Carlo Patriarca - Academia.edu (original) (raw)
Papers by Carlo Patriarca
Tumori
In order to study the possible relationship between antigenic phenotype and tumor progression, 63... more In order to study the possible relationship between antigenic phenotype and tumor progression, 63 small cell lung carcinomas (SCLC) biopsies derived from primary or metastatic tumors were tested by immunofluorescence and immunoperoxidase techniques with an anti-carcinoma monoclonal antibody designated MBr1. Primary tumors were found to be less reactive with MBr1 than the local and distant metastatic lesions (57% versus 75% and 89% positivity respectively). A life table analysis on the tested cases indicated an inverse association between the expression of the marker recognized by the MBr1 MAb (CaMBr1) and overall survival (p less than 0.01): patients with MBr1-positive tumors showed a shorter survival time in comparison to patients whose tumors did not express the marker. The same correlation between survival and CaMBr1 expression was found even when only the 31 cases of early stage disease patients were considered (p less than 0.05). Different tumor aggressiveness or resistance to therapy of MBr1-positive tumors could be responsible for the shorter survival time of the patients.
Tumori
The clinico-pathologic characteristics of 10 resected pulmonary tumors, which proved to be well-d... more The clinico-pathologic characteristics of 10 resected pulmonary tumors, which proved to be well-differentiated neuroendocrine carcinomas (WDNC) on the basis of light microscopic, immunocytochemical, ultrastructural and immunoelectron microscopic investigations, were evaluated. The tumors showed a wide spectrum of histologic features that could be referred to three basic patterns: 1) a carcinoid-like pattern; 2) an organoid pattern characterized by palisading cells at the edge of cellular areas, and 3) a prevalent adenocarcinoma-like pattern. The second pattern was the most distinct even though it often mimicked the small cell/large cell subtype of small cell carcinoma (SCC) owing to its association with marked atypia and poor differentiation. All but one of the patients were males and smokers. The mean age was 58 years. Half of the tumors were centrally located including those showing the adenocarcinoma-like pattern. Disease-free and overall survival and type of tumor dissemination in four patients were similar to those of SCC. Five evaluable patients were alive and disease-free after a mean follow-up of 74 months. Two of these were initially diagnosed as SCC. We conclude that, because of its impact on prognosis, the diagnosis of WDNC appears to be relevant although other factors able to adversely affect the clinical course remain undefined.
Tumori
We examined the reactivity of two monoclonal antibodies (MAbs), MBrI and MBr8, on sections from n... more We examined the reactivity of two monoclonal antibodies (MAbs), MBrI and MBr8, on sections from normal breast tissues, benign lesions, primary infiltrating carcinomas and distant breast cancer metastases and compared the expression of the recognized antigens (CaMBr1 and CaMBr8 respectively) in these different normal and pathologic conditions. The expression of both antigens was found to significantly decrease in the neoplastic tissues, in comparison to normal breast tissue (34% vs 61% for MBr1 and 52% vs 86% for MBr8; p less than 0.001 for both). Atypical epithelial hyperplasia was found to be reactive on the same level (31%) of the tumors when tested with MBr1, whereas its reactivity was similar to that of normal tissues (78%) when MBr8 was used. Nonatypical epithelial hyperplasia showed the same reactivity with both MAbs (positive staining on about 50% of the cells). 65% of the "in situ" ductal carcinoma cases was MBr1-positive, whereas 50% was MBr8-positive; these values, when compared with the reactivity on normal glands, were significantly lower for MBr8, but not for MBr1. When testing sections from premenopausal women who had biopsies performed on different days during their menstrual cycle a correlation was found between the expression of CaMBr1 in normal breast epithelial cells and the stimulatory phase of the cycle. These findings suggest that both antigens are typical of normal breast and their expression could possibly decrease in pathologic tissues.
The Journal of Pathology
ABSTRACT
Anticancer research
Résumé/Abstract Carcinoma cell detachment is an important step in tumor progression and metastasi... more Résumé/Abstract Carcinoma cell detachment is an important step in tumor progression and metastasis. Episialin (EMA), if expressed throughout the entire cell surface, may inhibit cell-cell and cell-matrix adhesion. We investigated whether the cellular distribution of episialin ...
