Marcello Cantini - Academia.edu (original) (raw)

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Papers by Marcello Cantini

Research paper thumbnail of 774 Macrophage Cell Line Secrets a Specific Factor Capable of Inducing Myoblast Proliferation

Research paper thumbnail of Cell surface changes during muscle differentiation in vitro: A study with the probe 2,4,6-trinitrobenzene sulphonate

Cell Differentiation, 1979

Research paper thumbnail of Myosin types in cultured muscle cells

The Journal of cell biology, 1980

Fluorescent antibodies against fast skeletal, slow skeletal, and ventricular myosins were applied... more Fluorescent antibodies against fast skeletal, slow skeletal, and ventricular myosins were applied to muscle cultures from embryonic pectoralis and ventricular myocadium of the chicken. A number of spindle-shaped mononucleated cells, presumably myoblasts, and all myotubes present in skeletal muscle cultures were labeled by all three antimyosin antisera. In contrast, in cultures from ventricular myocardium all muscle cells were labeled by anti-ventricular myosin, whereas only part of them were stained by anti-slow skeletal myosin and rare cells reacted with anti-fast skeletal myosin. The findings indicate that myosin(s) present in cultured embryonic skeletal muscle cells contains antigenic determinants similar to those present in adult fast skeletal, slow skeletal, and ventricular myosins.

Research paper thumbnail of Macrophage-released Factor Stimulates Selectively Myogenic Cells in Primary Muscle Culture

Journal of Neuropathology and Experimental Neurology, 1995

Myofibers are reconstituted by the proliferation and fusion of muscle precursor cells when skelet... more Myofibers are reconstituted by the proliferation and fusion of muscle precursor cells when skeletal muscle is injured. One of the critical events is the peak accumulation of macrophages after 48 hours at the damage site before the satellite cell proliferation. In addition to their well-known role as a scavenger cell, there is now direct evidence of a mitogenic role of macrophages in regenerating muscle. We have utilized an in vitro model to directly investigate and prove that macrophages increase myoblast growth not only of satellite cells, but also of primary myoblasts. Rat muscle cells were cultured in the presence or absence of exudate macrophages obtained by peritoneal washing after thioglycollate broth injection. Macrophage coculture increases several times the myoblasts/myotubes yield. This effect is particularly evident in muscle culture conditions in which fibroblast growth is predominant over myoblast proliferation, suggesting a myoblast selective mitogenic effect of macrophages. The results are confirmed by quantitative analyses of both DNA and skeletal muscle-specific-contractile proteins by gel electrophoresis and immunocytochemistry. Experiments with macrophage-conditioned media show this effect is mediated by soluble factors. This growth factor-like activity, which has been shown to be acid-stable and heat-labile, labile, exerts its effects not only on specialized satellite cells during muscle regeneration, but also has a broader mitotic activity on all myogenic cells. In view of the role of muscle regeneration in muscle diseases and of the perspectives offered by gene therapy via myoblasts, we strongly believe that our results open new opportunities in removing many of the clinical constraints associated with repair and cell transplantation.

Research paper thumbnail of The Utrophin Gene Is Transcriptionally Up-regulated in Regenerating Muscle

Journal of Biological Chemistry, 2002

Research paper thumbnail of Effects of Pulsed Electromagnetic Fields on the Proliferation of Lymphocytes from Aids Patients, HIV-Seropositive Subjects, and Seronegative Drug Users

Journal of Bioelectricity, 1989

ABSTRACT The effect of the exposure of mitogen-stimulated lymphocytes from subjects infected by h... more ABSTRACT The effect of the exposure of mitogen-stimulated lymphocytes from subjects infected by human immunodeficiency virus to extremely low frequency pulsed electromagnetic fields (PEMFs) was studied, by evaluating the incorporation of tritiated thymidine, the expression of IL-2 receptor, and the amount of activated T lymphocytes. Four groups of subjects were considered patients with acquired immunodeficiency syndrome (AIDS), asymptomatic seropositive subjects, seronegative drug users, and young healthy controls. PEMFs increased cell proliferation only in the group of healthy controls, as measured at the 72nd hour of culture, but an increase in the number of activated T lymphocytes was observed by cytofluorimetric analysis after 18 hrs of PEMF exposure in cultures from AIDS patients.

Research paper thumbnail of Enhancing Effect of Low Frequency Pulsed Electromagnetic Fields on Lectin-Induced Human Lymphocyte Proliferation

Journal of Bioelectricity, 1986

The effect on cell proliferation of continuous exposure of human peripheral lymphocytes from a to... more The effect on cell proliferation of continuous exposure of human peripheral lymphocytes from a total of 19 subjects (14 healthy donors, 1 patient with muscular dystrophy (MD) and his 4 siblings) to pulsed extremely-low-frequency, low-intensity electromagnetic fields (PEMFs) was studied. PEWS were not mitogenic by themselves, and a slightly reduced 3H-thymidine (3H-TdR) incorporation in unstimulated lymphocyte cultures was observed. In phytohemagglutinin-stimulated lymphocyte cultures, increased 3H-TdR incorporation was ...

