Carlo Masserdotti - Academia.edu (original) (raw)

Papers by Carlo Masserdotti

Veterinary Clinical Pathology, 2022

Journal of Comparative Pathology, 2018

Después de los domingos en que mi hermano nos visitaba, que yo terminé mi carrera universitaria e... more Después de los domingos en que mi hermano nos visitaba, que yo terminé mi carrera universitaria era el otro gran anhelo de mamá. Lo hice: terminé los estudios superiores. Pero mamá ya no está para verlo. Su memoria es todo lo que me queda. Sus recuerdos, que poco a poco se desvanecen, son aliento para continuar el camino. No le puedo dedicar este trabajo a un muerto, no soy un hipócrita, los muertos ya no ven, no hablan no escuchan, tal vez sienten. Estoy seguro, ella siente mi gratitud. Es más sincero agradecerle por las risas y enseñanzas que pasamos juntos. No sé si eso me hace buena persona, pero que me ánima a hacer una mejor cada día es seguro. Por el apoyo y la inspiración. Primer pensamiento, mejor pensamiento, siempre. Terminé con las bibliotecas Y las casas de putas Del norte de la ciudad, Nada me interesa. Cervantes, Baudelaire, Vallejo y Ginsberg Se marcharon en un viaje Del que nunca volverán. Mi madre también Partió en ese viaje. Gabriel Molina, 2013 iv Yo, Cristian Gabriel Cuenca Molina en calidad de autor del proyecto de investigación sobre "Paúl Watzlawick: teoría de la comunicación humana, una reflexión crítica sobre algunos de sus postulados", por la presente autorizo a la UNIVERSIDAD CENTRAL DEL ECUADOR, hacer uso de todos los contenidos que me pertenecen o de parte de los que contiene esta obra, con fines estrictamente académicos o de investigación. Los derechos que como autor me corresponden, con excepción de la presente autorización, seguirán vigentes a mi favor, de conformidad con lo establecido en los artículos 5,6,8,

Veterinary Clinical Pathology

BACKGROUND Histologic examination, together with clinical data and instrument support, contribute... more BACKGROUND Histologic examination, together with clinical data and instrument support, contributes to the recognition of biliary diseases. Although quantitative and qualitative changes in hepatic and biliary cells can occur, descriptions of cytologic samples from the liver affected by cholangiopathies are rare. OBJECTIVES This study aimed to describe the quantitative and qualitative changes in cholangiocytes, hepatocytes, and inflammatory cells in cytologic samples from dogs and cats affected by histologically confirmed cholangiopathies. METHODS Cytologic samples from the liver with histologically confirmed cholangiopathies were reviewed and compared to describe the changes seen on cytology. The samples were obtained from the Archive of Clinica Veterinaria Sant'Antonio and the Laboratorio Veterinario Bresciano. Statistical analysis was not performed. RESULTS A total of 196 cytologic samples retrieved from the database fit the selection criteria, 88 from dogs, and 108 from cats. A total of eight categories of cholangiopathies have been identified and described, while histology is often required to achieve a definitive diagnosis, six categories of cholangiopathy in dogs and seven in cats provide cytologic information. CONCLUSIONS Cytologic examination, together with clinical and ultrasonographic data, can be useful for identifying hepatic abnormalities in acute and chronic cholestasis, chronic cholangitis, feline lymphoplasmacytic cholangitis, and cholangiocellular neoplasms.

Veterinary clinical pathology, Jan 5, 2017

Journal of Comparative Pathology, 2017

Veterinary clinical pathology / American Society for Veterinary Clinical Pathology, Jan 9, 2016

Liver cytology has diagnostic value for the identification of neoplastic and nonneoplastic hepati... more Liver cytology has diagnostic value for the identification of neoplastic and nonneoplastic hepatic diseases. However, so far the diagnosis of fibrotic changes has traditionally been restricted to histopathology. The aim of this study was to describe the cytologic features that may help in the recognition of hepatic fibrosis. Cytologic smears from the liver of dogs histologically diagnosed with hepatic fibrosis (Group A) were selected and compared to liver smears from dogs without hepatic fibrosis (Group B). A differential count of hepatocytes, spindle cells, mast cells, granulocytes, lymphocytes/plasma cells, and macrophages was documented for each case, and means for the fibrosis and nonfibrosis group were compared. The relative and total numbers of spindle cells and mast cells were significantly higher in the fibrosis group than in the nonfibrosis group. The optimal cutoff point for the spindle cells to hepatocytes ratio was 0.107 (ie, one spindle cell per 10 hepatocytes), with 95...

