Cem Sezer - Academia.edu (original) (raw)

Papers by Cem Sezer

Research paper thumbnail of Large-cell calcifying Sertoli cell tumor of the testis: appearance at sonography

American Journal of Roentgenology, 1994

Research paper thumbnail of The role of High Mobility Group Box 1 (HMGB1) in colorectal cancer

Medical Science Monitor, 2014

Departmental sources Background: HMGB1, the most important member of the high mobility group box ... more Departmental sources Background: HMGB1, the most important member of the high mobility group box protein family, is a nuclear protein with different functions in the cell; it has a role in cancer progression, angiogenesis, invasion, and metastasis development. We studied the expression of HMGB1 and whether it is a prognostic factor in colorectal carcinoma. Material/Methods: The study included 110 cases that were histopathologically diagnosed with colorectal carcinoma from the tissue samples acquired by surgical resection and biopsy in Antalya Education and Research Hospital between 2008 and 2012. HMGB1 expression was examined via immunohistochemical method. Results: HMGB1 expression was evaluated as negative in 32 (44.4%) of the patients and as positive in 40 (55.6%) patients. There was no relation between the HMGB1 expression and sex, age, tumor invasion depth, and histological type. However, a significant relation was detected between the HMGB1 expression and lymph node status, metastasis status, and stage (p:<0.001, p:<0.001, p:<0.001, respectively). Similar results were obtained for the relations between the HMGB1 and histological grade, perineural invasion, lymphovascular invasion, and lymphocytic response (p<0.001, p<0.001, p<0.001, and p<0.001, respectively). Conclusions: The results of our study demonstrate that HMGB1 overexpression has a significant role in tumor progression (especially migration of tumor cells) and tumor ability to metastasize in colorectal cancers; thus, it corroborates the idea that it might be an important prognostic factor.

Research paper thumbnail of Multiple Myeloma: Relationship to Antioxidant Esterases

Medical Principles and Practice, 2014

Objective: To investigate the status of the oxidant/antioxidant balance in patients with multiple... more Objective: To investigate the status of the oxidant/antioxidant balance in patients with multiple myeloma compared to healthy controls. Materials and Methods: This study was conducted on 40 multiple myeloma patients and 40 healthy controls of matched age and sex. Serum total thiol, oxidative stress index (OSI), total oxidant status (TOS), and total antioxidant status (TAS) were measured using colourimetric methods; paraoxonase-1 and arylesterase enzyme activities were also quantified. Results: Serum paraoxonase-1 and arylesterase activities and total thiol levels were significantly lower (p = 0.0001, p = 0.036 and p < 0.0001, respectively), whereas TOS and OSI levels were significantly higher (p < 0.0001 for both parameters) in multiple myeloma patients compared to controls. However, no significant differences in TAS were identified when the two groups were compared. Conclusions: Our findings indicate an impaired oxidative/antioxidative balance in multiple myeloma. We recommen...

Research paper thumbnail of Enhanced HDL-cholesterol-associated anti-oxidant PON-1 activity in prostate cancer patients

The Kaohsiung Journal of Medical Sciences, 2013

Increases in the generation of reactive oxygen species and decreases in antioxidant enzyme activi... more Increases in the generation of reactive oxygen species and decreases in antioxidant enzyme activities with aging have been reported in the prostate, and are also observed in agerelated disorders such as atherosclerosis, Alzheimer's disease, and cataracts. Several studies have demonstrated that proteins are targets for reactive oxidants in cells, and that oxidized proteins accumulate during aging, oxidative stress and in some pathological conditions. However, only a limited number of studies have actually evaluated oxidative damage in relation to HDL-cholesterol-associated antioxidant enzyme activities or have assessed its relationship with prostate cancer. In this study, we examined the effect of HDL-cholesterol-associated antioxidant enzyme activities, paraoxonase1, arylesterase and new oxidative stress parameters (total oxidant status, total antioxidant status [and oxidative stress index]) in newlydiagnosed prostate cancer patients and healthy controls. There were no significant differences in oxidative stress parameters and lipid parameters between prostate cancer patients and controls, however, paraoxonase1 enzyme activity, and non-HDL-cholesterol levels were higher in prostate cancer patients than controls. The results of this study were derived from a small number of subjects, but might represent an important working hypothesis for further research in a larger number of cases to clarify the role of paraoxonase1 overproduction on the prostate and its clinical relevance.

Research paper thumbnail of P130 The densities of T cells, Langerhans cells, and natural killer cells in uninvolved skin of patients with acne vulgaris

Journal of the European Academy of Dermatology and Venereology, 1997

Acne Vulgaris (AV) is a disease which affects the whole pilosebaseous follicle, and results in th... more Acne Vulgaris (AV) is a disease which affects the whole pilosebaseous follicle, and results in the formation of non-inflamed and inflamed lesions. The purpose of the present work was to investigate the inflammatory infiltrating cells in early AV lesions. We examined 25 biopsies, 10 of them were moderate to severe acne cases and 15 of them were healthy control subjects. All specimens evaluated for the density of the CD3+ (total T lymphocyte), CD4+ (helper/inducer T lymphocyte), CD8+ (suppressor/cytotoxic T lymphocyte), CDla+ (Langerhans cells), and CD56+ (Natural Killer cells) in the epidermis and dermis immunocytochemically. Only the CD4+ cells were found to be statistically elevated in dermis of patients with AV when compared to healthy subjects (p < 0.05).

