Buse çevik - Academia.edu (original) (raw)
Papers by Buse çevik
Journal of Photochemistry and Photobiology B: Biology
2020 Medical Technologies Congress (TIPTEKNO)
Photobiomodulation (PBM) is defined as the use of non-ionizing photonic energy to trigger photoch... more Photobiomodulation (PBM) is defined as the use of non-ionizing photonic energy to trigger photochemical changes, particularly in mitochondrial-sensitive cellular structures. Photobiomodulation is a form of treatment used in medicine in a practical and noninvasive way and it has a significant role in inflammation, ache, and pain reduction, wound healing, and tissue regeneration. It triggers proliferation and the activity of the cell, primarily by utilizing light from the near infrared-red to visible wavelength of the light (630-1000 nm). This in vitro study has analyzed comparatively the most appropriate energy doses with the wavelengths in the red and near-infrared spectrum to induce photobiomodulation on the keratinocyte cells. 1, 3, and 5mathrmJ/mathrmm25\mathrm{J}/ \mathrm{m}^{2}5mathrmJ/mathrmm2 energy densities of 655 nm and 808 nm diode lasers were used, which might affect wound healing mechanism and cell proliferation. The potential stimulating effect of photobiomodulation to promote wound healing and cell proliferation on human keratinocyte cells was analyzed via microscopic imaging of cell morphology, MTT analysis for cell proliferation and scratch assay for wound closure after light applications. The highest increase in cell viability was obtained with a rate of 112.6% after the triple treatment of 655-nm wavelength at 1 J/cm2. The best wound closure was achieved with a rate of 45% after the triple treatment of 655 nm wavelength at 3 J/cm2. This study revealed that PBM with 655-nm of wavelength was an effective tool to induce cell proliferation and speed up the wound healing process with specific energy doses.
Journal of Intelligent Systems with Applications, 2021
Photobiomodulation is a practical and noninvasive treatment that triggers cell proliferation, cel... more Photobiomodulation is a practical and noninvasive treatment that triggers cell proliferation, cell differentiation, wound healing, new tissue formation, inflammation and pain reduction with low-level light therapy. Light-emitting diodes (LEDs) are energy-saving, affordable and safe alternatives to laser devices which are recently preferred in photobiomodulation. Although the wavelengths between 600-700 nm are most preferred ones, there is a lack of practical optical systems which study this mechanism in vitro with different wavelengths simultaneously. In this study, a portable and remotely controlled multicolor LED-based system was designed and tested on the wound healing process of human keratinocytes by irradiating the cells homogenously with 3 different wavelengths (460-475 nm as blue, 515-535 nm as green, and 585-595 nm as orange) on different experimental groups at the same time. Its proliferative and wound healing effect was evaluated with cell viability (MTT) analysis and cel...
Photodermatology, Photoimmunology & Photomedicine, 2020
Background: Photobiomodulation depends on the use of non-ionizing light energy to trigger photoch... more Background: Photobiomodulation depends on the use of non-ionizing light energy to trigger photochemical changes, particularly in light-sensitive mitochondrial structures. It triggers proliferation and the metabolic activity of the cells, primarily by utilizing the energy from the near-infrared to the red wavelength of the light. Purpose: This in vitro study has analyzed comparatively the most appropriate energy doses and wavelengths to induce photobiomodulation on keratinocytes and fibroblasts for the accelerated wound healing process. Methods: 1, 3, and 5 J/cm² energy densities of 655-nm and 808-nm diode lasers were used to promote cell proliferation and wound healing process. Scratch assay and MTT analysis were performed on keratinocytes and fibroblasts for wound closure and cell proliferation after the triple light applications, respectively. Results: 655-nm of wavelength was more successful on keratinocytes to induce wound healing and cell proliferation, whereas 808-nm of wavelength was so effective on fibroblasts to heal the wounds totally and it induced cell proliferation almost 3 times compared to the untreated control group. Conclusion: This study revealed that photobiomodulation with 655 and 808 nm of wavelengths was effective to speed up the wound healing process at specific energy densities. In general 808nm of wavelength was more successful. However, the proper wavelength and the energy density may differ according to the cell type. Thus, every light parameter should be chosen properly to obtain better outcomes during photobiomodulation applications.
