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Papers by Carmen Acosta

Research paper thumbnail of IgG antibodies to dengue in dry blood samples in filter paper

Revista Cenic Ciencias Biologicas, 2003

Research paper thumbnail of UltramicroELISA para la detecci�n de IgM anti dengue con reactivos listos para el uso

Research paper thumbnail of UltramicroELISA de captura para la detección de anticuerpos IgG al dengue (UMELISA-DENGUE IgG)

Revista Cenic Ciencias Biologicas, 2003

Research paper thumbnail of Presencia de anticuerpos IgG del virus de la rubéola, virus herpes simple y citomegalovirus en embarazadas residentes en Ciudad de La Habana

Research paper thumbnail of Monoclonal Antibodies Against Hepatitis B S Antigen: Production, Characterization, and Use for Diagnosis

Hybridoma, 2000

Three different hybridoma clones secreting anti-HBsAg antibody were constructed by fusing cells o... more Three different hybridoma clones secreting anti-HBsAg antibody were constructed by fusing cells of mouse myeloma line Ag8-X63 with splenocytes from BALB/c mice immunized with recombinant HBsAg and natural HBV. The monoclonal antibodies obtained were characterized ...

Research paper thumbnail of Anticuerpo anti-toxoplasma gondii en niños cubanos

Research paper thumbnail of Toxoplasmosis entre mujeres embarazadas en Ciudad de La Habana

Research paper thumbnail of UltramicroELISA para la detección de IgM anti dengue con reactivos listos para el uso

Rev Biomed, 2005

Dengue IgM capture UltraMicroELISA test with ready-to-use reagents. Dengue is currently one of th... more Dengue IgM capture UltraMicroELISA test with ready-to-use reagents. Dengue is currently one of the most important human arboviruses. It is reported in more than 100 countries, and two and a half billion people are at risk of infection. Annually, it has been estimated that there are 50 000 000 cases of infection, with more than 500 000 hospitalized cases, and 25 000 to 50 000 deaths. Because of the emergence and re-emergence of dengue and its hemorrhagic fever, the diagnosis of this disease through laboratory tests is the first objective in any country for the differentiation of dengue from other diseases, as well as, for the support of the surveillance systems. The best choice for the diagnosis of dengue fever is an immunoglobulin M-specific capture Enzyme-linked immunosorbent assay (ELISA), due to its specificity, sensitivity, and fast performance. An example of this technique is IgM dengue ultramicro ELISA. We present the results of normalization of dengue ultramicro ELISA's new format, which includes strip plates and ready-to-use reagents. The original conditions of coating and conjugate dilution of UMELISA Dengue IgM were maintained. Both, within-run and between-run precision values were below 10%, the stability of all reagents was higher than 90%. There were no significant differences when comparing the previous kinds of reagents and strips with the new ones. Compared to hemaglutination inhibition, the sensitivity was 94.3%, the specificity was 94.1%, and the kappa concordance index was 0.91. Results indicated the ultramicro ELISA's new format maintains its reliability in the early detection of dengue.

Research paper thumbnail of Specific monoclonal antibody against human trypsin

Hybridoma and …, 2002

A monoclonal antibody (MAb) directed against human trypsin-1 has been produced by hybridization o... more A monoclonal antibody (MAb) directed against human trypsin-1 has been produced by hybridization of myeloma cells with spleen cells of BALB/c immunized mice. Antibodies were screened by ultramicro enzyme-linked immunosorbent assay (UMELISA). MAb was purified by affinity ...

Research paper thumbnail of IgG antibodies to dengue in dry blood samples in filter paper

Revista Cenic Ciencias Biologicas, 2003

Research paper thumbnail of UltramicroELISA para la detecci�n de IgM anti dengue con reactivos listos para el uso

Research paper thumbnail of UltramicroELISA de captura para la detección de anticuerpos IgG al dengue (UMELISA-DENGUE IgG)

Revista Cenic Ciencias Biologicas, 2003

Research paper thumbnail of Presencia de anticuerpos IgG del virus de la rubéola, virus herpes simple y citomegalovirus en embarazadas residentes en Ciudad de La Habana

Research paper thumbnail of Monoclonal Antibodies Against Hepatitis B S Antigen: Production, Characterization, and Use for Diagnosis

Hybridoma, 2000

Three different hybridoma clones secreting anti-HBsAg antibody were constructed by fusing cells o... more Three different hybridoma clones secreting anti-HBsAg antibody were constructed by fusing cells of mouse myeloma line Ag8-X63 with splenocytes from BALB/c mice immunized with recombinant HBsAg and natural HBV. The monoclonal antibodies obtained were characterized ...

Research paper thumbnail of Anticuerpo anti-toxoplasma gondii en niños cubanos

Research paper thumbnail of Toxoplasmosis entre mujeres embarazadas en Ciudad de La Habana

Research paper thumbnail of UltramicroELISA para la detección de IgM anti dengue con reactivos listos para el uso

Rev Biomed, 2005

Dengue IgM capture UltraMicroELISA test with ready-to-use reagents. Dengue is currently one of th... more Dengue IgM capture UltraMicroELISA test with ready-to-use reagents. Dengue is currently one of the most important human arboviruses. It is reported in more than 100 countries, and two and a half billion people are at risk of infection. Annually, it has been estimated that there are 50 000 000 cases of infection, with more than 500 000 hospitalized cases, and 25 000 to 50 000 deaths. Because of the emergence and re-emergence of dengue and its hemorrhagic fever, the diagnosis of this disease through laboratory tests is the first objective in any country for the differentiation of dengue from other diseases, as well as, for the support of the surveillance systems. The best choice for the diagnosis of dengue fever is an immunoglobulin M-specific capture Enzyme-linked immunosorbent assay (ELISA), due to its specificity, sensitivity, and fast performance. An example of this technique is IgM dengue ultramicro ELISA. We present the results of normalization of dengue ultramicro ELISA's new format, which includes strip plates and ready-to-use reagents. The original conditions of coating and conjugate dilution of UMELISA Dengue IgM were maintained. Both, within-run and between-run precision values were below 10%, the stability of all reagents was higher than 90%. There were no significant differences when comparing the previous kinds of reagents and strips with the new ones. Compared to hemaglutination inhibition, the sensitivity was 94.3%, the specificity was 94.1%, and the kappa concordance index was 0.91. Results indicated the ultramicro ELISA's new format maintains its reliability in the early detection of dengue.

Research paper thumbnail of Specific monoclonal antibody against human trypsin

Hybridoma and …, 2002

A monoclonal antibody (MAb) directed against human trypsin-1 has been produced by hybridization o... more A monoclonal antibody (MAb) directed against human trypsin-1 has been produced by hybridization of myeloma cells with spleen cells of BALB/c immunized mice. Antibodies were screened by ultramicro enzyme-linked immunosorbent assay (UMELISA). MAb was purified by affinity ...

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