Carlos Gemin - Academia.edu (original) (raw)

Papers by Carlos Gemin

Asian Pacific Journal of Tropical Medicine, 2014

Some members of this genus are used as food spices to flavor meats and as a flavoring agents in p... more Some members of this genus are used as food spices to flavor meats and as a flavoring agents in perfumery and cosmetics. Additionally, they have pharmacologically effect and used in folk medicine; they were used to treat colds, wounds, insomnia and skin infections as well as headache, cerebral ischaemia and memory disorders. Sages have been used as antihydrotic, spasmolytic, anticholinesterase, antiseptic, insecticide, antifungal, antioxidant, anti-inflammatory and also in the treatment of mental and nervous conditions. Moreover, cardioactive and antibacterial terpenoids were isolated from the extracts of this genus. Salvia is a rich source of phytochemicals including phenolic acids, polyphenols, flavonoid glycosides, a n t h o c y a n i n s , s e s q u i t e r p e n o i d s , d i t e r p e n o i d s , sesterterpenes and triterpenes [1,2,4,8-12]. Antioxidants have great importance because they can reduce oxidative stress which could cause damage to biological molecules. Antioxidant compounds play a crucial role in the treatment of various deseases related to degenerative disorders, namely, cardiovascular and brain diseases, arthritis, diabetes, cancer and immune system decline, by acting as free radical scavengers, and thus decreasing the extent of oxidative damage. Furthermore, studies about antioxidant substances in foods and medicinal natural sources have attracted increased interest in the recent decades. In addition, the use of plant materials in lipids and lipid-containing foods is important because the plant potentials of decreasing rancidity, delaying the formation of toxic oxidation products, maintaining nutritional quality and increasing the shelf life of food products. Hence, evaluation of radical scavenging properties and antioxidant activity are of commercial interest to the pharmaceutical and food industries as a source of natural antioxidants [7,9,10,12-14]. Microbial actions of essential oils are also one of the most extensively studied features of botanical medicine and various aromatic plant species were being investigated for their pharmacological properties [6,11,15,16]. To the best of our knowledge, there has been no published detailed information about the composition, antioxidant and antibacterial activities of the essential oil of aerial part of Salvia sclareoides (S. sclareoides). Therefore, the objectives of the present study were to identify chemical composition as well as assess the antioxidant and antibacterial properties of the essential oil of the aerial part of S. sclareoides collected from the western of Iran using gas chromatography combined with mass spectrometry (GC-MS) and flame ionization detector.

Page 1. UNIVERSIDADE FEDERAL DO PARANÁ PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS CARLOS... more Page 1. UNIVERSIDADE FEDERAL DO PARANÁ PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS CARLOS ALBERTO BRUEL GEMIN ESTUDO FITOQUÍMICO E AVALIAÇÃO DE ATIVIDADES BIOLÓGICAS ...

Nutrition, Jan 6, 2014

Background: In the present study, hexane: methanol (50:50) leaf extract of Marisela minuta has be... more Background: In the present study, hexane: methanol (50:50) leaf extract of Marisela minuta has been evaluated for its chemical composition, antioxidant effect and the antimicrobial mechanism of action against food borne pathogenic bacteria. Results: The phytochemical evaluation of extract by GC/MS revealed the major abundance of benzoic acid-4-ethoxyethyl ester (43.39%) and farnesol acetate (18.42%). The extract exhibited potential antioxidant and free radical scavenging properties with promising antibacterial activities against the test pathogens with Pseudomonas aeruginosa being the most susceptible with maximum inhibition zone (17 mm) and IC 50 value of 125 µg, respectively. The significant (p < 0.05) increase in intracellular super oxide dismutase (SOD), protein leakage, extracellular alkaline phosphatase and lactate dehydrogenase in treated test pathogens suggested an increase in oxidative stress reveling the mechanism of action of phytochemicals. Scanning electron microscopy analysis of treated pathogens also showed swollen and distorted cells. The bioactive molecules in the extract were efficiently docked with virulent enzymes and farnesol acetate showed best energy value of − 5.19 and − 4.27 kcal/mol towards Topoisomerase IV and SHV-2 respectively. Benzoic acid-4-ethoxyethyl ester showed best binding against TEM-72 with low binding energy value of − 4.35 kcal/mol. Conclusion: Due to its antioxidant and antibacterial properties, the leaf extract of M. minuta may act as promising natural additives to prevent food spoilage bacteria.