Photobiomodulation is a practical and non-invasive treatment that triggers cell proliferation, ce... more Photobiomodulation is a practical and non-invasive treatment that triggers cell proliferation, cell differentiation, wound healing, new tissue formation, inflammation and pain reduction with low-level light therapy. Light-emitting diodes (LEDs) are energy-saving, affordable and safe alternatives to laser devices which are recently preferred in photobiomodulation. Although the wavelengths between 600–700 nm are most preferred ones, there is a lack of practical optical systems which study this mechanism in vitro with different wavelengths simultaneously. In this study, a portable and remotely controlled multicolor LED-based system was designed and tested on the wound healing process of human keratinocytes by irradiating the cells homogenously with 3 different wavelengths (460–475 nm as blue, 515–535 nm as green, and 585–595 nm as orange) on different experimental groups at the same time. Its proliferative and wound healing effect was evaluated with cell viability (MTT) analysis and ce...
2021 Medical Technologies Congress (TIPTEKNO), 2021
Low level laser therapy (LLLT) is a photo therapy which is used in bone damages. LLLT increases c... more Low level laser therapy (LLLT) is a photo therapy which is used in bone damages. LLLT increases cell proliferation, stimulates osteogenesis and induces bone healing. Main mechanism of osteogenic differentiation via LLLT may depend on ATP level. Effects of 655 nm and 808 nm lasers were evaluated by using wound healing process in vitro monolayer system. Moreover, LLLT based ATP productions were evaluated. ATP levels of 655 nm and 808 nm lasers with 1J/cm2, 3J/cm2 and 5 J/cm2 energy dosages were compared with monolayer and 3D SFMs. Therefore, the study aimed that ATP level comparison between monolayer and SFMs. It contributed to enlighten ATP mechanisms of LLLT during HUVEC:hBMSC co-culture.
2020 Medical Technologies Congress (TIPTEKNO), 2020
Breast cancer is a fatal disease, and it is one of the most common cancer types among women in th... more Breast cancer is a fatal disease, and it is one of the most common cancer types among women in the world. The traditional monolayer methods are used to treat diseases. However, the method is limited in terms of the cell to cell communication and responses of cells to drugs. One of the main goals of cancer treatments is to prevent tumor metastasis and prevent diffusion in various areas of the body, thereby it is needed to increase the effectiveness of the treatments and reduce side effects. Peptides can be used in cancer treatment. Most peptide studies are performed in monolayer culture. These cultures can not accurately represent the complex intercellular and intracellular environment in clinical studies. Peptide studies must be performed in scaffold-free conditions to mimic the natural responses of cells. The study has been performed as scaffold-free microtissues with different NGR peptide concentrations. Results have been evaluated in terms of diameters, and viability of microtissues. It is concluded that 2 mM NGR is the most effective concentration in MCF-7 microtissue treatment.
2021 Medical Technologies Congress (TIPTEKNO), 2021
In this study, the effects of laminin-derived peptides on hBMSC-HUVEC Scaffold Free Microtissues ... more In this study, the effects of laminin-derived peptides on hBMSC-HUVEC Scaffold Free Microtissues (SFMs) were investigated. For this purpose, IKVAV, GRGDS and YIGSR peptides were synthesized in solid phase. Microwells were created by using agarose. SFMs were incubated with 0.5 mM, 1 mM and 2 mM laminin-derived peptides media. Experimental groups were compared with negative control which doesnt include any serum and peptide. On the 1st, 4th and 7th days, images were taken under the microscope and diameter analyzes were performed. Image-J program was used for dimensional analysis. Cell Viability Assay was used for viability analysis of SFMs. In terms of the viability and diameter analysis, the highest values of laminin-derived peptides were observed as 0.5 mM YIGSR, 1 mM IKVAV, and 2 mM GRGDS groups.
Soft Matter, 2021
Laminin derived peptide integrated self-assembled peptide hydrogels significantly enhanced vascul... more Laminin derived peptide integrated self-assembled peptide hydrogels significantly enhanced vascularisation of human umbilical vein endothelial cells (HUVECs).
