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Papers by Carlo Nebbia

Research paper thumbnail of SCIENTIFIC OPINION Safety and efficacy of the product Ronozyme ® NP (6-phytase) for use as feed additive for poultry, weaned piglets and pigs for fattening 1 Scientific Opinion of the Panel on Additives and Products or Substances used in Animal Feed

SUMMARY Following a request from the European Commission, the European Food Safety Authority (EFS... more SUMMARY Following a request from the European Commission, the European Food Safety Authority (EFSA) was asked to deliver a scientific opinion on the safety and efficacy of Toyocerin ® (Bacillus cereus) used as a feed additive for rabbit breeding does. Toyocerin ® is a zootechnical additive composed of spores of a strain of Bacillus cereus mixed with maize flour and calcium carbonate. The product has already been granted permanent or provisional authorisation for use with most livestock species and categories. In particular, Toyocerin ® is already permanently authorised for use with rabbits for fattening and was provisionally authorised for use with breeding does. The applicant is now seeking authorisation for a period of ten years for the continuing use of Toyocerin ® in diets for rabbit breeding does from service to weaning of kits (new-born rabbits). The recommended inclusion level is 0.2 – 1.0 x 10 9 CFU kg -1 complete feedingstuff, with the added condition that it may be used in compound feed containing the permitted coccidiostats robenidine and salinomycin sodium. The safety of Toyocerin ® for consumers of food products derived from animals fed diets containing Toyocerin ® , for users of the product and for the wider environment has been established as part of the previous authorisations. The FEEDAP Panel is not aware of any additional data which would require a reconsideration of those safety and practical 1 For citation purposes: Scientific Opinion of the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) on a request from the European Commission on the safety and efficacy of the product Toyocerin ® (Bacillus

Research paper thumbnail of Preliminary evaluation of the safety and efficacy of paromomycin sulphate for turkeys for fattening and turkeys reared for breeding 1 Scientific Opinion of the Panel on Additives and Products or Substances used in Animal Feed

Research paper thumbnail of A preliminary investigation on the effects of quercetin on aflatoxin B1 (AFB1) toxicity and transport in an in vitro model of functional Caco-2 intestinal cells

HAL (Le Centre pour la Communication Scientifique Directe), Jun 24, 2018

Research paper thumbnail of Changes in serum low molecular weight proteins from DL-PCB contaminated heifers

HAL (Le Centre pour la Communication Scientifique Directe), Jul 19, 2015

Research paper thumbnail of Serum antioxidant status and oxidative stress parameters as biomarkers for illicit treatments with growth promoting agents in veal calves

Research paper thumbnail of Effects of triphenyltin acetate on lymphopoiethic system and hepatic and renal cytochrome P450 content in rabbits

Research paper thumbnail of Decontamination process for dioxins and dioxin‐like PCBs from fish oil and vegetable oils and fats by a physical process with activated carbon

Research paper thumbnail of Effects of natural antioxidants and pentachlorobiphenyl (PCB126) on the modulation of aflatoxin B1 (AFB1) cytotoxicity and metabolism in a bovine mammary epithelial cell line

14th International Congress of EAVPT, 2018

Research paper thumbnail of Residui di farmaci nelle derrate di origine animale

Research paper thumbnail of Curcumin Supplementation Protects Broiler Chickens Against the Renal Oxidative Stress Induced by the Dietary Exposure to Low Levels of Aflatoxin B1

Frontiers in Veterinary Science, 2022

Aflatoxin B1 (AFB1) causes hepatotoxicity, immunotoxicity, and kidney damage, and it is included ... more Aflatoxin B1 (AFB1) causes hepatotoxicity, immunotoxicity, and kidney damage, and it is included in group I of human carcinogens. The European Commission has established maximum limits of AFB1 in feed, ranging from 5 to 20 μg/kg. Chicken is moderately sensitive to AFB1, which results in reduced growth performance and economic losses. Oxidative stress triggered by AFB1 plays a crucial role in kidney damage and the antioxidant activity of Curcumin (CURC) could help in preventing such adverse effect. Twenty-days-old broilers were treated for 10 days with AFB1 (0.02 mg/kg feed), alone or in combination with CURC (400 mg/kg feed), to explore the effects on the renal tissue. Animals exposed to AFB1 alone displayed alterations of the oxidative stress parameters compared with controls: serum antioxidant capacity, and enzymatic activity of kidney superoxide dismutase, catalase and glutathione peroxidase were decreased, while renal malondialdehyde levels and NADPH oxidase complex expression w...

