Camilo Duque - Academia.edu (original) (raw)
Papers by Camilo Duque
Revista Politécnica, 2020
El conocimiento del Modelo Hidrogeológico Conceptual permite adoptar y adaptar un procedimiento a... more El conocimiento del Modelo Hidrogeológico Conceptual permite adoptar y adaptar un procedimiento adecuado para cada caso de estudio, considerando acuíferos libres y acuíferos confinados, teniendo en cuenta las diferencias estructurales entre rocas duras, rocas blandas y depósitos no consolidados; y evaluando el rol de la textura y la capacidad de infiltración del suelo, en la dimensión de la función de la recarga directa. Para el sistema hidrogeológico del Eje Bananero del Urabá Antioqueño- Colombia la tarea de entender la recarga puso de manifiesto la necesidad de establecer una escala de calificación para evaluar el factor estructural en rocas blandas y depósitos no consolidados y definir categorías de importancia, basados en propiedades texturales y tasas de infiltración para las zonas de recarga directa difusa en acuíferos libres; también se determinó un procedimiento para delimitar zonas asociadas a las unidades hidrogeológicas que subrayasen a los acuíferos confinados.
BackgroundThe Zika AIRS Project, a USAID-funded initiative worked across the Latin America and Ca... more BackgroundThe Zika AIRS Project, a USAID-funded initiative worked across the Latin America and Caribbean regions from 2016 to 2019, as an emergency to contain the spread of the Zika virus. All entomological records in the target countries showed wide distribution and high abundance of Aedes aegypti populations, however the susceptibility profiles of these insects to insecticides commonly employed by vector control campaigns were in most cases incomplete or inexistent. In close collaboration with the Ministries of Health of individual countries, Zika-AIRS teams conducted insecticide susceptibility testing of an array of insecticides in A. aegypti populations of each country. Procedures applied met the standard international protocols instructed by the World Health Organization and Centers for Disease Control and Prevention.Methodology and main findingsThe insecticides tested were selected under categories such as pyrethroids, organophosphates and carbamate. Results showed A. aegypti ...
Revista Colombiana De Cirugia, Jun 1, 2013
![Research paper thumbnail of [Symptoms of myasthenia gravis in a patient with a history of thymectomy for invasive thymoma]](https://attachments.academia-assets.com/94990491/thumbnails/1.jpg)
](Biomédica : revista del Instituto Nacional de Salud, 2015
Myasthenia gravis is an antibody-mediated autoimmune disease. Approximately 10-15% of patients pr... more Myasthenia gravis is an antibody-mediated autoimmune disease. Approximately 10-15% of patients present with a thymoma, the presence of which is associated with greater severity of symptoms, myasthenic crisis, and irresponsiveness to front-line therapy. A thymectomy is recommended in young patients with generalized myasthenia gravis and in all patients presenting with thymoma. The patient was a 43-year-old woman, who first showed symptoms of myasthenic crisis in 2005 and presented with invasive thymoma managed with thymectomy and radiotherapy. In the subsequent three years, the patient presented with severe symptoms and two myasthenic crises that required mechanical ventilation and immunoglobulin treatment. Contrast chest computed tomography examinations showed no recurrence. Between 2009 and 2012, the patient experienced decreased symptom severity. In 2013, the patient presented with an exacerbation of symptoms; a contrast chest magnetic resonance scan showed a lesion in the anterio...
Revista Salud Bosque, 2015
A partir de la reflexión sobre la utilización despectiva de la retórica en la ciencia, en este en... more A partir de la reflexión sobre la utilización despectiva de la retórica en la ciencia, en este ensayo se reseñan las principales formas de representación de los supuestos alcances de los fluoruros como medidas de salud pública. De manera crítica, se concluye que los discursos científicos, no exentos de la retórica, atribuyen a los fluoruros efectos que no tienen dentro de la misma lógica en que fueron construidos. Es, pues, una invitación a la recuperación de la retórica para el análisis de los problemas que aborda la ciencia, y de las políticas públicas que se han montado sobre diferentes discursos que representan estos problemas desde perspectivas evidentemente interesadas
En septiembre de 2009, la Comisión de la Universidad Nacional para la celebración del Bicentenari... more En septiembre de 2009, la Comisión de la Universidad Nacional para la celebración del Bicentenario de la Independencia publicó la edición facsimilar de la obra Planches anatomiques du corps humain: exécutées d´aprés les dimensions naturelles, acompagnees d´un texte explicatif par F. Antommarchi, 1926.En Anatomía y arte: a propósito de este atlas anatómico se presentan ensayos en los que se situan teórica y temporalmente el atlas anatómico.