Histochemistry
A case of cirrhotic liver harbouring three atypical macroregenerative nodules and an hepatocellul... more A case of cirrhotic liver harbouring three atypical macroregenerative nodules and an hepatocellular carcinoma was immunocytochemically investigated for the expression of VLA-fl 1, VLA-a2 and VLA-~5 integrins and for different extracellular matrix (ECM) components (collagen I, collagen IV, laminin, fibronectin and tenascin). In addition, the proliferative activity within the nodules was evaluated, using the MIB 1 monoclonal antibody (MAb). The cirrhotic liver disclosed a continuous staining pattern of the ECM proteins investigated, as well as a "sinusoidal" immunostaining of VLA-fll, VLA-~2 and VLA-c~5. The macroregenerative nodules showed a discontinued immunoreactivity for ECM proteins while maintaining a VLA-fll sinusoidal immunostaining, coupled with intercellular immunostaining. VLA-~2 and VLA-a5 expression was lacking. The growth fraction was low in both the above pathological conditions. The hepatocellular carcinoma was devoid of any ECM immunostaining. VLA-fll immunoreactivity exhibited a honeycomb pattern of staining, whereas VLA subunits were absent. MIB 1 expression was high, being present in 30% of neoplastic nuclei. A possible relationship between atypical macroregenerative nodules and hepatocellular carcinoma is discussed.
Haematologica
A granulocytic sarcoma with tropism for serosal surfaces and minimal skin and bone marrow involve... more A granulocytic sarcoma with tropism for serosal surfaces and minimal skin and bone marrow involvement is reported. Serosal disease consisted both of a'superficial component'and a'tumor forming'counterpart. Chromosome analysis showed hypotriploid karyotype. Bone marrow involvement pattern and adhesion molecule immunoreactivity (IR) of neoplastic cells are discussed.
European Urology Supplements, 2015
The Journal of Urology, 2015
Tumori, Jan 30, 1992
The clinico-pathologic characteristics of 10 resected pulmonary tumors, which proved to be well-d... more The clinico-pathologic characteristics of 10 resected pulmonary tumors, which proved to be well-differentiated neuroendocrine carcinomas (WDNC) on the basis of light microscopic, immunocytochemical, ultrastructural and immunoelectron microscopic investigations, were evaluated. The tumors showed a wide spectrum of histologic features that could be referred to three basic patterns: 1) a carcinoid-like pattern; 2) an organoid pattern characterized by palisading cells at the edge of cellular areas, and 3) a prevalent adenocarcinoma-like pattern. The second pattern was the most distinct even though it often mimicked the small cell/large cell subtype of small cell carcinoma (SCC) owing to its association with marked atypia and poor differentiation. All but one of the patients were males and smokers. The mean age was 58 years. Half of the tumors were centrally located including those showing the adenocarcinoma-like pattern. Disease-free and overall survival and type of tumor dissemination ...
Journal of medical biography, 2014
Andrea Pasta was an eclectic visionary light years ahead of his time. He made numerous contributi... more Andrea Pasta was an eclectic visionary light years ahead of his time. He made numerous contributions to the field of medicine, some recognized by his contemporaries and others so visionary that they are being applied only in modern times. His contributions spanned the disciplines of psychology, gynaecology, haematology, infectious diseases and the doctor-patient relationship. Well known among his contemporaries, he combined a passion for clinical medicine and a keen interest in history and art with a strict research methodology and an approach to caring for patients as human beings. By studying his life and works, we can better understand the magnitude and significance of his innovative method and its applicability in modern times and also the significance of his many contributions.
Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology, 2010
To the Editors: Cell collection from serous effusions is a widely adopted practice in order to re... more To the Editors: Cell collection from serous effusions is a widely adopted practice in order to reach a diagnosis of possible diffusion of ascitogen tumors—ovarian carcinoma, gastric carcinoma, pancreatic carcinoma, liver carcinoma, etc., as well as pleural fluid in the case ...