Research paper thumbnail of P126 The densities of T cells, Langerhans cells, and natural killer cells in uninvolved skin of patients with Acne Vulgaris

Journal of the European Academy of Dermatology and Venereology, 1997

St83 treating severe forms of acne. 116 patients (50 females, 66 males were treated for different... more St83 treating severe forms of acne. 116 patients (50 females, 66 males were treated for different forms of acne. All the patients had a history of continuous systemic antibiotics treatment varying from 2 months to 2 years. The patients suffered from their acne for 7 months to 28 years. All the female patients (15-32 years of age were administered contraceptives. 47 of them used Diane 35, and 3 were given other medicaments for their intolerance of Diane 35. The initial dose was 0.5 mg/kg bw in 92 patients, while in 12 patients the dose was 1 mg/kg. and in 2 other-0.2-03 mg/kg. The dosage was modified during treatment. Treatment was discontinued in 5 patients for the lack of results and/or side effects. Other patients were assymptomatic after treatment for 3-7 months. A two-year symptomless period was observed in 61 patients, relapses were noted in the period of 7-12 months after completion. In 24 patients no relevant observations were possible. The list of side effects comprised dryness of lips and mucosa in all the patients, hair loss in some of the patients, and sporadic epistaxis. I P126 The densities of T cells, Langerhans cells, and natural killer cells in uninvolved skin of patients with Acne Vulgaris

Research paper thumbnail of Nasal folliculosebaceous hamartoma with vascular–mesenchymal overgrowth in an infant

Journal of Cranio-Maxillofacial Surgery, 2013

The nasal hamartoma is a rare non-neoplastic malformation or inborn error of tissue development, ... more The nasal hamartoma is a rare non-neoplastic malformation or inborn error of tissue development, characterized by a mix of several tissue types. Folliculosebaceous cystic hamartoma is a distinct type of cutaneous hamartoma. We present a 3-month-old male infant who had nasal obstruction and who had a diagnosis of nasal folliculosebaceous cystic hamartoma after surgery.

Research paper thumbnail of The potential role of inducible nitric oxide synthase (iNOS) activity in the testicular dysfunction associated with varicocele: An experimental study

International Urology and Nephrology, 2004

Nitric oxide (NO) has been reported to be increased in the spermatic veins of men affected by var... more Nitric oxide (NO) has been reported to be increased in the spermatic veins of men affected by varicocele. The aim of the present study was to determine whether iNOS (inducible nitric oxide synthase) has a role in testicular dysfunction associated with varicocele, immunohistochemistry analyze was used to study iNOS activity in testis of adolescent rats with experimental left varicoceles. Rats were randomly divided into three groups. The first group consisted of rats undergoing partial ligation of left renal vein (n:12). The second group consisted of rats undergoing a sham operation (n:6) and, the third group referred to as control rats (n:7). Immunohistochemistry slides were evaluated by counting the number of positive cells and expressed as percents (% iNOS activity). We found that iNOS was predominantly expressed in the cytoplasm of Leydig cells in each group and only a small amount of iNOS was expressed in Sertoli cells. There were significant differences in % iNOS activity between both testes of varicocele group and both of testes control group (p < 0.01), but no significant differences were noted between other groups (p > 0.05). Because of iNOS activity was markedly increased in the Leydig cells of varicocele bearing rats, we suggest that iNOS activity may play a role in the testicular dysfunction associated with varicocele during adolescence.

Research paper thumbnail of Keratocyte apoptosis and corneal antioxidant enzyme activities after refractive corneal surgery

Eye, 2002

Purpose Refractive corneal surgery induces keratocyte apoptosis and generates reactive oxygen rad... more Purpose Refractive corneal surgery induces keratocyte apoptosis and generates reactive oxygen radicals (ROS) in the cornea. The purpose of the present study is to evaluate the correlation between keratocyte apoptosis and corneal antioxidant enzyme activities after different refractive surgical procedures in rabbits. Methods Rabbits were divided into six groups. All groups were compared with the control group (Group 1), after epithelial scraping (Group 2), epithelial scrape and photorefractive keratectomy (PRK) (traditional PRK: Group 3), transepithelial PRK (Group 4), creation of a corneal flap with microkeratome (Group 5) and laserassisted in situ keratomileusis (LASIK, Group 6). Terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nickend labelling assay (to detect DNA fragmentation in situ) and light microscopy were used to detect apoptosis in rabbit eyes. Glutathione peroxidase (Gpx) and superoxide dismutase (SOD) activities of the corneal tissues were measured with spectrophotometric methods. Results Corneal Gpx and SOD activities decreased significantly in all groups when compared with the control group (PϽ0.05) and groups 2, 3 and 6 showed a significantly higher amount of keratocyte apoptosis (PϽ0.05). Not only a negative correlation was observed between corneal SOD activity and keratocyte apoptosis (cc: ؊0.3648) but Gpx activity also showed negative correlation with keratocyte apoptosis (cc: ؊0.3587). Conclusion The present study illustrates the negative correlation between keratocyte apoptosis and corneal antioxidant enzyme activities. This finding suggests that ROS may be partly responsible for keratocyte apoptosis after refractive surgery.

Research paper thumbnail of Effect of mechanical vs dilute ethanol epithelial removal on keratocyte apoptosis and polymorphonuclear leukocyte migration

Eye, 2002

Purpose To investigate keratocyte apoptosis and polymorphonuclear (PMN) cell infiltration to the ... more Purpose To investigate keratocyte apoptosis and polymorphonuclear (PMN) cell infiltration to the corneal stroma after mechanical epithelial scraping and chemical de-epithelialization with 18% ethanol solution. Methods Twelve New Zealand Albino rabbits (24 eyes) were randomly divided into three groups. Group A was the control group with no epithelial removal. Group B underwent a 7.5-mm mechanical epithelial removal with a blunt spatula. Group C underwent 7.5-mm chemical deepithelialization with 18% ethanol-balanced salt solution. Corneas were stained with terminal deoxyribonucleotidyl transferasemediated dUTP-digoxigenin nick-end labeling (TUNEL) assay after 24 h. Only nuclear staining in keratocytes was counted. Polymorphonuclear (PMN) leukocyte densities were also assessed by light microscopy. Results Mechanical de-epithelialization (group B) and chemical de-epithelialization with 18% ethanol (group C) showed no difference in keratocyte apoptosis compared with the control group. There was also no difference between groups B and C. Group B showed no difference in PMN leukocyte counts compared with the control group. But the number of PMN leukocytes observed in group C was significantly higher than those encountered in the corneas of the control group (P Ͻ 0.05) and group B (P Ͻ 0.05). Conclusions Dilute alcohol induces more PMN cell infiltration when compared with mechanical de-epithelialization although there is no difference in the apoptosis rates.