American Journal of Physiology-Renal Physiology, 2014
Stenosis of arteriovenous (A-V) fistulae secondary to neointimal hyperplasia (NIH) compromises di... more Stenosis of arteriovenous (A-V) fistulae secondary to neointimal hyperplasia (NIH) compromises dialysis delivery, which worsens patients' quality of life and increases medical costs associated with the maintenance of vascular accesses. In the present study, we evaluated the role of the receptor tyrosine kinase c-Kit in A-V fistula neointima formation. Initially, c-Kit was found in the neointima and adventitia of human brachiobasilic fistulae, whereas it was barely detectable in control veins harvested at the time of access creation. Using the rat A-V fistula model to study venous vascular remodeling, we analyzed the spatial and temporal pattern of c-Kit expression in the fistula wall. Interestingly, c-Kit immunoreactivity increased with time after anastomosis, which concurred with the accumulation of cells in the venous intima. In addition, c-Kit expression in A-V fistulae was positively altered by chronic kidney failure conditions. Both blockade of c-Kit with imatinib mesylate ...
Surgical Laparoscopy, Endoscopy & Percutaneous Techniques, 2013
Introduction: Germ line mutations in the E-cadherin gene account for approximately 30% to 50% of ... more Introduction: Germ line mutations in the E-cadherin gene account for approximately 30% to 50% of cases of hereditary diffuse gastric cancer syndrome. The estimated cumulative risk of gastric cancer by the age of 80 years in hereditary diffuse gastric cancer families is 67% for men and 83% for women, both of which carry a very poor prognosis if an early diagnosis is not made. Materials and Methods: Two siblings with a strong family history of gastric cancer over at least 2 generations, including an aunt testing positive for the E-cadherin gene mutation, presented with positive results of the same mutation. Both siblings underwent a laparoscopic total gastrectomy. The postoperative period was uneventful. A 7-trocar totally laparoscopic surgical approach was used. The esophagojejunal anastomosis was created using a linear stapler in a lateral side-to-side manner. The biliopancreatic limb was 50 cm and the Roux limb was 75 cm in length. Results: Both siblings recovered without complications. Histologic evaluation revealed benign gastric tissue with no evidence of malignancy. Margins were clear of gastric mucosa. Prophylactic total gastrectomy eliminates the risk of gastric cancer in individuals with E-cadherin gene mutations. This procedure can be performed safely with a laparoscopic approach, resulting in excellent recovery and prognosis. Totally laparoscopic, lateral side-to-side esophagojejunal anastomosis using linear stapler creates an emerged lumen of both the esophagus and the jejunum that carries less risk of stricture, and reflux is prevented by a 75-cm-long Roux limb.
Endocrinology Studies, 2013
Endocrinology Studies 2013; volume 3:e7
Seminars in Dialysis, 2014
Cardiac hypertrophy is a relatively common complication seen in patients with advanced chronic ki... more Cardiac hypertrophy is a relatively common complication seen in patients with advanced chronic kidney disease (CKD) and end-stage renal disease (ESRD). Moreover, cardiac hypertrophy is even more frequently seen in patients with ESRD who have an arteriovenous (AV) access. There has been substantial evidence pertaining to the effects of AV access creation on the heart structure and function. Similarly, there is increasing evidence on the effects of AV access closure, flow reduction, transplantation, and immunosuppressive medication on both endpoints. In this review, we present the evidence available in the literature on these topics and open the dialog for further research in this interesting field.
Kidney International, 2014
Lipopolysaccharide pretreatment protects from renal ischemia/reperfusion injury: possible connect... more Lipopolysaccharide pretreatment protects from renal ischemia/reperfusion injury: possible connection to an interleukin-6dependent pathway.
