Chetan Bhalgat - Academia.edu (original) (raw)

Papers by Chetan Bhalgat

Eurasian Journal of Analytical Chemistry, Dec 7, 2016

A simple and sensitive headspace gas chromatographic method has been developed and validated for ... more A simple and sensitive headspace gas chromatographic method has been developed and validated for simultaneous determination of isopropyl alcohol and methylene chloride in zopiclone tablets. The separation was achieved on 75 m long DB-624 fused silica column, 0.53 mm inner diameter and 3 μ film thickness using nitrogen as a carrier gas at 6 ml/min flow and FID as a detector. The developed gas chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for all the solvents. The limit of detection of isopropyl alcohol and methylene chloride was found to be 250 μg/tablet and 90 μg/tablet, respectively.

Research Journal of Pharmacy and Technology, 2011

The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole have been synthesized by the reaction ... more The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole have been synthesized by the reaction of Benzofuran chalcone with hydroxylamine hydrochloride in presence of sodium acetate in ethanol. All the compounds were synthesized by conventional method and characterized by IR, 1H NMR and mass spectral data. The new synthesized derivatives I1-I7 was evaluated for in vitro cytotoxic activity on HeLa cell lines. Compounds I1-I7 was scheduled for evaluation against full panel of human cervical cancer cell lines at the minimum seven concentrations at two fold dilutions.

This review discusses forced degradation methodology and validation as per regulatory aspects of ... more This review discusses forced degradation methodology and validation as per regulatory aspects of investigations of degradation of active pharmaceutical ingredients. It also highlights the prediction of degradants of drug substances and degradation pathways and development of stability indicating analytical methods. Furthermore, review discusses analytical methodology of various conventional and hyphenated techniques for degradant separation and characterizations are summarized in detail. Forced degradation is a degradation of new drug substance and drug product at conditions more severe than accelerated conditions. It is required to demonstrate specificity of stability indicating methods and also provides an insight into degradation pathways of degradation products of the drug substance and helps in elucidation of the structure of the degradation products. Forced degradation studies show the chemical behavior of the molecule which in turn helps in the development of formulation and ...

Research Journal of Pharmacy and Technology, 2011

Amino group of thiazole moiety have been treated with various aromatic aldehydes gave correspondi... more Amino group of thiazole moiety have been treated with various aromatic aldehydes gave corresponding Schiff's bases, 3A-G. These Schiff's bases have been reacted with thioglycolic acid, anhydrous zinc chloride and N,N�-dimethyl Formamide as a solvent to get corresponding novel thiazolidinone derivatives, 4A-G. Structures of all the newly synthesized compounds were confirmed by IR, 1 H NMR, and mass spectral data. The synthesized compounds were evaluated for their antioxidant activity. Few compounds were found with potent antioxidant activity.

A few methods are available for routine analysis of sitagliptinphosphate and metformin hydrochlor... more A few methods are available for routine analysis of sitagliptinphosphate and metformin hydrochloride. Here we have developed a simple, precise and stability-indicating HPLC method and validated for the simultaneous determination of sitagliptinphosphate and metformin hydrochloride in pharmaceutical dosage form.The method involves the use of easily available inexpensive laboratory reagents. The method was carried out on a Supelco Column (25cm×4.6mm i.d; particle size 5-microns) with a mobile phase consisting of (70:30 v/v Acetonitrile: ammonium acetate buffer) at a flow rate of 0.8 ml/min.The retention time of the sitagliptin and metformin was about 6.54 and 4.24mins respectively. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The described method was linear over a concentration range of 10-50 μg/ml and 1-5 μg/ml for the assay of metformin and sitagliptin respectivelywith a correlation coefficient of 0.999.The accuracy (rec...

In the present study, we have reported in vitro antibacterial activity of our earlier reported co... more In the present study, we have reported in vitro antibacterial activity of our earlier reported compounds by serial dilution method. Among the compounds tested, few were found to be most effective antibacterial agents. Compounds containing thiazolo and quinolone ring may imparts better activity. The hydrazine substitution on pyrimidine may be responsible for reducing the activity. The results revealed that the synthesized compounds may be helpful to find out potential lead for future drug discovery.