Research paper thumbnail of Is Qualitative Histological Interpretation of the Prostatic Biopsy Specimen Helpful in Predicting Clinical Outcome of Terazosin Therapy?

Research paper thumbnail of Mekanik Epitel Debridmanı Sonrası Topikal Vitamin-E ve Askorbik Asid Uygulamasının Keratosit Apoptozisine Etkisinin Tavşan Gözünde İncelenmesi

Turkiye Klinikleri J Ophthalmol, 2004

Research paper thumbnail of Effects of topical vitamin E on corneal superoxide dismutase, glutathione peroxidase activities and polymorphonuclear leucocyte infiltration after photorefractive keratectomy

Acta Ophthalmologica Scandinavica, 2003

Photorefractive keratectomy (PRK) induces free radical formation and polymorphonuclear (PMN) cell... more Photorefractive keratectomy (PRK) induces free radical formation and polymorphonuclear (PMN) cell infiltration in the cornea. Vitamin E is a free radical scavenger and protects the cells from reactive oxygen species. We investigated the effects of topical vitamin E on corneal PMN cell infiltration and corneal antioxidant enzyme activities after PRK. We studied four groups, each consisting of seven eyes. Group 1 were control eyes. In group 2 the corneal epithelium was removed by a blunt spatula (epithelial scrape). In group 3, corneal photoablation (59 micro m, 5 dioptres) was performed after epithelial removal (traditional PRK). In group 4 we tested the effects of topical Vitamin E after traditional PRK. Corneal tissues were removed and studied with enzymatic analysis (measurement of corneal superoxide dismutase and glutathione peroxidase activities) and histologically. Stromal PMN leucocyte counts were significantly higher after mechanical epithelial removal and traditional PRK (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Corneal superoxide dismutase and glutathione peroxidase activities decreased significantly after mechanical epithelial removal and traditional PRK (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). In group 4, treated with vitamin E, corneal superoxide dismutase activity did not differ significantly from that in the medically non-treated groups, nor did corneal PMN cell infiltration after traditional PRK. The reduction of corneal glutathione peroxidase activity after PRK was reduced significantly after topical vitamin E treatment. Topical vitamin E treatment may be useful for reducing the harmful effects of reactive oxygen radical after epithelial scraping and PRK in that it increases corneal glutathione peroxidase activity.

Research paper thumbnail of Analysis of High Risk Hpv Subtypes Associated with Cervical Intraepithelial Neoplasia: A Single Centre Retrospective Study in the Mediterranean Region of Turkey / Servikal İntraepiteliyal Neoplazi ile İlişkili Yüksek Risk Hpv Alt Tiplerinin Analizi: Türkiye’nin Akdeniz Bölgesinde Tek Merkezli Ret...

Türk Patoloji Dergisi, 2014

Various studies on the Human Papilloma Virus (HPV) types seen in our country have been conducted ... more Various studies on the Human Papilloma Virus (HPV) types seen in our country have been conducted previously but they have been inadequate due to the insufficient number of patients as well as not representing a wide geographical area. Recently Dursun P. et al. conducted a multi-centered study on a large series of 6388 cases related to this issue and determined HPV positivity in 25% of the cases. In addition, this study investigated the incidence of HPV in patients with abnormal and normal cytology and the relationship between abnormal cytological findings and HPV types (1). HPV is known to play a role in precancerous and invasive lesions of the cervix and the incidence of these lesions is gradually increasing (2-6). We conducted an HPV subtype study in our region by investigating the presence of cervical precancerous lesions in cases examined for high-risk HPV DNA with the PCR method in the cervicovaginal cytology materials and found to be positive.

Research paper thumbnail of Pleomorphic adenoma of an unusual size in the deep lobe of the parotid gland

International Journal of Pediatric Otorhinolaryngology, 1995

Tumors of the salivary glands in children are not as common as in adults and they are mostly beni... more Tumors of the salivary glands in children are not as common as in adults and they are mostly benign. The most common benign epithelial neoplasm seen in children is pleomorphic adenoma. As is usually the case in the adult population, it is usually found in the superficial lobe of the parotid gland. Its localization in the deep lobe is rare. Pleomorphic adenomas of the deep lobe present with a neck mass and when it is large, it may have an intraoral extension as well. It may also assume a dumb-bell shape as it enlarges and may be observed on CT or MRI scans as a prestyloid mass. The choice of treatment depends on the localization and the size of the tumor. The method can be transoral, cervical, transmandibular, transparotid, retromandibular fossa or a combination of these. A child with an unusually large pleomorphic adenoma of the deep lobe of the parotid gland is presented in this study. The deep lobe tumor was totally removed using a combination of cervical and transmandibular approaches.

Research paper thumbnail of Chondroid Syringoma

Dermatologic Surgery, 2003

ABSTRACT BACKGROUND: Chondroid syringoma or mixed tumor of the skin is a rare subcutaneous tumor ... more ABSTRACT BACKGROUND: Chondroid syringoma or mixed tumor of the skin is a rare subcutaneous tumor that may be confused with various skin lesions. OBJECTIVE: To elucidate the incidence of condroid syringomas among skin lesions that were excised under local anesthesia. METHODS: The histopathologic diagnosis of 16,200 skin lesions that had been operated between 1986 and 2002 were retrospectively evaluated. The cases with condroid syringoma were histopathologically re-examined, and confirmed cases were further analyzed for preoperative diagnosis, age, gender, and lesion location. RESULTS: Sixteen patients were found to have histopathologic diagnosis of chondroid syringoma constituting 0.098% of the excised skin lesions in this series. All of these 16 cases were misdiagnosed preoperatively. The typical presentation was a solitary skin lesion located in the head and neck region in a middle-aged male patient. CONCLUSION: In the evaluation of a middle-aged male patient with a small subcutaneous nodule in the head and neck region, chondroid syringoma should be also taken into consideration for differential diagnosis. For such a lesion, excisional biopsy without destroying aesthetic and functional structures is the preferred diagnostic approach.

