Denise Candido - Academia.edu (original) (raw)

Papers by Denise Candido

Toxicon, 2019

4388061115). Rutin succinate was synthetized and analyzed by C18 reversed-phase HPLC. Swiss mice ... more 4388061115). Rutin succinate was synthetized and analyzed by C18 reversed-phase HPLC. Swiss mice were injected with saline (negative control), BjV (1.6 mg/kg b.w., s.c., positive control) or BjV pre-incubated with rutin or rutin succinate. Rutin and rutin succinate were also injected in mice (14.4 mg/kg b.w. i.p.) 1 h after BjV administration. After 6 h of envenomation, samples were collected and hemorrhage was evaluated. Results were considered significant when p< 0.05. Results and Discussion: All envenomed mice showed a decrease in platelet counts and antioxidant capacity. Rutin prevented the venom-induced consumptive coagulopathy (decrease of fibrinogen levels). The use of rutin and rutin succinate decreased the hemorrhage in envenomated mice and surprisingly, rutin succinate pre-incubated with venom abolished skin hemorrhage. The results indicated a potential of the plant-based compounds rutin and rutin succinate as therapeutic agents. Currently, we are investigating the mechanisms of action of the venom, rutin, rutin succinate and its interactions. Such study provides tools to better understand the pathophysiology of hemostatic and hemorrhagic disturbances evoked by envenomation and may improve therapeutic strategies. Supported by São Paulo Research Foundation (FAPESP), CAPES, CNPq and Fundação Butantan.

Toxicon, Dec 1, 2018

Studies of scorpion venoms have used different venom drying methods: lyophilization, desiccation,... more Studies of scorpion venoms have used different venom drying methods: lyophilization, desiccation, lyophilization after mixing with 0.9% saline or purified water and centrifugation. The aim of this study was to see if these different approaches cause some alteration in the composition of the venom or interfere with its biological effects. Mice were injected (i.p.) with T. serrulatus scorpion venom in the liquid form (G-liq) or dried by different methods (lyophilized - G-lyo; centrifuged and the supernatant lyophilized - G-cen; desiccated - G-des), and observed regarding the occurrence of the symptoms respiratory difficulty, convulsion and death. The occurrence of seizures, although occurring in all groups and with the various doses used, did not prove to be effective to determine differences between the different handling techniques. Respiratory distress appeared to be useful in analyzing differences between groups, where this effect was less pronounced in the G-liq and G-des groups. In general, death occurred in a certain proportion with increasing dose for all groups. G-liq and G-des seemed to be more "active" at lower doses and G-cen and G-lyo at higher doses. The electrophoretic and chromatographic profile demonstrated main differences between G-liq and the dried groups. In the electrophoretic profile, the liquid venom showed bands of proteins of higher concentration and greater number of major bands and the three dried venom had the lowest number of protein bands. The HPLC profile and densitometry of the electrophoretic profiles showed some differences that may be associated with different protein conformation/aggregation. Our data indicated that lyophilization is the most suitable method for processing T. serrulatus scorpion venom after extraction.

Zenodo (CERN European Organization for Nuclear Research), Dec 31, 2006

De, Claudio Augusto R., Souza, Candido, Denise M., Lourenço, Wilson R. (2006): Description of the... more De, Claudio Augusto R., Souza, Candido, Denise M., Lourenço, Wilson R. (2006): Description of the male of Tityus martinpaechi Lourenço, 2001 (Scorpiones, Buthidae). Zootaxa 1260: 27-35, DOI: 10.5281/zenodo.173165

Zenodo (CERN European Organization for Nuclear Research), Dec 31, 2009

The species included in the Tityus stigmurus complex as established by Lourenço are revised. The ... more The species included in the Tityus stigmurus complex as established by Lourenço are revised. The male of Tityus serrulatus Lutz & Mello, is redescribed. New diagnostic characters are given for T.

