César Vásquez - Academia.edu (original) (raw)
Papers by César Vásquez
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1984
ABSTRACT 1.1. A brief survey of some of the properties of plasmids isolated from thermophilic bac... more ABSTRACT 1.1. A brief survey of some of the properties of plasmids isolated from thermophilic bacteria is presented.2.2. The utility of these extrachromosomal elements as models to study the structure and organization of DNA in these microorganisms is analyzed.3.3. Their use as vectors for high temperature cloning systems is discussed.
Febs Letters, 1983
and keywords not received
Experimental and Molecular Pathology, 1965
Kilham's rat virus has been purified and studied with the negative stain method. Its ultrastrutur... more Kilham's rat virus has been purified and studied with the negative stain method. Its ultrastrutural analysis is discussed and an interpretation of its shape and symmetry is tentatively proposed. The virus was found to have a mean diameter of 180 Å. It has an angular profile and seems to be roughly hexagonal, with concavities and protuberances. The number of peripherical capsomeres does not exceed 10. The findings can be explained with a model representing a pentagonal dodecahedron. The capsomeres measure approximately 30 Å in length and 20 Å in width. Their number is likely to be 32, thus forming a protective coat with very small gaps.
Experimental and Molecular Pathology, 1965
... EXPERIMENTAL AND MOLECULAR PATHOLOGY 4, 130-140 (1965) Purification and Fine Structure of Kil... more ... EXPERIMENTAL AND MOLECULAR PATHOLOGY 4, 130-140 (1965) Purification and Fine Structure of Kilham's Rat Virus CESAR VASQUEZ AND CARLOS BRAILOVSKY Institut de Recherches sur le Cancer, Villejuif (Seine), France Received October 12, 1964 The Kilham rat ...
The Journal of Investigative Dermatology publishes basic and clinical research in cutaneous biolo... more The Journal of Investigative Dermatology publishes basic and clinical research in cutaneous biology and skin disease.
Journal of Molecular Biology, 1969
Twisted DNA rings were released from polyoma virus particles upon spreading on a protein film ove... more Twisted DNA rings were released from polyoma virus particles upon spreading on a protein film over a denaturing hypophase of urea and sodium perchlorate. Conformational changes were observed while the DNA molecules were irreversibly adsorbed to the monolayer. In our preparations, 80% of the DNA rings fist appeared as twisted molecules and 15% as open rings, before all molecules in the monolayer were slowly denatured to tightly coiled forms. 5% were linear forms.
Journal of Membrane Biology, 1971
A special technique for the electron-microscope study of the fine structure of ultrathin artifici... more A special technique for the electron-microscope study of the fine structure of ultrathin artificial membranes is described. Membranes made of total phospholipids of the cerebral cortex and cholesterol showed globular elements of 40 Å embedded in a denser and diffuse matrix. These same elements were also seen organized in a periodic banded pattern. Identical patterns were observed with and without supporting films. Lipidic membranes containing small amounts of proteolipid fromElectrophorus showed a lower electron density, a finer and smoother texture and a decrease in electrical resistance. Lipidic membranes containing the cholinergic receptor proteolipid fromElectrophorus, upon addition of acetylcholine, showed a rapid and transient rise in conductance which was accompanied by changes in fine structure, consisting in a more uneven corrugated appearance of the membrane and the presence of dense spots of 20 Å. These results are discussed in relation to the channel hypothesis of ion permeability. It is postulated that the binding of acetylcholine by the receptor proteolipid results in conformational changes in this protein that facilitate the translocation of ions through the membrane.
