Carmen Hoh - Academia.edu (original) (raw)
Papers by Carmen Hoh
Page 1. 1 THE CLINICAL EVALUATION OF THE PUTATIVE CANCER CHEMOPREVENTIVE AGENT SILYBININ IN COLOR... more Page 1. 1 THE CLINICAL EVALUATION OF THE PUTATIVE CANCER CHEMOPREVENTIVE AGENT SILYBININ IN COLORECTAL CANCER AND RESECTABLE HEPATIC METASTASES Thesis submitted for the degree of Doctor of Medicine at the University of Leicester by ...
Surgery (oxford), Aug 1, 2005
Journal of Agricultural and Food Chemistry, Mar 14, 2007
Silibinin has recently received attention as a potential cancer chemopreventive agent because of ... more Silibinin has recently received attention as a potential cancer chemopreventive agent because of its antiproliferative and anticarcinogenic effects. A simple and specific reversed-phase high-performance liquid chromatography method was developed and validated for the quantitation of silibinin in human plasma. Sample preparation involved simple protein precipitation, and separation was achieved on a Waters Atlantis C18 column with flow rate of 1.0 mL/min at 40 degrees C and UV detection at 290 nm. Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area was linear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and 1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method was utilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibinin formulated with phosphatidyl choline).
Journal of Agricultural and Food Chemistry, 2007
Silibinin has recently received attention as a potential cancer chemopreventive agent because of ... more Silibinin has recently received attention as a potential cancer chemopreventive agent because of its antiproliferative and anticarcinogenic effects. A simple and specific reversed-phase high-performance liquid chromatography method was developed and validated for the quantitation of silibinin in human plasma. Sample preparation involved simple protein precipitation, and separation was achieved on a Waters Atlantis C18 column with flow rate of 1.0 mL/min at 40 degrees C and UV detection at 290 nm. Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area was linear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and 1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method was utilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibinin formulated with phosphatidyl choline).
Silybinin and its crude form silymarin exhibit cancer chemopreventive efficacy in rodents includi... more Silybinin and its crude form silymarin exhibit cancer chemopreventive efficacy in rodents including models of colorectal carcinogenesis. Silymarin is used clinically as a hepato-protectant against alcohol- and drug-related damage. Silybinin is a strong antioxidant and modulates the insulin-like growth factor (IGF) system in mice in vivo by increasing circulating levels of IGF binding protein-3 (IGFBP-3). In this thesis, the hypothesis that oral consumption of silybinin affords pharmacologically active levels in blood, liver and colorectum was tested. Twelve patients with colorectal carcinoma and twelve with colorectal liver metastases received silybinin phosphatidylcholine (silipide) at varying doses for 7 days. Blood, normal and malignant colorectal or liver tissue were obtained before and after silybinin ingestion. A HPLC-UV method was developed and validated prior to quantifying levels of silybinin in plasma and tissue samples. Plasma metabolites were identified by liquid chromat...
Page 1. 1 THE CLINICAL EVALUATION OF THE PUTATIVE CANCER CHEMOPREVENTIVE AGENT SILYBININ IN COLOR... more Page 1. 1 THE CLINICAL EVALUATION OF THE PUTATIVE CANCER CHEMOPREVENTIVE AGENT SILYBININ IN COLORECTAL CANCER AND RESECTABLE HEPATIC METASTASES Thesis submitted for the degree of Doctor of Medicine at the University of Leicester by ...