In the present study, we have synthesized novel pyrimidines (A-H). IR, 1H NMR and mass spectral d... more In the present study, we have synthesized novel pyrimidines (A-H). IR, 1H NMR and mass spectral data elucidated structure of newly synthesized compounds. Some of these novel derivatives showed moderate to potent in vitro antioxidant and anti-inflammatory activity.

In the present study, we have synthesized novel 1,3,4-thiadiazole derivatives. Elemental analysis... more In the present study, we have synthesized novel 1,3,4-thiadiazole derivatives. Elemental analysis, IR, 1H NMR and mass spectral data elucidated structure of newly synthesized compounds. Some of these novel derivatives showed moderate to potent in vitro antioxidant activity.

In the present study, we have synthesized 1,3,4-thiadiazole derivatives and were investigated for... more In the present study, we have synthesized 1,3,4-thiadiazole derivatives and were investigated for their in vitro antioxidant activity. The results revealed that, some of tested compounds showed potent antioxidant activity. Amongst all the compound 1d has shown good activity. While, compound 1e has shown moderate activity. The remaining tested compounds were found weakly active.

In the present study, we have carried out the synthesis of pyrimidine derivatives and were invest... more In the present study, we have carried out the synthesis of pyrimidine derivatives and were investigated for their in vitro antioxidant activity. The results revealed that, some of tested compounds showed potent antioxidant activity. Amongst all the compound 1d has shown good activity. While, compound 1e has shown moderate activity. The remaining tested compounds were found weakly active.

Journal of Pharmaceutics & Drug Delivery Research

Application of an LC–MS/MS Method in Quantification of Hexyl Chloroformate Content in Dabigatran ... more Application of an LC–MS/MS Method in Quantification of Hexyl Chloroformate Content in Dabigatran Etexilate Mesylate Abstract A selective and sensitive liquid chromatography–mass spectrometry (AB SCIEX QTRAP 5500) method was developed for quantitative determination of genotoxic impurity, hexyl chloroformate, in drug substance. The method was developed by derivatizing the hexyl chloroformate (HCF) to complex compound hexyl benzylcarbamate (HBC) by reaction with benzylamine. This method provided good sensitivity for quantification of hexyl chloroformate at a concentration of 10 ppm in 10 mg/mL DEM sample solution. The compounds were chromatographed under isocratic condition on poroshell EC-C18 (2.7 μm particle packed in 4.6 x 50 mm column) column with mobile phase of 0.1% v/v ammonium hydroxide in water (pH adjusted to 6 using acetic acid) and acetonitrile in ratio of 1:1 v/v at the flow rate of 1.0 ml/minute with triple quadrupole Q-Trap 5500 mass spectrometer operated in multiple reaction monitoring mode (MRM). To produce transition ion of 236/152 the molecular mass of 236(M+H) was used as molecular ion. Positive mode electro spray ionization (ESI) was employed as the ionization source. The developed method was validated in terms of specificity, limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy, and robustness. The LOD & LOQ were found at 2.1 and 4.2 ppm, respectively.

Asian Journal of Research in Chemistry, 2014

In the present study, a series of novel pyrimidines were synthesized. Structures of the synthesiz... more In the present study, a series of novel pyrimidines were synthesized. Structures of the synthesized compounds were established by IR, 1H NMR and mass spectral data. All the newly synthesized compounds were screened for their in vitro antifungal activities. Among the compounds tested, few were found to be most effective antifungal agents. The results revealed that the synthesized compounds may be potential lead for future drug discovery.

The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole, I1-I7 was evaluated for in vitro anti... more The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole, I1-I7 was evaluated for in vitro anti-inflammatory activity. Amongst all the compound I4 has shown good activity. While, compound I5 has shown moderate activity. The remaining tested compounds were found weakly active.

No method is available for routine analysis of sitagliptin phosphate and metformin hydrochloride.... more No method is available for routine analysis of sitagliptin phosphate and metformin hydrochloride. Here we have developed simple, accurate and rapid UV spectrophotometric method for simultaneous estimation of sitagliptin phosphate and metformin hydrochloride in tablet formulation. The drug obeyed the Beer-Lambert's law and showed good correlation. The method based on solving of simultaneous equation using 273 nm (λmax of sitagliptin) and 237 nm (λmax of metformin) as two analytical wavelengths for both drugs in mixture of water and methanol (60:40) solvent. The method was found to be simple, rapid, accurate, precise and reproducible.