Journal of Molecular Biology, 1970
Electron micrographs have been obtained of proteolipid macromolecules from cerebral cortex during... more Electron micrographs have been obtained of proteolipid macromolecules from cerebral cortex during the successive steps of extraction and purification. These macromolecules were seen as short filaments having a width of about 15 A and a minimum length of 150 li and showed a high tendency to aggregate. This study was mainly concentrated on the "receptor proteolipid" which, by previous studies, was shown to have high affinity binding for different drugs active in nerve transmission. The binding of atropine sulphate to the proteolipid macromolecules was followed and different patterns of organization were observed. Ordered arrays of fibres, forming tubule-like structures were found with low concentrations of the drug. Then, as the concentration of atropine sulphate increased a drastic change occurred consisting of aggregation and the formation of amorphous clusters of proteolipid macromolecules. 221 222 C. VASQUEZ ET AL.
Journal of Molecular Biology, 1968
A gradual release of double-stranded RNA occurred after the spreading of reovirus particles on ur... more A gradual release of double-stranded RNA occurred after the spreading of reovirus particles on urea in the cold. Electron microscopy of virus-protein monolayers showed that each virion expelled about 11 fragments of 8.3 μ, maximum total length. This gives an estimated RNA molecular weight between 17 and 22 million daltons per virion. The length distribution of the fragments was trimodal, with peaks at 0.38, 0.66 and 1.13 μ, but there was large variation of filament lengths in each peak.
THE DNA of the B. subtilis phage ϕ29 has been described as unpermuted linear duplex molecules1 of... more THE DNA of the B. subtilis phage ϕ29 has been described as unpermuted linear duplex molecules1 of molecular weight 11 × 106, but the formation of circular molecules has also been indicated, suggesting the existence of cohesive ends1,2.
Journal of cell science, 2014
Insulin signaling includes generation of low levels of H 2 O 2 ; however, its origin and contribu... more Insulin signaling includes generation of low levels of H 2 O 2 ; however, its origin and contribution to insulin-stimulated glucose transport are unknown. We tested the impact of H 2 O 2 on insulin-dependent glucose transport and GLUT4 translocation in skeletal muscle cells. H 2 O 2 increased GLUT4myc translocation, an effect additive to that of insulin. The anti-oxidants N-acetyl L-cysteine and Trolox, the p47 phox /NOX2 NADPH oxidase inhibitory peptide gp91-ds-tat or p47 phox knockdown each reduced insulin-dependent GLUT4myc translocation. Importantly, gp91-ds-tat suppressed insulin-dependent H 2 O 2 production. A ryanodine-receptor (RyR) channel agonist stimulated GLUT4myc translocation and insulin stimulated RyR1-mediated Ca 2+ release by promoting RyR1 Sglutathionylation.
Virology, 1979
Electron microscope images of negative-stained particles of FMDV have been analyzed. The viral ca... more Electron microscope images of negative-stained particles of FMDV have been analyzed. The viral capsid appeared to be a pliant structure composed of monomers having an average width of about 2.5 nm and a length of 4 nm. Upon capsid disruption, a sequential and ...
Virology, 1978
... Virology 69, 369377. DENOYA, CD, SCODELLER, EA, GiMENEZ, BH, VASQUEZ, C., and LATORRE, JL (19... more ... Virology 69, 369377. DENOYA, CD, SCODELLER, EA, GiMENEZ, BH, VASQUEZ, C., and LATORRE, JL (1978). Footand mouth disease virus. I Stability of its ribonucleic acid. Virology 84, 174179. ... 10, 202210. KAPLAN, JC, WILBERT, SM, and BLACK, PH (1972). ...
Virology, 1978
... Biophys. Acta. 262, 220226 (1972). 16. VASQUEZ, C., PALMA, EL, and BARRANTES, F. J,, J. Micro... more ... Biophys. Acta. 262, 220226 (1972). 16. VASQUEZ, C., PALMA, EL, and BARRANTES, F. J,, J. Microsc. 14, 147154 (1972). 17. ... 15111518. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (19). 24. JJ Pene, E. Knight, Jr and JE Darnell, Jr. J. Mol. Biol. ...
European Journal of Biochemistry, 1981
A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was ea... more A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was easily obtained using an RNA extraction procedure based on a mild deproteinization with Sarkosyl and cold phenol/ chloroform. This technique assured the recovery of several proteins and the endoribonuclease in association with the RNA.
