Charles Ndandala - Academia.edu (original) (raw)
Papers by Charles Ndandala
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Frontiers in Endocrinology
Insulin-like growth factor 3 (IGF3) induces ovarian maturation in teleosts; however, research on ... more Insulin-like growth factor 3 (IGF3) induces ovarian maturation in teleosts; however, research on its molecular regulatory mechanism remains deficient. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in various biological processes, including reproduction. In this study, circRNAs and miRNAs involved in IGF3-induced ovarian maturation were evaluated in spotted scat (Scatophagus argus). In ovarian tissues, we identified 176 differentially expressed (DE) circRNAs and 52 DE miRNAs between IGF3 treatment and control groups. Gene Ontology (GO) enrichment analyses showed that host genes of DE circRNAs and target genes of DE miRNAs were enriched for various processes with a high degree of overlap, including cellular process, reproduction, reproductive process, biological adhesion, growth, extracellular region, cell junction, catalytic activity, and transcription factor activity. Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included cell adhesion molecules, EC...
Animal biotechnology, Jan 29, 2017
Myostatin (MSTN), also named growth differentiation factor 8 (GDF8), is a transforming growth fac... more Myostatin (MSTN), also named growth differentiation factor 8 (GDF8), is a transforming growth factor-β (TGF-β) family member with a key role in the negative regulation of skeletal muscle growth. However, its role in ovarian folliculogenesis remains unclear. To provide us with a basis for understanding this role, we cloned MSTN and examined its expression patterns in water buffalo (Bubalus bubalis). The complete ORF of the water buffalo MSTN gene is 1,128 nucleotides, which encode a 375 amino acid protein and sharing 99% identity at the deducted amino acid level with that of Bos taurus. Protein sequence analysis showed that MSTN is a weakly acerbic extracellular protein, consisting of signal peptides at 18-19 sites, a TGF-β propeptide, and a TGF-β domain. RT-PCR analyses demonstrated that water buffalo MSTN was expressed in multiple tissues but not limited to muscle. Immunohistochemistry staining confirmed the presence of MSTN in oocytes and granulosal cells. To our knowledge, this i...
Frontiers in Marine Science
Fish venom has several biological activities, including enzyme activity, cytotoxicity, neurotoxic... more Fish venom has several biological activities, including enzyme activity, cytotoxicity, neurotoxicity, muscular toxicity, haemolytic, and cardiotoxicity, when they enter other species or a human being, they disrupt the physiological systems. Transcriptomic analysis of the fish venom glands revealed a large number of proteins relevant to the pharmacological activity even though they are not well-studied. The limitations in studying fish venoms also have an impact on their molecular characterization. This is partly because of the nature of fish venoms, as they are extremely unstable at normal ambient temperatures making them difficult to study. Venomous fish inhabit both marine and freshwater environments, they have specialized venom-delivery apparatuses. Venom delivery systems have evolved in a various animal species, originally for different purposes including defense, competition, as well as predation. In coastal areas, fish stings are a major problem because they have a serious tox...
Aquaculture reports, 2022
The Growth hormone (GH)-insulin-like growth factors (IGFs) axis plays a central role as the endoc... more The Growth hormone (GH)-insulin-like growth factors (IGFs) axis plays a central role as the endocrine regulator of physiological functions in teleost fish and other vertebrates. The basic mechanism of the GH-IGF system is
well-known to be useful in a different manner, but it seems to operate differently between species. We have pointed out different aspects related to IGF family genes and their GH dependence that seems to vary according to species. We discussed the effects of nutritional status, and physiological functions of IGFs in teleost gonads to facilitate somatic growth as well as embryonic development and oocyte maturation regulation. We found that nutrients and diet composition are environmental factors that influence somatic growth. The effects of fasting and re-feeding serving as key factors in the expression of IGF genes in different tissues were briefly described, whereby we have found that prolonged fasting causes ceasing of growth but significant elevation of GH concentration. Also, the IGFs have been reported to play significant roles in regulating both male and female gonad development. In addition, IGF3 is constantly expressed throughout all stages of embryogenesis. Therefore, improving our knowledge and understanding of these interactions will impact the design of better feeding intake and reproduction approaches to maximize growth performance, fish well-being, and maximum production of teleosts. This study is intended as a summary to provide the reader with a modern, and unique perspective on the past, present, and future of IGF research in teleosts. Therefore, we expect that gaps of knowledge will be exposed and stimulate future research areas related to IGFs in teleost fish with respect to growth and reproduction as well as other physiological functions.