Pathologica, 2008
We describe two cases of association between an ovarian granulosa cell tumour and a cystic mature... more We describe two cases of association between an ovarian granulosa cell tumour and a cystic mature teratoma and an epidermoid cyst. These are very rare combinations that might lead to incorrect diagnostic interpretation, since the simultaneous presence, on routine gross and microscopic examination, of a cystic squamous lesion, intimately connected with (case 1), or contralateral to (case 2) a solid, histologically trabecular or microfollicular tumour, might suggest a diagnosis of a carcinoid tumour in a mature cystic teratoma. The differential diagnostic problems and a review of the literature are presented.
Journal of gastrointestinal and liver diseases : JGLD, 2007
Pathology, research and practice, 2003
The histological patterns of anti-androgen-treated prostate adenocarcinoma mimic high grade tumor... more The histological patterns of anti-androgen-treated prostate adenocarcinoma mimic high grade tumors classified according to the widely used Gleason scoring system. However, the biological characteristics of anti-androgen treated carcinoma are largely unknown. E-cadherin, alpha-catenin, and beta-catenin adhesion molecules are down-regulated in pharmacologically untreated high grade prostate carcinoma. In this study, we used immunohistochemical techniques to investigate their expression in twenty acinar adenocarcinomas after anti-androgen therapy in prostatectomy specimens. After adrogen ablation therapy, expression of all these adhesion molecules was higher than that of pretreatment biopsies of the same patient group and high grade matched untreated controls. These results emphasize the inaccuracy of the Gleason score for anti-androgen-treated prostate adenocarcinoma and the more differentiated phenotype of prostate adenocarcinoma after anti-hormonal therapy.
International journal of surgical pathology, 2011
Nest-like structures are the cardinal lesion of different benign and malignant bladder entities. ... more Nest-like structures are the cardinal lesion of different benign and malignant bladder entities. These entities emphasize the biological relevance of the subtle morphological nest plasticity. The authors present 25 benign and malignant neoplasms, sharing the presence of florid nest-like growth and the scarcity or absence of superficial papillary components. Differential diagnostic clues are discussed.
Human Pathology, 1998
The differential expression of laminin receptors has been shown to modulate the invasive capabili... more The differential expression of laminin receptors has been shown to modulate the invasive capability of malignant cells. We have investigated the reactivity of human pulmonary squamous carcinomas (SSC, n = 20) and adenocarcinomas (ADC, n = 20) with monoclonal antibodies to the cytoplasmic and extracellular domains of the integrin subunits alpha3 and alpha6. Integrins containing these subunits are laminin receptors. Monoclonal antibodies to beta1 and beta4 subunits, the beta1C splice variant of beta1, as well as to Ki-67, were also used. Reverse transcription polymerase chain reaction (PCR) single-strand conformational polymorphism analysis was done to detect possible mutations in the cytodomains. All carcinomas expressed alpha3 extensively; alpha3 expression predominated (40 of 40) over alpha6 (25 of 40). In all alpha6-positive carcinomas, alpha6A was expressed, whereas alpha6B was weakly expressed only in some of them. No mutations of the intracytoplasmic domain A of alpha3 and of the A or B intracytoplasmic domains of alpha6 were shown. Notably, in normal bronchial epithelium, alpha6 colocalized with beta4, whereas in the tumors, alpha6A frequently overlapped with beta1 in a circumferential pattern; alpha6beta1 coexpression was also shown by coprecipitation experiments. Strong and extensive beta4 reactions were invariably polarized at the cell/stroma interface in SCC and ADC. An inverse correlation was found between the expression of beta1C and Ki-67. The prevalence of alpha6A in pulmonary SCC and ADC is in contrast with previous results in colonic ADC in which alpha6B prevails, and alpha6 predominates over alpha3. The absence of mutations of the cytodomains suggests that the integrin subunits of these carcinomas are potentially active. Predominance of alpha3 over alpha6 and of alpha6A over alpha6B may contribute to explain the aggressive and metastatic behavior of lung carcinomas.