Research paper thumbnail of Management of thyroglossal duct cysts in children

Pediatrics International, 2004

Background : The recurrence rate of thyroglossal duct cysts removed by Sistrunk or other procedur... more Background : The recurrence rate of thyroglossal duct cysts removed by Sistrunk or other procedures is 4% and 50%, respectively. The aim of the present study was to explain the reasons for recurrence and misdiagnosis. Methods : Twenty-seven children underwent surgery for thyroglossal cysts and fistulas during 1989-2000. Age, sex, length of history, presentation, preoperative investigations, operative findings, histopathology of the lesion and length of excised hyoid bone, postoperative complications and length of follow-up were recorded. Statistical analysis was performed using Fisher's exact test with a significance level of P < 0.05. Results : Twenty-three patients were primary referrals and four were secondary referrals having had previous surgery with misdiagnosis. The recurrence rate after a Sistrunk procedure was similar to the rate indicated in the literature (3.7%); however, no related special features could be identified such as inflammation (n = 12), perforation at surgery (n = 7), presentation with fistula (n = 4) or previous drainage of abscess (n = 5) (P > 0.05). Conclusion : Misdiagnosis is the most common cause of inadequate and inappropriate surgery, leading to recurrence of the lesion. The authors recommend a Sistrunk procedure for all cases of suspected thyroglossal duct cysts.

Research paper thumbnail of Omentum prevents intestinal adhesions to mesh graft in abdominal infections and serosal defects

Surgical Endoscopy, 2006

Background: Many studies have evaluated the use of grafts in the reconstruction of abdominal wall... more Background: Many studies have evaluated the use of grafts in the reconstruction of abdominal wall defects. In this study, the effects resulting from the presence or absence of the omentum were evaluated in the setting of infection or serosal defects in the formation of adhesions in abdominal closures using mesh grafts. Methods: For this study, 60 Wistar albino rats were divided into six groups. A circular 3.79-cm 2 fascioperitoneal defect was created. After group-specific procedures, defects were reconstructed using polypropylene mesh grafts. In group C (control group), only a mesh graft recontruction was performed, whereas group O (O for omentectomy) underwent an omentectomy plus mesh closure. In group SD (serosal defect group), the cecum was abrased with a brush before mesh closure. Group SDO underwent cecal abrasion plus an omentectomy. In group I (infection group), the intraabdominal space was filled with 1 ml of solution containing 100,000 colony-forming units (CFUs) of Escherichia coli per milliliter. Group IO received the same same amount of E. coli solution plus an omentectomy before mesh closure. After 28 days, the groups were evaluated by intraabdominal and blood cultures, grading of intraabdominal adhesions, graft-organ adhesions, proportion of adhesions to graft size, and histopathologic studies. The results were statistically evaluated using one-way variant analysis and ScheffeÕs and FisherÕs definite chisquare tests. Results: For the groups in which the greater omentum was preserved, intestinal adhesions to the graft surface were less frequently observed, especially in cases with intraabdominal infections and serosal defects (p < 0.05). Conclusions: Preservation of the greater omentum reduces the formation of intestinal adhesions, especially in cases with underlying infections and serosal defects in abdominal closures using mesh grafts. This could be beneficial in related clinical situations in lowering the rate of intestinal fistulas, erosions, and obstructions that can be attributed to the formation of adhesions.

Research paper thumbnail of Effect of absorbent pads containing oregano essential oil on the shelf life extension of overwrap packed chicken drumsticks stored at four degrees Celsius

Poultry Science, 2009

The addition of sachets or pads containing volatile antimicrobial agents into packages has been t... more The addition of sachets or pads containing volatile antimicrobial agents into packages has been the most successful commercial application of antimicrobials to packaging. In this study, the effect of oregano (Origanum onites) essential oil on the extension of shelf life of overwrap packed fresh chicken drumsticks was investigated. Meat exudate absorbent pads were sprayed with 5 mL of oregano essential oil at a concentration of 1.5% in distillate water. Sampling was carried out at 0, 3, 5, and 7 d of the refrigerated storage. Total viable count, psychrotrophs, pseudomonads, members of the family Enterobacteriaceae, yeasts, and lactic acid bacteria were enumerated. Physicochemical analysis and sensorial evaluation were also conducted. The shelf life of fresh chicken drumsticks was approximately 3 d. Oregano essential oil extended product shelf life by approximately 2 d. Thus, incorporation of essential oils to absorbent pads may have supplementary applications in food packaging.

Research paper thumbnail of Different types of neural cell death in the cerebellum of the ataxia and male sterility (AMS) mutant mouse

Pathology International, 2006

To investigate the mechanisms(s) of age-dependent atrophy of the cerebellum of the ataxia and mal... more To investigate the mechanisms(s) of age-dependent atrophy of the cerebellum of the ataxia and male sterility (AMS) mouse at young age, the morphological changes were evaluated and the nature of neural cell death was examined. Dying Purkinje cells lacked characters of classical apoptosis except for light microscopic morphology, but their death was considered to be autonomous death triggered by the direct effect of ams mutation, because of the acute and near-complete disappearance and particular change of the cytoplasm. In contrast, in the granular layer, typical apoptotic bodies were recognized by electron microscopy, and substantial numbers of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end-labeling (TUNEL)-positive cells and activated caspase-3-positive cells were observed. Granule cell death was considered to be target-related apoptosis induced after post-synaptic Purkinje cell death, because the age-dependent changes in TUNEL-positive cell counts followed that of Purkinje cell loss and the peak value was still noted 1 week after total loss of Purkinje cells. These results indicate that both total and partial losses of Purkinje cells and granule cells, respectively, contributed to the atrophy of the AMS cerebellum. Furthermore, different types of neuronal death were recognized; the granule cell death was apoptotic while Purkinje cell death was different from that of classical apoptosis.