The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole have been synthesized by the reaction ... more The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole have been synthesized by the reaction of Benzofuran chalcone with hydroxylamine hydrochloride in presence of sodium acetate in ethanol. All the compounds were synthesized by conventional method and characterized by IR, 1 H NMR and mass spectral data. The new synthesized derivatives I1-I7 was evaluated for in vitro cytotoxic activity on HeLa cell lines. Compounds I1-I7 was scheduled for evaluation against full panel of human cervical cancer cell lines at the minimum seven concentrations at two fold dilutions. INTRODUCTION:

In the present study, we have synthesized novel 1,3,4-thiadiazole derivatives. Elemental analysis... more In the present study, we have synthesized novel 1,3,4-thiadiazole derivatives. Elemental analysis, IR, 1 H NMR and mass spectral data elucidated structure of newly synthesized compounds. Some of these novel derivatives showed moderate to potent in vitro antioxidant activity. INTRODUCTION:

Journal of scientific and industrial research

A new starch powder isolated by steeping process from pepino fruits (Solanum muricatum Aiton) as ... more A new starch powder isolated by steeping process from pepino fruits (Solanum muricatum Aiton) as tablet disintegrant was compared with maize starch BP (MS) in paracetamol tablets prepared via wet granulation method. Increasing concentration resulted in slight increase in hardness and decrease in friability for MS, whereas hardness slightly decreased and friability marginally increased for PS. PS absorbed least moisture followed by MS. PS formulations showed longer disintegration time than MS. However disintegration time was comparable at 10% w/w concentration: PS, 4.7; and MS, 5.0 min. PS is likely to initiate disintegration by swelling and capillary action. Drug dissolution (70%) was within 30 min. Thus PS powder appears to be a suitable substitute for MS as internal disintegrant in paracetamol tablet formulations.

Asian Journal of Research in Chemistry, 2015

In the present study, a series of pyrimidines were synthesized by microwave technique. The microw... more In the present study, a series of pyrimidines were synthesized by microwave technique. The microwave technique synthesis was found advantageous over conventional by reducing reaction time, increasing yield and lesser usage of solvent. Structures of the synthesized compounds were established by IR, 1H NMR and mass spectral data. Selected synthesized compounds were screened for their in vitro antifungal activity by method to find out the Minimum Inhibitory Concentration. Among the compounds tested, few were found to be most effective antifungal agents. The results revealed that the synthesized compounds may be potential lead for future drug discovery.

... How to cite this URL: Halve AK, Dubey R, Bhadauria D, Bhaskar B, Bhadauria R. Synthesis, anti... more ... How to cite this URL: Halve AK, Dubey R, Bhadauria D, Bhaskar B, Bhadauria R. Synthesis, antimicrobial screening and structure-activity relationship of some novel 2-hydroxy-5-(nitro-substituted phenylazo ... 1. Dutta, MM, Goswami, BN and Katkay, JCS, J. Indian Chem. ...

Eurasian Journal of Analytical Chemistry, Dec 7, 2016

A simple and sensitive headspace gas chromatographic method has been developed and validated for ... more A simple and sensitive headspace gas chromatographic method has been developed and validated for simultaneous determination of isopropyl alcohol and methylene chloride in zopiclone tablets. The separation was achieved on 75 m long DB-624 fused silica column, 0.53 mm inner diameter and 3 μ film thickness using nitrogen as a carrier gas at 6 ml/min flow and FID as a detector. The developed gas chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for all the solvents. The limit of detection of isopropyl alcohol and methylene chloride was found to be 250 μg/tablet and 90 μg/tablet, respectively.