International Journal of Phytomedicine

Journal of Analytical …, 2012

Trichilia catigua is a Brazilian native species known as "catuaba". This study investigated the c... more Trichilia catigua is a Brazilian native species known as "catuaba". This study investigated the chromatographic features hydroalcoholic extracts that were obtained by different techniques, from barks and leaves. The chromatographic analysis has demonstrated the presence of chlorogenic acid, epicathechin and cathechin, as well as the presence of β-sitosterol and stigmasterol in both analyzed plant parts. The best results were those when it was used turbo-extraction, extraction using Soxhlet apparatus and ethanol 50%.

The aim of this study was to evaluate the flavonoid content of an ethanolic leaf extract from the... more The aim of this study was to evaluate the flavonoid content of an ethanolic leaf extract from the medicinal plant Rourea induta Planch. (RIEE) and to investigate its hepatoprotective potential and in vivo antioxidant effects. Methods: Using samples from carbon tetrachloride-treated Wistar female rats treated orally with or without RIEE, we evaluated the aspartate aminotransferase, alanine aminotransferase, and total bilirubin levels in plasma; the levels of the hepatic oxidative stress markers catalase, superoxide dismutase, glutathione peroxidase, and reduced glutathione in liver homogenates; and the thiobarbituric acid reactive substance levels. A histopathology study was performed. A quantitative analysis of the RIEE extract was performed using high-performance liquid chromatography to evaluate its flavonoid content. Results: Oral administration of RIEE significantly reduced carbon tetrachloride-induced elevations in the levels of plasma markers of hepatic damage and lipid peroxidation. It also rescued histopathologic alterations observed in the liver and levels of oxidative stress markers. Conclusions: RIEE exhibits antioxidant and hepatoprotective activities in vivo, which may be attributable to its flavonoids composition [hyperin (2), quercetin-3-O-b-xyloside (4), quercetin-3-O-a-arabinofuranoside (5), and quercetin (6)].

Asian Pacific Journal of Tropical Medicine, 2014

Some members of this genus are used as food spices to flavor meats and as a flavoring agents in p... more Some members of this genus are used as food spices to flavor meats and as a flavoring agents in perfumery and cosmetics. Additionally, they have pharmacologically effect and used in folk medicine; they were used to treat colds, wounds, insomnia and skin infections as well as headache, cerebral ischaemia and memory disorders. Sages have been used as antihydrotic, spasmolytic, anticholinesterase, antiseptic, insecticide, antifungal, antioxidant, anti-inflammatory and also in the treatment of mental and nervous conditions. Moreover, cardioactive and antibacterial terpenoids were isolated from the extracts of this genus. Salvia is a rich source of phytochemicals including phenolic acids, polyphenols, flavonoid glycosides, a n t h o c y a n i n s , s e s q u i t e r p e n o i d s , d i t e r p e n o i d s , sesterterpenes and triterpenes [1,2,4,8-12]. Antioxidants have great importance because they can reduce oxidative stress which could cause damage to biological molecules. Antioxidant compounds play a crucial role in the treatment of various deseases related to degenerative disorders, namely, cardiovascular and brain diseases, arthritis, diabetes, cancer and immune system decline, by acting as free radical scavengers, and thus decreasing the extent of oxidative damage. Furthermore, studies about antioxidant substances in foods and medicinal natural sources have attracted increased interest in the recent decades. In addition, the use of plant materials in lipids and lipid-containing foods is important because the plant potentials of decreasing rancidity, delaying the formation of toxic oxidation products, maintaining nutritional quality and increasing the shelf life of food products. Hence, evaluation of radical scavenging properties and antioxidant activity are of commercial interest to the pharmaceutical and food industries as a source of natural antioxidants [7,9,10,12-14]. Microbial actions of essential oils are also one of the most extensively studied features of botanical medicine and various aromatic plant species were being investigated for their pharmacological properties [6,11,15,16]. To the best of our knowledge, there has been no published detailed information about the composition, antioxidant and antibacterial activities of the essential oil of aerial part of Salvia sclareoides (S. sclareoides). Therefore, the objectives of the present study were to identify chemical composition as well as assess the antioxidant and antibacterial properties of the essential oil of the aerial part of S. sclareoides collected from the western of Iran using gas chromatography combined with mass spectrometry (GC-MS) and flame ionization detector.