Journal of feline medicine and surgery, Jan 2, 2016

The aim of this study was to investigate the role of thyroid transcription factor-1 (TTF-1) in th... more The aim of this study was to investigate the role of thyroid transcription factor-1 (TTF-1) in the diagnosis of feline lung digit syndrome (FLDS) and to investigate the associations between the morphological features of FLDS and TTF-1 expression. We also compared the reliability of TTF-1 and transmission electron microscopy (TEM) in establishing the diagnosis of FLDS. Histology records of feline digit tumours were retrieved, including patients from 2008-2015. If formalin-fixed, paraffin-embedded tissues were available for review, patients were included in the study. As a control group we included 12 feline primary tumours of the digits. All the histological slides of the study group were blindly reviewed by the same veterinary pathologist. Representative sections of the lesions were selected for immunohistochemistry (IHC) analysis. To confirm the respiratory origin of the neoplastic tissue, TEM was used as a gold standard in all cases. Five cases of FLDS were included. TTF-1 was wea...

Veterinary Clinical Pathology, 2016

Canine and Feline Cytology, 2016

Veterinary Clinical Pathology, 2009

Northern Italy is a habitat for many species of viper; Vipera aspis is responsible for most repor... more Northern Italy is a habitat for many species of viper; Vipera aspis is responsible for most reported bites of humans and animals. Five dogs of different breeds and ages were presented by their owners to a veterinary clinic in northern Italy between September 2004 and August 2007 with a history of being bitten by a viper within the past 2 hours. On physical examination, all of the dogs were depressed, had pale mucous membranes, and had a painful area consistent with a recent viper bite wound on the nose (2), distal front leg (2), or lip (1). Hemoglobinemia and hemoglobinuria were observed in plasma and urine from all dogs. CBCs were done at the time of presentation, daily for 4 days, and 10 days after presentation in all dogs with the exception of one dog that died after 2 days. All dogs had an acute decrease in HCT within 24 hours of presentation; all dogs had neutrophilia, 3/5 had a mildly toxic left shift, and 4/5 had thrombocytopenia. On Diff-Quik-stained blood smears, moderate numbers of echinocytes, spherocytes, and erythrocyte ghosts were observed. In addition, moderate numbers of unusual erythrocyte membrane-like structures ("erythroid loops") were observed. The loops were annular in shape and sometimes disrupted, appearing as thin pale blue bands. Erythrocyte morphologic abnormalities decreased by day 3 and were no longer observed on day 10. The unique appearance of the erythroid loops together with evidence for intravascular hemolysis and other erythrocyte morphologic changes suggest they may be a consequence of erythrocyte lysis. Echinocytes, spherocytes, and erythrocyte ghosts are known to result from the action of phospholipase in viper venom; however, erythroid loops have not been reported previously and their exact mechanism of formation is unknown.

