Pasquale Carlucci - Academia.edu (original) (raw)
Papers by Pasquale Carlucci
Journal of Chromatography B-analytical Technologies in The Biomedical and Life Sciences, 2005
A method based on solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) ... more A method based on solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) was developed for the simultaneous determination of 3-(3,5-diclorophenyl)-5-ethenyl-5-methyl-2,4-oxazolidinedione (vinclozolin) and 3-(3,5-diclorophenyl)-N-(1-methylethyl)-2,4-dioxo-1-imidazolidinecarboxamide (iprodione) in human urine. Urine samples containing vinclozolin and iprodione were collected by solid phase extraction using C(18) cartridges. The chromatographic separation was achieved on a Spherisorb ODS2 (250 mm x 4.6 mm, 5 microm) column with an isocratic mobile phase of acetonitrile-water (60:40, v/v). Detection was UV absorbance at 220 nm. The calibration graphs were linear from 30 to 1000 ng/mL for the two fungicides. Intra- and inter-day R.S.D. did not exceed 2.9%. The quantitation limit was 50 ng/mL for vinclozolin and 30 ng/mL for iprodione, respectively.
Biomedical Chromatography, 2007
A high-performance liquid chromatographic method for the simultaneous determination of di(2-ethyl... more A high-performance liquid chromatographic method for the simultaneous determination of di(2-ethylhexyl)phthalate (DEHP) and its major metabolite mono(2-ethylhexyl)phthalate (MEHP) in seminal plasma was developed and validated. The method involves liquid–liquid extraction followed by isocratic reversed-phase chromatography with diode-array detection. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from the analysis of spiked seminal plasma samples. The effect of mobile-phase composition and pH on the retention of the target analytes was investigated. The limits of detection were 0.010 and 0.015 µg/mL, for DEHP and MEHP, respectively. This method was used to analyze real samples in support of clinical studies on these potential endocrine disruptors. Copyright © 2007 John Wiley & Sons, Ltd.
Projectiles with free spinning bases are often used for smart munitions to provide effective cont... more Projectiles with free spinning bases are often used for smart munitions to provide effective control, stability and terminal guidance. Computational investigations are performed for flow past cylinders aligned along their axis where a base freely spins while attached to and separated at various distances from a non-spinning fore-body. The energy spectrum is modified to incorporate the effects of swirl and rotation using a parametric characterization of the model coefficients. An efficient finite-volume algorithm is used to solve the time-averaged equations of motion and energy along with the modeled form of transport equations for the turbulence kinetic energy and the scalar form of turbulence dissipation. Computations are performed for both rigid cylinders as well as cylinders with free-spinning bases. Experimental data for a range of spin rates and free stream flow conditions obtained from subsonic wind tunnel with sting-mounted spinning cylinders is used for validating the computational findings.
Flow past cylinders aligned along their axis where a base freely spins while attached to a non-sp... more Flow past cylinders aligned along their axis where a base freely spins while attached to a non-spinning forebody is considered from a computational and experimental point of view. The time-averaged equations of motion and energy are solved using the modeled form of transport equations for the turbulence kinetic energy and the scalar form of turbulence dissipation with an efficient finite-volume algorithm. An anisotropic two-equation Reynolds-stress model that incorporates the effect of rotation-modified energy spectrum and swirl is used to perform computations for the flow past axially rotating cylinders. Both rigid cylinders as well as that of cylinders with free-spinning base are considered from a computational point of view. A subsonic wind tunnel with a forward-sting mounted spinning cylinder is used for experiments. Experiments are performed for a range of spin rates and free stream flow conditions. The experimental results of Carlucci & Thangam (2001) are used to benchmark flow over spinning cylinders. The data is extended to munitions spinning in the wake of other munitions. Applications involving the design of projectiles are discussed.
Experimental and computational investigations of turbulent flow past projectiles is modeled as ax... more Experimental and computational investigations of turbulent flow past projectiles is modeled as axial flow past a cylinder with a free-spinning base. A subsonic wind tunnel with a forward-sting mounted spinning cylinder is used for experiments. In addition, a free-jet facility is used for benchmarking the experimental set up. Experiments are performed for a range of spin rates and free stream flow conditions. An anisotropic two-equation Reynolds-stress model that incorporates the effect of rotation-modified energy spectrum and swirl is used to perform computations for the flow past axially rotating cylinders. Both rigid cylinders as well as that of cylinders with free-spinning base are considered from a computational point of view. Applications involving the design of projectiles are discussed.
