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Papers by Cresilda Alinapon

Research paper thumbnail of AtSRP1, SMALL RUBBER PARTICLE PROTEIN HOMOLOG, functions in pollen growth and development in Arabidopsis

Biochemical and Biophysical Research Communications, 2016

To identify novel roles of SMALL RUBBER PARTICLE PROTEIN Homolog in the nonrubber-producing plant... more To identify novel roles of SMALL RUBBER PARTICLE PROTEIN Homolog in the nonrubber-producing plant Arabidopsis (AtSRP1), we isolated a T-DNA-insertion knockout mutant (FLAG_543A05) and investigated its functional characteristics. AtSRP1 is predominantly expressed in reproductive organs and is localized to lipid droplets and ER. Compared to wild-type (WT) Arabidopsis, atsrp1 plants contain small siliques with a reduced number of heterogeneously shaped seeds. The size of anther and pollen grains in atsrp1 is highly irregular, with a lower grain number than WT. Therefore, AtSRP1 plays a novel role related to pollen growth and development in a non-rubber-producing plant.

Research paper thumbnail of The Plasmodiophora brassicae Golgi-localized UPF0016 protein PbGDT1 mediates calcium but not manganese transport in yeast and Nicotiana benthamiana

Fungal genetics and biology, Apr 1, 2024

Research paper thumbnail of Essential Macro-Minerals, Crude Protein and Total Antioxidant Activity of Powdered Ginger and Turmeric at Varied Drying Methods

Central Mindanao University Journal of Science

Drying is widely usedd and one of the most effective methods for food preservation. This study re... more Drying is widely usedd and one of the most effective methods for food preservation. This study reports the macro-mineral, crude protein, and antioxidant activity of locally produced powdered ginger and turmeric using the air-, oven- and sun drying methods. Kjeldahl method, atomic absorption spectroscopy, and phosphomolybdenum method were used to determine the crude protein, macrominerals (K, Ca, and Mg), and total antioxidant activity (TAA), respectively. Highest TAA was observed in sun-dried turmeric (7.82 ± 4.97mg AAE/g dried sample) while the lowest TAA was observed in sun-dried ginger powder (2.14 ± 0.31 mg AAE/mg dried sample). The measured crude protein of turmeric ranged from 8.34 ± 0.93 - 8.54 ± 0.14% while 6.25 ± 0.42 - 7.41 ± 1.05% for ginger. Essential macro-minerals in turmeric and ginger at varied drying methods ranged from 0.07 to 2.21 % for K, 0.04 to 0.12 % for% for Mg, and 0.79 to 1.15 % for Ca. ANOVA revealed that the effects of drying methods were only significant...

Research paper thumbnail of Antioxidant and Cytotoxic Activity of the Leaf Ethanolic Extracts of Tithonia diversofilia and Gliricidia sepium from Bukidnon, Philippines