Journal of Biomedical Materials Research, 2000
The physical and chemical properties of biochar vary based on feedstock sources and production co... more The physical and chemical properties of biochar vary based on feedstock sources and production conditions, making it possible to engineer biochars with specific functions (e.g. carbon sequestration, soil quality improvements, or contaminant sorption). In 2013, the International Biochar Initiative (IBI) made publically available their Standardized Product Definition and Product Testing Guidelines (Version 1.1) which set standards for physical and chemical characteristics for biochar. Six biochars made from three different feedstocks and at two temperatures were analyzed for characteristics related to their use as a soil amendment. The protocol describes analyses of the feedstocks and biochars and includes: cation exchange capacity (CEC), specific surface area (SSA), organic carbon (OC) and moisture percentage, pH, particle size distribution, and proximate and ultimate analysis. Also described in the protocol are the analyses of the feedstocks and biochars for contaminants including polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), metals and mercury as well as nutrients (phosphorous, nitrite and nitrate and ammonium as nitrogen). The protocol also includes the biological testing procedures, earthworm avoidance and germination assays. Based on the quality assurance / quality control (QA/QC) results of blanks, duplicates, standards and reference materials, all methods were determined adequate for use with biochar and feedstock materials. All biochars and feedstocks were well within the criterion set by the IBI and there were little differences among biochars, except in the case of the biochar produced from construction waste materials. This biochar (referred to as Old biochar) was determined to have elevated levels of arsenic, chromium, copper, and lead, and failed the earthworm avoidance and germination assays. Based on these results, Old biochar would not be appropriate for use as a soil amendment for carbon sequestration, substrate quality improvements or remediation.
FEMS Microbiology Letters, 2012
Several genomes of different Mycobacterium tuberculosis isolates have been completely sequenced a... more Several genomes of different Mycobacterium tuberculosis isolates have been completely sequenced around the world. The genomic information obtained have shown higher diversity than originally thought and specific adaptations to different human populations. Within this work, we sequenced the genome of one Colombian M. tuberculosis virulent isolate. Genomic comparison against the reference genome of H37Rv and other strains showed multiple deletion and insertions that ranged between a few bases to thousands. Excluding PPE and PG-PGRS genes, 430 proteins present changes in at least 1 amino acid. Also, novel positions of the IS6110 mobile element were identified. This isolate is also characterized by a large genomic deletion of 3.6 kb, leading to the loss and modification of the dosR regulon genes, Rv1996 and Rv1997. To our knowledge, this is the first report of the genome sequence of a Latin American M. tuberculosis clinical isolate.
Revista CES Medicina, 2009
Coronary artery disease is today the number one cause of mortality worldwide. For doctors it is o... more Coronary artery disease is today the number one cause of mortality worldwide. For doctors it is of the utmost importance to know the best possible way to approach these patients. The problem is that there is a lot of information on the subject and it is not always clear or easily applicable. This review intends to go over and explain the tools needed-adapted to the Colombian reality, in order for the clinician to be able and engage these patients knowing "the why" of the actions taken. The four topics where most of the mistakes are made will be reviewed, these are: evaluation of the probability, risk stratification, electrocardiogram interpretation and correct use of biomarkers. KEY WORDS Chest pain Chest discomfort Coronary artery disease Risk Biomarkers Diagnosis ¿Por qué hablar del evento coronario agudo (ECA)? Es la causa número uno de mortalidad en el mundo, en Colombia y en Antioquia (1-2). Es una causa muy importante de error médico y de la cual se deriva gran morbilidad; en EEUU
Biochimica et Biophysica Acta (BBA) - General Subjects, 2013
BACKGROUND-CD11b/CD18 is a key adhesion receptor that mediates leukocyte adhesion, migration and ... more BACKGROUND-CD11b/CD18 is a key adhesion receptor that mediates leukocyte adhesion, migration and immune functions. We recently identified novel compounds, leukadherins, that allosterically enhance CD11b/CD18-dependent cell adhesion and reduce inflammation in vivo, suggesting integrin activation to be a novel mechanism of action for the development of antiinflammatory therapeutics. Since a number of well-characterized anti-CD11b/CD18 activating antibodies are currently available, we wondered if such biological agonists could also become therapeutic leads following this mechanism of action. METHODS-We compared the two types of agonists using in vitro cell adhesion and woundhealing assays and using animal model systems. We also studied effects of the two types of agonists on outside-in signaling in treated cells. RESULTS-Both types of agonists similarly enhanced integrin-mediated cell adhesion and decreased cell migration. However, unlike leukadherins, the activating antibodies produced significant CD11b/CD18 macro clustering and induced phosphorylation of key proteins involved in outside-in signaling. Studies using conformation reporter antibodies showed that leukadherins did not induce global conformational changes in CD11b/CD18 explaining the reason behind their lack of ligand-mimetic outside-in signaling. In vivo, leukadherins reduced vascular injury in a
Revista Politécnica, 2020
El conocimiento del Modelo Hidrogeológico Conceptual permite adoptar y adaptar un procedimiento a... more El conocimiento del Modelo Hidrogeológico Conceptual permite adoptar y adaptar un procedimiento adecuado para cada caso de estudio, considerando acuíferos libres y acuíferos confinados, teniendo en cuenta las diferencias estructurales entre rocas duras, rocas blandas y depósitos no consolidados; y evaluando el rol de la textura y la capacidad de infiltración del suelo, en la dimensión de la función de la recarga directa. Para el sistema hidrogeológico del Eje Bananero del Urabá Antioqueño- Colombia la tarea de entender la recarga puso de manifiesto la necesidad de establecer una escala de calificación para evaluar el factor estructural en rocas blandas y depósitos no consolidados y definir categorías de importancia, basados en propiedades texturales y tasas de infiltración para las zonas de recarga directa difusa en acuíferos libres; también se determinó un procedimiento para delimitar zonas asociadas a las unidades hidrogeológicas que subrayasen a los acuíferos confinados.