Human Pathology, 2000
... Lack of CD 29 I Integrin) and CD 54 (ICAM-I) Adhesion Molecules in Intravascular Lymphomatosi... more ... Lack of CD 29 I Integrin) and CD 54 (ICAM-I) Adhesion Molecules in Intravascular Lymphomatosis MAURILIO PONZONI, MD, GIANLUIGI ARRIGONI, MD, VICTOR E ... N EnglJ Med 314:943-948, 1986 5. Sleater JR Segal GH, Scott MD, et al: Intravascular (Angio-tropic) large cell ...
Human Pathology, 1998
UK PubMed Central (UKPMC) is an archive of life sciences journal literature.
BioMed Research International, 2015
Morphologic criteria illustrated in WHO guidelines are the most significant prognostic factor in ... more Morphologic criteria illustrated in WHO guidelines are the most significant prognostic factor in human gliomas, but novel biomarkers are needed to identify patients with a poorer outcome. The present study examined the expression of the oncofetal protein IMP3 in a series of 135 patients affected by high-grade (grade III and IV) gliomas, correlating the results with proliferative activity, molecular parameters, and clinical and follow-up data. Overall, IMP3 expression was higher in glioblastomas (68%) than in grade III tumors (20%, < 0.0001), and IMP3-positive high-grade gliomas showed a shorter overall and disease-free survival than negative ones ( = 0.0002 and = 0.006, resp.). IMP3 expression was significantly associated with the absence of mutations of IDH1 gene ( = 0.0001) and with the unmethylated phenotype of MGMT in high-grade gliomas ( = 0.004). High Ki67 levels were correlated with better prognosis in glioblastomas but IMP3 expression was not correlated with the proliferation index. These findings confirm the role of IMP3 as a marker of poor outcome, also in consideration of its association with IDH1 wild-type phenotype and MGMT unmethylated status. The data suggest that IMP3 staining could identify a subgroup of patients with poor prognosis and at risk of recurrence in high-grade gliomas.
Tumori
In order to study the possible relationship between antigenic phenotype and tumor progression, 63... more In order to study the possible relationship between antigenic phenotype and tumor progression, 63 small cell lung carcinomas (SCLC) biopsies derived from primary or metastatic tumors were tested by immunofluorescence and immunoperoxidase techniques with an anti-carcinoma monoclonal antibody designated MBr1. Primary tumors were found to be less reactive with MBr1 than the local and distant metastatic lesions (57% versus 75% and 89% positivity respectively). A life table analysis on the tested cases indicated an inverse association between the expression of the marker recognized by the MBr1 MAb (CaMBr1) and overall survival (p less than 0.01): patients with MBr1-positive tumors showed a shorter survival time in comparison to patients whose tumors did not express the marker. The same correlation between survival and CaMBr1 expression was found even when only the 31 cases of early stage disease patients were considered (p less than 0.05). Different tumor aggressiveness or resistance to therapy of MBr1-positive tumors could be responsible for the shorter survival time of the patients.
Tumori
The clinico-pathologic characteristics of 10 resected pulmonary tumors, which proved to be well-d... more The clinico-pathologic characteristics of 10 resected pulmonary tumors, which proved to be well-differentiated neuroendocrine carcinomas (WDNC) on the basis of light microscopic, immunocytochemical, ultrastructural and immunoelectron microscopic investigations, were evaluated. The tumors showed a wide spectrum of histologic features that could be referred to three basic patterns: 1) a carcinoid-like pattern; 2) an organoid pattern characterized by palisading cells at the edge of cellular areas, and 3) a prevalent adenocarcinoma-like pattern. The second pattern was the most distinct even though it often mimicked the small cell/large cell subtype of small cell carcinoma (SCC) owing to its association with marked atypia and poor differentiation. All but one of the patients were males and smokers. The mean age was 58 years. Half of the tumors were centrally located including those showing the adenocarcinoma-like pattern. Disease-free and overall survival and type of tumor dissemination in four patients were similar to those of SCC. Five evaluable patients were alive and disease-free after a mean follow-up of 74 months. Two of these were initially diagnosed as SCC. We conclude that, because of its impact on prognosis, the diagnosis of WDNC appears to be relevant although other factors able to adversely affect the clinical course remain undefined.