Page 1. UNIVERSIDADE FEDERAL DO PARANÁ PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS CARLOS... more Page 1. UNIVERSIDADE FEDERAL DO PARANÁ PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS CARLOS ALBERTO BRUEL GEMIN ESTUDO FITOQUÍMICO E AVALIAÇÃO DE ATIVIDADES BIOLÓGICAS ...

Nutrition, Jan 6, 2014

Background: In the present study, hexane: methanol (50:50) leaf extract of Marisela minuta has be... more Background: In the present study, hexane: methanol (50:50) leaf extract of Marisela minuta has been evaluated for its chemical composition, antioxidant effect and the antimicrobial mechanism of action against food borne pathogenic bacteria. Results: The phytochemical evaluation of extract by GC/MS revealed the major abundance of benzoic acid-4-ethoxyethyl ester (43.39%) and farnesol acetate (18.42%). The extract exhibited potential antioxidant and free radical scavenging properties with promising antibacterial activities against the test pathogens with Pseudomonas aeruginosa being the most susceptible with maximum inhibition zone (17 mm) and IC 50 value of 125 µg, respectively. The significant (p < 0.05) increase in intracellular super oxide dismutase (SOD), protein leakage, extracellular alkaline phosphatase and lactate dehydrogenase in treated test pathogens suggested an increase in oxidative stress reveling the mechanism of action of phytochemicals. Scanning electron microscopy analysis of treated pathogens also showed swollen and distorted cells. The bioactive molecules in the extract were efficiently docked with virulent enzymes and farnesol acetate showed best energy value of − 5.19 and − 4.27 kcal/mol towards Topoisomerase IV and SHV-2 respectively. Benzoic acid-4-ethoxyethyl ester showed best binding against TEM-72 with low binding energy value of − 4.35 kcal/mol. Conclusion: Due to its antioxidant and antibacterial properties, the leaf extract of M. minuta may act as promising natural additives to prevent food spoilage bacteria.

International Journal of Phytomedicine

Journal of Analytical …, 2012

Trichilia catigua is a Brazilian native species known as "catuaba". This study investigated the c... more Trichilia catigua is a Brazilian native species known as "catuaba". This study investigated the chromatographic features hydroalcoholic extracts that were obtained by different techniques, from barks and leaves. The chromatographic analysis has demonstrated the presence of chlorogenic acid, epicathechin and cathechin, as well as the presence of β-sitosterol and stigmasterol in both analyzed plant parts. The best results were those when it was used turbo-extraction, extraction using Soxhlet apparatus and ethanol 50%.

The aim of this study was to evaluate the flavonoid content of an ethanolic leaf extract from the... more The aim of this study was to evaluate the flavonoid content of an ethanolic leaf extract from the medicinal plant Rourea induta Planch. (RIEE) and to investigate its hepatoprotective potential and in vivo antioxidant effects. Methods: Using samples from carbon tetrachloride-treated Wistar female rats treated orally with or without RIEE, we evaluated the aspartate aminotransferase, alanine aminotransferase, and total bilirubin levels in plasma; the levels of the hepatic oxidative stress markers catalase, superoxide dismutase, glutathione peroxidase, and reduced glutathione in liver homogenates; and the thiobarbituric acid reactive substance levels. A histopathology study was performed. A quantitative analysis of the RIEE extract was performed using high-performance liquid chromatography to evaluate its flavonoid content. Results: Oral administration of RIEE significantly reduced carbon tetrachloride-induced elevations in the levels of plasma markers of hepatic damage and lipid peroxidation. It also rescued histopathologic alterations observed in the liver and levels of oxidative stress markers. Conclusions: RIEE exhibits antioxidant and hepatoprotective activities in vivo, which may be attributable to its flavonoids composition [hyperin (2), quercetin-3-O-b-xyloside (4), quercetin-3-O-a-arabinofuranoside (5), and quercetin (6)].