Caterina Matinato - Academia.edu (original) (raw)

Papers by Caterina Matinato

Critical Care

Background No univocal recommendation exists for microbiological diagnosis of ventilator-associat... more Background No univocal recommendation exists for microbiological diagnosis of ventilator-associated pneumonia (VAP). Sampling of either proximal or distal respiratory tract likely impacts on the broad range of VAP incidence between cohorts. Immune biomarkers to rule-in/rule-out VAP diagnosis, although promising, have not yet been validated. COVID-19-induced ARDS made VAP recognition even more challenging, often leading to overdiagnosis and overtreatment. We evaluated the impact of different respiratory samples and laboratory techniques on VAP incidence and microbiological findings in COVID-19 patients. Methods Prospective single-centre cohort study conducted among COVID-19 mechanically ventilated patients in Policlinico Hospital (Milan, Italy) from January 2021 to May 2022. Microbiological confirmation of suspected VAP (sVAP) was based on concomitant endotracheal aspirates (ETA) and bronchoalveolar lavage (BAL). Conventional and fast microbiology (FILMARRAY® Pneumonia Panel plus, BA...

Emerging Infectious Diseases

Microbiology Spectrum

Infection prevention and control (IPC) practices are of paramount importance for preventing the s... more Infection prevention and control (IPC) practices are of paramount importance for preventing the spread of multidrug-resistant organisms (MDROs) in hospitals, especially in the intensive care unit (ICU). Whole-genome sequencing (WGS) is seen as a promising tool for IPC, but its employment is currently still limited.

International Journal of Infectious Diseases

West Nile virus (WNV) is an arbovirus belong-ing to the Flaviviridae family and is transmitted wi... more West Nile virus (WNV) is an arbovirus belong-ing to the Flaviviridae family and is transmitted with a natural cycle that involves birds and mos-quitoes. In recent years, the spread of WNV has been observed worldwide and several outbreaks were also recorded in Europe (Lanciotti et al., 1999; Zeller et al., 2004). Since the first report of WNV infections in humans in north-east Italy in 2008, several human cases of WNV-related meningo-encephalitis were identified in Italy in the Emilia-Romagna and Veneto regions (Rossini et al., 2011), indicating an endemic circulation of this virus in the wetland of the Po river valley. As a consequence of these findings, an integrat-

Acta Myologica, 2018

Statin-induced necrotizing autoimmune myopathy (IMNM) is an autoimmune disorder induced by anti-3... more Statin-induced necrotizing autoimmune myopathy (IMNM) is an autoimmune disorder induced by anti-3-hydroxy-3-methylglutaryl-coenzyme-A reductase (anti-HMGCR) antibodies. We performed a retrospective clinical, histological, and radiological evaluation of 5 patients with a 3-year therapeutic follow-up. All patients used statins and then experienced proximal weakness that persisted after drug cessation. Muscle biopsies revealed a primary necrotizing myopathy without inflammatory infiltrates. All patients required immunomodulant combination therapy to achieve clinical remission. Magnetic resonance imaging (MRI) showed the presence of edema in the medial gastrocnemius, posterior and central loggia of the thigh, posterior loggia of the arm, and the infraspinatus and subscapularis muscles, as well as extensive inflammation of the subcutaneous tissues and muscolaris fasciae. Serum analysis, muscle biopsy, and MRI are fundamental for IMNM diagnosis and follow-up. The growing use of statins in...

European Urology Open Science, 2021

Clinical chemistry and laboratory medicine, Jan 21, 2017

Primary biliary cholangitis (PBC), also known as primary biliary cirrhosis, is an autoimmune dise... more Primary biliary cholangitis (PBC), also known as primary biliary cirrhosis, is an autoimmune disease of the liver characterized by anti-mitochondrial antibodies (AMA) in 90%-95% of patients. The aim of this study was to evaluate the diagnostic value of several serum biomarkers in patients with PBC but negative for AMA. Some antinuclear antibodies (ANA) pattern, detected by indirect immunofluorescence (IIF), such as multiple nuclear dot (MND) and rim-like patterns are well-known to be specific for PBC. The corresponding nuclear antigens are the components of the nuclear pore complex (Gp210 for rim-like pattern) and Sp100, PML proteins (for MND pattern) detectable by immunoblotting and ELISA methods. More recently, new biomarkers have been evaluated in order to improve the diagnostic sensitivity, such as kelch-like 12 (KLHL12) and hexokinase-1. Considering these different serum biomarkers, studies evaluating their diagnostic role in AMA-negative PBC patients compared to AMA-positive o...

