Carlos Rose - Academia.edu (original) (raw)
Papers by Carlos Rose
PubMed, May 1, 1991
We reported that preadsorption of patient serum with heat killed E. coli increased the specificit... more We reported that preadsorption of patient serum with heat killed E. coli increased the specificity of ELISA for antibodies to Borrelia burgdorferi. That procedure required extra specimen handling and a preincubation. We report the use of a soluble E. coli antigen fraction that is included in serum diluent, eliminating additional steps. Sera from 220 individuals were tested for antibodies to B. burgdorferi. Twenty sera were obtained from patients with Lyme disease and 200 sera were from a population that included healthy controls and patients with different inflammatory conditions (viral infections and various rheumatic disorders). Testing was performed using either a standard serum diluent or one containing soluble E. coli antigen fraction. Results demonstrate that inclusion of soluble E. coli antigen fraction in serum diluent increased assay specificity from 88% for the standard protocol to 98%, with no change in test sensitivity.
![Research paper thumbnail of Corrigendum to “Granulomatous inflammation: the overlap of immune deficiency and inflammation” [Best Pract Res Clin Rheumatol 28 (2014) 191–212]](https://attachments.academia-assets.com/114217688/thumbnails/1.jpg)
](Best Practice & Research: Clinical Rheumatology, Jun 1, 2014
EMBO Reports, Apr 5, 2023
Macrophages undergo plasma membrane fusion and cell multinucleation to form multinucleated giant ... more Macrophages undergo plasma membrane fusion and cell multinucleation to form multinucleated giant cells (MGCs) such as osteoclasts in bone, Langhans giant cells (LGCs) as part of granulomas or foreign-body giant cells (FBGCs) in reaction to exogenous material. How multinucleation per se contributes to functional specialization of mature mononuclear macrophages remains poorly understood in humans. Here, we integrate comparative transcriptomics with functional assays in purified mature mononuclear and multinucleated human osteoclasts, LGCs and FBGCs. Strikingly, in all three types of MGCs, multinucleation causes a pronounced downregulation of macrophage identity. We show enhanced lysosome-mediated intracellular iron homeostasis promoting MGC formation. The transition from mononuclear to multinuclear state is accompanied by cell specialization specific to each polykaryon. Enhanced phagocytic and mitochondrial function associate with FBGCs and osteoclasts, respectively. Moreover, human LGCs preferentially express B7-H3 (CD276) and can form granuloma-like clusters in vitro, suggesting that their multinucleation potentiates T cell activation. These findings demonstrate how cell-cell fusion and multinucleation reset human macrophage identity as part of an advanced maturation step that confers MGC-specific functionality.
PubMed, Jul 1, 2001
We describe our experience with tamoxifen in a prepubertal girl with retroperitoneal fibrosis who... more We describe our experience with tamoxifen in a prepubertal girl with retroperitoneal fibrosis who had failed treatment with high dose corticosteroid therapy. Her response was excellent.
Rheumatology, Mar 1, 2005
... Checchia PA, Pahl E, Shaddy RE, Shulman ST. Cardiac transplantation for Kawasaki disease. Ped... more ... Checchia PA, Pahl E, Shaddy RE, Shulman ST. Cardiac transplantation for Kawasaki disease. Pediatrics 1997 ... Maury CP, Salo E, Pelkonen P. Elevated circulating tumor necrosis factor-alpha in patients with Kawasaki disease. J Lab ...