Endocrinology, 2010
Intracellular calcium levels ([Ca 2ϩ ] i ) and glucose uptake are central to cardiomyocyte physio... more Intracellular calcium levels ([Ca 2ϩ ] i ) and glucose uptake are central to cardiomyocyte physiology, yet connections between them have not been studied. We investigated whether insulin regulates [Ca 2ϩ ] i in cultured cardiomyocytes, the participating mechanisms, and their influence on glucose uptake via SLC2 family of facilitative glucose transporter 4 (GLUT4).
European Journal of Biochemistry, 1981
A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was ea... more A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was easily obtained using an RNA extraction procedure based on a mild deproteinization with Sarkosyl and cold phenol/ chloroform. This technique assured the recovery of several proteins and the endoribonuclease in association with the RNA.
European Journal of Biochemistry, 1977
The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural prote... more The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural proteins has been studied. Mutants in three cistrons analyzed that are involved in phage DNA synthesis, as well as in cistron 16 which codes for a late nonstructural protein, produce prolate capsids which are more rounded at the corners than complete phage heads and have an internal core; they contain the head proteins, the upper collar protein and protein p7, not present in mature phage particles. Mutants in cistron 7 do not produce capsids nor other phage-related structures; this result and the presence of p7 in phage capsids suggest an essential role in capsid assembly for this protein. The protein product of cistron 13 is probably needed for a stable DNA encapsulation since mutants in this cistron produce mainly DNA-free complete phage particles and only about 10 % of uninfective DNA-containing complete phage. Cistron 15 codes for a late, partially dispensable, nonstructural protein which is present in the DNA-free capsids produced after infection with the delayed-lysis mutant sus14(1242), used as the wild-type control, or with mutants in cistrons 9, 11, 12 and 13. Proteins pl5 and p16 are probably involved in the encapsulation of viral DNA in a prohead.
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1984
ABSTRACT 1.1. A brief survey of some of the properties of plasmids isolated from thermophilic bac... more ABSTRACT 1.1. A brief survey of some of the properties of plasmids isolated from thermophilic bacteria is presented.2.2. The utility of these extrachromosomal elements as models to study the structure and organization of DNA in these microorganisms is analyzed.3.3. Their use as vectors for high temperature cloning systems is discussed.
Febs Letters, 1983
and keywords not received
Experimental and Molecular Pathology, 1965
Kilham's rat virus has been purified and studied with the negative stain method. Its ultrastrutur... more Kilham's rat virus has been purified and studied with the negative stain method. Its ultrastrutural analysis is discussed and an interpretation of its shape and symmetry is tentatively proposed. The virus was found to have a mean diameter of 180 Å. It has an angular profile and seems to be roughly hexagonal, with concavities and protuberances. The number of peripherical capsomeres does not exceed 10. The findings can be explained with a model representing a pentagonal dodecahedron. The capsomeres measure approximately 30 Å in length and 20 Å in width. Their number is likely to be 32, thus forming a protective coat with very small gaps.
Experimental and Molecular Pathology, 1965
... EXPERIMENTAL AND MOLECULAR PATHOLOGY 4, 130-140 (1965) Purification and Fine Structure of Kil... more ... EXPERIMENTAL AND MOLECULAR PATHOLOGY 4, 130-140 (1965) Purification and Fine Structure of Kilham's Rat Virus CESAR VASQUEZ AND CARLOS BRAILOVSKY Institut de Recherches sur le Cancer, Villejuif (Seine), France Received October 12, 1964 The Kilham rat ...
The Journal of Investigative Dermatology publishes basic and clinical research in cutaneous biolo... more The Journal of Investigative Dermatology publishes basic and clinical research in cutaneous biology and skin disease.