Journal of Chromatography B-analytical Technologies in The Biomedical and Life Sciences, 2005
A method based on solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) ... more A method based on solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) was developed for the simultaneous determination of 3-(3,5-diclorophenyl)-5-ethenyl-5-methyl-2,4-oxazolidinedione (vinclozolin) and 3-(3,5-diclorophenyl)-N-(1-methylethyl)-2,4-dioxo-1-imidazolidinecarboxamide (iprodione) in human urine. Urine samples containing vinclozolin and iprodione were collected by solid phase extraction using C(18) cartridges. The chromatographic separation was achieved on a Spherisorb ODS2 (250 mm x 4.6 mm, 5 microm) column with an isocratic mobile phase of acetonitrile-water (60:40, v/v). Detection was UV absorbance at 220 nm. The calibration graphs were linear from 30 to 1000 ng/mL for the two fungicides. Intra- and inter-day R.S.D. did not exceed 2.9%. The quantitation limit was 50 ng/mL for vinclozolin and 30 ng/mL for iprodione, respectively.
Biomedical Chromatography, 2007
A high-performance liquid chromatographic method for the simultaneous determination of di(2-ethyl... more A high-performance liquid chromatographic method for the simultaneous determination of di(2-ethylhexyl)phthalate (DEHP) and its major metabolite mono(2-ethylhexyl)phthalate (MEHP) in seminal plasma was developed and validated. The method involves liquid–liquid extraction followed by isocratic reversed-phase chromatography with diode-array detection. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from the analysis of spiked seminal plasma samples. The effect of mobile-phase composition and pH on the retention of the target analytes was investigated. The limits of detection were 0.010 and 0.015 µg/mL, for DEHP and MEHP, respectively. This method was used to analyze real samples in support of clinical studies on these potential endocrine disruptors. Copyright © 2007 John Wiley & Sons, Ltd.
Projectiles with free spinning bases are often used for smart munitions to provide effective cont... more Projectiles with free spinning bases are often used for smart munitions to provide effective control, stability and terminal guidance. Computational investigations are performed for flow past cylinders aligned along their axis where a base freely spins while attached to and separated at various distances from a non-spinning fore-body. The energy spectrum is modified to incorporate the effects of swirl and rotation using a parametric characterization of the model coefficients. An efficient finite-volume algorithm is used to solve the time-averaged equations of motion and energy along with the modeled form of transport equations for the turbulence kinetic energy and the scalar form of turbulence dissipation. Computations are performed for both rigid cylinders as well as cylinders with free-spinning bases. Experimental data for a range of spin rates and free stream flow conditions obtained from subsonic wind tunnel with sting-mounted spinning cylinders is used for validating the computational findings.
Flow past cylinders aligned along their axis where a base freely spins while attached to a non-sp... more Flow past cylinders aligned along their axis where a base freely spins while attached to a non-spinning forebody is considered from a computational and experimental point of view. The time-averaged equations of motion and energy are solved using the modeled form of transport equations for the turbulence kinetic energy and the scalar form of turbulence dissipation with an efficient finite-volume algorithm. An anisotropic two-equation Reynolds-stress model that incorporates the effect of rotation-modified energy spectrum and swirl is used to perform computations for the flow past axially rotating cylinders. Both rigid cylinders as well as that of cylinders with free-spinning base are considered from a computational point of view. A subsonic wind tunnel with a forward-sting mounted spinning cylinder is used for experiments. Experiments are performed for a range of spin rates and free stream flow conditions. The experimental results of Carlucci & Thangam (2001) are used to benchmark flow over spinning cylinders. The data is extended to munitions spinning in the wake of other munitions. Applications involving the design of projectiles are discussed.
Experimental and computational investigations of turbulent flow past projectiles is modeled as ax... more Experimental and computational investigations of turbulent flow past projectiles is modeled as axial flow past a cylinder with a free-spinning base. A subsonic wind tunnel with a forward-sting mounted spinning cylinder is used for experiments. In addition, a free-jet facility is used for benchmarking the experimental set up. Experiments are performed for a range of spin rates and free stream flow conditions. An anisotropic two-equation Reynolds-stress model that incorporates the effect of rotation-modified energy spectrum and swirl is used to perform computations for the flow past axially rotating cylinders. Both rigid cylinders as well as that of cylinders with free-spinning base are considered from a computational point of view. Applications involving the design of projectiles are discussed.