Research paper thumbnail of Exposure to low-frequency pulsed electromagnetic fields increases mitogen- induced lymphocyte proliferation in Down’s syndrome

Aging Clinical and Experimental Research, 1991

Research paper thumbnail of Dystrophin deficient myotubes undergo apoptosis in mouse primary muscle cell culture after DNA damage

Neuroscience Letters, 1998

Research paper thumbnail of 774 Macrophage Cell Line Secrets a Specific Factor Capable of Inducing Myoblast Proliferation

Research paper thumbnail of Cell surface changes during muscle differentiation in vitro: A study with the probe 2,4,6-trinitrobenzene sulphonate

Cell Differentiation, 1979

Research paper thumbnail of Myosin types in cultured muscle cells

The Journal of cell biology, 1980

Fluorescent antibodies against fast skeletal, slow skeletal, and ventricular myosins were applied... more Fluorescent antibodies against fast skeletal, slow skeletal, and ventricular myosins were applied to muscle cultures from embryonic pectoralis and ventricular myocadium of the chicken. A number of spindle-shaped mononucleated cells, presumably myoblasts, and all myotubes present in skeletal muscle cultures were labeled by all three antimyosin antisera. In contrast, in cultures from ventricular myocardium all muscle cells were labeled by anti-ventricular myosin, whereas only part of them were stained by anti-slow skeletal myosin and rare cells reacted with anti-fast skeletal myosin. The findings indicate that myosin(s) present in cultured embryonic skeletal muscle cells contains antigenic determinants similar to those present in adult fast skeletal, slow skeletal, and ventricular myosins.

Research paper thumbnail of Macrophage-released Factor Stimulates Selectively Myogenic Cells in Primary Muscle Culture

Journal of Neuropathology and Experimental Neurology, 1995

Myofibers are reconstituted by the proliferation and fusion of muscle precursor cells when skelet... more Myofibers are reconstituted by the proliferation and fusion of muscle precursor cells when skeletal muscle is injured. One of the critical events is the peak accumulation of macrophages after 48 hours at the damage site before the satellite cell proliferation. In addition to their well-known role as a scavenger cell, there is now direct evidence of a mitogenic role of macrophages in regenerating muscle. We have utilized an in vitro model to directly investigate and prove that macrophages increase myoblast growth not only of satellite cells, but also of primary myoblasts. Rat muscle cells were cultured in the presence or absence of exudate macrophages obtained by peritoneal washing after thioglycollate broth injection. Macrophage coculture increases several times the myoblasts/myotubes yield. This effect is particularly evident in muscle culture conditions in which fibroblast growth is predominant over myoblast proliferation, suggesting a myoblast selective mitogenic effect of macrophages. The results are confirmed by quantitative analyses of both DNA and skeletal muscle-specific-contractile proteins by gel electrophoresis and immunocytochemistry. Experiments with macrophage-conditioned media show this effect is mediated by soluble factors. This growth factor-like activity, which has been shown to be acid-stable and heat-labile, labile, exerts its effects not only on specialized satellite cells during muscle regeneration, but also has a broader mitotic activity on all myogenic cells. In view of the role of muscle regeneration in muscle diseases and of the perspectives offered by gene therapy via myoblasts, we strongly believe that our results open new opportunities in removing many of the clinical constraints associated with repair and cell transplantation.

Research paper thumbnail of The Utrophin Gene Is Transcriptionally Up-regulated in Regenerating Muscle

Journal of Biological Chemistry, 2002

Research paper thumbnail of Effects of Pulsed Electromagnetic Fields on the Proliferation of Lymphocytes from Aids Patients, HIV-Seropositive Subjects, and Seronegative Drug Users

Journal of Bioelectricity, 1989

ABSTRACT The effect of the exposure of mitogen-stimulated lymphocytes from subjects infected by h... more ABSTRACT The effect of the exposure of mitogen-stimulated lymphocytes from subjects infected by human immunodeficiency virus to extremely low frequency pulsed electromagnetic fields (PEMFs) was studied, by evaluating the incorporation of tritiated thymidine, the expression of IL-2 receptor, and the amount of activated T lymphocytes. Four groups of subjects were considered patients with acquired immunodeficiency syndrome (AIDS), asymptomatic seropositive subjects, seronegative drug users, and young healthy controls. PEMFs increased cell proliferation only in the group of healthy controls, as measured at the 72nd hour of culture, but an increase in the number of activated T lymphocytes was observed by cytofluorimetric analysis after 18 hrs of PEMF exposure in cultures from AIDS patients.

Research paper thumbnail of Enhancing Effect of Low Frequency Pulsed Electromagnetic Fields on Lectin-Induced Human Lymphocyte Proliferation

Journal of Bioelectricity, 1986

The effect on cell proliferation of continuous exposure of human peripheral lymphocytes from a to... more The effect on cell proliferation of continuous exposure of human peripheral lymphocytes from a total of 19 subjects (14 healthy donors, 1 patient with muscular dystrophy (MD) and his 4 siblings) to pulsed extremely-low-frequency, low-intensity electromagnetic fields (PEMFs) was studied. PEWS were not mitogenic by themselves, and a slightly reduced 3H-thymidine (3H-TdR) incorporation in unstimulated lymphocyte cultures was observed. In phytohemagglutinin-stimulated lymphocyte cultures, increased 3H-TdR incorporation was ...

Research paper thumbnail of Exposure to low-frequency pulsed electromagnetic fields increases mitogen- induced lymphocyte proliferation in Down’s syndrome

Aging Clinical and Experimental Research, 1991

Research paper thumbnail of Dystrophin deficient myotubes undergo apoptosis in mouse primary muscle cell culture after DNA damage

Neuroscience Letters, 1998