Journal of Photochemistry and Photobiology B: Biology
2020 Medical Technologies Congress (TIPTEKNO)
Photobiomodulation (PBM) is defined as the use of non-ionizing photonic energy to trigger photoch... more Photobiomodulation (PBM) is defined as the use of non-ionizing photonic energy to trigger photochemical changes, particularly in mitochondrial-sensitive cellular structures. Photobiomodulation is a form of treatment used in medicine in a practical and noninvasive way and it has a significant role in inflammation, ache, and pain reduction, wound healing, and tissue regeneration. It triggers proliferation and the activity of the cell, primarily by utilizing light from the near infrared-red to visible wavelength of the light (630-1000 nm). This in vitro study has analyzed comparatively the most appropriate energy doses with the wavelengths in the red and near-infrared spectrum to induce photobiomodulation on the keratinocyte cells. 1, 3, and 5mathrmJ/mathrmm25\mathrm{J}/ \mathrm{m}^{2}5mathrmJ/mathrmm2 energy densities of 655 nm and 808 nm diode lasers were used, which might affect wound healing mechanism and cell proliferation. The potential stimulating effect of photobiomodulation to promote wound healing and cell proliferation on human keratinocyte cells was analyzed via microscopic imaging of cell morphology, MTT analysis for cell proliferation and scratch assay for wound closure after light applications. The highest increase in cell viability was obtained with a rate of 112.6% after the triple treatment of 655-nm wavelength at 1 J/cm2. The best wound closure was achieved with a rate of 45% after the triple treatment of 655 nm wavelength at 3 J/cm2. This study revealed that PBM with 655-nm of wavelength was an effective tool to induce cell proliferation and speed up the wound healing process with specific energy doses.
Journal of Intelligent Systems with Applications, 2021
Photobiomodulation is a practical and noninvasive treatment that triggers cell proliferation, cel... more Photobiomodulation is a practical and noninvasive treatment that triggers cell proliferation, cell differentiation, wound healing, new tissue formation, inflammation and pain reduction with low-level light therapy. Light-emitting diodes (LEDs) are energy-saving, affordable and safe alternatives to laser devices which are recently preferred in photobiomodulation. Although the wavelengths between 600-700 nm are most preferred ones, there is a lack of practical optical systems which study this mechanism in vitro with different wavelengths simultaneously. In this study, a portable and remotely controlled multicolor LED-based system was designed and tested on the wound healing process of human keratinocytes by irradiating the cells homogenously with 3 different wavelengths (460-475 nm as blue, 515-535 nm as green, and 585-595 nm as orange) on different experimental groups at the same time. Its proliferative and wound healing effect was evaluated with cell viability (MTT) analysis and cel...
Photodermatology, Photoimmunology & Photomedicine, 2020
Background: Photobiomodulation depends on the use of non-ionizing light energy to trigger photoch... more Background: Photobiomodulation depends on the use of non-ionizing light energy to trigger photochemical changes, particularly in light-sensitive mitochondrial structures. It triggers proliferation and the metabolic activity of the cells, primarily by utilizing the energy from the near-infrared to the red wavelength of the light. Purpose: This in vitro study has analyzed comparatively the most appropriate energy doses and wavelengths to induce photobiomodulation on keratinocytes and fibroblasts for the accelerated wound healing process. Methods: 1, 3, and 5 J/cm² energy densities of 655-nm and 808-nm diode lasers were used to promote cell proliferation and wound healing process. Scratch assay and MTT analysis were performed on keratinocytes and fibroblasts for wound closure and cell proliferation after the triple light applications, respectively. Results: 655-nm of wavelength was more successful on keratinocytes to induce wound healing and cell proliferation, whereas 808-nm of wavelength was so effective on fibroblasts to heal the wounds totally and it induced cell proliferation almost 3 times compared to the untreated control group. Conclusion: This study revealed that photobiomodulation with 655 and 808 nm of wavelengths was effective to speed up the wound healing process at specific energy densities. In general 808nm of wavelength was more successful. However, the proper wavelength and the energy density may differ according to the cell type. Thus, every light parameter should be chosen properly to obtain better outcomes during photobiomodulation applications.