Journal of Autoimmunity, 2016

evaluation to establish and inform women about pregnancy risks; b) planning pregnancy during inac... more evaluation to establish and inform women about pregnancy risks; b) planning pregnancy during inactive lupus nephritis, maintained inactive with the lowest possible dosage of allowed drugs; c) adequate treatment of known risk factors (arterial hypertension, antiphospholipid and antibodies); d) close monitoring during and after pregnancy to rapidly identify and treat SLE flares and obstetric complications.

Clinical chemistry and laboratory medicine : CCLM / FESCC, Jan 14, 2015

The association of anti-C1q antibodies (anti-C1q) with the renal activity of lupus nephritis (LN)... more The association of anti-C1q antibodies (anti-C1q) with the renal activity of lupus nephritis (LN) and the methods for their determination is still a matter of debate. In 116 serum samples of 66 patients with biopsy proven LN, we aimed: 1) to compare the results of the determination of anti-C1q obtained by a commercial kit with a clinically validated in-house ELISA; 2) to evaluate the correlation of anti-C1q with the most important immunological and clinical parameters employed in LN, i.e., antibodies to dsDNA (anti-dsDNA), C3 and C4 complement component, haemoglobin and haematuria. Good correlation and agreement between the two methods (r=0.81, p<0.0001; contingency coefficient=0.70, p<0.0001, respectively) were demonstrated. No differences were observed between the two assays by ROC curves comparison. Anti-C1q levels were significantly higher in patients with active LN [44 arbitrary units (AUs)] in comparison to those with inactive LN (23 AUs, p=0.047) and significantly corre...

Transplantation proceedings, 1996

Transplantation proceedings, 1994

The European journal of surgery = Acta chirurgica, 1995

To examine the effects of two immunosuppressant regimens on composition of the bowel flora and ra... more To examine the effects of two immunosuppressant regimens on composition of the bowel flora and rate of translocation after transplantation of the small bowel in pigs. Randomised controlled study. University hospital, Italy. 35 female Large White pigs. 9 Animals were not operated on (normal controls). 19 Animals underwent total orthotopic small bowel allotransplantation and were then randomised to receive: group A (n = 8) cyclosporin A 25 mg/kg subcutaneously and cephazolin 2 g intramuscularly daily; group B (n = 6) 15-deoxyspergualin (15-dos) 3 mg/kg for 7 days then 1.5 mg/kg, cephazolin 2 g intramuscularly daily for 4 days then selective intestinal decontamination with colistin 1.5 million U, tobramycin 100 mg, vancomycin 1 g, and nystatin 500,000 U daily; and group C (n = 5) cephazolin 2 g intramuscularly daily for 8 days. A further group (D, n = 7) underwent orthotopic autotransplantation and received the same antibiotic and selective decontamination regimens as group B. Animals ...

Microbiologia Medica, 2011

Valutazione della riattivazione di HSV1, HHV6, CMV ed EBV in una popolazione di pazienti sottopos... more Valutazione della riattivazione di HSV1, HHV6, CMV ed EBV in una popolazione di pazienti sottoposti a trapianto allogenico di midollo

Journal of Viral Hepatitis, 2014

SummaryA cytoplasmic antigen associated to inosine‐5'‐monophosphatedehydrogenase 2 eliciting ... more SummaryA cytoplasmic antigen associated to inosine‐5'‐monophosphatedehydrogenase 2 eliciting specific antibodies (antirods and rings, RR) has been identified in patients with chronic hepatitis C who were exposed to pegylated interferon (PI) and ribavirin (RBV). The significance of anti‐RR in these patients merits to be investigated. Sera from 88 chronic hepatitis C virus (HCV)‐infected patients undergoing PI‐RBV therapy were analysed for the presence of RR pattern by indirect immunofluorescence on HEp‐2 substrate (Inova Diagnostics, San Diego, CA, USA). Anti‐RR antibodies developed de novo in 32 patients independently of any demographic and virological feature, but with a significant association with cumulative exposure to PI‐RBV (P = 0.0089; chi‐square test). RR pattern was significantly more frequent in relapsers than in patients achieving sustained virological response (56% vs 30%; P = 0.0282, chi‐square test). Anti‐RR titre ranged from 1:80 to 1:1280, but significantly decli...

Microbiologia Medica, 2012

Sieroprevalenza di West Nile Virus (WNV) in una popolazione di donatori di Milano SUMMARY A serop... more Sieroprevalenza di West Nile Virus (WNV) in una popolazione di donatori di Milano SUMMARY A seroprevalence study for anti West Nile virus was carried out among 864 healthy blood donors living in the metropolitan area of Milan by using a commercially available ELISA method. In addition, the performance of a novel ELISA assay for WNV antibodies was assessed. The sero-prevalence rate of WNV antibodies was 0.57% thus showing that WNV is likely circulating also in this up to now unknown area. The overall sensitivity and specificity of the novel ELISA were 99.9% and 45.4%, respectively, well comparable with that of the chosen reference immunoenzimatic method.