Veterinary Clinical Pathology, 2009

Veterinary Clinical Pathology, 2008

Masserdotti et al Cytologic Detection of Call-Exner Bodies

Veterinary Clinical Pathology, 2012

Cytologic evaluation plays an important role in the diagnosis of ovarian neoplasia in dogs, but i... more Cytologic evaluation plays an important role in the diagnosis of ovarian neoplasia in dogs, but is supported by only scant information on cytologic features of canine ovaries. The aim of this study was to provide detailed cytologic descriptions of normal canine ovaries in different stages of estrus with comparison to histologic features. Client-owned dogs admitted for elective ovariohysterectomy were studied. For each dog, fine-needle aspirates of both ovaries were collected, stained, and examined and were compared with histologic sections of the same ovary. The stage of estrus was confirmed by examination of histologic sections and cytologic specimens of vaginal cells collected with swabs. Thirty-two ovaries from 16 dogs were examined. Luteal cells were observed in 82% of the dogs in diestrus. In early diestrus these cells were polygonal with amphophilic to deeply basophilic cytoplasm, and in late diestrus luteal cells had blebbed cell borders and clear cytoplasm with numerous small vacuoles. Perivascular arrangements and leuko-emperipolesis were noted in both phases of diestrus. Granulosa cells and spindle cells were found in cytologic specimens from most of the ovaries, and blue-gray extracellular material, sometimes associated with granulosa cells, was present. Medium-sized discrete round cells of undetermined origin were observed in some stages of estrus, and structures classified as corpora albicans were noted occasionally. Knowledge of specific cytologic features of normal canine ovaries is important for identification of pathologic processes in this organ. The novel findings of luteal cell emperipolesis, extracellular material associated with granulosa cells, and round cells of undetermined lineage warrant further study, which may provide new information on canine ovarian structure and function.

Veterinary Clinical Pathology, 2012

Veterinary Clinical Pathology, 2013

Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the ... more Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the normal proportion of these cells in cytologic specimens from normal livers was not previously established. The objective of this study was to determine the normal proportion of hepatic mast cells in cytologic specimens from normal canine liver by the use and comparison of the May-Grünwald-Giemsa and the Toluidine Blue staining methods. The cytologic specimens obtained from fine-needle aspirates of livers of 13 healthy dogs were investigated by counting of hepatocytes and mast cells per high-power field (HPF, x40 objective). The ratio of mast cells/100 hepatocytes was then compared between the 2 staining methods. A median of 0.17 (range: 0-0.33) mast cells/100 hepatocytes and of 0.47 (range: 0-0.86) mast cells/100 hepatocytes was obtained with May-Grünwald-Giemsa and Toluidine Blue stains, respectively. Toluidine Blue stain detects more hepatic mast cells than May-Grunwald-Giemsa in normal canine livers. With both stains, < 1% mast cells were detected in normal canine liver samples.

Veterinary Clinical Pathology, 2022

Journal of Comparative Pathology, 2018

Después de los domingos en que mi hermano nos visitaba, que yo terminé mi carrera universitaria e... more Después de los domingos en que mi hermano nos visitaba, que yo terminé mi carrera universitaria era el otro gran anhelo de mamá. Lo hice: terminé los estudios superiores. Pero mamá ya no está para verlo. Su memoria es todo lo que me queda. Sus recuerdos, que poco a poco se desvanecen, son aliento para continuar el camino. No le puedo dedicar este trabajo a un muerto, no soy un hipócrita, los muertos ya no ven, no hablan no escuchan, tal vez sienten. Estoy seguro, ella siente mi gratitud. Es más sincero agradecerle por las risas y enseñanzas que pasamos juntos. No sé si eso me hace buena persona, pero que me ánima a hacer una mejor cada día es seguro. Por el apoyo y la inspiración. Primer pensamiento, mejor pensamiento, siempre. Terminé con las bibliotecas Y las casas de putas Del norte de la ciudad, Nada me interesa. Cervantes, Baudelaire, Vallejo y Ginsberg Se marcharon en un viaje Del que nunca volverán. Mi madre también Partió en ese viaje. Gabriel Molina, 2013 iv Yo, Cristian Gabriel Cuenca Molina en calidad de autor del proyecto de investigación sobre "Paúl Watzlawick: teoría de la comunicación humana, una reflexión crítica sobre algunos de sus postulados", por la presente autorizo a la UNIVERSIDAD CENTRAL DEL ECUADOR, hacer uso de todos los contenidos que me pertenecen o de parte de los que contiene esta obra, con fines estrictamente académicos o de investigación. Los derechos que como autor me corresponden, con excepción de la presente autorización, seguirán vigentes a mi favor, de conformidad con lo establecido en los artículos 5,6,8,