Frontiers in Marine Science
In the aquaculture industry, fish oil is widely used as a nutritional supplement to promote the g... more In the aquaculture industry, fish oil is widely used as a nutritional supplement to promote the gonadal maturation of broodstocks, while the mechanism of fish oil on ovary development remain unclear. Herein, female adult spotted scat (Scatophagus argus) with most ovaries at phase II were fed with diets containing 8% soybean oil (SO) or 8% fish oil (FO) for 60 days. The final average fish body weight was similar between FO and SO groups. The average gonadosomatic index (GSI) of FO group was higher (non-significant) than that of SO group. Finally, the phase IV ovary of the FO and SO groups were 7 and 5 out of 10, respectively. The serum estradiol (E2) level of the FO group was significantly higher than that of the SO group. The proportions of n-3 long-chain polyunsaturated fatty acids (LC-PUFA, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)) in the ovaries were much higher in the FO group than in the SO group. These results suggest that the maturity level of the FO...
Frontiers in Endocrinology, May 2, 2022
processing, genetic information processing, diseases, metabolism and organismal systems. The resu... more processing, genetic information processing, diseases, metabolism and organismal systems. The results from this study provide a the oretical basis for studying the effects of sbGnRH on the physiology, biochemistry and metabolic enzyme activities of liver in pompano.
Science of The Total Environment
Fishes
Neuropeptide Y (Npy) is an intricate neuropeptide regulating numerous physiological processes. It... more Neuropeptide Y (Npy) is an intricate neuropeptide regulating numerous physiological processes. It is a highly conserved peptide known to improve feed intake in many vertebrates, including fishes. To enlighten the mechanism of Npy in spotted scat feed intake control, we cloned and identified the Npy cDNA sequence. We further examined its expression in some tissues and explored its expression effects at different time frames (hours and days). Here, we discovered that spotted scat Npy comprised a 300 bp open reading frame (ORF) and a 99 amino acid sequence. Npy was identified to be expressed in all tissues examined. Using in situ hybridization examination, we proved that npy has a wide expression in the brain of the spotted scat. Furthermore, the expression of npy in the hypothalamus significantly increased one hour after feeding (p < 0.05). Further, it was revealed that npy expression significantly increased in fish that were fasted for up to 5 days and significantly increased afte...
Aquaculture Reports, 2022
The Growth hormone (GH)-insulin-like growth factors (IGFs) axis plays a central role as the endoc... more The Growth hormone (GH)-insulin-like growth factors (IGFs) axis plays a central role as the endocrine regulator of physiological functions in teleost fish and other vertebrates. The basic mechanism of the GH-IGF system is well-known to be useful in a different manner, but it seems to operate differently between species. We have
pointed out different aspects related to IGF family genes and their GH dependence that seems to vary according to species. We discussed the effects of nutritional status, and physiological functions of IGFs in teleost gonads to
facilitate somatic growth as well as embryonic development and oocyte maturation regulation. We found that nutrients and diet composition are environmental factors that influence somatic growth. The effects of fasting and re-feeding serving as key factors in the expression of IGF genes in different tissues were briefly described, whereby we have found that prolonged fasting causes ceasing of growth but significant elevation of GH concentration. Also, the IGFs have been reported to play significant roles in regulating both male and female gonad development. In addition, IGF3 is constantly expressed throughout all stages of embryogenesis. Therefore,
improving our knowledge and understanding of these interactions will impact the design of better feeding intake and reproduction approaches to maximize growth performance, fish well-being, and maximum production of teleosts. This study is intended as a summary to provide the reader with a modern, and unique perspective on the past, present, and future of IGF research in teleosts. Therefore, we expect that gaps of knowledge will be exposed and stimulate future research areas related to IGFs in teleost fish with respect to growth and reproduction as well as other physiological functions.
