Carlos Palomino - Academia.edu (original) (raw)

Papers by Carlos Palomino

EDULEARN Proceedings

Lecturers of the Faculty of Sciences at the University of the Balearic Islands (Spain) have criti... more Lecturers of the Faculty of Sciences at the University of the Balearic Islands (Spain) have critically reviewed the first-year laboratory lectures of science for Biology, Biochemistry, Chemistry, Physics and engineering degrees. In all the studies, the experiments conducted in the laboratory help in the achievement of specific, generic and basic skills of each degree.

Resumen de la publicación de una participación en formato µVídeo en en el congreso Divulga NextGe... more Resumen de la publicación de una participación en formato µVídeo en en el congreso Divulga NextGen (1ª edición) que se celebrará online, de manera gratuita y en las redes sociales los días 26, 27 y 28 de mayo de 2021.

Sensors and Actuators B: Chemical, 2022

EDULEARN Proceedings, 2021

EDULEARN18 Proceedings, 2018

Journal of Cataract and Refractive Surgery, 2019

To evaluate the quality of vision after excimer laser (MEL 80) correction of presbyopia using mic... more To evaluate the quality of vision after excimer laser (MEL 80) correction of presbyopia using micromonovision and aspheric aberration patterns.

$Research paper thumbnail of Comparing surgically induced astigmatism calculated by means of simulated keratometry versus total corneal refractive power$

European journal of ophthalmology, 2018

To evaluate surgically induced astigmatism as computed by means of either simulated keratometry (... more To evaluate surgically induced astigmatism as computed by means of either simulated keratometry (K) or total corneal refractive power (TCRP) after temporal incisions. Prospective observational study including 36 right eyes undergoing cataract surgery. Astigmatism was measured preoperatively during the 3-month follow-up period using Pentacam. Surgically induced astigmatism was computed considering anterior corneal surface astigmatism at 3 mm with K and considering both corneal surfaces with TCRP from 1 to 8 mm (TCRP for 3 mm). The eyes under study were divided into two balanced groups: LOW with K astigmatism <0.90 D and HIGH with K astigmatism ≥0.90 D. Resulting surgically induced astigmatism values were compared across groups and measuring techniques by means of flattening, steepening, and torque analysis. Mean surgically induced astigmatism was higher in the HIGH group (0.31 D @ 102°) than in the LOW group (0.04 D @ 16°). The temporal incision resulted in a steepening in the HIG...

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 2006

Tuberculosis (TB) diagnostic laboratories in Latin America. Evaluation of thin-layer agar (TLA) c... more Tuberculosis (TB) diagnostic laboratories in Latin America. Evaluation of thin-layer agar (TLA) compared to Löwenstein-Jensen (LJ) culture for the diagnosis of TB. Phase II prospective study in six laboratories. Samples included sputum and extra-pulmonary specimens from patients with a clinical diagnosis of TB. Respiratory samples were decontaminated using NaOH/ NALC; all samples were centrifuged, stained with Ziehl-Neelsen for acid-fast bacilli (AFB), cultured on LJ and TLA and identified according to recommended procedures. Sensitivity and likelihood ratios (LR), growth detection time and contamination rate were calculated for both media. A total of 1118 clinical specimens were studied. Cultures detected Mycobacterium tuberculosis in all AFB-positive samples, whereas for AFB-negative specimens LJ detected 3.2% and TLA 4.4%. Sensitivity was 92.6% (95%CI 87.9-95.9) and 84.7% (95%CI 78.8-89.0) for TLA and LJ, respectively. Positive and negative LRs were similar. Contamination was 5.1...

Journal of Clinical Microbiology, 2008

For rapid and low-cost detection of multidrug-resistant (MDR) Mycobacterium tuberculosis , we app... more For rapid and low-cost detection of multidrug-resistant (MDR) Mycobacterium tuberculosis , we applied the nitrate reductase assay (NRA) using a liquid medium directly to sputum samples. A total of 179 sputum samples were analyzed by the NRA, and results were compared to those obtained by the indirect proportion method (IPM) as a standard reference. Out of 144 specimens for which comparable results were available, only one discrepant result was obtained: MDR by NRA but susceptible by the IPM. In total 56% of the results were obtained in 10 days by the NRA. NRA performed in liquid medium is rapid and inexpensive and can be easily implemented in low-income countries.