Veterinary Clinical Pathology

BACKGROUND Histologic examination, together with clinical data and instrument support, contribute... more BACKGROUND Histologic examination, together with clinical data and instrument support, contributes to the recognition of biliary diseases. Although quantitative and qualitative changes in hepatic and biliary cells can occur, descriptions of cytologic samples from the liver affected by cholangiopathies are rare. OBJECTIVES This study aimed to describe the quantitative and qualitative changes in cholangiocytes, hepatocytes, and inflammatory cells in cytologic samples from dogs and cats affected by histologically confirmed cholangiopathies. METHODS Cytologic samples from the liver with histologically confirmed cholangiopathies were reviewed and compared to describe the changes seen on cytology. The samples were obtained from the Archive of Clinica Veterinaria Sant'Antonio and the Laboratorio Veterinario Bresciano. Statistical analysis was not performed. RESULTS A total of 196 cytologic samples retrieved from the database fit the selection criteria, 88 from dogs, and 108 from cats. A total of eight categories of cholangiopathies have been identified and described, while histology is often required to achieve a definitive diagnosis, six categories of cholangiopathy in dogs and seven in cats provide cytologic information. CONCLUSIONS Cytologic examination, together with clinical and ultrasonographic data, can be useful for identifying hepatic abnormalities in acute and chronic cholestasis, chronic cholangitis, feline lymphoplasmacytic cholangitis, and cholangiocellular neoplasms.

Veterinary clinical pathology, Jan 5, 2017

Journal of Comparative Pathology, 2017

Veterinary clinical pathology / American Society for Veterinary Clinical Pathology, Jan 9, 2016

Liver cytology has diagnostic value for the identification of neoplastic and nonneoplastic hepati... more Liver cytology has diagnostic value for the identification of neoplastic and nonneoplastic hepatic diseases. However, so far the diagnosis of fibrotic changes has traditionally been restricted to histopathology. The aim of this study was to describe the cytologic features that may help in the recognition of hepatic fibrosis. Cytologic smears from the liver of dogs histologically diagnosed with hepatic fibrosis (Group A) were selected and compared to liver smears from dogs without hepatic fibrosis (Group B). A differential count of hepatocytes, spindle cells, mast cells, granulocytes, lymphocytes/plasma cells, and macrophages was documented for each case, and means for the fibrosis and nonfibrosis group were compared. The relative and total numbers of spindle cells and mast cells were significantly higher in the fibrosis group than in the nonfibrosis group. The optimal cutoff point for the spindle cells to hepatocytes ratio was 0.107 (ie, one spindle cell per 10 hepatocytes), with 95...

Journal of feline medicine and surgery, Jan 2, 2016

The aim of this study was to investigate the role of thyroid transcription factor-1 (TTF-1) in th... more The aim of this study was to investigate the role of thyroid transcription factor-1 (TTF-1) in the diagnosis of feline lung digit syndrome (FLDS) and to investigate the associations between the morphological features of FLDS and TTF-1 expression. We also compared the reliability of TTF-1 and transmission electron microscopy (TEM) in establishing the diagnosis of FLDS. Histology records of feline digit tumours were retrieved, including patients from 2008-2015. If formalin-fixed, paraffin-embedded tissues were available for review, patients were included in the study. As a control group we included 12 feline primary tumours of the digits. All the histological slides of the study group were blindly reviewed by the same veterinary pathologist. Representative sections of the lesions were selected for immunohistochemistry (IHC) analysis. To confirm the respiratory origin of the neoplastic tissue, TEM was used as a gold standard in all cases. Five cases of FLDS were included. TTF-1 was wea...