Journal of Molecular Biology, 1969
Twisted DNA rings were released from polyoma virus particles upon spreading on a protein film ove... more Twisted DNA rings were released from polyoma virus particles upon spreading on a protein film over a denaturing hypophase of urea and sodium perchlorate. Conformational changes were observed while the DNA molecules were irreversibly adsorbed to the monolayer. In our preparations, 80% of the DNA rings fist appeared as twisted molecules and 15% as open rings, before all molecules in the monolayer were slowly denatured to tightly coiled forms. 5% were linear forms.
Journal of Membrane Biology, 1971
A special technique for the electron-microscope study of the fine structure of ultrathin artifici... more A special technique for the electron-microscope study of the fine structure of ultrathin artificial membranes is described. Membranes made of total phospholipids of the cerebral cortex and cholesterol showed globular elements of 40 Å embedded in a denser and diffuse matrix. These same elements were also seen organized in a periodic banded pattern. Identical patterns were observed with and without supporting films. Lipidic membranes containing small amounts of proteolipid fromElectrophorus showed a lower electron density, a finer and smoother texture and a decrease in electrical resistance. Lipidic membranes containing the cholinergic receptor proteolipid fromElectrophorus, upon addition of acetylcholine, showed a rapid and transient rise in conductance which was accompanied by changes in fine structure, consisting in a more uneven corrugated appearance of the membrane and the presence of dense spots of 20 Å. These results are discussed in relation to the channel hypothesis of ion permeability. It is postulated that the binding of acetylcholine by the receptor proteolipid results in conformational changes in this protein that facilitate the translocation of ions through the membrane.
Journal of Molecular Biology, 1970
Electron micrographs have been obtained of proteolipid macromolecules from cerebral cortex during... more Electron micrographs have been obtained of proteolipid macromolecules from cerebral cortex during the successive steps of extraction and purification. These macromolecules were seen as short filaments having a width of about 15 A and a minimum length of 150 li and showed a high tendency to aggregate. This study was mainly concentrated on the "receptor proteolipid" which, by previous studies, was shown to have high affinity binding for different drugs active in nerve transmission. The binding of atropine sulphate to the proteolipid macromolecules was followed and different patterns of organization were observed. Ordered arrays of fibres, forming tubule-like structures were found with low concentrations of the drug. Then, as the concentration of atropine sulphate increased a drastic change occurred consisting of aggregation and the formation of amorphous clusters of proteolipid macromolecules. 221 222 C. VASQUEZ ET AL.
Journal of Molecular Biology, 1968
A gradual release of double-stranded RNA occurred after the spreading of reovirus particles on ur... more A gradual release of double-stranded RNA occurred after the spreading of reovirus particles on urea in the cold. Electron microscopy of virus-protein monolayers showed that each virion expelled about 11 fragments of 8.3 μ, maximum total length. This gives an estimated RNA molecular weight between 17 and 22 million daltons per virion. The length distribution of the fragments was trimodal, with peaks at 0.38, 0.66 and 1.13 μ, but there was large variation of filament lengths in each peak.
THE DNA of the B. subtilis phage ϕ29 has been described as unpermuted linear duplex molecules1 of... more THE DNA of the B. subtilis phage ϕ29 has been described as unpermuted linear duplex molecules1 of molecular weight 11 × 106, but the formation of circular molecules has also been indicated, suggesting the existence of cohesive ends1,2.
Journal of cell science, 2014
Insulin signaling includes generation of low levels of H 2 O 2 ; however, its origin and contribu... more Insulin signaling includes generation of low levels of H 2 O 2 ; however, its origin and contribution to insulin-stimulated glucose transport are unknown. We tested the impact of H 2 O 2 on insulin-dependent glucose transport and GLUT4 translocation in skeletal muscle cells. H 2 O 2 increased GLUT4myc translocation, an effect additive to that of insulin. The anti-oxidants N-acetyl L-cysteine and Trolox, the p47 phox /NOX2 NADPH oxidase inhibitory peptide gp91-ds-tat or p47 phox knockdown each reduced insulin-dependent GLUT4myc translocation. Importantly, gp91-ds-tat suppressed insulin-dependent H 2 O 2 production. A ryanodine-receptor (RyR) channel agonist stimulated GLUT4myc translocation and insulin stimulated RyR1-mediated Ca 2+ release by promoting RyR1 Sglutathionylation.