Research paper thumbnail of Large-cell calcifying Sertoli cell tumor of the testis: appearance at sonography

American Journal of Roentgenology, 1994

Research paper thumbnail of The role of High Mobility Group Box 1 (HMGB1) in colorectal cancer

Medical Science Monitor, 2014

Departmental sources Background: HMGB1, the most important member of the high mobility group box ... more Departmental sources Background: HMGB1, the most important member of the high mobility group box protein family, is a nuclear protein with different functions in the cell; it has a role in cancer progression, angiogenesis, invasion, and metastasis development. We studied the expression of HMGB1 and whether it is a prognostic factor in colorectal carcinoma. Material/Methods: The study included 110 cases that were histopathologically diagnosed with colorectal carcinoma from the tissue samples acquired by surgical resection and biopsy in Antalya Education and Research Hospital between 2008 and 2012. HMGB1 expression was examined via immunohistochemical method. Results: HMGB1 expression was evaluated as negative in 32 (44.4%) of the patients and as positive in 40 (55.6%) patients. There was no relation between the HMGB1 expression and sex, age, tumor invasion depth, and histological type. However, a significant relation was detected between the HMGB1 expression and lymph node status, metastasis status, and stage (p:<0.001, p:<0.001, p:<0.001, respectively). Similar results were obtained for the relations between the HMGB1 and histological grade, perineural invasion, lymphovascular invasion, and lymphocytic response (p<0.001, p<0.001, p<0.001, and p<0.001, respectively). Conclusions: The results of our study demonstrate that HMGB1 overexpression has a significant role in tumor progression (especially migration of tumor cells) and tumor ability to metastasize in colorectal cancers; thus, it corroborates the idea that it might be an important prognostic factor.

Research paper thumbnail of Multiple Myeloma: Relationship to Antioxidant Esterases

Medical Principles and Practice, 2014

Objective: To investigate the status of the oxidant/antioxidant balance in patients with multiple... more Objective: To investigate the status of the oxidant/antioxidant balance in patients with multiple myeloma compared to healthy controls. Materials and Methods: This study was conducted on 40 multiple myeloma patients and 40 healthy controls of matched age and sex. Serum total thiol, oxidative stress index (OSI), total oxidant status (TOS), and total antioxidant status (TAS) were measured using colourimetric methods; paraoxonase-1 and arylesterase enzyme activities were also quantified. Results: Serum paraoxonase-1 and arylesterase activities and total thiol levels were significantly lower (p = 0.0001, p = 0.036 and p < 0.0001, respectively), whereas TOS and OSI levels were significantly higher (p < 0.0001 for both parameters) in multiple myeloma patients compared to controls. However, no significant differences in TAS were identified when the two groups were compared. Conclusions: Our findings indicate an impaired oxidative/antioxidative balance in multiple myeloma. We recommen...

Research paper thumbnail of Enhanced HDL-cholesterol-associated anti-oxidant PON-1 activity in prostate cancer patients

The Kaohsiung Journal of Medical Sciences, 2013

Increases in the generation of reactive oxygen species and decreases in antioxidant enzyme activi... more Increases in the generation of reactive oxygen species and decreases in antioxidant enzyme activities with aging have been reported in the prostate, and are also observed in agerelated disorders such as atherosclerosis, Alzheimer's disease, and cataracts. Several studies have demonstrated that proteins are targets for reactive oxidants in cells, and that oxidized proteins accumulate during aging, oxidative stress and in some pathological conditions. However, only a limited number of studies have actually evaluated oxidative damage in relation to HDL-cholesterol-associated antioxidant enzyme activities or have assessed its relationship with prostate cancer. In this study, we examined the effect of HDL-cholesterol-associated antioxidant enzyme activities, paraoxonase1, arylesterase and new oxidative stress parameters (total oxidant status, total antioxidant status [and oxidative stress index]) in newlydiagnosed prostate cancer patients and healthy controls. There were no significant differences in oxidative stress parameters and lipid parameters between prostate cancer patients and controls, however, paraoxonase1 enzyme activity, and non-HDL-cholesterol levels were higher in prostate cancer patients than controls. The results of this study were derived from a small number of subjects, but might represent an important working hypothesis for further research in a larger number of cases to clarify the role of paraoxonase1 overproduction on the prostate and its clinical relevance.

Research paper thumbnail of P130 The densities of T cells, Langerhans cells, and natural killer cells in uninvolved skin of patients with acne vulgaris

Journal of the European Academy of Dermatology and Venereology, 1997

Acne Vulgaris (AV) is a disease which affects the whole pilosebaseous follicle, and results in th... more Acne Vulgaris (AV) is a disease which affects the whole pilosebaseous follicle, and results in the formation of non-inflamed and inflamed lesions. The purpose of the present work was to investigate the inflammatory infiltrating cells in early AV lesions. We examined 25 biopsies, 10 of them were moderate to severe acne cases and 15 of them were healthy control subjects. All specimens evaluated for the density of the CD3+ (total T lymphocyte), CD4+ (helper/inducer T lymphocyte), CD8+ (suppressor/cytotoxic T lymphocyte), CDla+ (Langerhans cells), and CD56+ (Natural Killer cells) in the epidermis and dermis immunocytochemically. Only the CD4+ cells were found to be statistically elevated in dermis of patients with AV when compared to healthy subjects (p < 0.05).