The Foundation Years, 2006
Clinical Cancer Research, 2006
Silibinin, a flavonolignan from milk thistle, has intestinal cancer chemopreventive efficacy in r... more Silibinin, a flavonolignan from milk thistle, has intestinal cancer chemopreventive efficacy in rodents. It is a strong antioxidant and modulates the insulin-like growth factor (IGF) system by increasing circulating levels of IGF-binding protein 3 (IGFBP-3) and decreasing levels of IGF-I. Here, the hypothesis was tested that administration of oral silibinin generates agent levels in human blood and colorectal and hepatic tissues consistent with pharmacologic activity. Patients with confirmed colorectal adenocarcinoma received silibinin formulated with phosphatidylcholine (silipide) at dosages of 360, 720, or 1,440 mg silibinin daily for 7 days. Blood and biopsy samples of normal and malignant colorectum or liver were obtained before dosing, and blood and colorectal or hepatic tissues were collected at resection surgery after the final silipide dose. Levels of silibinin were quantified by high-pressure liquid chromatography-UV, and plasma metabolites were identified by liquid chromatography-mass spectrometry. Blood levels of IGFBP-3, IGF-I, and the oxidative DNA damage pyrimidopurinone adduct of deoxyguanosine (M1dG) were determined. Repeated administration of silipide was safe and achieved levels of silibinin of 0.3 to 4 micromol/L in the plasma, 0.3 to 2.5 nmol/g tissue in the liver, and 20 to 141 nmol/g tissue in colorectal tissue. Silibinin monoglucuronide, silibinin diglucuronide, silibinin monosulfate, and silibinin glucuronide sulfate were identified in the plasma. Intervention with silipide did not affect circulating levels of IGFBP-3, IGF-I, or M1dG. The high silibinin levels achieved in the human colorectal mucosa after consumption of safe silibinin doses support its further exploration as a potential human colorectal cancer chemopreventive agent.
Journal of Agricultural and Food Chemistry, 2007
Silibinin has recently received attention as a potential cancer chemopreventive agent because of ... more Silibinin has recently received attention as a potential cancer chemopreventive agent because of its antiproliferative and anticarcinogenic effects. A simple and specific reversed-phase high-performance liquid chromatography method was developed and validated for the quantitation of silibinin in human plasma. Sample preparation involved simple protein precipitation, and separation was achieved on a Waters Atlantis C18 column with flow rate of 1.0 mL/min at 40 degrees C and UV detection at 290 nm. Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area was linear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and 1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method was utilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibinin formulated with phosphatidyl choline).
Page 1. 1 THE CLINICAL EVALUATION OF THE PUTATIVE CANCER CHEMOPREVENTIVE AGENT SILYBININ IN COLOR... more Page 1. 1 THE CLINICAL EVALUATION OF THE PUTATIVE CANCER CHEMOPREVENTIVE AGENT SILYBININ IN COLORECTAL CANCER AND RESECTABLE HEPATIC METASTASES Thesis submitted for the degree of Doctor of Medicine at the University of Leicester by ...
Surgery (oxford), Aug 1, 2005
Journal of Agricultural and Food Chemistry, Mar 14, 2007
Silibinin has recently received attention as a potential cancer chemopreventive agent because of ... more Silibinin has recently received attention as a potential cancer chemopreventive agent because of its antiproliferative and anticarcinogenic effects. A simple and specific reversed-phase high-performance liquid chromatography method was developed and validated for the quantitation of silibinin in human plasma. Sample preparation involved simple protein precipitation, and separation was achieved on a Waters Atlantis C18 column with flow rate of 1.0 mL/min at 40 degrees C and UV detection at 290 nm. Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area was linear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and 1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method was utilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibinin formulated with phosphatidyl choline).
Journal of Agricultural and Food Chemistry, 2007
Silibinin has recently received attention as a potential cancer chemopreventive agent because of ... more Silibinin has recently received attention as a potential cancer chemopreventive agent because of its antiproliferative and anticarcinogenic effects. A simple and specific reversed-phase high-performance liquid chromatography method was developed and validated for the quantitation of silibinin in human plasma. Sample preparation involved simple protein precipitation, and separation was achieved on a Waters Atlantis C18 column with flow rate of 1.0 mL/min at 40 degrees C and UV detection at 290 nm. Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area was linear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and 1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method was utilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibinin formulated with phosphatidyl choline).