Rheumatology, Dec 20, 2005
Journal of Aapos, Feb 1, 1998
Chronic uveitis has been reported to occur in 13% to 34% of patients with juvenile rheumatoid art... more Chronic uveitis has been reported to occur in 13% to 34% of patients with juvenile rheumatoid arthritis (JRA). Previous studies have indicated that up to 38% of patients with JRA-associated uveitis develop severe visual impairment, with visual loss to 20/200 or worse. The authors of this publication investigated the prevalence and outcome of chronic uveitis in JRA by a retrospective analysis of 760 JRA patients followed in four pediatric rheumatology centers, Uveitis was detected in 9.3% of patients, with 15% in the pauciarticular group. This is among the lowest prevalence rates reported for patients with JRA. The authors attributed this finding to several reasons: their cohort was the largest to be analyzed for the prevalence of uveitis; the patient population was diverse; and all degrees of JRA, from mild to severe, were included in this broad primary, not tertiary, care population. The mean interval from the onset of JRAto the onset of uveitis was 21 months, and 90% of the patients who developed uveitis did so within the first 4 years of diagnosis. Patients with visual complications had a shorter interval from onset of JRA to onset of uveitis than did patients without complications. Although 31% of the children with uveitis developed complications (synechiae, band keratopathy, cataract, or glaucoma), only 11% of the uveitis patients had a visual impairment of 20/50 or less, with 5.6% suffering a visual loss to 20/100 or worse in either eye. The prevalence of uveitis was lower in the antinuclear antibody (ANA)-negative group than in the ANA-positive group. However, if uveitis occurred in ANAnegative children, complications were more common. The authors suggested that the low rate of poor visual outcome may have been the result of more aggressive screening, earlier recognition, and more effective management compared with earlier studies. They concluded that the outcome of children with JRA who developed uveitis was excellent, regardless of ANA status, 95% of the time.-JN Bloom
Annals of the Rheumatic Diseases, 2013
ABSTRACT Background The main limitation to a better knowledge of Autoinflammatory diseases is rel... more ABSTRACT Background The main limitation to a better knowledge of Autoinflammatory diseases is related to the extreme fragmentation of the diagnosed cases that are spread over different centers and countries. The general aim of the Eurofever Project (agreement n 2007332, EAHC) is to build an international registry on Autoinflammatory diseases. Objectives To evaluate the number of patients enrolled in the Registry in the first 24 months after starting the enrolment. Methods A web-based registry collecting baseline and clinical information on Autoinflammatory diseases is available in the member area of the PRINTO web-site. The following monogenic autoinflammatory diseases were considered: FMF, CAPS, TRAPS, mevalonate kinase deficiency (MKD), Blau’s syndrome, PAPA, DIRA, NLRP12-mediated periodic fever. Information on CRMO, Behcet’s disease, PFAPA and undefined periodic fevers were also collected. Results During the first 24 months from the beginning of the enrolment 2293 patients from 51 centers in 38 countries have been entered in the registry. At present baseline demographic information from 2293 (M:F=1121:1172) patients are available. In 1797 (78%) complete information on clinical manifestations form disease onset to diagnosis and response to treatment is also available. For each disease the number of enrolled patients is: FMF 694 pts (493 with complete clinical data); TRAPS 215 pts (180 with with complete clinical data); CAPS 180 pts (155 with complete clinical data); MKD 129 pts (107 with complete clinical data); Blau’s disease 30 pts (18 with complete clinical data); PAPA 8 pts (all with complete clinical data); NLRP-12 mediated periodic fever 6 pts – 4 with complete clinical data); DIRA 3 pts (all with complete clinical data). In total 19 new mutations have been reported in 22 pts. Among multifactorial autoinflammatory diseases: PFAPA 405 pts (264 with complete clinical data); CRMO 372 pts (334 with complete clinical data); pediatric Bechet disease 79 pts (64 with complete clinical data) and 173 patients with undefined periodic fever (167 with complete clinical data). Conclusions A common registry for collection of patients with Autoinflammatory disease is available and the enrolment is ongoing. The analysis of data on the clinical presentation, outcome and response to treatment of Autoinflammatory diseases and comparative studies among different Autoinflammatory conditions are ongoing. Disclosure of Interest None Declared
FDR_results_table (XLSX 11 kb)
Technical report, 2016
Childhood sarcoidosis is a general term that encompasses two quite distinct phenotypes, namely a ... more Childhood sarcoidosis is a general term that encompasses two quite distinct phenotypes, namely a familial disease with a predominantly rheumatic phenotype, Blau syndrome, and a sporadic form with multisystemic compromise that mimics the adult form of the disease. Blau syndrome is associated with a mutation in a receptor of the innate immune system NOD2 while adult sarcoidosis has no known genetic cause. This chapter provides a clinical and pathologic overview of both and focuses the pathogenic discussion on Blau syndrome.