Research paper thumbnail of P126 The densities of T cells, Langerhans cells, and natural killer cells in uninvolved skin of patients with Acne Vulgaris

Journal of the European Academy of Dermatology and Venereology, 1997

St83 treating severe forms of acne. 116 patients (50 females, 66 males were treated for different... more St83 treating severe forms of acne. 116 patients (50 females, 66 males were treated for different forms of acne. All the patients had a history of continuous systemic antibiotics treatment varying from 2 months to 2 years. The patients suffered from their acne for 7 months to 28 years. All the female patients (15-32 years of age were administered contraceptives. 47 of them used Diane 35, and 3 were given other medicaments for their intolerance of Diane 35. The initial dose was 0.5 mg/kg bw in 92 patients, while in 12 patients the dose was 1 mg/kg. and in 2 other-0.2-03 mg/kg. The dosage was modified during treatment. Treatment was discontinued in 5 patients for the lack of results and/or side effects. Other patients were assymptomatic after treatment for 3-7 months. A two-year symptomless period was observed in 61 patients, relapses were noted in the period of 7-12 months after completion. In 24 patients no relevant observations were possible. The list of side effects comprised dryness of lips and mucosa in all the patients, hair loss in some of the patients, and sporadic epistaxis. I P126 The densities of T cells, Langerhans cells, and natural killer cells in uninvolved skin of patients with Acne Vulgaris

Research paper thumbnail of Nasal folliculosebaceous hamartoma with vascular–mesenchymal overgrowth in an infant

Journal of Cranio-Maxillofacial Surgery, 2013

The nasal hamartoma is a rare non-neoplastic malformation or inborn error of tissue development, ... more The nasal hamartoma is a rare non-neoplastic malformation or inborn error of tissue development, characterized by a mix of several tissue types. Folliculosebaceous cystic hamartoma is a distinct type of cutaneous hamartoma. We present a 3-month-old male infant who had nasal obstruction and who had a diagnosis of nasal folliculosebaceous cystic hamartoma after surgery.

Research paper thumbnail of The potential role of inducible nitric oxide synthase (iNOS) activity in the testicular dysfunction associated with varicocele: An experimental study

International Urology and Nephrology, 2004

Nitric oxide (NO) has been reported to be increased in the spermatic veins of men affected by var... more Nitric oxide (NO) has been reported to be increased in the spermatic veins of men affected by varicocele. The aim of the present study was to determine whether iNOS (inducible nitric oxide synthase) has a role in testicular dysfunction associated with varicocele, immunohistochemistry analyze was used to study iNOS activity in testis of adolescent rats with experimental left varicoceles. Rats were randomly divided into three groups. The first group consisted of rats undergoing partial ligation of left renal vein (n:12). The second group consisted of rats undergoing a sham operation (n:6) and, the third group referred to as control rats (n:7). Immunohistochemistry slides were evaluated by counting the number of positive cells and expressed as percents (% iNOS activity). We found that iNOS was predominantly expressed in the cytoplasm of Leydig cells in each group and only a small amount of iNOS was expressed in Sertoli cells. There were significant differences in % iNOS activity between both testes of varicocele group and both of testes control group (p < 0.01), but no significant differences were noted between other groups (p > 0.05). Because of iNOS activity was markedly increased in the Leydig cells of varicocele bearing rats, we suggest that iNOS activity may play a role in the testicular dysfunction associated with varicocele during adolescence.

Research paper thumbnail of Keratocyte apoptosis and corneal antioxidant enzyme activities after refractive corneal surgery

Eye, 2002

Purpose Refractive corneal surgery induces keratocyte apoptosis and generates reactive oxygen rad... more Purpose Refractive corneal surgery induces keratocyte apoptosis and generates reactive oxygen radicals (ROS) in the cornea. The purpose of the present study is to evaluate the correlation between keratocyte apoptosis and corneal antioxidant enzyme activities after different refractive surgical procedures in rabbits. Methods Rabbits were divided into six groups. All groups were compared with the control group (Group 1), after epithelial scraping (Group 2), epithelial scrape and photorefractive keratectomy (PRK) (traditional PRK: Group 3), transepithelial PRK (Group 4), creation of a corneal flap with microkeratome (Group 5) and laserassisted in situ keratomileusis (LASIK, Group 6). Terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nickend labelling assay (to detect DNA fragmentation in situ) and light microscopy were used to detect apoptosis in rabbit eyes. Glutathione peroxidase (Gpx) and superoxide dismutase (SOD) activities of the corneal tissues were measured with spectrophotometric methods. Results Corneal Gpx and SOD activities decreased significantly in all groups when compared with the control group (PϽ0.05) and groups 2, 3 and 6 showed a significantly higher amount of keratocyte apoptosis (PϽ0.05). Not only a negative correlation was observed between corneal SOD activity and keratocyte apoptosis (cc: ؊0.3648) but Gpx activity also showed negative correlation with keratocyte apoptosis (cc: ؊0.3587). Conclusion The present study illustrates the negative correlation between keratocyte apoptosis and corneal antioxidant enzyme activities. This finding suggests that ROS may be partly responsible for keratocyte apoptosis after refractive surgery.

Research paper thumbnail of Effect of mechanical vs dilute ethanol epithelial removal on keratocyte apoptosis and polymorphonuclear leukocyte migration

Eye, 2002

Purpose To investigate keratocyte apoptosis and polymorphonuclear (PMN) cell infiltration to the ... more Purpose To investigate keratocyte apoptosis and polymorphonuclear (PMN) cell infiltration to the corneal stroma after mechanical epithelial scraping and chemical de-epithelialization with 18% ethanol solution. Methods Twelve New Zealand Albino rabbits (24 eyes) were randomly divided into three groups. Group A was the control group with no epithelial removal. Group B underwent a 7.5-mm mechanical epithelial removal with a blunt spatula. Group C underwent 7.5-mm chemical deepithelialization with 18% ethanol-balanced salt solution. Corneas were stained with terminal deoxyribonucleotidyl transferasemediated dUTP-digoxigenin nick-end labeling (TUNEL) assay after 24 h. Only nuclear staining in keratocytes was counted. Polymorphonuclear (PMN) leukocyte densities were also assessed by light microscopy. Results Mechanical de-epithelialization (group B) and chemical de-epithelialization with 18% ethanol (group C) showed no difference in keratocyte apoptosis compared with the control group. There was also no difference between groups B and C. Group B showed no difference in PMN leukocyte counts compared with the control group. But the number of PMN leukocytes observed in group C was significantly higher than those encountered in the corneas of the control group (P Ͻ 0.05) and group B (P Ͻ 0.05). Conclusions Dilute alcohol induces more PMN cell infiltration when compared with mechanical de-epithelialization although there is no difference in the apoptosis rates.