Photobiomodulation is a practical and non-invasive treatment that triggers cell proliferation, ce... more Photobiomodulation is a practical and non-invasive treatment that triggers cell proliferation, cell differentiation, wound healing, new tissue formation, inflammation and pain reduction with low-level light therapy. Light-emitting diodes (LEDs) are energy-saving, affordable and safe alternatives to laser devices which are recently preferred in photobiomodulation. Although the wavelengths between 600–700 nm are most preferred ones, there is a lack of practical optical systems which study this mechanism in vitro with different wavelengths simultaneously. In this study, a portable and remotely controlled multicolor LED-based system was designed and tested on the wound healing process of human keratinocytes by irradiating the cells homogenously with 3 different wavelengths (460–475 nm as blue, 515–535 nm as green, and 585–595 nm as orange) on different experimental groups at the same time. Its proliferative and wound healing effect was evaluated with cell viability (MTT) analysis and ce...
2021 Medical Technologies Congress (TIPTEKNO), 2021
Low level laser therapy (LLLT) is a photo therapy which is used in bone damages. LLLT increases c... more Low level laser therapy (LLLT) is a photo therapy which is used in bone damages. LLLT increases cell proliferation, stimulates osteogenesis and induces bone healing. Main mechanism of osteogenic differentiation via LLLT may depend on ATP level. Effects of 655 nm and 808 nm lasers were evaluated by using wound healing process in vitro monolayer system. Moreover, LLLT based ATP productions were evaluated. ATP levels of 655 nm and 808 nm lasers with 1J/cm2, 3J/cm2 and 5 J/cm2 energy dosages were compared with monolayer and 3D SFMs. Therefore, the study aimed that ATP level comparison between monolayer and SFMs. It contributed to enlighten ATP mechanisms of LLLT during HUVEC:hBMSC co-culture.
2020 Medical Technologies Congress (TIPTEKNO), 2020
Breast cancer is a fatal disease, and it is one of the most common cancer types among women in th... more Breast cancer is a fatal disease, and it is one of the most common cancer types among women in the world. The traditional monolayer methods are used to treat diseases. However, the method is limited in terms of the cell to cell communication and responses of cells to drugs. One of the main goals of cancer treatments is to prevent tumor metastasis and prevent diffusion in various areas of the body, thereby it is needed to increase the effectiveness of the treatments and reduce side effects. Peptides can be used in cancer treatment. Most peptide studies are performed in monolayer culture. These cultures can not accurately represent the complex intercellular and intracellular environment in clinical studies. Peptide studies must be performed in scaffold-free conditions to mimic the natural responses of cells. The study has been performed as scaffold-free microtissues with different NGR peptide concentrations. Results have been evaluated in terms of diameters, and viability of microtissues. It is concluded that 2 mM NGR is the most effective concentration in MCF-7 microtissue treatment.
2021 Medical Technologies Congress (TIPTEKNO), 2021
In this study, the effects of laminin-derived peptides on hBMSC-HUVEC Scaffold Free Microtissues ... more In this study, the effects of laminin-derived peptides on hBMSC-HUVEC Scaffold Free Microtissues (SFMs) were investigated. For this purpose, IKVAV, GRGDS and YIGSR peptides were synthesized in solid phase. Microwells were created by using agarose. SFMs were incubated with 0.5 mM, 1 mM and 2 mM laminin-derived peptides media. Experimental groups were compared with negative control which doesnt include any serum and peptide. On the 1st, 4th and 7th days, images were taken under the microscope and diameter analyzes were performed. Image-J program was used for dimensional analysis. Cell Viability Assay was used for viability analysis of SFMs. In terms of the viability and diameter analysis, the highest values of laminin-derived peptides were observed as 0.5 mM YIGSR, 1 mM IKVAV, and 2 mM GRGDS groups.
Soft Matter, 2021
Laminin derived peptide integrated self-assembled peptide hydrogels significantly enhanced vascul... more Laminin derived peptide integrated self-assembled peptide hydrogels significantly enhanced vascularisation of human umbilical vein endothelial cells (HUVECs).