BackgroundThe Zika AIRS Project, a USAID-funded initiative worked across the Latin America and Ca... more BackgroundThe Zika AIRS Project, a USAID-funded initiative worked across the Latin America and Caribbean regions from 2016 to 2019, as an emergency to contain the spread of the Zika virus. All entomological records in the target countries showed wide distribution and high abundance of Aedes aegypti populations, however the susceptibility profiles of these insects to insecticides commonly employed by vector control campaigns were in most cases incomplete or inexistent. In close collaboration with the Ministries of Health of individual countries, Zika-AIRS teams conducted insecticide susceptibility testing of an array of insecticides in A. aegypti populations of each country. Procedures applied met the standard international protocols instructed by the World Health Organization and Centers for Disease Control and Prevention.Methodology and main findingsThe insecticides tested were selected under categories such as pyrethroids, organophosphates and carbamate. Results showed A. aegypti ...
Revista Colombiana De Cirugia, Jun 1, 2013
Biomédica : revista del Instituto Nacional de Salud, 2015
Myasthenia gravis is an antibody-mediated autoimmune disease. Approximately 10-15% of patients pr... more Myasthenia gravis is an antibody-mediated autoimmune disease. Approximately 10-15% of patients present with a thymoma, the presence of which is associated with greater severity of symptoms, myasthenic crisis, and irresponsiveness to front-line therapy. A thymectomy is recommended in young patients with generalized myasthenia gravis and in all patients presenting with thymoma. The patient was a 43-year-old woman, who first showed symptoms of myasthenic crisis in 2005 and presented with invasive thymoma managed with thymectomy and radiotherapy. In the subsequent three years, the patient presented with severe symptoms and two myasthenic crises that required mechanical ventilation and immunoglobulin treatment. Contrast chest computed tomography examinations showed no recurrence. Between 2009 and 2012, the patient experienced decreased symptom severity. In 2013, the patient presented with an exacerbation of symptoms; a contrast chest magnetic resonance scan showed a lesion in the anterio...
Revista Salud Bosque, 2015
A partir de la reflexión sobre la utilización despectiva de la retórica en la ciencia, en este en... more A partir de la reflexión sobre la utilización despectiva de la retórica en la ciencia, en este ensayo se reseñan las principales formas de representación de los supuestos alcances de los fluoruros como medidas de salud pública. De manera crítica, se concluye que los discursos científicos, no exentos de la retórica, atribuyen a los fluoruros efectos que no tienen dentro de la misma lógica en que fueron construidos. Es, pues, una invitación a la recuperación de la retórica para el análisis de los problemas que aborda la ciencia, y de las políticas públicas que se han montado sobre diferentes discursos que representan estos problemas desde perspectivas evidentemente interesadas
En septiembre de 2009, la Comisión de la Universidad Nacional para la celebración del Bicentenari... more En septiembre de 2009, la Comisión de la Universidad Nacional para la celebración del Bicentenario de la Independencia publicó la edición facsimilar de la obra Planches anatomiques du corps humain: exécutées d´aprés les dimensions naturelles, acompagnees d´un texte explicatif par F. Antommarchi, 1926.En Anatomía y arte: a propósito de este atlas anatómico se presentan ensayos en los que se situan teórica y temporalmente el atlas anatómico.
