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Papers by Miguel Castellano

Research paper thumbnail of The effects of isonicotinic acid hydrazide on the early chick embryo

Development, 1973

Unincubated White Leghorn fertile eggs were injected with isonicotinic acid hydrazide (INH) throu... more Unincubated White Leghorn fertile eggs were injected with isonicotinic acid hydrazide (INH) through a hole in the shell. A control group was injected with normal saline, a second group with INH plus vitamin B6, and a third group was left unopened. INH, in the doses we used, proved to be lethal for more than 50% of the early chick embryos, and also produced important developmental alterations. Alterations produced by INH were primarily observed at the level of the neural epithe-lium, particularly at its cephalic portion. The most important ones were a degenerative and necrotic process of the neural epithelium, and a distortion of the normal anatomical relations of the cephalic structures. The embryonic mortality and developmental alterations induced by INH were prevented to a considerable extent by the concurrent injection of pyridoxine hydrochloride and INH. The preventive action of vitamin B6 suggests that toxicity of INH in the chicken embryos is due to the antivitamin-B6 action o...

Research paper thumbnail of The histochemical distribution of fructose metabolism enzymes in bovine spermatogenesis

Research paper thumbnail of IMG 20181122 103749. Oral absorption of gonadotropin

Research paper thumbnail of Therapeutic Method of Treatment for Inhibiting Tumor Development Using Tamm-Horsfall Glycoprotein (THP) as Immunostimulating Drug and Compositions

Research paper thumbnail of Histochemical detection of several enzymes in Crytomphallus and Blaptica and its relation to neuromuscular transmission

Abstract 1. 1. Histochemical techniques for the demonstration of glutamate dehydrogenase (GDH), s... more Abstract 1. 1. Histochemical techniques for the demonstration of glutamate dehydrogenase (GDH), succinate dehydrogenase (SDH), cholinesterases (ChEase) and phosphorylase were performed in the pharyngeal retractor muscle of the snail (Cryptomphallus) and in the muscles of the cockroach leg (Blaptica). 2. 2. In the smooth muscle of snail the presence of the four reactions in the sarcolemma and sarcoplasm stands out. 3. 3. In the striated muscle of the cockroach leg a positive reaction for dehydrogenases and cholinesterases is obtained in the sarcolemma and sarcoplasm. 4. 4. The meaning of these results in relation to neuromuscular transmission and findings of electron microscopy is discussed.

Research paper thumbnail of Ram Spermatozoa Studied with Techniques for the Cytochemical Detection of Phosphorylase and Succinic Dehydrogenase

Research paper thumbnail of Histochemical Studies of Bovine Seminal Vesicles During Postnatal Development

Journal of Endocrinology, 1971

SUMMARY The location of aldose and ketose reductases and of isocitric, succinic, malic and α-keto... more SUMMARY The location of aldose and ketose reductases and of isocitric, succinic, malic and α-ketoglutaric dehydrogenases was studied by histochemical methods in the seminal vesicles of the bull, bull calf and castrated bulls. The appearance of aldose and ketose reductase activity coincides with that of significant quantities of fructose collected from the accessory glands of calves of 5·5 months of age. The enzymes of the Krebs' cycle, isocitric, succinic, malic and α-ketoglutaric dehydrogenases, could not be histochemically demonstrated in the seminal vesicles of bulls, bull calves and castrated young bulls. The factors determining the high citric acid concentrations in bull seminal vesicles are discussed.

Research paper thumbnail of Histochemical demonstration of l-amino acid-tetrazolium reductase

Histochemie, 1969

A method for the histochemical demonstration of l-amino acid-tetrazolium reductase is described. ... more A method for the histochemical demonstration of l-amino acid-tetrazolium reductase is described. The diformazan deposition was obtained with l-leucine as substrate and no precipitate was present when l-serine and l-lisine were used. In the kidney the reaction was positive in the podocytes, the cells of proximal and distal convoluted tubules, in the ascending limbs of Henle and in the cells

Research paper thumbnail of Effect of experimental cryptorchism on testis L-iditol: NAD oxidoreductase (sorbitol dehydrogenase or ketose-reductase)

Histochemie, 1973

The histochemical distribution of sorbitol dehydrogenase in normal and cryptorchid rat testis has... more The histochemical distribution of sorbitol dehydrogenase in normal and cryptorchid rat testis has been studied. In the normal testis sorbitol dehydrogenase is localized in the spermatids, increases during their differentiation and is maximal in those spermatids attached to the Sertoli cells (stages V-VII). In the cryptorehid testis, sorbitol dehydrogenase activity of the spermatids, similarly to that of the Sertoli cells, is completely abolished. There. fore, we conclude that sorhitol dehydrogenase activity of the Sertoli cells depends on the spermatid differentiation.