Virology, 1979
Electron microscope images of negative-stained particles of FMDV have been analyzed. The viral ca... more Electron microscope images of negative-stained particles of FMDV have been analyzed. The viral capsid appeared to be a pliant structure composed of monomers having an average width of about 2.5 nm and a length of 4 nm. Upon capsid disruption, a sequential and ...
Virology, 1978
... Virology 69, 369377. DENOYA, CD, SCODELLER, EA, GiMENEZ, BH, VASQUEZ, C., and LATORRE, JL (19... more ... Virology 69, 369377. DENOYA, CD, SCODELLER, EA, GiMENEZ, BH, VASQUEZ, C., and LATORRE, JL (1978). Footand mouth disease virus. I Stability of its ribonucleic acid. Virology 84, 174179. ... 10, 202210. KAPLAN, JC, WILBERT, SM, and BLACK, PH (1972). ...
Virology, 1978
... Biophys. Acta. 262, 220226 (1972). 16. VASQUEZ, C., PALMA, EL, and BARRANTES, F. J,, J. Micro... more ... Biophys. Acta. 262, 220226 (1972). 16. VASQUEZ, C., PALMA, EL, and BARRANTES, F. J,, J. Microsc. 14, 147154 (1972). 17. ... 15111518. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (19). 24. JJ Pene, E. Knight, Jr and JE Darnell, Jr. J. Mol. Biol. ...
European Journal of Biochemistry, 1981
A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was ea... more A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was easily obtained using an RNA extraction procedure based on a mild deproteinization with Sarkosyl and cold phenol/ chloroform. This technique assured the recovery of several proteins and the endoribonuclease in association with the RNA.
Endocrinology, 2010
Intracellular calcium levels ([Ca 2ϩ ] i ) and glucose uptake are central to cardiomyocyte physio... more Intracellular calcium levels ([Ca 2ϩ ] i ) and glucose uptake are central to cardiomyocyte physiology, yet connections between them have not been studied. We investigated whether insulin regulates [Ca 2ϩ ] i in cultured cardiomyocytes, the participating mechanisms, and their influence on glucose uptake via SLC2 family of facilitative glucose transporter 4 (GLUT4).
European Journal of Biochemistry, 1981
A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was ea... more A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was easily obtained using an RNA extraction procedure based on a mild deproteinization with Sarkosyl and cold phenol/ chloroform. This technique assured the recovery of several proteins and the endoribonuclease in association with the RNA.
European Journal of Biochemistry, 1977
The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural prote... more The effect on phage morphogenesis of sus mutations in the cistrons coding for nonstructural proteins has been studied. Mutants in three cistrons analyzed that are involved in phage DNA synthesis, as well as in cistron 16 which codes for a late nonstructural protein, produce prolate capsids which are more rounded at the corners than complete phage heads and have an internal core; they contain the head proteins, the upper collar protein and protein p7, not present in mature phage particles. Mutants in cistron 7 do not produce capsids nor other phage-related structures; this result and the presence of p7 in phage capsids suggest an essential role in capsid assembly for this protein. The protein product of cistron 13 is probably needed for a stable DNA encapsulation since mutants in this cistron produce mainly DNA-free complete phage particles and only about 10 % of uninfective DNA-containing complete phage. Cistron 15 codes for a late, partially dispensable, nonstructural protein which is present in the DNA-free capsids produced after infection with the delayed-lysis mutant sus14(1242), used as the wild-type control, or with mutants in cistrons 9, 11, 12 and 13. Proteins pl5 and p16 are probably involved in the encapsulation of viral DNA in a prohead.