Research Journal of Pharmacy and Technology, 2011

The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole have been synthesized by the reaction ... more The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole have been synthesized by the reaction of Benzofuran chalcone with hydroxylamine hydrochloride in presence of sodium acetate in ethanol. All the compounds were synthesized by conventional method and characterized by IR, 1H NMR and mass spectral data. The new synthesized derivatives I1-I7 was evaluated for in vitro cytotoxic activity on HeLa cell lines. Compounds I1-I7 was scheduled for evaluation against full panel of human cervical cancer cell lines at the minimum seven concentrations at two fold dilutions.

This review discusses forced degradation methodology and validation as per regulatory aspects of ... more This review discusses forced degradation methodology and validation as per regulatory aspects of investigations of degradation of active pharmaceutical ingredients. It also highlights the prediction of degradants of drug substances and degradation pathways and development of stability indicating analytical methods. Furthermore, review discusses analytical methodology of various conventional and hyphenated techniques for degradant separation and characterizations are summarized in detail. Forced degradation is a degradation of new drug substance and drug product at conditions more severe than accelerated conditions. It is required to demonstrate specificity of stability indicating methods and also provides an insight into degradation pathways of degradation products of the drug substance and helps in elucidation of the structure of the degradation products. Forced degradation studies show the chemical behavior of the molecule which in turn helps in the development of formulation and ...

Research Journal of Pharmacy and Technology, 2011

Amino group of thiazole moiety have been treated with various aromatic aldehydes gave correspondi... more Amino group of thiazole moiety have been treated with various aromatic aldehydes gave corresponding Schiff's bases, 3A-G. These Schiff's bases have been reacted with thioglycolic acid, anhydrous zinc chloride and N,N�-dimethyl Formamide as a solvent to get corresponding novel thiazolidinone derivatives, 4A-G. Structures of all the newly synthesized compounds were confirmed by IR, 1 H NMR, and mass spectral data. The synthesized compounds were evaluated for their antioxidant activity. Few compounds were found with potent antioxidant activity.

A few methods are available for routine analysis of sitagliptinphosphate and metformin hydrochlor... more A few methods are available for routine analysis of sitagliptinphosphate and metformin hydrochloride. Here we have developed a simple, precise and stability-indicating HPLC method and validated for the simultaneous determination of sitagliptinphosphate and metformin hydrochloride in pharmaceutical dosage form.The method involves the use of easily available inexpensive laboratory reagents. The method was carried out on a Supelco Column (25cm×4.6mm i.d; particle size 5-microns) with a mobile phase consisting of (70:30 v/v Acetonitrile: ammonium acetate buffer) at a flow rate of 0.8 ml/min.The retention time of the sitagliptin and metformin was about 6.54 and 4.24mins respectively. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The described method was linear over a concentration range of 10-50 μg/ml and 1-5 μg/ml for the assay of metformin and sitagliptin respectivelywith a correlation coefficient of 0.999.The accuracy (rec...

In the present study, we have reported in vitro antibacterial activity of our earlier reported co... more In the present study, we have reported in vitro antibacterial activity of our earlier reported compounds by serial dilution method. Among the compounds tested, few were found to be most effective antibacterial agents. Compounds containing thiazolo and quinolone ring may imparts better activity. The hydrazine substitution on pyrimidine may be responsible for reducing the activity. The results revealed that the synthesized compounds may be helpful to find out potential lead for future drug discovery.

In the present study, we have synthesized novel pyrimidines (A-H). IR, 1H NMR and mass spectral d... more In the present study, we have synthesized novel pyrimidines (A-H). IR, 1H NMR and mass spectral data elucidated structure of newly synthesized compounds. Some of these novel derivatives showed moderate to potent in vitro antioxidant and anti-inflammatory activity.

In the present study, we have synthesized novel 1,3,4-thiadiazole derivatives. Elemental analysis... more In the present study, we have synthesized novel 1,3,4-thiadiazole derivatives. Elemental analysis, IR, 1H NMR and mass spectral data elucidated structure of newly synthesized compounds. Some of these novel derivatives showed moderate to potent in vitro antioxidant activity.

In the present study, we have synthesized 1,3,4-thiadiazole derivatives and were investigated for... more In the present study, we have synthesized 1,3,4-thiadiazole derivatives and were investigated for their in vitro antioxidant activity. The results revealed that, some of tested compounds showed potent antioxidant activity. Amongst all the compound 1d has shown good activity. While, compound 1e has shown moderate activity. The remaining tested compounds were found weakly active.