Research paper thumbnail of Biological and immunological studies of a placental gonadotropic protein

European Journal of Obstetrics & Gynecology and Reproductive Biology, 1976

Precocious sexual maturity, ovarian weight increase and stimulation of the sex accessory organs i... more Precocious sexual maturity, ovarian weight increase and stimulation of the sex accessory organs in impuber female mice, was induced by a partially purified protein fraction obtained from human placenta. The relationship between this biologically active protein and HCG was studied by a comparative immunodouble diffusion technique (Ouchterlony) and two agglutination inhibition tests. Results showed that this protein with gonadotropic activity is neither immunologically related to HCG nor contaminated with this hormone. gonadotropins; placenta _ * This paper has been partially supported by research grant 6.8.1.75 from the Progama Latinoamericano de Investigaciones en Reproduction Humana (PLAMIRH).

Research paper thumbnail of Chemical and biological characterization of an active substance of the human placenta

European Journal of Obstetrics & Gynecology and Reproductive Biology, 1976

Research paper thumbnail of Role of the nuclear membrane in smooth endoplasmic reticulum formation in white rat pinealocytes

Research paper thumbnail of Effects of iproniazid on chick development

Cells Tissues Organs, 1975

New Hampshire eggs were injected with iproniazid into the yolk sac at day 2 of incubation by mean... more New Hampshire eggs were injected with iproniazid into the yolk sac at day 2 of incubation by means of a tiny hole in the shell. A control group was injected with normal saline and a second group was left unopened. Iproniazid in the doses we used proved to be lethal for approximately 50% of the chick embryos, while we cannot demonstrate other developmental alterations, either in the macroscopical or in the serial histological study.

Research paper thumbnail of Histochemical studies on phosphorylase activity in the tissues of the albino rat under normal and experimental conditions

Cells Tissues Organs, 1971

Research paper thumbnail of Location of L-Iditol: Nad Oxidoreductase (D-Sorbitol Tetrazolium Reductase or Ketose-Reductase) in the Genital Tract of the Bull

Journal of Endocrinology, 1970

SUMMARY The histochemical technique for the demonstration of the d-sorbitol dehydrogenase activit... more SUMMARY The histochemical technique for the demonstration of the d-sorbitol dehydrogenase activity gave intensely positive results in the epithelium of the seminal vesicles and the glands of the ampulla of the ductus deferens of the bull. The enzymic activity was lower in the prostatic epithelium of the ductus deferens and the epididymis. The reaction was negative in the epithelium of the bulbo-urethral glands of Cowper. The enzymic activity detected is connected with the synthesis of fructose, but in organs with a low concentration of fructose, such as the prostate gland and the ductus epididymidis the presence of the enzyme may be connected with the synthesis of sorbitol, also present in the semen. In the seminal vesicles d-sorbitol dehydrogenase activity was found in the epithelium of the glandular tubules and in the material contained in those tubules. Using this technique, we have observed in the epithelium different types of cells which may correspond to different stages of a ...

Research paper thumbnail of The effects of isonicotinic acid hydrazide on the early chick embryo

Development, 1973

Unincubated White Leghorn fertile eggs were injected with isonicotinic acid hydrazide (INH) throu... more Unincubated White Leghorn fertile eggs were injected with isonicotinic acid hydrazide (INH) through a hole in the shell. A control group was injected with normal saline, a second group with INH plus vitamin B6, and a third group was left unopened. INH, in the doses we used, proved to be lethal for more than 50% of the early chick embryos, and also produced important developmental alterations. Alterations produced by INH were primarily observed at the level of the neural epithe-lium, particularly at its cephalic portion. The most important ones were a degenerative and necrotic process of the neural epithelium, and a distortion of the normal anatomical relations of the cephalic structures. The embryonic mortality and developmental alterations induced by INH were prevented to a considerable extent by the concurrent injection of pyridoxine hydrochloride and INH. The preventive action of vitamin B6 suggests that toxicity of INH in the chicken embryos is due to the antivitamin-B6 action o...

Research paper thumbnail of The histochemical distribution of fructose metabolism enzymes in bovine spermatogenesis

Research paper thumbnail of IMG 20181122 103749. Oral absorption of gonadotropin

Research paper thumbnail of Therapeutic Method of Treatment for Inhibiting Tumor Development Using Tamm-Horsfall Glycoprotein (THP) as Immunostimulating Drug and Compositions

Research paper thumbnail of Histochemical detection of several enzymes in Crytomphallus and Blaptica and its relation to neuromuscular transmission

Abstract 1. 1. Histochemical techniques for the demonstration of glutamate dehydrogenase (GDH), s... more Abstract 1. 1. Histochemical techniques for the demonstration of glutamate dehydrogenase (GDH), succinate dehydrogenase (SDH), cholinesterases (ChEase) and phosphorylase were performed in the pharyngeal retractor muscle of the snail (Cryptomphallus) and in the muscles of the cockroach leg (Blaptica). 2. 2. In the smooth muscle of snail the presence of the four reactions in the sarcolemma and sarcoplasm stands out. 3. 3. In the striated muscle of the cockroach leg a positive reaction for dehydrogenases and cholinesterases is obtained in the sarcolemma and sarcoplasm. 4. 4. The meaning of these results in relation to neuromuscular transmission and findings of electron microscopy is discussed.

