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Papers by Caterina Casano

Nucleic Acids Research, 1979

Histone mRNAs at different stages of development were purified by hybridization with the cloned h... more Histone mRNAs at different stages of development were purified by hybridization with the cloned homologous histone genes. The electrophoretic patterns of oocytes, 2-4 blastomeres, 64 cells and morula histone mRNAs was found to be identical, whereas the electrophoretic pattern of mesenchyme blastula histone mRNA was aseddebr different. The cloned histone DNA of P.lividus was hybridized with the RNA of each stage. The Tm was 74°C in all cases except for the mesenchyme histone mRNAs whose Tm was 59°C, thus suggesting that at least two different clusters of histone genes are active in the course of the sea urchin development.

Caryologia, 2008

It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is const... more It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is constitutively expressed during development, that it increases after heat-shock and cadmium treatment, and that it has a specific territorial distribution, both in normal and heat-shocked embryos, as shown by immunolocalization experiments. In this work, we analyzed by Western blot the territorial distribution of the protein in plutei exposed to heat-shock or sublethal cadmium concentrations, and we found that Hsp56 increases in both ectodermal and endodermal cells. Moreover, by "in situ" hybridization, we looked at Hsp56 mRNA during normal development and under stress conditions. We found that the territorial distribution of the messenger changes during development and that its amount is steadily increased in stressed embryos. Finally, by T1 RNase assay, we identified a cytoplasmic factor that binds to the region of Hsp56 messenger containing the 5'UTR.

Echinoderm Research 1991, 2020

Progress in clinical and biological research, 1982

Cell Biology International Reports, 1990

Caryologia, 2008

It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is const... more It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is constitutively expressed during development, that it increases after heat-shock and cadmium treatment, and that it has a specific territorial distribution, both in normal and heat-shocked embryos, as shown by immunolocalization experiments. In this work, we analyzed by Western blot the territorial distribution of the protein in plutei exposed to heat-shock or sublethal cadmium concentrations, and we found that Hsp56 increases in both ectodermal and endodermal cells. Moreover, by "in situ" hybridization, we looked at Hsp56 mRNA during normal development and under stress conditions. We found that the territorial distribution of the messenger changes during development and that its amount is steadily increased in stressed embryos. Finally, by T1 RNase assay, we identified a cytoplasmic factor that binds to the region of Hsp56 messenger containing the 5'UTR.

European Journal of Biochemistry, 2005

Mesenchyme blastula sea‐urchin embryos were heat‐shocked at 31°C and pulse‐labelled with [3H]urid... more Mesenchyme blastula sea‐urchin embryos were heat‐shocked at 31°C and pulse‐labelled with [3H]uridine. The nuclear RNA was fractionated on glyoxal/agarose gels and each RNA fraction hybridized with cloned early blastula histone DNA. The results showed that after heat shock there is an accumulation of histone RNA molecules larger than the messenger and a decrease, with respect to the control, of 9‐S histone RNA. Chasing of the heat shock RNA by incubation of the embryos with unlabelled uridine and cytidine restores the radioactivity, as well as the hybridization profiles, of control embryos. Furthermore saturation curves obtained by hybridizing histone DNA with labelled 9‐S RNA, showed an absence of the histone messenger in the cytoplasm of heat‐shocked embryos, whereas it is present in both control and chased embryos. These results are consistent with the hypothesis that the accumulated histone RNA in heat‐shocked embryos is the precursor of mature messengers.

Proceedings of the National Academy of Sciences, 1993

To shed some light on the mechanisms involved in the coordinate regulation of the early histone g... more To shed some light on the mechanisms involved in the coordinate regulation of the early histone gene set during sea urchin development, we tested the hypothesis that the upstream sequence element USE1, previously identified in the early H2A modulator, could also participate in the transcription of the early histone H3 gene. We found by DNAse I protection analysis and by competition in electrophoretic mobility-shift experiments that two sequence elements of the H3 promoter closely resembled the USE1-H2A sequence in their binding activity for nuclear factors from 64-cell stage embryos. These modulator binding factor 1 (MBF-1)-related factors seem to recognize the ACAGA motif that is conserved between the USE1-like sequences of both H2A and H3 promoters. In fact, excess oligonucleotide containing a mutated USE1-H2A element in which the ACAGA sequence was mutated to AGTCA failed to compete with the USE1 sites of both H2A and H3 genes for interaction with MBF-1. Finally, in vivo transcri...

Journal of Histochemistry & Cytochemistry, 2003

In a previous paper we demonstrated that, in Paracentrotus lividus embryos, deciliation represent... more In a previous paper we demonstrated that, in Paracentrotus lividus embryos, deciliation represents a specific kind of stress that induces an increase in the levels of an acidic protein of about 40 kD (p40). Here we report that deciliation also induces an increase in Hsp40 chaperone levels and enhancement of its ectodermal localization. We suggest that Hsp40 might play a chaperoning role in cilia regeneration.

