. The holes number and size in laser-structured surfaces was varied. PEEK was then hot pressed against the metallic substrate to completely filling the surface cavities. The adhesion of the PEEK/Ti6Al4V joint was assessed by a shear bond strength test. Fracture surfaces and interfaces were investigated by SEM/EDS. Significant differences were found in the shear bond strength between alumina blasted and laser-structured samples. Bond strength improvement (exceeding 300%) was registered for the laser-structured specimens relative to grit-blasted ones. The laser-structuring technique showed to ...

Ceramics International, 2016

The purpose of this study was to evaluate the biaxial flexural stresses in classic bilayered and ... more The purpose of this study was to evaluate the biaxial flexural stresses in classic bilayered and in graded zirconia-feldspathic porcelain composites. A finite element method and an analytical model were used to simulate the piston-on-ring test and to predict the biaxial stress distributions across the thickness of the bilayer and graded zirconia-feldspathic porcelain discs. An axisymmetric model and a flexure formula of Hsueh et al. were used in the FEM and analytical analysis, respectively. Four porcelain thicknesses were tested in the bilayered discs. In graded discs, continuous and stepwise transitions from the bottom zirconia layer to the top porcelain layer were studied. The resulting stresses across the thickness, measured along the central axis of the disc, for the bilayered and graded discs were compared. In bilayered discs, the maximum tensile stress decreased while the stress mismatch (at the interface) increased with the porcelain layer thickness. The optimized balance between both variables is achieved for a porcelain thickness ratio in the range of 0.30-0.35. In graded discs, the highest tensile stresses were registered for porcelain rich interlayers (p=0.25) whereas the zirconia rich ones (p=8) yield the lowest tensile stresses. In addition, the maximum stresses in a graded structure can be tailored by altering compositional gradients. A decrease in maximum stresses with increasing values of p (a scaling exponent in the power law function) was observed. Our findings showed a good agreement between the analytical and simulated models, particularly in the tensile region of the disc. Graded zirconia-feldspathic porcelain composites exhibited a more favourable stress distribution relative to conventional bilayered systems. This fact can significantly impact the clinical performance of zirconiafeldspathic porcelain prostheses, namely reducing the fracture incidence of zirconia and the chipping and delamination of porcelain.

The Open Dentistry Journal, 2016

Background:Glass-ionomer cements perform a protective effect on the dentin-pulp complex consideri... more Background:Glass-ionomer cements perform a protective effect on the dentin-pulp complex considering the F ions release and chemical bonding to the dental structures. On the other hand, those materials have poor physic-mechanical properties in comparison with the restorative resin composite. The main aim of this work was to evaluate the influence of zirconia and/or alumina fillers on the microstructure and strength of a resin modified glass-ionomer cement after thermal cycling.Methods:Anin vitroexperimental study was carried out on 9 groups (n= 10) of cylindrical samples (6 x 4 mm) made from resin modified glass-ionomer (Vitremer, 3M, USA) with different contents of alumina and/or zirconia fillers. A nano-hybrid resin composite was tested as a control group. Samples were mechanically characterized by axial compressive tests and electron scanning microscopy (SEM) coupled to energy dispersive X-ray spectrophotometry (EDS), before and after thermal cycling. Thermal cycling procedures we...

Journal of the Mechanical Behavior of Biomedical Materials, 2016

In dentistry, prosthetic structures must be able to support masticatory loads combined with a hig... more In dentistry, prosthetic structures must be able to support masticatory loads combined with a high biocompatibility and wear resistance in the presence of a corrosive environment. In order to improve the simultaneous wear and corrosion response of highly biocompatible prosthetic structures, a veneering poly-ether-ether-ketone (PEEK) to Ti6Al4V substrate was assessed by tribocorrosion analyses under conditions mimicking the oral environment. Samples were synthesized by hot pressing the PEEK veneer onto Ti6Al4V cylinders. The tribocorrosion tests on Ti6Al4V or PEEK/Ti6Al4V samples were performed on a reciprocating ball-on-plate tribometer at 30N normal load, 1Hz and stroke length of 3mm. The tests were carried out in artificial saliva at 37°C. Open circuit potential (OCP) was measured before, during and after reciprocating sliding tests. The worn surfaces were characterized by scanning electron microscopy. The results revealed a lower wear rate on PEEK combined with a lower coefficient of friction (COF), when compared to Ti6Al4V. In fact, PEEK protected Ti6Al4V substrate against the corrosive environment and wear avoiding the release of metallic ions to the surrounding environment.