Research paper thumbnail of Assessment of an application on a detoxification process of groundnut press cake for aflatoxins by ammoniation

EFSA Journal, 2021

Abstract Following a request from the European Commission, the EFSA Panel on Contaminants in the ... more Abstract Following a request from the European Commission, the EFSA Panel on Contaminants in the Food Chain (CONTAM) provided a scientific opinion on an application for a detoxification process of groundnut press cake for aflatoxins by ammoniation. Specifically, it is required that the feed decontamination process is compliant with the acceptability criteria specified in the Commission Regulation (EU) 2015/786 of 19 May 2015. The CONTAM Panel assessed the data provided by the feed business operator with respect to the efficacy of the process to remove the contaminant from groundnut press cake batches and on information demonstrating that the process does not adversely affect the characteristics and the nature of the product. Although according to the literature the process may be able to reduce aflatoxin levels below the legal limits, the Panel concluded that the proposed decontamination process, on the basis of the experimental data submitted by the feed business operator, cannot be confirmed for compliance with the acceptability criteria provided for in Commission Regulation (EU) 2015/786 of 19 May 2015. The Panel recommended sufficient sample testing before and after the process, under the selected conditions, to ensure that the process is reproducible and reliable and to demonstrate that the detoxification is not reversible. In addition, genotoxicity testing of extracts of the treated feedingstuff and of the identified degradation products would be necessary. Finally, information on the transfer rate of AFB1 to AFM1 excretion in milk for animals fed the ammoniated product, in comparison to the starting material and on the ammoniation process changes of the nutritional values of the feed material should be provided.

Research paper thumbnail of Screening evaluation of growth-promoter abuse in veal calves using serum biomarkers

Journal of Veterinary Pharmacology and Therapeutics, 2015

Research paper thumbnail of Preliminary analyses of biomarkers of the exposure to dioxins and dioxin-like substances in dairy cattle

Italian Journal of Animal Science, 2009

Several investigations have recently searched for significant association between gene polymorphi... more Several investigations have recently searched for significant association between gene polymorphisms and milk traits in livestock and model species. In several cases, it remains rather difficult to assess if the observed effects are caused by the mutation tested, by a nearby mutation in the same gene or by a mutation in a different gene or DNA region in linkage disequilibrium with the former. As a consequence, only in a few cases (e.g., κ-casein, SCD, DGAT1) the causative mutation seems to have been identified and, even when evidence is rather clear, genetic heterogeneity and genetic background may influence the size of allele substitution effects. Therefore, the significance of gene-trait associations and the estimate of their effect have to be verified in any new population in which this information is planned to be used, to estimate its actual utility in gene assisted breeding. In the SelMol project, we selected 29 candidate genes on the basis of known relationships between physi...

Research paper thumbnail of Safety and efficacy of sodium carboxymethyl cellulose for all animal species

Research paper thumbnail of Safety and efficacy of microcrystalline cellulose for all animal species

Research paper thumbnail of Safety and efficacy of ethyl cellulose for all animal species

Research paper thumbnail of Safety and efficacy of methyl cellulose for all animal species

Research paper thumbnail of Efficacy of calcium formate as a technological feed additive (preservative) for all animal species

Research paper thumbnail of Safety of hexamethylene tetramine for pigs, poultry, bovines, sheep, goats, rabbits and horses

Research paper thumbnail of Assessment of a decontamination process for hydrocyanic acid in linseed intended for use in animal feed