Tumori
We examined the reactivity of two monoclonal antibodies (MAbs), MBrI and MBr8, on sections from n... more We examined the reactivity of two monoclonal antibodies (MAbs), MBrI and MBr8, on sections from normal breast tissues, benign lesions, primary infiltrating carcinomas and distant breast cancer metastases and compared the expression of the recognized antigens (CaMBr1 and CaMBr8 respectively) in these different normal and pathologic conditions. The expression of both antigens was found to significantly decrease in the neoplastic tissues, in comparison to normal breast tissue (34% vs 61% for MBr1 and 52% vs 86% for MBr8; p less than 0.001 for both). Atypical epithelial hyperplasia was found to be reactive on the same level (31%) of the tumors when tested with MBr1, whereas its reactivity was similar to that of normal tissues (78%) when MBr8 was used. Nonatypical epithelial hyperplasia showed the same reactivity with both MAbs (positive staining on about 50% of the cells). 65% of the "in situ" ductal carcinoma cases was MBr1-positive, whereas 50% was MBr8-positive; these values, when compared with the reactivity on normal glands, were significantly lower for MBr8, but not for MBr1. When testing sections from premenopausal women who had biopsies performed on different days during their menstrual cycle a correlation was found between the expression of CaMBr1 in normal breast epithelial cells and the stimulatory phase of the cycle. These findings suggest that both antigens are typical of normal breast and their expression could possibly decrease in pathologic tissues.
The Journal of Pathology
ABSTRACT
Anticancer research
Résumé/Abstract Carcinoma cell detachment is an important step in tumor progression and metastasi... more Résumé/Abstract Carcinoma cell detachment is an important step in tumor progression and metastasis. Episialin (EMA), if expressed throughout the entire cell surface, may inhibit cell-cell and cell-matrix adhesion. We investigated whether the cellular distribution of episialin ...
Histochemistry
A case of cirrhotic liver harbouring three atypical macroregenerative nodules and an hepatocellul... more A case of cirrhotic liver harbouring three atypical macroregenerative nodules and an hepatocellular carcinoma was immunocytochemically investigated for the expression of VLA-fl 1, VLA-a2 and VLA-~5 integrins and for different extracellular matrix (ECM) components (collagen I, collagen IV, laminin, fibronectin and tenascin). In addition, the proliferative activity within the nodules was evaluated, using the MIB 1 monoclonal antibody (MAb). The cirrhotic liver disclosed a continuous staining pattern of the ECM proteins investigated, as well as a "sinusoidal" immunostaining of VLA-fll, VLA-~2 and VLA-c~5. The macroregenerative nodules showed a discontinued immunoreactivity for ECM proteins while maintaining a VLA-fll sinusoidal immunostaining, coupled with intercellular immunostaining. VLA-~2 and VLA-a5 expression was lacking. The growth fraction was low in both the above pathological conditions. The hepatocellular carcinoma was devoid of any ECM immunostaining. VLA-fll immunoreactivity exhibited a honeycomb pattern of staining, whereas VLA subunits were absent. MIB 1 expression was high, being present in 30% of neoplastic nuclei. A possible relationship between atypical macroregenerative nodules and hepatocellular carcinoma is discussed.
Haematologica
A granulocytic sarcoma with tropism for serosal surfaces and minimal skin and bone marrow involve... more A granulocytic sarcoma with tropism for serosal surfaces and minimal skin and bone marrow involvement is reported. Serosal disease consisted both of a&amp;amp;#x27;superficial component&amp;amp;#x27;and a&amp;amp;#x27;tumor forming&amp;amp;#x27;counterpart. Chromosome analysis showed hypotriploid karyotype. Bone marrow involvement pattern and adhesion molecule immunoreactivity (IR) of neoplastic cells are discussed.