American Journal of Physiology-Renal Physiology, 2014
Stenosis of arteriovenous (A-V) fistulae secondary to neointimal hyperplasia (NIH) compromises di... more Stenosis of arteriovenous (A-V) fistulae secondary to neointimal hyperplasia (NIH) compromises dialysis delivery, which worsens patients' quality of life and increases medical costs associated with the maintenance of vascular accesses. In the present study, we evaluated the role of the receptor tyrosine kinase c-Kit in A-V fistula neointima formation. Initially, c-Kit was found in the neointima and adventitia of human brachiobasilic fistulae, whereas it was barely detectable in control veins harvested at the time of access creation. Using the rat A-V fistula model to study venous vascular remodeling, we analyzed the spatial and temporal pattern of c-Kit expression in the fistula wall. Interestingly, c-Kit immunoreactivity increased with time after anastomosis, which concurred with the accumulation of cells in the venous intima. In addition, c-Kit expression in A-V fistulae was positively altered by chronic kidney failure conditions. Both blockade of c-Kit with imatinib mesylate ...
Surgical Laparoscopy, Endoscopy & Percutaneous Techniques, 2013
Introduction: Germ line mutations in the E-cadherin gene account for approximately 30% to 50% of ... more Introduction: Germ line mutations in the E-cadherin gene account for approximately 30% to 50% of cases of hereditary diffuse gastric cancer syndrome. The estimated cumulative risk of gastric cancer by the age of 80 years in hereditary diffuse gastric cancer families is 67% for men and 83% for women, both of which carry a very poor prognosis if an early diagnosis is not made. Materials and Methods: Two siblings with a strong family history of gastric cancer over at least 2 generations, including an aunt testing positive for the E-cadherin gene mutation, presented with positive results of the same mutation. Both siblings underwent a laparoscopic total gastrectomy. The postoperative period was uneventful. A 7-trocar totally laparoscopic surgical approach was used. The esophagojejunal anastomosis was created using a linear stapler in a lateral side-to-side manner. The biliopancreatic limb was 50 cm and the Roux limb was 75 cm in length. Results: Both siblings recovered without complications. Histologic evaluation revealed benign gastric tissue with no evidence of malignancy. Margins were clear of gastric mucosa. Prophylactic total gastrectomy eliminates the risk of gastric cancer in individuals with E-cadherin gene mutations. This procedure can be performed safely with a laparoscopic approach, resulting in excellent recovery and prognosis. Totally laparoscopic, lateral side-to-side esophagojejunal anastomosis using linear stapler creates an emerged lumen of both the esophagus and the jejunum that carries less risk of stricture, and reflux is prevented by a 75-cm-long Roux limb.
Endocrinology Studies, 2013
Endocrinology Studies 2013; volume 3:e7
Seminars in Dialysis, 2014
Cardiac hypertrophy is a relatively common complication seen in patients with advanced chronic ki... more Cardiac hypertrophy is a relatively common complication seen in patients with advanced chronic kidney disease (CKD) and end-stage renal disease (ESRD). Moreover, cardiac hypertrophy is even more frequently seen in patients with ESRD who have an arteriovenous (AV) access. There has been substantial evidence pertaining to the effects of AV access creation on the heart structure and function. Similarly, there is increasing evidence on the effects of AV access closure, flow reduction, transplantation, and immunosuppressive medication on both endpoints. In this review, we present the evidence available in the literature on these topics and open the dialog for further research in this interesting field.
Kidney International, 2014
Lipopolysaccharide pretreatment protects from renal ischemia/reperfusion injury: possible connect... more Lipopolysaccharide pretreatment protects from renal ischemia/reperfusion injury: possible connection to an interleukin-6dependent pathway.