PLOS Neglected Tropical Diseases, Jun 20, 2023

Background Scorpion stings in Brazil represent a major public health problem due to their inciden... more Background Scorpion stings in Brazil represent a major public health problem due to their incidence and their potential ability to lead to severe and often fatal clinical outcomes. A better understanding of scorpionism determinants is essential for a precise comprehension of accident dynamics and to guide public policy. Our study is the first to model the spatio-temporal variability of scorpionism across municipalities in São Paulo (SP) and to investigate its relationship with demographic, socioeconomic, environmental, and climatic variables. Methodology This ecological study analyzed secondary data on scorpion envenomation in SP from 2008 to 2021, using the Integrated Nested Laplace Approximation (INLA) to perform Bayesian inference for detection of areas and periods with the most suitable conditions for scorpionism. Principal findings From the spring of 2008 to 2021, the relative risk (RR) increased eight times in SP, from 0.47 (95%CI 0.43-0.51) to 3.57 (95%CI 3.36-3.78), although there has been an apparent stabilization since 2019. The western, northern, and northwestern parts of SP showed higher risks; overall, there was a 13% decrease in scorpionism during winters. Among the covariates considered, an increase of one standard deviation in the Gini index, which captures income inequality, was associated with a 11% increase in scorpion envenomation. Maximum temperatures were also associated with scorpionism, with risks doubling for temperatures above 36˚C. Relative humidity displayed a nonlinear association, with a 50% PLOS NEGLECTED TROPICAL DISEASES

Journal of Venomous Animals and Toxins including Tropical Diseases

Venom from Amazonian scorpions of the genus Tityus contains components capable of eliciting a dis... more Venom from Amazonian scorpions of the genus Tityus contains components capable of eliciting a distinct clinical, mostly neurological, syndrome. This contrasts with the mainly autonomic manifestations produced after envenomation by congeneric southern and northern South American species. Herein, we summarize Pan-Amazonian scorpionism by synthesizing available toxinological, clinical, and molecular data gathered from all affected areas in Amazonia, including Brazil, Ecuador, Colombia, Peru, Venezuela, and French Guiana. We searched multiple databases, as well as our own records, for reports of scorpion envenomations in Amazonia by confirmed Tityus spp., and compared the clinical manifestations. To help uncover clinical and venom relationships among problematic species, we explored phylogenetic relationships with a rate-calibrated analysis of mitochondrial COI data from available species. The possible existence of diversity gradients for venom toxic and immunogenic components despite the predicted strong phylogenetic association among species is underscored by discussed clinical and toxinological findings. A multicentric effort, involving all nations affected by this neglected disease, is urgently needed to offer alternatives for treating and understanding this pathology, including the preparation of neutralizing antibodies with a broad range of efficacy.

PLOS ONE

Scorpion envenomation is a significant public health concern in São Paulo, Brazil, and its incide... more Scorpion envenomation is a significant public health concern in São Paulo, Brazil, and its incidence and mortality have increased in recent decades. The present study analyzed documented scorpion envenomation notifications from 2008 to 2018 throughout the 645 municipalities of São Paulo. Annual incidence and mortality rates were calculated and stratified according to sex and age. The local empirical Bayesian method and Getis-Ord Gi* statistic were used to represent standardized incidence rates in the municipalities and to identify high- and low-risk agglomerates. The incidence rate of scorpion envenomation quintupled between 2008 and 2018. Overall, the risk was higher for man, and increased with age. Deaths due to envenomation, however, were concentrated almost entirely in children 0–9 years of age. Incidence maps showed that the risk of envenomation increased in almost all regions and municipalities of São Paulo throughout the study period. The highest incidence rates were found in...

Acta Tropica, 2022

Accidents caused by scorpions are considered a neglected condition and represent a major health p... more Accidents caused by scorpions are considered a neglected condition and represent a major health problem in most tropical countries, especially for children and elderly people. In Brazil, scorpionism is recurrent in the southeast region, mainly in the state of São Paulo, due to the progressive increase in scorpions found in urban habitats. Thus, our study aimed to provide better insights into the geographic and epidemiological characteristics of scorpion envenomation in São Paulo state and identify the environmental factors that are associated with these accidents. This is an ecological and retrospective study with secondary data on scorpion accidents in the state of São Paulo from 2008 to 2018 obtained from the Notifiable Disease Information System. The SatScan software was used to identify the higher- and lower-risk spatiotemporal clusters. A total of 145,464 scorpion sting cases were recorded in the state of São Paulo, between 2008 and 2018; there was a four-fold increase in the incidence rate. Accidents occurred more frequently in the spring season, wherein higher-risk clusters were in the north and northwest regions of the state. High temperatures, low precipitation, and poor natural vegetation are associated with higher risk areas. Our study mapped vulnerable areas for scorpion accidents that can aid in the design of efficient public health policies, which should be intensified during the spring season.