Research paper thumbnail of SCIENTIFIC OPINION Safety and efficacy of the product Ronozyme ® NP (6-phytase) for use as feed additive for poultry, weaned piglets and pigs for fattening 1 Scientific Opinion of the Panel on Additives and Products or Substances used in Animal Feed

SUMMARY Following a request from the European Commission, the European Food Safety Authority (EFS... more SUMMARY Following a request from the European Commission, the European Food Safety Authority (EFSA) was asked to deliver a scientific opinion on the safety and efficacy of Toyocerin ® (Bacillus cereus) used as a feed additive for rabbit breeding does. Toyocerin ® is a zootechnical additive composed of spores of a strain of Bacillus cereus mixed with maize flour and calcium carbonate. The product has already been granted permanent or provisional authorisation for use with most livestock species and categories. In particular, Toyocerin ® is already permanently authorised for use with rabbits for fattening and was provisionally authorised for use with breeding does. The applicant is now seeking authorisation for a period of ten years for the continuing use of Toyocerin ® in diets for rabbit breeding does from service to weaning of kits (new-born rabbits). The recommended inclusion level is 0.2 – 1.0 x 10 9 CFU kg -1 complete feedingstuff, with the added condition that it may be used in compound feed containing the permitted coccidiostats robenidine and salinomycin sodium. The safety of Toyocerin ® for consumers of food products derived from animals fed diets containing Toyocerin ® , for users of the product and for the wider environment has been established as part of the previous authorisations. The FEEDAP Panel is not aware of any additional data which would require a reconsideration of those safety and practical 1 For citation purposes: Scientific Opinion of the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) on a request from the European Commission on the safety and efficacy of the product Toyocerin ® (Bacillus

Research paper thumbnail of Preliminary evaluation of the safety and efficacy of paromomycin sulphate for turkeys for fattening and turkeys reared for breeding 1 Scientific Opinion of the Panel on Additives and Products or Substances used in Animal Feed

Research paper thumbnail of A preliminary investigation on the effects of quercetin on aflatoxin B1 (AFB1) toxicity and transport in an in vitro model of functional Caco-2 intestinal cells

HAL (Le Centre pour la Communication Scientifique Directe), Jun 24, 2018

Research paper thumbnail of Changes in serum low molecular weight proteins from DL-PCB contaminated heifers

HAL (Le Centre pour la Communication Scientifique Directe), Jul 19, 2015

Research paper thumbnail of Serum antioxidant status and oxidative stress parameters as biomarkers for illicit treatments with growth promoting agents in veal calves

Research paper thumbnail of Effects of triphenyltin acetate on lymphopoiethic system and hepatic and renal cytochrome P450 content in rabbits

Research paper thumbnail of Decontamination process for dioxins and dioxin‐like PCBs from fish oil and vegetable oils and fats by a physical process with activated carbon

Research paper thumbnail of Effects of natural antioxidants and pentachlorobiphenyl (PCB126) on the modulation of aflatoxin B1 (AFB1) cytotoxicity and metabolism in a bovine mammary epithelial cell line

14th International Congress of EAVPT, 2018

Research paper thumbnail of Residui di farmaci nelle derrate di origine animale

Research paper thumbnail of Curcumin Supplementation Protects Broiler Chickens Against the Renal Oxidative Stress Induced by the Dietary Exposure to Low Levels of Aflatoxin B1

Frontiers in Veterinary Science, 2022

Aflatoxin B1 (AFB1) causes hepatotoxicity, immunotoxicity, and kidney damage, and it is included ... more Aflatoxin B1 (AFB1) causes hepatotoxicity, immunotoxicity, and kidney damage, and it is included in group I of human carcinogens. The European Commission has established maximum limits of AFB1 in feed, ranging from 5 to 20 μg/kg. Chicken is moderately sensitive to AFB1, which results in reduced growth performance and economic losses. Oxidative stress triggered by AFB1 plays a crucial role in kidney damage and the antioxidant activity of Curcumin (CURC) could help in preventing such adverse effect. Twenty-days-old broilers were treated for 10 days with AFB1 (0.02 mg/kg feed), alone or in combination with CURC (400 mg/kg feed), to explore the effects on the renal tissue. Animals exposed to AFB1 alone displayed alterations of the oxidative stress parameters compared with controls: serum antioxidant capacity, and enzymatic activity of kidney superoxide dismutase, catalase and glutathione peroxidase were decreased, while renal malondialdehyde levels and NADPH oxidase complex expression w...