Veterinary Clinical Pathology, 2016

Canine and Feline Cytology, 2016

Veterinary Clinical Pathology, 2009

Northern Italy is a habitat for many species of viper; Vipera aspis is responsible for most repor... more Northern Italy is a habitat for many species of viper; Vipera aspis is responsible for most reported bites of humans and animals. Five dogs of different breeds and ages were presented by their owners to a veterinary clinic in northern Italy between September 2004 and August 2007 with a history of being bitten by a viper within the past 2 hours. On physical examination, all of the dogs were depressed, had pale mucous membranes, and had a painful area consistent with a recent viper bite wound on the nose (2), distal front leg (2), or lip (1). Hemoglobinemia and hemoglobinuria were observed in plasma and urine from all dogs. CBCs were done at the time of presentation, daily for 4 days, and 10 days after presentation in all dogs with the exception of one dog that died after 2 days. All dogs had an acute decrease in HCT within 24 hours of presentation; all dogs had neutrophilia, 3/5 had a mildly toxic left shift, and 4/5 had thrombocytopenia. On Diff-Quik-stained blood smears, moderate numbers of echinocytes, spherocytes, and erythrocyte ghosts were observed. In addition, moderate numbers of unusual erythrocyte membrane-like structures ("erythroid loops") were observed. The loops were annular in shape and sometimes disrupted, appearing as thin pale blue bands. Erythrocyte morphologic abnormalities decreased by day 3 and were no longer observed on day 10. The unique appearance of the erythroid loops together with evidence for intravascular hemolysis and other erythrocyte morphologic changes suggest they may be a consequence of erythrocyte lysis. Echinocytes, spherocytes, and erythrocyte ghosts are known to result from the action of phospholipase in viper venom; however, erythroid loops have not been reported previously and their exact mechanism of formation is unknown.

Veterinary Clinical Pathology, 2009

Veterinary Clinical Pathology, 2008

Masserdotti et al Cytologic Detection of Call-Exner Bodies

Veterinary Clinical Pathology, 2012

Cytologic evaluation plays an important role in the diagnosis of ovarian neoplasia in dogs, but i... more Cytologic evaluation plays an important role in the diagnosis of ovarian neoplasia in dogs, but is supported by only scant information on cytologic features of canine ovaries. The aim of this study was to provide detailed cytologic descriptions of normal canine ovaries in different stages of estrus with comparison to histologic features. Client-owned dogs admitted for elective ovariohysterectomy were studied. For each dog, fine-needle aspirates of both ovaries were collected, stained, and examined and were compared with histologic sections of the same ovary. The stage of estrus was confirmed by examination of histologic sections and cytologic specimens of vaginal cells collected with swabs. Thirty-two ovaries from 16 dogs were examined. Luteal cells were observed in 82% of the dogs in diestrus. In early diestrus these cells were polygonal with amphophilic to deeply basophilic cytoplasm, and in late diestrus luteal cells had blebbed cell borders and clear cytoplasm with numerous small vacuoles. Perivascular arrangements and leuko-emperipolesis were noted in both phases of diestrus. Granulosa cells and spindle cells were found in cytologic specimens from most of the ovaries, and blue-gray extracellular material, sometimes associated with granulosa cells, was present. Medium-sized discrete round cells of undetermined origin were observed in some stages of estrus, and structures classified as corpora albicans were noted occasionally. Knowledge of specific cytologic features of normal canine ovaries is important for identification of pathologic processes in this organ. The novel findings of luteal cell emperipolesis, extracellular material associated with granulosa cells, and round cells of undetermined lineage warrant further study, which may provide new information on canine ovarian structure and function.

Veterinary Clinical Pathology, 2012

Veterinary Clinical Pathology, 2013

Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the ... more Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the normal proportion of these cells in cytologic specimens from normal livers was not previously established. The objective of this study was to determine the normal proportion of hepatic mast cells in cytologic specimens from normal canine liver by the use and comparison of the May-Grünwald-Giemsa and the Toluidine Blue staining methods. The cytologic specimens obtained from fine-needle aspirates of livers of 13 healthy dogs were investigated by counting of hepatocytes and mast cells per high-power field (HPF, x40 objective). The ratio of mast cells/100 hepatocytes was then compared between the 2 staining methods. A median of 0.17 (range: 0-0.33) mast cells/100 hepatocytes and of 0.47 (range: 0-0.86) mast cells/100 hepatocytes was obtained with May-Grünwald-Giemsa and Toluidine Blue stains, respectively. Toluidine Blue stain detects more hepatic mast cells than May-Grunwald-Giemsa in normal canine livers. With both stains, < 1% mast cells were detected in normal canine liver samples.