Revista da Sociedade Brasileira de Medicina Tropical, 2020

This reports a case of scorpionism caused by Tityus serrulatus. A male adult was stung while unlo... more This reports a case of scorpionism caused by Tityus serrulatus. A male adult was stung while unloading bananas at the supply center in Belém, Pará, Brazil. The bananas originated in another state (Bahia) and were brought to Belém by truck. The patient presented with pain, edema, and erythema at the sting site, and was classified as low-risk. The specimen was identified as T. serrulatus and symptomatic treatment and clinical observation were advised. The patient was discharged later without further complications. This is the first known envenomation caused by T. serrulatus, a non-native species to Pará, in the Brazilian Amazon.

Revista da Sociedade Brasileira de Medicina Tropical

We report the first pediatric case of a Jaguajir agamemnon scorpion sting. This case occurred in ... more We report the first pediatric case of a Jaguajir agamemnon scorpion sting. This case occurred in the state of Paraná and is the first record of this species in southern Brazil. The patient was admitted within 15 min, with pain, local edema, erythema, whole-body pruritus, and tongue paresthesia within the first two hours, which disappeared thereafter. The patient's condition was considered mild, with a positive outcome, and she recovered completely.

Waste Management, 2010

Studies of scorpion venoms have used different venom drying methods: lyophilization, desiccation,... more Studies of scorpion venoms have used different venom drying methods: lyophilization, desiccation, lyophilization after mixing with 0.9% saline or purified water and centrifugation. The aim of this study was to see if these different approaches cause some alteration in the composition of the venom or interfere with its biological effects. Mice were injected (i.p.) with T. serrulatus scorpion venom in the liquid form (G-liq) or dried by different methods (lyophilized-Glyo; centrifuged and the supernatant lyophilized-G-cen; desiccated-G-des), and observed regarding the occurrence of the symptoms respiratory difficulty, convulsion and death. The occurrence of seizures, although occurring in all groups and with the various doses used, did not prove to be effective to determine differences between the different handling techniques. Respiratory distress appeared to be useful in analyzing differences between groups, where this effect was less pronounced in the G-liq and G-des groups. In general, death occurred in a certain proportion with increasing dose for all groups. G-liq and Gdes seemed to be more "active" at lower doses and G-cen and G-lyo at higher doses. The electrophoretic and chromatographic profile demonstrated main differences between G-liq and the dried groups. In the electrophoretic profile, the liquid venom showed bands of proteins of higher concentration and greater number of major bands and the three dried venom had the lowest number of protein bands. The HPLC profile and densitometry of the electrophoretic profiles showed some differences that may be associated with different protein conformation/aggregation. Our data indicated that lyophilization is the most suitable method for processing T. serrulatus scorpion venom after extraction.

Zootaxa

Tityus martinpaechi Lourenço, 2001 was described based on a single adult female collected in the ... more Tityus martinpaechi Lourenço, 2001 was described based on a single adult female collected in the state of Ceará, Brazil. Subsequently, several scorpions of the genus Tityus were collected in the Chapada de Ibiapaba, Ceará, and sent to the Instituto Butantan in São Paulo, Brazil. One female and one male of these scorpions were identified as belonging to the species T. martinpaechi. Since the male of this species is yet unknown, it is described in this paper.

Tityus martinpaechi Lourenço, 2001 (Figs. 3 –12, Table 1) Holotype female from... more Tityus martinpaechi Lourenço, 2001 (Figs. 3 –12, Table 1) Holotype female from Francisco Saboia, State of Ceará, Brazil, deposited in the Museu Nacional (MNRJ, curator: A. B. Kury). Tityus martinpaechi Lourenço, 2001, Biogeographica 77 (1): 21–34. Distribution: Known only from the State of Ceará, Brazil. New record: Brazil, State of Ceará, Chapada de Ibiapaba (7 Km NW from Ipú), one male and one female, 6–16.XII. 2002, G. Skuk leg. (in reptile pitfall traps), deposited in IBSP 4502. Diagnosis Large buthids (female holotype 65.0; male 73.6); This species, with Tityus aba Candido, Lucas, Souza, Diaz & LiradaSilva, 2005 represents the largest species in the Tityus stigmurus complex. Tityus aba, and T. martinpaechi differ from remainig species of the T. stigmurus complex by presenting three longitudinal dorsal dark stripes along the mesosoma, the lateral ones reaching the VI tergite (fig. 1), and by having only one pair of spinoid granules on the dorsal keels of the I–IV metasomal segments (see Candido et al., 2005, fig. 5 and 6). From T. aba, it differs by having sparse dark spots on the legs and palps (fig. 1) and a carapace with only some dark spots (fig. 1) while in T.aba, the carapace is almost entire dark (see Candido et al., 2005, fig. 5). Description of male Coloration: Basically yellowish with many dark reddish spots (fig. 1 B). Carapace pale yellow with sparse brown spots (fig. 2); ocular tubercle dark. Mesosoma with three longitudinal dorsal stripes along tergites; the central stripe extends from the posterior edge of the carapace until the tergite VII while the lateral ones extend from the posterior edge of the carapace until the tergite VI. Metasoma segments I–IV yellow with dark brown spots on ventral and lateroventral carinae; the stain on segment V occupies all the posterior region (fig. 3 B). Vesicle yellow with no spots. Aculeus reddishyellow at its base and reddish at its extremity. Venter light yellow with no spots. Chelicera [...]