Asian Journal of Biological and Life Sciences, 2019

Introduction: Nature has been an attractive source of new therapeutic candidate compounds. The pl... more Introduction: Nature has been an attractive source of new therapeutic candidate compounds. The plants produce naturally occurring secondary metabolites which are being investigated for their anticancer and antioxidant activities. Tithonia diversifolia and Gliricidia sepium are commonly growing in Bukidnon, Philippines. These plants have long list of ethno-medicinal uses. Thus, there is a need to further investigate their phytochemical constituents as well as their biological activities. Objectives: The study focuses on the phytochemicals in the ethanolic leaf extracts of T. diversifolia and G. sepium collected from Bukidnon, Philippines. Moreover, cytotoxic and antioxidant properties of the extracts were also determined through Brine Shrimp Lethality Assay (BSLA) and Total Antioxidant Capacity, respectively. Materials and Methods: Qualitative phytochemical screening and determination of Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Antioxidant Capacity (TAC) and cytotoxicity of the ethanolic leaf extracts of T. diversifolia and G. sepium were conducted. Results and Discussion: Results of the study showed the presence of flavonoids, phenolics, tannins and terpenoids in the ethanolic leaf extracts of T. diversifolia and G. sepium. The TPC of T. diversifolia (15.20 mg GAE/g dry sample) was found higher than that of G. sepium (14.43 mg GAE/g dry sample). Similar trend was also observed in the TFC where T. diversifolia and G. sepium recorded 12.50 and 9.00 mg QE/g dry sample, respectively. T. diversifolia also gave higher TAC of 302.8 mg AAE/ g dry sample than G. sepium (200.2 mg AAE/g dry sample). For cytotoxicity test, T. diversifolia (LC 50 = 14.57 mg/L) exhibited more potent toxicity as compared to G. sepium (LC 50 = 15.85 mg/L). The results indicate that the detected phytochemicals may account for the exhibited biological activities of T. diversifolia and G. sepium. Conclusion: This study uncovers the promising antioxidative and cytotoxic property of G. sepium and T. diversifolia that warrants further investigation.

Research paper thumbnail of Cytotoxicity, Antioxidant Activity, and Total Phenolic Content of the Ethanolic Leaf Extract of Hornstedtia conoidea Ridl

Asian Journal of Biological and Life sciences

Research paper thumbnail of The membrane-tethered NAC transcription factor, AtNTL7, contributes to ER-stress resistance in Arabidopsis

Biochemical and biophysical research communications, Jul 12, 2017

We screened for endoplasmic reticulum (ER) stress-resistant mutants among 25 mutants of the Arabi... more We screened for endoplasmic reticulum (ER) stress-resistant mutants among 25 mutants of the Arabidopsis NTL (NAC with Transmembrane motif 1-Like) family. We identified a novel mutant, SALK_044777, showing strong resistance to ER stress. RT-PCR and genomic DNA sequence analyses identified the mutant as atntl7, which harbors a T-DNA insertion in the fourth exon of AtNTL7. Two other atntl7-mutant alleles, in which T-DNA was inserted in the second exon and third intron of AtNTL7, respectively, showed ER-stress sensitive phenotypes, suggesting that SALK_044777 is a gain-of-function mutant. Arabidopsis plants overexpressing AtNTL7 showed strong ER-stress resistance. Our findings suggest that AtNTL7 fragment is cleaved from the ER membrane under ER stress and translocates into the nucleus to induce downstream ER-stress responsive genes.

Research paper thumbnail of Antibacterial activity of the crude ethanolic extracts of Etlingera elatior and Etlingera philippinensis

Antibacterial, Etlingera elatior, Etlingera philippinensis

Research paper thumbnail of Universal Stress Protein Exhibits a Redox-Dependent Chaperone Function in Arabidopsis and Enhances Plant Tolerance to Heat Shock and Oxidative Stress

Frontiers in Plant Science, 2015

Although a wide range of physiological information on Universal Stress Proteins (USPs) is availab... more Although a wide range of physiological information on Universal Stress Proteins (USPs) is available from many organisms, their biochemical, and molecular functions remain unidentified. The biochemical function of AtUSP (At3g53990) from Arabidopsis thaliana was therefore investigated. Plants over-expressing AtUSP showed a strong resistance to heat shock and oxidative stress, compared with wild-type and Atusp knockout plants, confirming the crucial role of AtUSP in stress tolerance. AtUSP was present in a variety of structures including monomers, dimers, trimers, and oligomeric complexes, and switched in response to external stresses from low molecular weight (LMW) species to high molecular weight (HMW) complexes. AtUSP exhibited a strong chaperone function under stress conditions in particular, and this activity was significantly increased by heat treatment. Chaperone activity of AtUSP was critically regulated by the redox status of cells and accompanied by structural changes to the protein. Over-expression of AtUSP conferred a strong tolerance to heat shock and oxidative stress upon Arabidopsis, primarily via its chaperone function.