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Frontiers in Endocrinology
Insulin-like growth factor 3 (IGF3) induces ovarian maturation in teleosts; however, research on ... more Insulin-like growth factor 3 (IGF3) induces ovarian maturation in teleosts; however, research on its molecular regulatory mechanism remains deficient. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in various biological processes, including reproduction. In this study, circRNAs and miRNAs involved in IGF3-induced ovarian maturation were evaluated in spotted scat (Scatophagus argus). In ovarian tissues, we identified 176 differentially expressed (DE) circRNAs and 52 DE miRNAs between IGF3 treatment and control groups. Gene Ontology (GO) enrichment analyses showed that host genes of DE circRNAs and target genes of DE miRNAs were enriched for various processes with a high degree of overlap, including cellular process, reproduction, reproductive process, biological adhesion, growth, extracellular region, cell junction, catalytic activity, and transcription factor activity. Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included cell adhesion molecules, EC...
Animal biotechnology, Jan 29, 2017
Myostatin (MSTN), also named growth differentiation factor 8 (GDF8), is a transforming growth fac... more Myostatin (MSTN), also named growth differentiation factor 8 (GDF8), is a transforming growth factor-β (TGF-β) family member with a key role in the negative regulation of skeletal muscle growth. However, its role in ovarian folliculogenesis remains unclear. To provide us with a basis for understanding this role, we cloned MSTN and examined its expression patterns in water buffalo (Bubalus bubalis). The complete ORF of the water buffalo MSTN gene is 1,128 nucleotides, which encode a 375 amino acid protein and sharing 99% identity at the deducted amino acid level with that of Bos taurus. Protein sequence analysis showed that MSTN is a weakly acerbic extracellular protein, consisting of signal peptides at 18-19 sites, a TGF-β propeptide, and a TGF-β domain. RT-PCR analyses demonstrated that water buffalo MSTN was expressed in multiple tissues but not limited to muscle. Immunohistochemistry staining confirmed the presence of MSTN in oocytes and granulosal cells. To our knowledge, this i...
Frontiers in Marine Science
Fish venom has several biological activities, including enzyme activity, cytotoxicity, neurotoxic... more Fish venom has several biological activities, including enzyme activity, cytotoxicity, neurotoxicity, muscular toxicity, haemolytic, and cardiotoxicity, when they enter other species or a human being, they disrupt the physiological systems. Transcriptomic analysis of the fish venom glands revealed a large number of proteins relevant to the pharmacological activity even though they are not well-studied. The limitations in studying fish venoms also have an impact on their molecular characterization. This is partly because of the nature of fish venoms, as they are extremely unstable at normal ambient temperatures making them difficult to study. Venomous fish inhabit both marine and freshwater environments, they have specialized venom-delivery apparatuses. Venom delivery systems have evolved in a various animal species, originally for different purposes including defense, competition, as well as predation. In coastal areas, fish stings are a major problem because they have a serious tox...
Aquaculture reports, 2022
The Growth hormone (GH)-insulin-like growth factors (IGFs) axis plays a central role as the endoc... more The Growth hormone (GH)-insulin-like growth factors (IGFs) axis plays a central role as the endocrine regulator of physiological functions in teleost fish and other vertebrates. The basic mechanism of the GH-IGF system is
well-known to be useful in a different manner, but it seems to operate differently between species. We have pointed out different aspects related to IGF family genes and their GH dependence that seems to vary according to species. We discussed the effects of nutritional status, and physiological functions of IGFs in teleost gonads to facilitate somatic growth as well as embryonic development and oocyte maturation regulation. We found that nutrients and diet composition are environmental factors that influence somatic growth. The effects of fasting and re-feeding serving as key factors in the expression of IGF genes in different tissues were briefly described, whereby we have found that prolonged fasting causes ceasing of growth but significant elevation of GH concentration. Also, the IGFs have been reported to play significant roles in regulating both male and female gonad development. In addition, IGF3 is constantly expressed throughout all stages of embryogenesis. Therefore, improving our knowledge and understanding of these interactions will impact the design of better feeding intake and reproduction approaches to maximize growth performance, fish well-being, and maximum production of teleosts. This study is intended as a summary to provide the reader with a modern, and unique perspective on the past, present, and future of IGF research in teleosts. Therefore, we expect that gaps of knowledge will be exposed and stimulate future research areas related to IGFs in teleost fish with respect to growth and reproduction as well as other physiological functions.
