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Research paper thumbnail of A new molecule reduces the cocoon production loss in the BmNPV infected Bombyx mori larvae rearing

A new molecule reduces the cocoon production loss in the BmNPV infected Bombyx mori larvae rearing

Bombyx mori nucleopolyhedrovirus, BmNPV, is the pathogen that causes mortality by the grasserie d... more Bombyx mori nucleopolyhedrovirus, BmNPV, is the pathogen that causes mortality by the grasserie disease in larval Bombyx mori (Lepidoptera:Bombycidae). A molecule named Bm5 was reported as an inhibitor candidate of viral cathepsin (v-cath), which may protect B. mori from this disease. This study evaluated the efficacy of Bm5 in reducing sericultural loss due to BmNPV. The assays were carried out using B. mori fifth-instar hybrids that were inoculated with BmNPV and treated with Bm5. We recorded biological and productive variables to verify the effectiveness of the Bm5 treatment. Data on biological and economic parameters showed that the larval survival rate of the BmNPV-infected and Bm5-treated groups was 35.6% higher than that of the untreated BmNPV group. In addition, the pupae from the Bm5 treatment group survived and produced good-quality cocoons. The results of esterase dosage from the midgut and fat body showed that no change was detected in isoenzyme expression in BmNPV-infec...

Research paper thumbnail of Identification and characterization of stem-bulge RNAs in Drosophila melanogaster

RNA Biology, Feb 2, 2019

Non-coding Y RNAs and stem-bulge RNAs are homologous small RNAs in vertebrates and nematodes, res... more Non-coding Y RNAs and stem-bulge RNAs are homologous small RNAs in vertebrates and nematodes, respectively. They share a conserved function in the replication of chromosomal DNA in these two groups of organisms. However, functional homologues have not been found in insects, despite their common early evolutionary history. Here, we describe the identification and functional characterization of two sbRNAs in Drosophila melanogaster, termed Dm1 and Dm2. The genes coding for these two RNAs were identified by a computational search in the genome of D. melanogaster for conserved sequence motifs present in nematode sbRNAs. The predicted secondary structures of Dm1 and Dm2 partially resemble nematode sbRNAs and show stability in molecular dynamics simulations. Both RNAs are phylogenetically closer related to nematode sbRNAs than to vertebrate Y RNAs. Dm1, but not Dm2 sbRNA is abundantly expressed in D. melanogaster S2 cells and adult flies. Only Dm1, but not Dm2 sbRNA can functionally replace Y RNAs in a human cell-free DNA replication initiation system. Therefore, Dm1 is the first functional sbRNA described in insects, allowing future investigations into the physiological roles of sbRNAs in the genetically tractable model organism D. melanogaster.

Research paper thumbnail of Comparação da expressão relativa dos Dm sbRNAs em duas linhagens celulares de Drosophila melanogaster

Comparação da expressão relativa dos Dm sbRNAs em duas linhagens celulares de Drosophila melanogaster

UNIVERSIDADE CESUMAR, Oct 30, 2019

Research paper thumbnail of Comparação da expressão relativa dos Dm sbRNAs em duas linhagens celulares de Drosophila melanogaster

Comparação da expressão relativa dos Dm sbRNAs em duas linhagens celulares de Drosophila melanogaster

Research paper thumbnail of Hygiene-sanitary analysis of fried pork skins after the process points of fretting and salting, and determining contamination sources

Hygiene-sanitary analysis of fried pork skins after the process points of fretting and salting, and determining contamination sources

Research paper thumbnail of Identification and characterization of stem-bulge RNAs in Drosophila melanogaster

RNA Biology

Non-coding Y RNAs and stem-bulge RNAs are homologous small RNAs in vertebrates and nematodes, res... more Non-coding Y RNAs and stem-bulge RNAs are homologous small RNAs in vertebrates and nematodes, respectively. They share a conserved function in the replication of chromosomal DNA in these two groups of organisms. However, functional homologues have not been found in insects, despite their common early evolutionary history. Here, we describe the identification and functional characterization of two sbRNAs in Drosophila melanogaster, termed Dm1 and Dm2. The genes coding for these two RNAs were identified by a computational search in the genome of D. melanogaster for conserved sequence motifs present in nematode sbRNAs. The predicted secondary structures of Dm1 and Dm2 partially resemble nematode sbRNAs and show stability in molecular dynamics simulations. Both RNAs are phylogenetically closer related to nematode sbRNAs than to vertebrate Y RNAs. Dm1, but not Dm2 sbRNA is abundantly expressed in D. melanogaster S2 cells and adult flies. Only Dm1, but not Dm2 sbRNA can functionally replace Y RNAs in a human cell-free DNA replication initiation system. Therefore, Dm1 is the first functional sbRNA described in insects, allowing future investigations into the physiological roles of sbRNAs in the genetically tractable model organism D. melanogaster.