FIGURE 15. T. lamottei (holotype ♂ IBSP 1442; São Desidério, Bahia, Brazil) new synonym. Dorsal (... more FIGURE 15. T. lamottei (holotype ♂ IBSP 1442; São Desidério, Bahia, Brazil) new synonym. Dorsal (A) and ventral (B) view; Right palp in dorsal view (C); Metasomal segments in lateral view (D).

FIGURE 14. Distribution of T. serrulatus.

FIGURE 13. T. serrulatus. (♂ and Ψ IBSP 5046; Januária, Minas Gerais, Brazil). Metasomal segments... more FIGURE 13. T. serrulatus. (♂ and Ψ IBSP 5046; Januária, Minas Gerais, Brazil). Metasomal segments of the male in dorsal view (A); Metasomal segments II and IV in lateral view (B); Metasomal segments of the female in dorsal view (C).

FIGURE 12. T. serrulatus. (♂ and Ψ IBSP 5046; Januária, Minas Gerais, Brazil). Right palp in dors... more FIGURE 12. T. serrulatus. (♂ and Ψ IBSP 5046; Januária, Minas Gerais, Brazil). Right palp in dorsal view: female (A) and male (B). Scale bars = 5 mm.

FIGURE 11. T. serrulatus. (♂ and Ψ IBSP 5046; Januária, Minas Gerais, Brazil) Male in dorsal (A) ... more FIGURE 11. T. serrulatus. (♂ and Ψ IBSP 5046; Januária, Minas Gerais, Brazil) Male in dorsal (A) and ventral (B) view; Female in ventral (C) and dorsal (D) view.

Toxicon, 2019

Toxicon, Dec 1, 2018

Zenodo (CERN European Organization for Nuclear Research), Dec 31, 2006

Zenodo (CERN European Organization for Nuclear Research), Dec 31, 2009

PLOS Neglected Tropical Diseases, Jun 20, 2023

Journal of Venomous Animals and Toxins including Tropical Diseases

PLOS ONE

Acta Tropica, 2022

Revista da Sociedade Brasileira de Medicina Tropical, 2020

Revista da Sociedade Brasileira de Medicina Tropical

Waste Management, 2010

Studies of scorpion venoms have used different venom drying methods: lyophilization, desiccation,... more Studies of scorpion venoms have used different venom drying methods: lyophilization, desiccation, lyophilization after mixing with 0.9% saline or purified water and centrifugation. The aim of this study was to see if these different approaches cause some alteration in the composition of the venom or interfere with its biological effects. Mice were injected (i.p.) with T. serrulatus scorpion venom in the liquid form (G-liq) or dried by different methods (lyophilized-Glyo; centrifuged and the supernatant lyophilized-G-cen; desiccated-G-des), and observed regarding the occurrence of the symptoms respiratory difficulty, convulsion and death. The occurrence of seizures, although occurring in all groups and with the various doses used, did not prove to be effective to determine differences between the different handling techniques. Respiratory distress appeared to be useful in analyzing differences between groups, where this effect was less pronounced in the G-liq and G-des groups. In general, death occurred in a certain proportion with increasing dose for all groups. G-liq and Gdes seemed to be more "active" at lower doses and G-cen and G-lyo at higher doses. The electrophoretic and chromatographic profile demonstrated main differences between G-liq and the dried groups. In the electrophoretic profile, the liquid venom showed bands of proteins of higher concentration and greater number of major bands and the three dried venom had the lowest number of protein bands. The HPLC profile and densitometry of the electrophoretic profiles showed some differences that may be associated with different protein conformation/aggregation. Our data indicated that lyophilization is the most suitable method for processing T. serrulatus scorpion venom after extraction.

Zootaxa

FIGURE 14. Distribution of T. serrulatus.