Silybinin and its crude form silymarin exhibit cancer chemopreventive efficacy in rodents includi... more Silybinin and its crude form silymarin exhibit cancer chemopreventive efficacy in rodents including models of colorectal carcinogenesis. Silymarin is used clinically as a hepato-protectant against alcohol- and drug-related damage. Silybinin is a strong antioxidant and modulates the insulin-like growth factor (IGF) system in mice in vivo by increasing circulating levels of IGF binding protein-3 (IGFBP-3). In this thesis, the hypothesis that oral consumption of silybinin affords pharmacologically active levels in blood, liver and colorectum was tested. Twelve patients with colorectal carcinoma and twelve with colorectal liver metastases received silybinin phosphatidylcholine (silipide) at varying doses for 7 days. Blood, normal and malignant colorectal or liver tissue were obtained before and after silybinin ingestion. A HPLC-UV method was developed and validated prior to quantifying levels of silybinin in plasma and tissue samples. Plasma metabolites were identified by liquid chromat...
Page 1. 1 THE CLINICAL EVALUATION OF THE PUTATIVE CANCER CHEMOPREVENTIVE AGENT SILYBININ IN COLOR... more Page 1. 1 THE CLINICAL EVALUATION OF THE PUTATIVE CANCER CHEMOPREVENTIVE AGENT SILYBININ IN COLORECTAL CANCER AND RESECTABLE HEPATIC METASTASES Thesis submitted for the degree of Doctor of Medicine at the University of Leicester by ...
The Foundation Years, 2006
Clinical Cancer Research, 2006
Silibinin, a flavonolignan from milk thistle, has intestinal cancer chemopreventive efficacy in r... more Silibinin, a flavonolignan from milk thistle, has intestinal cancer chemopreventive efficacy in rodents. It is a strong antioxidant and modulates the insulin-like growth factor (IGF) system by increasing circulating levels of IGF-binding protein 3 (IGFBP-3) and decreasing levels of IGF-I. Here, the hypothesis was tested that administration of oral silibinin generates agent levels in human blood and colorectal and hepatic tissues consistent with pharmacologic activity. Patients with confirmed colorectal adenocarcinoma received silibinin formulated with phosphatidylcholine (silipide) at dosages of 360, 720, or 1,440 mg silibinin daily for 7 days. Blood and biopsy samples of normal and malignant colorectum or liver were obtained before dosing, and blood and colorectal or hepatic tissues were collected at resection surgery after the final silipide dose. Levels of silibinin were quantified by high-pressure liquid chromatography-UV, and plasma metabolites were identified by liquid chromatography-mass spectrometry. Blood levels of IGFBP-3, IGF-I, and the oxidative DNA damage pyrimidopurinone adduct of deoxyguanosine (M1dG) were determined. Repeated administration of silipide was safe and achieved levels of silibinin of 0.3 to 4 micromol/L in the plasma, 0.3 to 2.5 nmol/g tissue in the liver, and 20 to 141 nmol/g tissue in colorectal tissue. Silibinin monoglucuronide, silibinin diglucuronide, silibinin monosulfate, and silibinin glucuronide sulfate were identified in the plasma. Intervention with silipide did not affect circulating levels of IGFBP-3, IGF-I, or M1dG. The high silibinin levels achieved in the human colorectal mucosa after consumption of safe silibinin doses support its further exploration as a potential human colorectal cancer chemopreventive agent.
Journal of Agricultural and Food Chemistry, 2007
Silibinin has recently received attention as a potential cancer chemopreventive agent because of ... more Silibinin has recently received attention as a potential cancer chemopreventive agent because of its antiproliferative and anticarcinogenic effects. A simple and specific reversed-phase high-performance liquid chromatography method was developed and validated for the quantitation of silibinin in human plasma. Sample preparation involved simple protein precipitation, and separation was achieved on a Waters Atlantis C18 column with flow rate of 1.0 mL/min at 40 degrees C and UV detection at 290 nm. Silibinin was detected as two peaks corresponding to trans-diastereoisomers. The peak area was linear over the investigated concentration range (0-5000 ng/mL). The limits of detection were 2 and 1 ng/mL for the two diastereoisomers (d1 and d2), with a recovery of 53-58%. This method was utilized to detect silibinin in plasma of colorectal patients after 7 days of treatment with silipide (silibinin formulated with phosphatidyl choline).