Frontiers in Marine Science
In the aquaculture industry, fish oil is widely used as a nutritional supplement to promote the g... more In the aquaculture industry, fish oil is widely used as a nutritional supplement to promote the gonadal maturation of broodstocks, while the mechanism of fish oil on ovary development remain unclear. Herein, female adult spotted scat (Scatophagus argus) with most ovaries at phase II were fed with diets containing 8% soybean oil (SO) or 8% fish oil (FO) for 60 days. The final average fish body weight was similar between FO and SO groups. The average gonadosomatic index (GSI) of FO group was higher (non-significant) than that of SO group. Finally, the phase IV ovary of the FO and SO groups were 7 and 5 out of 10, respectively. The serum estradiol (E2) level of the FO group was significantly higher than that of the SO group. The proportions of n-3 long-chain polyunsaturated fatty acids (LC-PUFA, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)) in the ovaries were much higher in the FO group than in the SO group. These results suggest that the maturity level of the FO...
Frontiers in Endocrinology, May 2, 2022
processing, genetic information processing, diseases, metabolism and organismal systems. The resu... more processing, genetic information processing, diseases, metabolism and organismal systems. The results from this study provide a the oretical basis for studying the effects of sbGnRH on the physiology, biochemistry and metabolic enzyme activities of liver in pompano.
Science of The Total Environment
Fishes
Neuropeptide Y (Npy) is an intricate neuropeptide regulating numerous physiological processes. It... more Neuropeptide Y (Npy) is an intricate neuropeptide regulating numerous physiological processes. It is a highly conserved peptide known to improve feed intake in many vertebrates, including fishes. To enlighten the mechanism of Npy in spotted scat feed intake control, we cloned and identified the Npy cDNA sequence. We further examined its expression in some tissues and explored its expression effects at different time frames (hours and days). Here, we discovered that spotted scat Npy comprised a 300 bp open reading frame (ORF) and a 99 amino acid sequence. Npy was identified to be expressed in all tissues examined. Using in situ hybridization examination, we proved that npy has a wide expression in the brain of the spotted scat. Furthermore, the expression of npy in the hypothalamus significantly increased one hour after feeding (p < 0.05). Further, it was revealed that npy expression significantly increased in fish that were fasted for up to 5 days and significantly increased afte...
Aquaculture Reports, 2022
The Growth hormone (GH)-insulin-like growth factors (IGFs) axis plays a central role as the endoc... more The Growth hormone (GH)-insulin-like growth factors (IGFs) axis plays a central role as the endocrine regulator of physiological functions in teleost fish and other vertebrates. The basic mechanism of the GH-IGF system is well-known to be useful in a different manner, but it seems to operate differently between species. We have
pointed out different aspects related to IGF family genes and their GH dependence that seems to vary according to species. We discussed the effects of nutritional status, and physiological functions of IGFs in teleost gonads to
facilitate somatic growth as well as embryonic development and oocyte maturation regulation. We found that nutrients and diet composition are environmental factors that influence somatic growth. The effects of fasting and re-feeding serving as key factors in the expression of IGF genes in different tissues were briefly described, whereby we have found that prolonged fasting causes ceasing of growth but significant elevation of GH concentration. Also, the IGFs have been reported to play significant roles in regulating both male and female gonad development. In addition, IGF3 is constantly expressed throughout all stages of embryogenesis. Therefore,
improving our knowledge and understanding of these interactions will impact the design of better feeding intake and reproduction approaches to maximize growth performance, fish well-being, and maximum production of teleosts. This study is intended as a summary to provide the reader with a modern, and unique perspective on the past, present, and future of IGF research in teleosts. Therefore, we expect that gaps of knowledge will be exposed and stimulate future research areas related to IGFs in teleost fish with respect to growth and reproduction as well as other physiological functions.