Archivos Argentinos De Pediatria, Jun 1, 2013
PubMed, May 1, 1991
We reported that preadsorption of patient serum with heat killed E. coli increased the specificit... more We reported that preadsorption of patient serum with heat killed E. coli increased the specificity of ELISA for antibodies to Borrelia burgdorferi. That procedure required extra specimen handling and a preincubation. We report the use of a soluble E. coli antigen fraction that is included in serum diluent, eliminating additional steps. Sera from 220 individuals were tested for antibodies to B. burgdorferi. Twenty sera were obtained from patients with Lyme disease and 200 sera were from a population that included healthy controls and patients with different inflammatory conditions (viral infections and various rheumatic disorders). Testing was performed using either a standard serum diluent or one containing soluble E. coli antigen fraction. Results demonstrate that inclusion of soluble E. coli antigen fraction in serum diluent increased assay specificity from 88% for the standard protocol to 98%, with no change in test sensitivity.
Best Practice & Research: Clinical Rheumatology, Jun 1, 2014
EMBO Reports, Apr 5, 2023
Macrophages undergo plasma membrane fusion and cell multinucleation to form multinucleated giant ... more Macrophages undergo plasma membrane fusion and cell multinucleation to form multinucleated giant cells (MGCs) such as osteoclasts in bone, Langhans giant cells (LGCs) as part of granulomas or foreign-body giant cells (FBGCs) in reaction to exogenous material. How multinucleation per se contributes to functional specialization of mature mononuclear macrophages remains poorly understood in humans. Here, we integrate comparative transcriptomics with functional assays in purified mature mononuclear and multinucleated human osteoclasts, LGCs and FBGCs. Strikingly, in all three types of MGCs, multinucleation causes a pronounced downregulation of macrophage identity. We show enhanced lysosome-mediated intracellular iron homeostasis promoting MGC formation. The transition from mononuclear to multinuclear state is accompanied by cell specialization specific to each polykaryon. Enhanced phagocytic and mitochondrial function associate with FBGCs and osteoclasts, respectively. Moreover, human LGCs preferentially express B7-H3 (CD276) and can form granuloma-like clusters in vitro, suggesting that their multinucleation potentiates T cell activation. These findings demonstrate how cell-cell fusion and multinucleation reset human macrophage identity as part of an advanced maturation step that confers MGC-specific functionality.
PubMed, Jul 1, 2001
We describe our experience with tamoxifen in a prepubertal girl with retroperitoneal fibrosis who... more We describe our experience with tamoxifen in a prepubertal girl with retroperitoneal fibrosis who had failed treatment with high dose corticosteroid therapy. Her response was excellent.
Rheumatology, Mar 1, 2005
... Checchia PA, Pahl E, Shaddy RE, Shulman ST. Cardiac transplantation for Kawasaki disease. Ped... more ... Checchia PA, Pahl E, Shaddy RE, Shulman ST. Cardiac transplantation for Kawasaki disease. Pediatrics 1997 ... Maury CP, Salo E, Pelkonen P. Elevated circulating tumor necrosis factor-alpha in patients with Kawasaki disease. J Lab ...