European Urology Supplements, 2015
The Journal of Urology, 2015
Tumori, Jan 30, 1992
The clinico-pathologic characteristics of 10 resected pulmonary tumors, which proved to be well-d... more The clinico-pathologic characteristics of 10 resected pulmonary tumors, which proved to be well-differentiated neuroendocrine carcinomas (WDNC) on the basis of light microscopic, immunocytochemical, ultrastructural and immunoelectron microscopic investigations, were evaluated. The tumors showed a wide spectrum of histologic features that could be referred to three basic patterns: 1) a carcinoid-like pattern; 2) an organoid pattern characterized by palisading cells at the edge of cellular areas, and 3) a prevalent adenocarcinoma-like pattern. The second pattern was the most distinct even though it often mimicked the small cell/large cell subtype of small cell carcinoma (SCC) owing to its association with marked atypia and poor differentiation. All but one of the patients were males and smokers. The mean age was 58 years. Half of the tumors were centrally located including those showing the adenocarcinoma-like pattern. Disease-free and overall survival and type of tumor dissemination ...
Journal of medical biography, 2014
Andrea Pasta was an eclectic visionary light years ahead of his time. He made numerous contributi... more Andrea Pasta was an eclectic visionary light years ahead of his time. He made numerous contributions to the field of medicine, some recognized by his contemporaries and others so visionary that they are being applied only in modern times. His contributions spanned the disciplines of psychology, gynaecology, haematology, infectious diseases and the doctor-patient relationship. Well known among his contemporaries, he combined a passion for clinical medicine and a keen interest in history and art with a strict research methodology and an approach to caring for patients as human beings. By studying his life and works, we can better understand the magnitude and significance of his innovative method and its applicability in modern times and also the significance of his many contributions.
Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology, 2010
To the Editors: Cell collection from serous effusions is a widely adopted practice in order to re... more To the Editors: Cell collection from serous effusions is a widely adopted practice in order to reach a diagnosis of possible diffusion of ascitogen tumors—ovarian carcinoma, gastric carcinoma, pancreatic carcinoma, liver carcinoma, etc., as well as pleural fluid in the case ...
Pathologica, 2008
We describe two cases of association between an ovarian granulosa cell tumour and a cystic mature... more We describe two cases of association between an ovarian granulosa cell tumour and a cystic mature teratoma and an epidermoid cyst. These are very rare combinations that might lead to incorrect diagnostic interpretation, since the simultaneous presence, on routine gross and microscopic examination, of a cystic squamous lesion, intimately connected with (case 1), or contralateral to (case 2) a solid, histologically trabecular or microfollicular tumour, might suggest a diagnosis of a carcinoid tumour in a mature cystic teratoma. The differential diagnostic problems and a review of the literature are presented.
Journal of gastrointestinal and liver diseases : JGLD, 2007
Pathology, research and practice, 2003
The histological patterns of anti-androgen-treated prostate adenocarcinoma mimic high grade tumor... more The histological patterns of anti-androgen-treated prostate adenocarcinoma mimic high grade tumors classified according to the widely used Gleason scoring system. However, the biological characteristics of anti-androgen treated carcinoma are largely unknown. E-cadherin, alpha-catenin, and beta-catenin adhesion molecules are down-regulated in pharmacologically untreated high grade prostate carcinoma. In this study, we used immunohistochemical techniques to investigate their expression in twenty acinar adenocarcinomas after anti-androgen therapy in prostatectomy specimens. After adrogen ablation therapy, expression of all these adhesion molecules was higher than that of pretreatment biopsies of the same patient group and high grade matched untreated controls. These results emphasize the inaccuracy of the Gleason score for anti-androgen-treated prostate adenocarcinoma and the more differentiated phenotype of prostate adenocarcinoma after anti-hormonal therapy.
International journal of surgical pathology, 2011
Nest-like structures are the cardinal lesion of different benign and malignant bladder entities. ... more Nest-like structures are the cardinal lesion of different benign and malignant bladder entities. These entities emphasize the biological relevance of the subtle morphological nest plasticity. The authors present 25 benign and malignant neoplasms, sharing the presence of florid nest-like growth and the scarcity or absence of superficial papillary components. Differential diagnostic clues are discussed.