Research paper thumbnail of Assessment of an application on a detoxification process of groundnut press cake for aflatoxins by ammoniation

EFSA Journal, 2021

Abstract Following a request from the European Commission, the EFSA Panel on Contaminants in the ... more Abstract Following a request from the European Commission, the EFSA Panel on Contaminants in the Food Chain (CONTAM) provided a scientific opinion on an application for a detoxification process of groundnut press cake for aflatoxins by ammoniation. Specifically, it is required that the feed decontamination process is compliant with the acceptability criteria specified in the Commission Regulation (EU) 2015/786 of 19 May 2015. The CONTAM Panel assessed the data provided by the feed business operator with respect to the efficacy of the process to remove the contaminant from groundnut press cake batches and on information demonstrating that the process does not adversely affect the characteristics and the nature of the product. Although according to the literature the process may be able to reduce aflatoxin levels below the legal limits, the Panel concluded that the proposed decontamination process, on the basis of the experimental data submitted by the feed business operator, cannot be confirmed for compliance with the acceptability criteria provided for in Commission Regulation (EU) 2015/786 of 19 May 2015. The Panel recommended sufficient sample testing before and after the process, under the selected conditions, to ensure that the process is reproducible and reliable and to demonstrate that the detoxification is not reversible. In addition, genotoxicity testing of extracts of the treated feedingstuff and of the identified degradation products would be necessary. Finally, information on the transfer rate of AFB1 to AFM1 excretion in milk for animals fed the ammoniated product, in comparison to the starting material and on the ammoniation process changes of the nutritional values of the feed material should be provided.

Research paper thumbnail of Screening evaluation of growth-promoter abuse in veal calves using serum biomarkers

Journal of Veterinary Pharmacology and Therapeutics, 2015

Research paper thumbnail of Preliminary analyses of biomarkers of the exposure to dioxins and dioxin-like substances in dairy cattle

Italian Journal of Animal Science, 2009

Several investigations have recently searched for significant association between gene polymorphi... more Several investigations have recently searched for significant association between gene polymorphisms and milk traits in livestock and model species. In several cases, it remains rather difficult to assess if the observed effects are caused by the mutation tested, by a nearby mutation in the same gene or by a mutation in a different gene or DNA region in linkage disequilibrium with the former. As a consequence, only in a few cases (e.g., κ-casein, SCD, DGAT1) the causative mutation seems to have been identified and, even when evidence is rather clear, genetic heterogeneity and genetic background may influence the size of allele substitution effects. Therefore, the significance of gene-trait associations and the estimate of their effect have to be verified in any new population in which this information is planned to be used, to estimate its actual utility in gene assisted breeding. In the SelMol project, we selected 29 candidate genes on the basis of known relationships between physi...

Research paper thumbnail of Safety and efficacy of sodium carboxymethyl cellulose for all animal species

Research paper thumbnail of Safety and efficacy of microcrystalline cellulose for all animal species

Research paper thumbnail of Safety and efficacy of ethyl cellulose for all animal species

Research paper thumbnail of Safety and efficacy of methyl cellulose for all animal species

Research paper thumbnail of Efficacy of calcium formate as a technological feed additive (preservative) for all animal species

Research paper thumbnail of Safety of hexamethylene tetramine for pigs, poultry, bovines, sheep, goats, rabbits and horses

Research paper thumbnail of Assessment of a decontamination process for hydrocyanic acid in linseed intended for use in animal feed

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