Research paper thumbnail of A new molecule reduces the cocoon production loss in the BmNPV infected Bombyx mori larvae rearing

A new molecule reduces the cocoon production loss in the BmNPV infected Bombyx mori larvae rearing

Bombyx mori nucleopolyhedrovirus, BmNPV, is the pathogen that causes mortality by the grasserie d... more Bombyx mori nucleopolyhedrovirus, BmNPV, is the pathogen that causes mortality by the grasserie disease in larval Bombyx mori (Lepidoptera:Bombycidae). A molecule named Bm5 was reported as an inhibitor candidate of viral cathepsin (v-cath), which may protect B. mori from this disease. This study evaluated the efficacy of Bm5 in reducing sericultural loss due to BmNPV. The assays were carried out using B. mori fifth-instar hybrids that were inoculated with BmNPV and treated with Bm5. We recorded biological and productive variables to verify the effectiveness of the Bm5 treatment. Data on biological and economic parameters showed that the larval survival rate of the BmNPV-infected and Bm5-treated groups was 35.6% higher than that of the untreated BmNPV group. In addition, the pupae from the Bm5 treatment group survived and produced good-quality cocoons. The results of esterase dosage from the midgut and fat body showed that no change was detected in isoenzyme expression in BmNPV-infec...

Research paper thumbnail of Identification and characterization of stem-bulge RNAs in Drosophila melanogaster

RNA Biology, Feb 2, 2019

Non-coding Y RNAs and stem-bulge RNAs are homologous small RNAs in vertebrates and nematodes, res... more Non-coding Y RNAs and stem-bulge RNAs are homologous small RNAs in vertebrates and nematodes, respectively. They share a conserved function in the replication of chromosomal DNA in these two groups of organisms. However, functional homologues have not been found in insects, despite their common early evolutionary history. Here, we describe the identification and functional characterization of two sbRNAs in Drosophila melanogaster, termed Dm1 and Dm2. The genes coding for these two RNAs were identified by a computational search in the genome of D. melanogaster for conserved sequence motifs present in nematode sbRNAs. The predicted secondary structures of Dm1 and Dm2 partially resemble nematode sbRNAs and show stability in molecular dynamics simulations. Both RNAs are phylogenetically closer related to nematode sbRNAs than to vertebrate Y RNAs. Dm1, but not Dm2 sbRNA is abundantly expressed in D. melanogaster S2 cells and adult flies. Only Dm1, but not Dm2 sbRNA can functionally replace Y RNAs in a human cell-free DNA replication initiation system. Therefore, Dm1 is the first functional sbRNA described in insects, allowing future investigations into the physiological roles of sbRNAs in the genetically tractable model organism D. melanogaster.

Research paper thumbnail of Comparação da expressão relativa dos Dm sbRNAs em duas linhagens celulares de Drosophila melanogaster

Comparação da expressão relativa dos Dm sbRNAs em duas linhagens celulares de Drosophila melanogaster

UNIVERSIDADE CESUMAR, Oct 30, 2019

Research paper thumbnail of Comparação da expressão relativa dos Dm sbRNAs em duas linhagens celulares de Drosophila melanogaster

Comparação da expressão relativa dos Dm sbRNAs em duas linhagens celulares de Drosophila melanogaster

Research paper thumbnail of Hygiene-sanitary analysis of fried pork skins after the process points of fretting and salting, and determining contamination sources

Hygiene-sanitary analysis of fried pork skins after the process points of fretting and salting, and determining contamination sources

Research paper thumbnail of Identification and characterization of stem-bulge RNAs in Drosophila melanogaster

RNA Biology

Non-coding Y RNAs and stem-bulge RNAs are homologous small RNAs in vertebrates and nematodes, res... more Non-coding Y RNAs and stem-bulge RNAs are homologous small RNAs in vertebrates and nematodes, respectively. They share a conserved function in the replication of chromosomal DNA in these two groups of organisms. However, functional homologues have not been found in insects, despite their common early evolutionary history. Here, we describe the identification and functional characterization of two sbRNAs in Drosophila melanogaster, termed Dm1 and Dm2. The genes coding for these two RNAs were identified by a computational search in the genome of D. melanogaster for conserved sequence motifs present in nematode sbRNAs. The predicted secondary structures of Dm1 and Dm2 partially resemble nematode sbRNAs and show stability in molecular dynamics simulations. Both RNAs are phylogenetically closer related to nematode sbRNAs than to vertebrate Y RNAs. Dm1, but not Dm2 sbRNA is abundantly expressed in D. melanogaster S2 cells and adult flies. Only Dm1, but not Dm2 sbRNA can functionally replace Y RNAs in a human cell-free DNA replication initiation system. Therefore, Dm1 is the first functional sbRNA described in insects, allowing future investigations into the physiological roles of sbRNAs in the genetically tractable model organism D. melanogaster.