Research paper thumbnail of AtSRP1, SMALL RUBBER PARTICLE PROTEIN HOMOLOG, functions in pollen growth and development in Arabidopsis

Biochemical and Biophysical Research Communications, 2016

To identify novel roles of SMALL RUBBER PARTICLE PROTEIN Homolog in the nonrubber-producing plant... more To identify novel roles of SMALL RUBBER PARTICLE PROTEIN Homolog in the nonrubber-producing plant Arabidopsis (AtSRP1), we isolated a T-DNA-insertion knockout mutant (FLAG_543A05) and investigated its functional characteristics. AtSRP1 is predominantly expressed in reproductive organs and is localized to lipid droplets and ER. Compared to wild-type (WT) Arabidopsis, atsrp1 plants contain small siliques with a reduced number of heterogeneously shaped seeds. The size of anther and pollen grains in atsrp1 is highly irregular, with a lower grain number than WT. Therefore, AtSRP1 plays a novel role related to pollen growth and development in a non-rubber-producing plant.

Research paper thumbnail of The Plasmodiophora brassicae Golgi-localized UPF0016 protein PbGDT1 mediates calcium but not manganese transport in yeast and Nicotiana benthamiana

Fungal genetics and biology, Apr 1, 2024

Research paper thumbnail of Essential Macro-Minerals, Crude Protein and Total Antioxidant Activity of Powdered Ginger and Turmeric at Varied Drying Methods

Central Mindanao University Journal of Science

Drying is widely usedd and one of the most effective methods for food preservation. This study re... more Drying is widely usedd and one of the most effective methods for food preservation. This study reports the macro-mineral, crude protein, and antioxidant activity of locally produced powdered ginger and turmeric using the air-, oven- and sun drying methods. Kjeldahl method, atomic absorption spectroscopy, and phosphomolybdenum method were used to determine the crude protein, macrominerals (K, Ca, and Mg), and total antioxidant activity (TAA), respectively. Highest TAA was observed in sun-dried turmeric (7.82 ± 4.97mg AAE/g dried sample) while the lowest TAA was observed in sun-dried ginger powder (2.14 ± 0.31 mg AAE/mg dried sample). The measured crude protein of turmeric ranged from 8.34 ± 0.93 - 8.54 ± 0.14% while 6.25 ± 0.42 - 7.41 ± 1.05% for ginger. Essential macro-minerals in turmeric and ginger at varied drying methods ranged from 0.07 to 2.21 % for K, 0.04 to 0.12 % for% for Mg, and 0.79 to 1.15 % for Ca. ANOVA revealed that the effects of drying methods were only significant...

Research paper thumbnail of Antioxidant and Cytotoxic Activity of the Leaf Ethanolic Extracts of Tithonia diversofilia and Gliricidia sepium from Bukidnon, Philippines