Nucleic Acids Research, 1979

Histone mRNAs at different stages of development were purified by hybridization with the cloned h... more Histone mRNAs at different stages of development were purified by hybridization with the cloned homologous histone genes. The electrophoretic patterns of oocytes, 2-4 blastomeres, 64 cells and morula histone mRNAs was found to be identical, whereas the electrophoretic pattern of mesenchyme blastula histone mRNA was aseddebr different. The cloned histone DNA of P.lividus was hybridized with the RNA of each stage. The Tm was 74°C in all cases except for the mesenchyme histone mRNAs whose Tm was 59°C, thus suggesting that at least two different clusters of histone genes are active in the course of the sea urchin development.

Caryologia, 2008

It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is const... more It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is constitutively expressed during development, that it increases after heat-shock and cadmium treatment, and that it has a specific territorial distribution, both in normal and heat-shocked embryos, as shown by immunolocalization experiments. In this work, we analyzed by Western blot the territorial distribution of the protein in plutei exposed to heat-shock or sublethal cadmium concentrations, and we found that Hsp56 increases in both ectodermal and endodermal cells. Moreover, by "in situ" hybridization, we looked at Hsp56 mRNA during normal development and under stress conditions. We found that the territorial distribution of the messenger changes during development and that its amount is steadily increased in stressed embryos. Finally, by T1 RNase assay, we identified a cytoplasmic factor that binds to the region of Hsp56 messenger containing the 5'UTR.

Echinoderm Research 1991, 2020

Progress in clinical and biological research, 1982

Cell Biology International Reports, 1990

Caryologia, 2008

It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is const... more It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is constitutively expressed during development, that it increases after heat-shock and cadmium treatment, and that it has a specific territorial distribution, both in normal and heat-shocked embryos, as shown by immunolocalization experiments. In this work, we analyzed by Western blot the territorial distribution of the protein in plutei exposed to heat-shock or sublethal cadmium concentrations, and we found that Hsp56 increases in both ectodermal and endodermal cells. Moreover, by "in situ" hybridization, we looked at Hsp56 mRNA during normal development and under stress conditions. We found that the territorial distribution of the messenger changes during development and that its amount is steadily increased in stressed embryos. Finally, by T1 RNase assay, we identified a cytoplasmic factor that binds to the region of Hsp56 messenger containing the 5'UTR.

European Journal of Biochemistry, 2005

Mesenchyme blastula sea‐urchin embryos were heat‐shocked at 31°C and pulse‐labelled with [3H]urid... more Mesenchyme blastula sea‐urchin embryos were heat‐shocked at 31°C and pulse‐labelled with [3H]uridine. The nuclear RNA was fractionated on glyoxal/agarose gels and each RNA fraction hybridized with cloned early blastula histone DNA. The results showed that after heat shock there is an accumulation of histone RNA molecules larger than the messenger and a decrease, with respect to the control, of 9‐S histone RNA. Chasing of the heat shock RNA by incubation of the embryos with unlabelled uridine and cytidine restores the radioactivity, as well as the hybridization profiles, of control embryos. Furthermore saturation curves obtained by hybridizing histone DNA with labelled 9‐S RNA, showed an absence of the histone messenger in the cytoplasm of heat‐shocked embryos, whereas it is present in both control and chased embryos. These results are consistent with the hypothesis that the accumulated histone RNA in heat‐shocked embryos is the precursor of mature messengers.

Proceedings of the National Academy of Sciences, 1993

To shed some light on the mechanisms involved in the coordinate regulation of the early histone g... more To shed some light on the mechanisms involved in the coordinate regulation of the early histone gene set during sea urchin development, we tested the hypothesis that the upstream sequence element USE1, previously identified in the early H2A modulator, could also participate in the transcription of the early histone H3 gene. We found by DNAse I protection analysis and by competition in electrophoretic mobility-shift experiments that two sequence elements of the H3 promoter closely resembled the USE1-H2A sequence in their binding activity for nuclear factors from 64-cell stage embryos. These modulator binding factor 1 (MBF-1)-related factors seem to recognize the ACAGA motif that is conserved between the USE1-like sequences of both H2A and H3 promoters. In fact, excess oligonucleotide containing a mutated USE1-H2A element in which the ACAGA sequence was mutated to AGTCA failed to compete with the USE1 sites of both H2A and H3 genes for interaction with MBF-1. Finally, in vivo transcri...

Journal of Histochemistry & Cytochemistry, 2003

In a previous paper we demonstrated that, in Paracentrotus lividus embryos, deciliation represent... more In a previous paper we demonstrated that, in Paracentrotus lividus embryos, deciliation represents a specific kind of stress that induces an increase in the levels of an acidic protein of about 40 kD (p40). Here we report that deciliation also induces an increase in Hsp40 chaperone levels and enhancement of its ectodermal localization. We suggest that Hsp40 might play a chaperoning role in cilia regeneration.