Journal of Antimicrobial Chemotherapy, 2008

The purpose of this study was to develop the nitrate reductase assay (NRA) for the rapid detectio... more The purpose of this study was to develop the nitrate reductase assay (NRA) for the rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide resistance as a marker of pyrazinamide resistance in Lö wenstein-Jensen (LJ) medium at neutral pH. Methods: We tested 68 M. tuberculosis isolates using nicotinamide at three different concentrations (1000, 500 and 250 mg/L) by the NRA in LJ medium and compared the results with those obtained with the BACTEC 460-TB or the BACTEC MGIT 960 as reference standard methods. Mutations in the pncA gene were detected by DNA sequencing of the pyrazinamide-resistant isolates. Results: Out of 34 M. tuberculosis pyrazinamide-resistant isolates, 31 were found to be resistant to 1000 and 500 mg/L nicotinamide giving sensitivity and specificity of 91% and 94%, respectively. At 250 mg/L nicotinamide, the sensitivity and specificity decreased to 91% and 71%, respectively. Results were obtained in an average of 10 days. Based on these results, a tentative breakpoint concentration of 500 mg/L nicotinamide was defined. DNA sequencing of the pncA gene detected mutations in 26 out of 34 M. tuberculosis isolates resistant to pyrazinamide. Conclusions: The NRA using nicotinamide to detect resistance to pyrazinamide in LJ medium is a rapid and accurate method that could be useful in limited-resource countries where the BACTEC 460-TB or the BACTEC MGIT 960 system is not available.

FEMS Microbiology Reviews, 2005

In contrast to the notorious pathogens Mycobacterium tuberculosis and M. leprae, the majority of ... more In contrast to the notorious pathogens Mycobacterium tuberculosis and M. leprae, the majority of the mycobacterial species described to date are generally not considered as obligate human pathogens. The natural reservoirs of these non-primary pathogenic mycobacteria include aquatic and terrestrial environments. Under certain circumstances, e.g., skin lesions, pulmonary or immune dysfunctions and chronic diseases, these environmental mycobacteria (EM) may cause disease. EM such as M. avium, M. kansasii, and M. xenopi have frequently been isolated from drinking water and hospital water distribution systems. Biofilm formation, amoeba-associated lifestyle, and resistance to chlorine have been recognized as important factors that contribute to the survival, colonization and persistence of EM in water distribution systems. Although the presence of EM in tap water has been linked to nosocomial infections and pseudo-infections, it remains unclear if these EM provide a health risk for immunocompromised people, in particular AIDS patients. In this regard, control strategies based on maintenance of an effective disinfectant residual and low concentration of nutrients have been proposed to keep EM numbers to a minimum in water distribution systems.

Clinical Ophthalmology, 2012

To evaluate the development of visual acuity, dysphotopsia phenomena, and subjective sensations i... more To evaluate the development of visual acuity, dysphotopsia phenomena, and subjective sensations in patients implanted with the Tecnis ® ZMB00 (Advanced Medical Optics Inc, Santa Ana, CA) multifocal intraocular lens. Methods: In a sample of 70 eyes, distance and near visual acuity, with and without correction, contrast sensitivity, and patient satisfaction, were analyzed at 15, 30, and 60 days postsurgery. Results: Mean uncorrected distance visual acuity (logarithm of minimum angle of resolution) at 15, 30, and 60 days was 0.194 ± 0.054, 0.119 ± 0.026, and 0.076 ± 0.014 (P , 0.0001), respectively, and with correction, 0.051 ± 0.007 vs 0.041 ± 0.004 vs 0.022 ± 0.002 (P , 0.0001), respectively. At 60 days, 94.3% of eyes could read 1.00 close-up without correction. Patient satisfaction in terms of dysphotopsia effects and visual acuity was excellent. The mean contrast sensitivity was 1.64 ± 0.10 (logarithmic units) measured with the Pelli-Robson test. Conclusion: This type of multifocal intraocular lens was very effective at the distances analyzed, producing excellent objective and subjective results.

EDULEARN19 Proceedings, 2019

Experimental didactic activities are absolutely necessary to achieve an adequate meaningful learn... more Experimental didactic activities are absolutely necessary to achieve an adequate meaningful learning of the Natural Sciences (NSc), also at the initial stages of primary school. Following this axiom, the last Spanish educational law pays special attention to these methodological aspects in the NSc curriculum. Therefore, an effective updating of the capabilities, scientific and practical/experimental skills of the in-service teachers is needed to give an adequate response to these demands. In order to respond to this need, we have launched the project “Aula Didàctica d'Experimentació en Ciències per a l'educació primària”, ADEC, structured as didactic experimental workshops in NSc for primary school teachers (PTs). ADEC follow the principles of the “learning by doing” methodology. This article outlines several aspects of the ADEC project, paying particular attention to its didactic methodology and scientific contents. 63% of the primary education centres (CEP) that participat...