Research paper thumbnail of Is Qualitative Histological Interpretation of the Prostatic Biopsy Specimen Helpful in Predicting Clinical Outcome of Terazosin Therapy?

Research paper thumbnail of Mekanik Epitel Debridmanı Sonrası Topikal Vitamin-E ve Askorbik Asid Uygulamasının Keratosit Apoptozisine Etkisinin Tavşan Gözünde İncelenmesi

Turkiye Klinikleri J Ophthalmol, 2004

Research paper thumbnail of Effects of topical vitamin E on corneal superoxide dismutase, glutathione peroxidase activities and polymorphonuclear leucocyte infiltration after photorefractive keratectomy

Acta Ophthalmologica Scandinavica, 2003

Photorefractive keratectomy (PRK) induces free radical formation and polymorphonuclear (PMN) cell... more Photorefractive keratectomy (PRK) induces free radical formation and polymorphonuclear (PMN) cell infiltration in the cornea. Vitamin E is a free radical scavenger and protects the cells from reactive oxygen species. We investigated the effects of topical vitamin E on corneal PMN cell infiltration and corneal antioxidant enzyme activities after PRK. We studied four groups, each consisting of seven eyes. Group 1 were control eyes. In group 2 the corneal epithelium was removed by a blunt spatula (epithelial scrape). In group 3, corneal photoablation (59 micro m, 5 dioptres) was performed after epithelial removal (traditional PRK). In group 4 we tested the effects of topical Vitamin E after traditional PRK. Corneal tissues were removed and studied with enzymatic analysis (measurement of corneal superoxide dismutase and glutathione peroxidase activities) and histologically. Stromal PMN leucocyte counts were significantly higher after mechanical epithelial removal and traditional PRK (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). Corneal superoxide dismutase and glutathione peroxidase activities decreased significantly after mechanical epithelial removal and traditional PRK (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). In group 4, treated with vitamin E, corneal superoxide dismutase activity did not differ significantly from that in the medically non-treated groups, nor did corneal PMN cell infiltration after traditional PRK. The reduction of corneal glutathione peroxidase activity after PRK was reduced significantly after topical vitamin E treatment. Topical vitamin E treatment may be useful for reducing the harmful effects of reactive oxygen radical after epithelial scraping and PRK in that it increases corneal glutathione peroxidase activity.

Research paper thumbnail of Analysis of High Risk Hpv Subtypes Associated with Cervical Intraepithelial Neoplasia: A Single Centre Retrospective Study in the Mediterranean Region of Turkey / Servikal İntraepiteliyal Neoplazi ile İlişkili Yüksek Risk Hpv Alt Tiplerinin Analizi: Türkiye’nin Akdeniz Bölgesinde Tek Merkezli Ret...

Türk Patoloji Dergisi, 2014

Various studies on the Human Papilloma Virus (HPV) types seen in our country have been conducted ... more Various studies on the Human Papilloma Virus (HPV) types seen in our country have been conducted previously but they have been inadequate due to the insufficient number of patients as well as not representing a wide geographical area. Recently Dursun P. et al. conducted a multi-centered study on a large series of 6388 cases related to this issue and determined HPV positivity in 25% of the cases. In addition, this study investigated the incidence of HPV in patients with abnormal and normal cytology and the relationship between abnormal cytological findings and HPV types (1). HPV is known to play a role in precancerous and invasive lesions of the cervix and the incidence of these lesions is gradually increasing (2-6). We conducted an HPV subtype study in our region by investigating the presence of cervical precancerous lesions in cases examined for high-risk HPV DNA with the PCR method in the cervicovaginal cytology materials and found to be positive.

Research paper thumbnail of Pleomorphic adenoma of an unusual size in the deep lobe of the parotid gland

International Journal of Pediatric Otorhinolaryngology, 1995

Tumors of the salivary glands in children are not as common as in adults and they are mostly beni... more Tumors of the salivary glands in children are not as common as in adults and they are mostly benign. The most common benign epithelial neoplasm seen in children is pleomorphic adenoma. As is usually the case in the adult population, it is usually found in the superficial lobe of the parotid gland. Its localization in the deep lobe is rare. Pleomorphic adenomas of the deep lobe present with a neck mass and when it is large, it may have an intraoral extension as well. It may also assume a dumb-bell shape as it enlarges and may be observed on CT or MRI scans as a prestyloid mass. The choice of treatment depends on the localization and the size of the tumor. The method can be transoral, cervical, transmandibular, transparotid, retromandibular fossa or a combination of these. A child with an unusually large pleomorphic adenoma of the deep lobe of the parotid gland is presented in this study. The deep lobe tumor was totally removed using a combination of cervical and transmandibular approaches.

Research paper thumbnail of Chondroid Syringoma

Dermatologic Surgery, 2003

ABSTRACT BACKGROUND: Chondroid syringoma or mixed tumor of the skin is a rare subcutaneous tumor ... more ABSTRACT BACKGROUND: Chondroid syringoma or mixed tumor of the skin is a rare subcutaneous tumor that may be confused with various skin lesions. OBJECTIVE: To elucidate the incidence of condroid syringomas among skin lesions that were excised under local anesthesia. METHODS: The histopathologic diagnosis of 16,200 skin lesions that had been operated between 1986 and 2002 were retrospectively evaluated. The cases with condroid syringoma were histopathologically re-examined, and confirmed cases were further analyzed for preoperative diagnosis, age, gender, and lesion location. RESULTS: Sixteen patients were found to have histopathologic diagnosis of chondroid syringoma constituting 0.098% of the excised skin lesions in this series. All of these 16 cases were misdiagnosed preoperatively. The typical presentation was a solitary skin lesion located in the head and neck region in a middle-aged male patient. CONCLUSION: In the evaluation of a middle-aged male patient with a small subcutaneous nodule in the head and neck region, chondroid syringoma should be also taken into consideration for differential diagnosis. For such a lesion, excisional biopsy without destroying aesthetic and functional structures is the preferred diagnostic approach.