Rheumatology, Dec 20, 2005
Journal of Aapos, Feb 1, 1998
Chronic uveitis has been reported to occur in 13% to 34% of patients with juvenile rheumatoid art... more Chronic uveitis has been reported to occur in 13% to 34% of patients with juvenile rheumatoid arthritis (JRA). Previous studies have indicated that up to 38% of patients with JRA-associated uveitis develop severe visual impairment, with visual loss to 20/200 or worse. The authors of this publication investigated the prevalence and outcome of chronic uveitis in JRA by a retrospective analysis of 760 JRA patients followed in four pediatric rheumatology centers, Uveitis was detected in 9.3% of patients, with 15% in the pauciarticular group. This is among the lowest prevalence rates reported for patients with JRA. The authors attributed this finding to several reasons: their cohort was the largest to be analyzed for the prevalence of uveitis; the patient population was diverse; and all degrees of JRA, from mild to severe, were included in this broad primary, not tertiary, care population. The mean interval from the onset of JRAto the onset of uveitis was 21 months, and 90% of the patients who developed uveitis did so within the first 4 years of diagnosis. Patients with visual complications had a shorter interval from onset of JRA to onset of uveitis than did patients without complications. Although 31% of the children with uveitis developed complications (synechiae, band keratopathy, cataract, or glaucoma), only 11% of the uveitis patients had a visual impairment of 20/50 or less, with 5.6% suffering a visual loss to 20/100 or worse in either eye. The prevalence of uveitis was lower in the antinuclear antibody (ANA)-negative group than in the ANA-positive group. However, if uveitis occurred in ANAnegative children, complications were more common. The authors suggested that the low rate of poor visual outcome may have been the result of more aggressive screening, earlier recognition, and more effective management compared with earlier studies. They concluded that the outcome of children with JRA who developed uveitis was excellent, regardless of ANA status, 95% of the time.-JN Bloom
Annals of the Rheumatic Diseases, 2013
ABSTRACT Background The main limitation to a better knowledge of Autoinflammatory diseases is rel... more ABSTRACT Background The main limitation to a better knowledge of Autoinflammatory diseases is related to the extreme fragmentation of the diagnosed cases that are spread over different centers and countries. The general aim of the Eurofever Project (agreement n 2007332, EAHC) is to build an international registry on Autoinflammatory diseases. Objectives To evaluate the number of patients enrolled in the Registry in the first 24 months after starting the enrolment. Methods A web-based registry collecting baseline and clinical information on Autoinflammatory diseases is available in the member area of the PRINTO web-site. The following monogenic autoinflammatory diseases were considered: FMF, CAPS, TRAPS, mevalonate kinase deficiency (MKD), Blau’s syndrome, PAPA, DIRA, NLRP12-mediated periodic fever. Information on CRMO, Behcet’s disease, PFAPA and undefined periodic fevers were also collected. Results During the first 24 months from the beginning of the enrolment 2293 patients from 51 centers in 38 countries have been entered in the registry. At present baseline demographic information from 2293 (M:F=1121:1172) patients are available. In 1797 (78%) complete information on clinical manifestations form disease onset to diagnosis and response to treatment is also available. For each disease the number of enrolled patients is: FMF 694 pts (493 with complete clinical data); TRAPS 215 pts (180 with with complete clinical data); CAPS 180 pts (155 with complete clinical data); MKD 129 pts (107 with complete clinical data); Blau’s disease 30 pts (18 with complete clinical data); PAPA 8 pts (all with complete clinical data); NLRP-12 mediated periodic fever 6 pts – 4 with complete clinical data); DIRA 3 pts (all with complete clinical data). In total 19 new mutations have been reported in 22 pts. Among multifactorial autoinflammatory diseases: PFAPA 405 pts (264 with complete clinical data); CRMO 372 pts (334 with complete clinical data); pediatric Bechet disease 79 pts (64 with complete clinical data) and 173 patients with undefined periodic fever (167 with complete clinical data). Conclusions A common registry for collection of patients with Autoinflammatory disease is available and the enrolment is ongoing. The analysis of data on the clinical presentation, outcome and response to treatment of Autoinflammatory diseases and comparative studies among different Autoinflammatory conditions are ongoing. Disclosure of Interest None Declared
FDR_results_table (XLSX 11 kb)
Technical report, 2016
Childhood sarcoidosis is a general term that encompasses two quite distinct phenotypes, namely a ... more Childhood sarcoidosis is a general term that encompasses two quite distinct phenotypes, namely a familial disease with a predominantly rheumatic phenotype, Blau syndrome, and a sporadic form with multisystemic compromise that mimics the adult form of the disease. Blau syndrome is associated with a mutation in a receptor of the innate immune system NOD2 while adult sarcoidosis has no known genetic cause. This chapter provides a clinical and pathologic overview of both and focuses the pathogenic discussion on Blau syndrome.
Archivos Argentinos De Pediatria, Jun 1, 2013