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Papers by Carlos Meneses
Molecular Plant-Microbe Interactions, 2011
Gluconacetobacter diazotrophicus PAL5 (PAL5) revealed the presence of a gum gene cluster. In this... more Gluconacetobacter diazotrophicus PAL5 (PAL5) revealed the presence of a gum gene cluster. In this study, the gumD gene homologue, which is predicted to be responsible for the first step in exopolysaccharide (EPS) production, was insertionally inactivated and the resultant mutant (MGD) was functionally studied. The mutant MGD presented normal growth and nitrogen (N 2 ) fixation levels but did not produce EPS when grown on different carbon sources. MGD presented altered colony morphology on soft agar plates (0.3% agar) and was defective in biofilm formation on glass wool. Most interestingly, MGD was defective in rice root surface attachment and in root surface and endophytic colonization. Genetic complementation reverted all mutant phenotypes. Also, the addition of EPS purified from culture supernatants of the wild-type strain PAL5 to the mutant MGD was effective in partially restoring wild-type biofilm formation and plant colonization. These data provide strong evidence that the PAL5 gumD gene is involved in EPS biosynthesis and that EPS biosynthesis is required for biofilm formation and plant colonization. To our knowledge, this is the first report of a role of EPS in the endophytic colonization of graminaceous plants by a nitrogen-fixing bacterium.
IEEE Transactions on Industry Applications, 2000
The subject of modern plant design as related to the fully automated Pertigalete plant is discuss... more The subject of modern plant design as related to the fully automated Pertigalete plant is discussed. The successive steps of the introduction of process automation in the light of plant integration are sketched. A general description of the system is presented. Some views are given on the influence of computer control on basic plant concept. The influence of automation on operating strategy, plant personnel, and equipment suppliers is also discussed.
BMC Genomics, 2009
Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in assoc... more Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins.
Para poder representar de manera adecuada los aspectos estáticos y dinámicos de un sistema acorde... more Para poder representar de manera adecuada los aspectos estáticos y dinámicos de un sistema acorde con las necesidades de modelización del mundo real, es importante incluir la expresividad temporal cuya especificación permita obtener un modelo funcionalmente equivalente al modelo conceptual del sistema estudiado.
Remote sensing and monitoring of vegetation and climate on time scales of the growing season and ... more Remote sensing and monitoring of vegetation and climate on time scales of the growing season and shorter can provide important information for agricultural and food security planning. Projects such as the United States Department of Agriculture's (USDA) CropExplorer and the well-known Famine Early Warning System Network (FEWSNET) are prominent examples of how to bring remotely sensed information to decision-makers in agriculture and food security. These systems have some shortcomings that limit their utility, especially by researchers that need remote sensing imagery before processing. Another shortcoming with these systems is that the original remote sensing data for the assessments is not widely available to a broad range of researchers in a given country. Remote sensing data is often difficult to acquire in countries that do not have the satellite systems which provide the data. How might some of these limitations be overcome? How could we facilitate increased use of remote s...
New remote sensing platforms and data programs have dramatically increased the availability of sa... more New remote sensing platforms and data programs have dramatically increased the availability of satellite image data for analysis of climate, agriculture, environment and society. Particularly important new sensor systems include the USA's MODIS system, Brazil and China's CBER platform, and India's IRS satellite. These and other systems have created considerable benefits to the international community of remote sensing analysts. Today, we have more data with greater options regarding spatial, radiometric and temporal resolution. While having these greater options is a positive development, substantial problems remain in acquiring and managing large data volumes. Data providers and consumers must support significant costs in copying remote sensing data to tapes and disks. Internet transfer of satellite imagery is only possible on broadband networks. Even then, download times can be considerable. Downloads may be interrupted if the Internet connections are unstable. How can...
Molecular Plant-Microbe Interactions, 2011
Gluconacetobacter diazotrophicus PAL5 (PAL5) revealed the presence of a gum gene cluster. In this... more Gluconacetobacter diazotrophicus PAL5 (PAL5) revealed the presence of a gum gene cluster. In this study, the gumD gene homologue, which is predicted to be responsible for the first step in exopolysaccharide (EPS) production, was insertionally inactivated and the resultant mutant (MGD) was functionally studied. The mutant MGD presented normal growth and nitrogen (N 2 ) fixation levels but did not produce EPS when grown on different carbon sources. MGD presented altered colony morphology on soft agar plates (0.3% agar) and was defective in biofilm formation on glass wool. Most interestingly, MGD was defective in rice root surface attachment and in root surface and endophytic colonization. Genetic complementation reverted all mutant phenotypes. Also, the addition of EPS purified from culture supernatants of the wild-type strain PAL5 to the mutant MGD was effective in partially restoring wild-type biofilm formation and plant colonization. These data provide strong evidence that the PAL5 gumD gene is involved in EPS biosynthesis and that EPS biosynthesis is required for biofilm formation and plant colonization. To our knowledge, this is the first report of a role of EPS in the endophytic colonization of graminaceous plants by a nitrogen-fixing bacterium.
IEEE Transactions on Industry Applications, 2000
The subject of modern plant design as related to the fully automated Pertigalete plant is discuss... more The subject of modern plant design as related to the fully automated Pertigalete plant is discussed. The successive steps of the introduction of process automation in the light of plant integration are sketched. A general description of the system is presented. Some views are given on the influence of computer control on basic plant concept. The influence of automation on operating strategy, plant personnel, and equipment suppliers is also discussed.
BMC Genomics, 2009
Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in assoc... more Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins.
Para poder representar de manera adecuada los aspectos estáticos y dinámicos de un sistema acorde... more Para poder representar de manera adecuada los aspectos estáticos y dinámicos de un sistema acorde con las necesidades de modelización del mundo real, es importante incluir la expresividad temporal cuya especificación permita obtener un modelo funcionalmente equivalente al modelo conceptual del sistema estudiado.
Remote sensing and monitoring of vegetation and climate on time scales of the growing season and ... more Remote sensing and monitoring of vegetation and climate on time scales of the growing season and shorter can provide important information for agricultural and food security planning. Projects such as the United States Department of Agriculture's (USDA) CropExplorer and the well-known Famine Early Warning System Network (FEWSNET) are prominent examples of how to bring remotely sensed information to decision-makers in agriculture and food security. These systems have some shortcomings that limit their utility, especially by researchers that need remote sensing imagery before processing. Another shortcoming with these systems is that the original remote sensing data for the assessments is not widely available to a broad range of researchers in a given country. Remote sensing data is often difficult to acquire in countries that do not have the satellite systems which provide the data. How might some of these limitations be overcome? How could we facilitate increased use of remote s...
New remote sensing platforms and data programs have dramatically increased the availability of sa... more New remote sensing platforms and data programs have dramatically increased the availability of satellite image data for analysis of climate, agriculture, environment and society. Particularly important new sensor systems include the USA's MODIS system, Brazil and China's CBER platform, and India's IRS satellite. These and other systems have created considerable benefits to the international community of remote sensing analysts. Today, we have more data with greater options regarding spatial, radiometric and temporal resolution. While having these greater options is a positive development, substantial problems remain in acquiring and managing large data volumes. Data providers and consumers must support significant costs in copying remote sensing data to tapes and disks. Internet transfer of satellite imagery is only possible on broadband networks. Even then, download times can be considerable. Downloads may be interrupted if the Internet connections are unstable. How can...