Research paper thumbnail of Management of thyroglossal duct cysts in children

Pediatrics International, 2004

Background : The recurrence rate of thyroglossal duct cysts removed by Sistrunk or other procedur... more Background : The recurrence rate of thyroglossal duct cysts removed by Sistrunk or other procedures is 4% and 50%, respectively. The aim of the present study was to explain the reasons for recurrence and misdiagnosis. Methods : Twenty-seven children underwent surgery for thyroglossal cysts and fistulas during 1989-2000. Age, sex, length of history, presentation, preoperative investigations, operative findings, histopathology of the lesion and length of excised hyoid bone, postoperative complications and length of follow-up were recorded. Statistical analysis was performed using Fisher's exact test with a significance level of P < 0.05. Results : Twenty-three patients were primary referrals and four were secondary referrals having had previous surgery with misdiagnosis. The recurrence rate after a Sistrunk procedure was similar to the rate indicated in the literature (3.7%); however, no related special features could be identified such as inflammation (n = 12), perforation at surgery (n = 7), presentation with fistula (n = 4) or previous drainage of abscess (n = 5) (P > 0.05). Conclusion : Misdiagnosis is the most common cause of inadequate and inappropriate surgery, leading to recurrence of the lesion. The authors recommend a Sistrunk procedure for all cases of suspected thyroglossal duct cysts.

Research paper thumbnail of Omentum prevents intestinal adhesions to mesh graft in abdominal infections and serosal defects

Surgical Endoscopy, 2006

Background: Many studies have evaluated the use of grafts in the reconstruction of abdominal wall... more Background: Many studies have evaluated the use of grafts in the reconstruction of abdominal wall defects. In this study, the effects resulting from the presence or absence of the omentum were evaluated in the setting of infection or serosal defects in the formation of adhesions in abdominal closures using mesh grafts. Methods: For this study, 60 Wistar albino rats were divided into six groups. A circular 3.79-cm 2 fascioperitoneal defect was created. After group-specific procedures, defects were reconstructed using polypropylene mesh grafts. In group C (control group), only a mesh graft recontruction was performed, whereas group O (O for omentectomy) underwent an omentectomy plus mesh closure. In group SD (serosal defect group), the cecum was abrased with a brush before mesh closure. Group SDO underwent cecal abrasion plus an omentectomy. In group I (infection group), the intraabdominal space was filled with 1 ml of solution containing 100,000 colony-forming units (CFUs) of Escherichia coli per milliliter. Group IO received the same same amount of E. coli solution plus an omentectomy before mesh closure. After 28 days, the groups were evaluated by intraabdominal and blood cultures, grading of intraabdominal adhesions, graft-organ adhesions, proportion of adhesions to graft size, and histopathologic studies. The results were statistically evaluated using one-way variant analysis and ScheffeÕs and FisherÕs definite chisquare tests. Results: For the groups in which the greater omentum was preserved, intestinal adhesions to the graft surface were less frequently observed, especially in cases with intraabdominal infections and serosal defects (p < 0.05). Conclusions: Preservation of the greater omentum reduces the formation of intestinal adhesions, especially in cases with underlying infections and serosal defects in abdominal closures using mesh grafts. This could be beneficial in related clinical situations in lowering the rate of intestinal fistulas, erosions, and obstructions that can be attributed to the formation of adhesions.

Research paper thumbnail of Effect of absorbent pads containing oregano essential oil on the shelf life extension of overwrap packed chicken drumsticks stored at four degrees Celsius

Poultry Science, 2009

The addition of sachets or pads containing volatile antimicrobial agents into packages has been t... more The addition of sachets or pads containing volatile antimicrobial agents into packages has been the most successful commercial application of antimicrobials to packaging. In this study, the effect of oregano (Origanum onites) essential oil on the extension of shelf life of overwrap packed fresh chicken drumsticks was investigated. Meat exudate absorbent pads were sprayed with 5 mL of oregano essential oil at a concentration of 1.5% in distillate water. Sampling was carried out at 0, 3, 5, and 7 d of the refrigerated storage. Total viable count, psychrotrophs, pseudomonads, members of the family Enterobacteriaceae, yeasts, and lactic acid bacteria were enumerated. Physicochemical analysis and sensorial evaluation were also conducted. The shelf life of fresh chicken drumsticks was approximately 3 d. Oregano essential oil extended product shelf life by approximately 2 d. Thus, incorporation of essential oils to absorbent pads may have supplementary applications in food packaging.

Research paper thumbnail of Different types of neural cell death in the cerebellum of the ataxia and male sterility (AMS) mutant mouse

Pathology International, 2006

To investigate the mechanisms(s) of age-dependent atrophy of the cerebellum of the ataxia and mal... more To investigate the mechanisms(s) of age-dependent atrophy of the cerebellum of the ataxia and male sterility (AMS) mouse at young age, the morphological changes were evaluated and the nature of neural cell death was examined. Dying Purkinje cells lacked characters of classical apoptosis except for light microscopic morphology, but their death was considered to be autonomous death triggered by the direct effect of ams mutation, because of the acute and near-complete disappearance and particular change of the cytoplasm. In contrast, in the granular layer, typical apoptotic bodies were recognized by electron microscopy, and substantial numbers of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end-labeling (TUNEL)-positive cells and activated caspase-3-positive cells were observed. Granule cell death was considered to be target-related apoptosis induced after post-synaptic Purkinje cell death, because the age-dependent changes in TUNEL-positive cell counts followed that of Purkinje cell loss and the peak value was still noted 1 week after total loss of Purkinje cells. These results indicate that both total and partial losses of Purkinje cells and granule cells, respectively, contributed to the atrophy of the AMS cerebellum. Furthermore, different types of neuronal death were recognized; the granule cell death was apoptotic while Purkinje cell death was different from that of classical apoptosis.