Journal of Biomedical Materials Research, 2000
The physical and chemical properties of biochar vary based on feedstock sources and production co... more The physical and chemical properties of biochar vary based on feedstock sources and production conditions, making it possible to engineer biochars with specific functions (e.g. carbon sequestration, soil quality improvements, or contaminant sorption). In 2013, the International Biochar Initiative (IBI) made publically available their Standardized Product Definition and Product Testing Guidelines (Version 1.1) which set standards for physical and chemical characteristics for biochar. Six biochars made from three different feedstocks and at two temperatures were analyzed for characteristics related to their use as a soil amendment. The protocol describes analyses of the feedstocks and biochars and includes: cation exchange capacity (CEC), specific surface area (SSA), organic carbon (OC) and moisture percentage, pH, particle size distribution, and proximate and ultimate analysis. Also described in the protocol are the analyses of the feedstocks and biochars for contaminants including polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), metals and mercury as well as nutrients (phosphorous, nitrite and nitrate and ammonium as nitrogen). The protocol also includes the biological testing procedures, earthworm avoidance and germination assays. Based on the quality assurance / quality control (QA/QC) results of blanks, duplicates, standards and reference materials, all methods were determined adequate for use with biochar and feedstock materials. All biochars and feedstocks were well within the criterion set by the IBI and there were little differences among biochars, except in the case of the biochar produced from construction waste materials. This biochar (referred to as Old biochar) was determined to have elevated levels of arsenic, chromium, copper, and lead, and failed the earthworm avoidance and germination assays. Based on these results, Old biochar would not be appropriate for use as a soil amendment for carbon sequestration, substrate quality improvements or remediation.
FEMS Microbiology Letters, 2012
Several genomes of different Mycobacterium tuberculosis isolates have been completely sequenced a... more Several genomes of different Mycobacterium tuberculosis isolates have been completely sequenced around the world. The genomic information obtained have shown higher diversity than originally thought and specific adaptations to different human populations. Within this work, we sequenced the genome of one Colombian M. tuberculosis virulent isolate. Genomic comparison against the reference genome of H37Rv and other strains showed multiple deletion and insertions that ranged between a few bases to thousands. Excluding PPE and PG-PGRS genes, 430 proteins present changes in at least 1 amino acid. Also, novel positions of the IS6110 mobile element were identified. This isolate is also characterized by a large genomic deletion of 3.6 kb, leading to the loss and modification of the dosR regulon genes, Rv1996 and Rv1997. To our knowledge, this is the first report of the genome sequence of a Latin American M. tuberculosis clinical isolate.
Revista CES Medicina, 2009
Coronary artery disease is today the number one cause of mortality worldwide. For doctors it is o... more Coronary artery disease is today the number one cause of mortality worldwide. For doctors it is of the utmost importance to know the best possible way to approach these patients. The problem is that there is a lot of information on the subject and it is not always clear or easily applicable. This review intends to go over and explain the tools needed-adapted to the Colombian reality, in order for the clinician to be able and engage these patients knowing "the why" of the actions taken. The four topics where most of the mistakes are made will be reviewed, these are: evaluation of the probability, risk stratification, electrocardiogram interpretation and correct use of biomarkers. KEY WORDS Chest pain Chest discomfort Coronary artery disease Risk Biomarkers Diagnosis ¿Por qué hablar del evento coronario agudo (ECA)? Es la causa número uno de mortalidad en el mundo, en Colombia y en Antioquia (1-2). Es una causa muy importante de error médico y de la cual se deriva gran morbilidad; en EEUU
Biochimica et Biophysica Acta (BBA) - General Subjects, 2013
BACKGROUND-CD11b/CD18 is a key adhesion receptor that mediates leukocyte adhesion, migration and ... more BACKGROUND-CD11b/CD18 is a key adhesion receptor that mediates leukocyte adhesion, migration and immune functions. We recently identified novel compounds, leukadherins, that allosterically enhance CD11b/CD18-dependent cell adhesion and reduce inflammation in vivo, suggesting integrin activation to be a novel mechanism of action for the development of antiinflammatory therapeutics. Since a number of well-characterized anti-CD11b/CD18 activating antibodies are currently available, we wondered if such biological agonists could also become therapeutic leads following this mechanism of action. METHODS-We compared the two types of agonists using in vitro cell adhesion and woundhealing assays and using animal model systems. We also studied effects of the two types of agonists on outside-in signaling in treated cells. RESULTS-Both types of agonists similarly enhanced integrin-mediated cell adhesion and decreased cell migration. However, unlike leukadherins, the activating antibodies produced significant CD11b/CD18 macro clustering and induced phosphorylation of key proteins involved in outside-in signaling. Studies using conformation reporter antibodies showed that leukadherins did not induce global conformational changes in CD11b/CD18 explaining the reason behind their lack of ligand-mimetic outside-in signaling. In vivo, leukadherins reduced vascular injury in a