Research paper thumbnail of Ram Spermatozoa Studied with Techniques for the Cytochemical Detection of Phosphorylase and Succinic Dehydrogenase

Research paper thumbnail of Histochemical Studies of Bovine Seminal Vesicles During Postnatal Development

Journal of Endocrinology, 1971

SUMMARY The location of aldose and ketose reductases and of isocitric, succinic, malic and α-keto... more SUMMARY The location of aldose and ketose reductases and of isocitric, succinic, malic and α-ketoglutaric dehydrogenases was studied by histochemical methods in the seminal vesicles of the bull, bull calf and castrated bulls. The appearance of aldose and ketose reductase activity coincides with that of significant quantities of fructose collected from the accessory glands of calves of 5·5 months of age. The enzymes of the Krebs' cycle, isocitric, succinic, malic and α-ketoglutaric dehydrogenases, could not be histochemically demonstrated in the seminal vesicles of bulls, bull calves and castrated young bulls. The factors determining the high citric acid concentrations in bull seminal vesicles are discussed.

Research paper thumbnail of Histochemical demonstration of l-amino acid-tetrazolium reductase

Histochemie, 1969

A method for the histochemical demonstration of l-amino acid-tetrazolium reductase is described. ... more A method for the histochemical demonstration of l-amino acid-tetrazolium reductase is described. The diformazan deposition was obtained with l-leucine as substrate and no precipitate was present when l-serine and l-lisine were used. In the kidney the reaction was positive in the podocytes, the cells of proximal and distal convoluted tubules, in the ascending limbs of Henle and in the cells

Research paper thumbnail of Effect of experimental cryptorchism on testis L-iditol: NAD oxidoreductase (sorbitol dehydrogenase or ketose-reductase)

Histochemie, 1973

The histochemical distribution of sorbitol dehydrogenase in normal and cryptorchid rat testis has... more The histochemical distribution of sorbitol dehydrogenase in normal and cryptorchid rat testis has been studied. In the normal testis sorbitol dehydrogenase is localized in the spermatids, increases during their differentiation and is maximal in those spermatids attached to the Sertoli cells (stages V-VII). In the cryptorehid testis, sorbitol dehydrogenase activity of the spermatids, similarly to that of the Sertoli cells, is completely abolished. There. fore, we conclude that sorhitol dehydrogenase activity of the Sertoli cells depends on the spermatid differentiation.

Research paper thumbnail of Biological and immunological studies of a placental gonadotropic protein

European Journal of Obstetrics & Gynecology and Reproductive Biology, 1976

Precocious sexual maturity, ovarian weight increase and stimulation of the sex accessory organs i... more Precocious sexual maturity, ovarian weight increase and stimulation of the sex accessory organs in impuber female mice, was induced by a partially purified protein fraction obtained from human placenta. The relationship between this biologically active protein and HCG was studied by a comparative immunodouble diffusion technique (Ouchterlony) and two agglutination inhibition tests. Results showed that this protein with gonadotropic activity is neither immunologically related to HCG nor contaminated with this hormone. gonadotropins; placenta _ * This paper has been partially supported by research grant 6.8.1.75 from the Progama Latinoamericano de Investigaciones en Reproduction Humana (PLAMIRH).

Research paper thumbnail of Chemical and biological characterization of an active substance of the human placenta

European Journal of Obstetrics & Gynecology and Reproductive Biology, 1976

Research paper thumbnail of Role of the nuclear membrane in smooth endoplasmic reticulum formation in white rat pinealocytes

Research paper thumbnail of Effects of iproniazid on chick development

Cells Tissues Organs, 1975

New Hampshire eggs were injected with iproniazid into the yolk sac at day 2 of incubation by mean... more New Hampshire eggs were injected with iproniazid into the yolk sac at day 2 of incubation by means of a tiny hole in the shell. A control group was injected with normal saline and a second group was left unopened. Iproniazid in the doses we used proved to be lethal for approximately 50% of the chick embryos, while we cannot demonstrate other developmental alterations, either in the macroscopical or in the serial histological study.

Research paper thumbnail of Histochemical studies on phosphorylase activity in the tissues of the albino rat under normal and experimental conditions

Cells Tissues Organs, 1971

Research paper thumbnail of Location of L-Iditol: Nad Oxidoreductase (D-Sorbitol Tetrazolium Reductase or Ketose-Reductase) in the Genital Tract of the Bull

Journal of Endocrinology, 1970

SUMMARY The histochemical technique for the demonstration of the d-sorbitol dehydrogenase activit... more SUMMARY The histochemical technique for the demonstration of the d-sorbitol dehydrogenase activity gave intensely positive results in the epithelium of the seminal vesicles and the glands of the ampulla of the ductus deferens of the bull. The enzymic activity was lower in the prostatic epithelium of the ductus deferens and the epididymis. The reaction was negative in the epithelium of the bulbo-urethral glands of Cowper. The enzymic activity detected is connected with the synthesis of fructose, but in organs with a low concentration of fructose, such as the prostate gland and the ductus epididymidis the presence of the enzyme may be connected with the synthesis of sorbitol, also present in the semen. In the seminal vesicles d-sorbitol dehydrogenase activity was found in the epithelium of the glandular tubules and in the material contained in those tubules. Using this technique, we have observed in the epithelium different types of cells which may correspond to different stages of a ...