Asian Journal of Biological and Life Sciences, 2019

Introduction: Nature has been an attractive source of new therapeutic candidate compounds. The pl... more Introduction: Nature has been an attractive source of new therapeutic candidate compounds. The plants produce naturally occurring secondary metabolites which are being investigated for their anticancer and antioxidant activities. Tithonia diversifolia and Gliricidia sepium are commonly growing in Bukidnon, Philippines. These plants have long list of ethno-medicinal uses. Thus, there is a need to further investigate their phytochemical constituents as well as their biological activities. Objectives: The study focuses on the phytochemicals in the ethanolic leaf extracts of T. diversifolia and G. sepium collected from Bukidnon, Philippines. Moreover, cytotoxic and antioxidant properties of the extracts were also determined through Brine Shrimp Lethality Assay (BSLA) and Total Antioxidant Capacity, respectively. Materials and Methods: Qualitative phytochemical screening and determination of Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Antioxidant Capacity (TAC) and cytotoxicity of the ethanolic leaf extracts of T. diversifolia and G. sepium were conducted. Results and Discussion: Results of the study showed the presence of flavonoids, phenolics, tannins and terpenoids in the ethanolic leaf extracts of T. diversifolia and G. sepium. The TPC of T. diversifolia (15.20 mg GAE/g dry sample) was found higher than that of G. sepium (14.43 mg GAE/g dry sample). Similar trend was also observed in the TFC where T. diversifolia and G. sepium recorded 12.50 and 9.00 mg QE/g dry sample, respectively. T. diversifolia also gave higher TAC of 302.8 mg AAE/ g dry sample than G. sepium (200.2 mg AAE/g dry sample). For cytotoxicity test, T. diversifolia (LC 50 = 14.57 mg/L) exhibited more potent toxicity as compared to G. sepium (LC 50 = 15.85 mg/L). The results indicate that the detected phytochemicals may account for the exhibited biological activities of T. diversifolia and G. sepium. Conclusion: This study uncovers the promising antioxidative and cytotoxic property of G. sepium and T. diversifolia that warrants further investigation.

Research paper thumbnail of Cytotoxicity, Antioxidant Activity, and Total Phenolic Content of the Ethanolic Leaf Extract of Hornstedtia conoidea Ridl

Asian Journal of Biological and Life sciences

Research paper thumbnail of The membrane-tethered NAC transcription factor, AtNTL7, contributes to ER-stress resistance in Arabidopsis

Biochemical and biophysical research communications, Jul 12, 2017

We screened for endoplasmic reticulum (ER) stress-resistant mutants among 25 mutants of the Arabi... more We screened for endoplasmic reticulum (ER) stress-resistant mutants among 25 mutants of the Arabidopsis NTL (NAC with Transmembrane motif 1-Like) family. We identified a novel mutant, SALK_044777, showing strong resistance to ER stress. RT-PCR and genomic DNA sequence analyses identified the mutant as atntl7, which harbors a T-DNA insertion in the fourth exon of AtNTL7. Two other atntl7-mutant alleles, in which T-DNA was inserted in the second exon and third intron of AtNTL7, respectively, showed ER-stress sensitive phenotypes, suggesting that SALK_044777 is a gain-of-function mutant. Arabidopsis plants overexpressing AtNTL7 showed strong ER-stress resistance. Our findings suggest that AtNTL7 fragment is cleaved from the ER membrane under ER stress and translocates into the nucleus to induce downstream ER-stress responsive genes.

Research paper thumbnail of Antibacterial activity of the crude ethanolic extracts of Etlingera elatior and Etlingera philippinensis

Antibacterial, Etlingera elatior, Etlingera philippinensis

Research paper thumbnail of Universal Stress Protein Exhibits a Redox-Dependent Chaperone Function in Arabidopsis and Enhances Plant Tolerance to Heat Shock and Oxidative Stress

Frontiers in Plant Science, 2015

Although a wide range of physiological information on Universal Stress Proteins (USPs) is availab... more Although a wide range of physiological information on Universal Stress Proteins (USPs) is available from many organisms, their biochemical, and molecular functions remain unidentified. The biochemical function of AtUSP (At3g53990) from Arabidopsis thaliana was therefore investigated. Plants over-expressing AtUSP showed a strong resistance to heat shock and oxidative stress, compared with wild-type and Atusp knockout plants, confirming the crucial role of AtUSP in stress tolerance. AtUSP was present in a variety of structures including monomers, dimers, trimers, and oligomeric complexes, and switched in response to external stresses from low molecular weight (LMW) species to high molecular weight (HMW) complexes. AtUSP exhibited a strong chaperone function under stress conditions in particular, and this activity was significantly increased by heat treatment. Chaperone activity of AtUSP was critically regulated by the redox status of cells and accompanied by structural changes to the protein. Over-expression of AtUSP conferred a strong tolerance to heat shock and oxidative stress upon Arabidopsis, primarily via its chaperone function.