Human Pathology, 1998
The differential expression of laminin receptors has been shown to modulate the invasive capabili... more The differential expression of laminin receptors has been shown to modulate the invasive capability of malignant cells. We have investigated the reactivity of human pulmonary squamous carcinomas (SSC, n = 20) and adenocarcinomas (ADC, n = 20) with monoclonal antibodies to the cytoplasmic and extracellular domains of the integrin subunits alpha3 and alpha6. Integrins containing these subunits are laminin receptors. Monoclonal antibodies to beta1 and beta4 subunits, the beta1C splice variant of beta1, as well as to Ki-67, were also used. Reverse transcription polymerase chain reaction (PCR) single-strand conformational polymorphism analysis was done to detect possible mutations in the cytodomains. All carcinomas expressed alpha3 extensively; alpha3 expression predominated (40 of 40) over alpha6 (25 of 40). In all alpha6-positive carcinomas, alpha6A was expressed, whereas alpha6B was weakly expressed only in some of them. No mutations of the intracytoplasmic domain A of alpha3 and of the A or B intracytoplasmic domains of alpha6 were shown. Notably, in normal bronchial epithelium, alpha6 colocalized with beta4, whereas in the tumors, alpha6A frequently overlapped with beta1 in a circumferential pattern; alpha6beta1 coexpression was also shown by coprecipitation experiments. Strong and extensive beta4 reactions were invariably polarized at the cell/stroma interface in SCC and ADC. An inverse correlation was found between the expression of beta1C and Ki-67. The prevalence of alpha6A in pulmonary SCC and ADC is in contrast with previous results in colonic ADC in which alpha6B prevails, and alpha6 predominates over alpha3. The absence of mutations of the cytodomains suggests that the integrin subunits of these carcinomas are potentially active. Predominance of alpha3 over alpha6 and of alpha6A over alpha6B may contribute to explain the aggressive and metastatic behavior of lung carcinomas.
Human Pathology, 2000
... Lack of CD 29 I Integrin) and CD 54 (ICAM-I) Adhesion Molecules in Intravascular Lymphomatosi... more ... Lack of CD 29 I Integrin) and CD 54 (ICAM-I) Adhesion Molecules in Intravascular Lymphomatosis MAURILIO PONZONI, MD, GIANLUIGI ARRIGONI, MD, VICTOR E ... N EnglJ Med 314:943-948, 1986 5. Sleater JR Segal GH, Scott MD, et al: Intravascular (Angio-tropic) large cell ...
Human Pathology, 1998
UK PubMed Central (UKPMC) is an archive of life sciences journal literature.
BioMed Research International, 2015
Morphologic criteria illustrated in WHO guidelines are the most significant prognostic factor in ... more Morphologic criteria illustrated in WHO guidelines are the most significant prognostic factor in human gliomas, but novel biomarkers are needed to identify patients with a poorer outcome. The present study examined the expression of the oncofetal protein IMP3 in a series of 135 patients affected by high-grade (grade III and IV) gliomas, correlating the results with proliferative activity, molecular parameters, and clinical and follow-up data. Overall, IMP3 expression was higher in glioblastomas (68%) than in grade III tumors (20%, < 0.0001), and IMP3-positive high-grade gliomas showed a shorter overall and disease-free survival than negative ones ( = 0.0002 and = 0.006, resp.). IMP3 expression was significantly associated with the absence of mutations of IDH1 gene ( = 0.0001) and with the unmethylated phenotype of MGMT in high-grade gliomas ( = 0.004). High Ki67 levels were correlated with better prognosis in glioblastomas but IMP3 expression was not correlated with the proliferation index. These findings confirm the role of IMP3 as a marker of poor outcome, also in consideration of its association with IDH1 wild-type phenotype and MGMT unmethylated status. The data suggest that IMP3 staining could identify a subgroup of patients with poor prognosis and at risk of recurrence in high-grade gliomas.