In the present study, we have carried out the synthesis of pyrimidine derivatives and were invest... more In the present study, we have carried out the synthesis of pyrimidine derivatives and were investigated for their in vitro antioxidant activity. The results revealed that, some of tested compounds showed potent antioxidant activity. Amongst all the compound 1d has shown good activity. While, compound 1e has shown moderate activity. The remaining tested compounds were found weakly active.

Journal of Pharmaceutics & Drug Delivery Research

Application of an LC–MS/MS Method in Quantification of Hexyl Chloroformate Content in Dabigatran ... more Application of an LC–MS/MS Method in Quantification of Hexyl Chloroformate Content in Dabigatran Etexilate Mesylate Abstract A selective and sensitive liquid chromatography–mass spectrometry (AB SCIEX QTRAP 5500) method was developed for quantitative determination of genotoxic impurity, hexyl chloroformate, in drug substance. The method was developed by derivatizing the hexyl chloroformate (HCF) to complex compound hexyl benzylcarbamate (HBC) by reaction with benzylamine. This method provided good sensitivity for quantification of hexyl chloroformate at a concentration of 10 ppm in 10 mg/mL DEM sample solution. The compounds were chromatographed under isocratic condition on poroshell EC-C18 (2.7 μm particle packed in 4.6 x 50 mm column) column with mobile phase of 0.1% v/v ammonium hydroxide in water (pH adjusted to 6 using acetic acid) and acetonitrile in ratio of 1:1 v/v at the flow rate of 1.0 ml/minute with triple quadrupole Q-Trap 5500 mass spectrometer operated in multiple reaction monitoring mode (MRM). To produce transition ion of 236/152 the molecular mass of 236(M+H) was used as molecular ion. Positive mode electro spray ionization (ESI) was employed as the ionization source. The developed method was validated in terms of specificity, limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy, and robustness. The LOD & LOQ were found at 2.1 and 4.2 ppm, respectively.

Asian Journal of Research in Chemistry, 2014

In the present study, a series of novel pyrimidines were synthesized. Structures of the synthesiz... more In the present study, a series of novel pyrimidines were synthesized. Structures of the synthesized compounds were established by IR, 1H NMR and mass spectral data. All the newly synthesized compounds were screened for their in vitro antifungal activities. Among the compounds tested, few were found to be most effective antifungal agents. The results revealed that the synthesized compounds may be potential lead for future drug discovery.

The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole, I1-I7 was evaluated for in vitro anti... more The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole, I1-I7 was evaluated for in vitro anti-inflammatory activity. Amongst all the compound I4 has shown good activity. While, compound I5 has shown moderate activity. The remaining tested compounds were found weakly active.

No method is available for routine analysis of sitagliptin phosphate and metformin hydrochloride.... more No method is available for routine analysis of sitagliptin phosphate and metformin hydrochloride. Here we have developed simple, accurate and rapid UV spectrophotometric method for simultaneous estimation of sitagliptin phosphate and metformin hydrochloride in tablet formulation. The drug obeyed the Beer-Lambert's law and showed good correlation. The method based on solving of simultaneous equation using 273 nm (λmax of sitagliptin) and 237 nm (λmax of metformin) as two analytical wavelengths for both drugs in mixture of water and methanol (60:40) solvent. The method was found to be simple, rapid, accurate, precise and reproducible.

The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole have been synthesized by the reaction ... more The 3-(1-benzofuran-2-yl)-5-(substituted phenyl) isoxazole have been synthesized by the reaction of Benzofuran chalcone with hydroxylamine hydrochloride in presence of sodium acetate in ethanol. All the compounds were synthesized by conventional method and characterized by IR, 1 H NMR and mass spectral data. The new synthesized derivatives I1-I7 was evaluated for in vitro cytotoxic activity on HeLa cell lines. Compounds I1-I7 was scheduled for evaluation against full panel of human cervical cancer cell lines at the minimum seven concentrations at two fold dilutions. INTRODUCTION:

In the present study, we have synthesized novel 1,3,4-thiadiazole derivatives. Elemental analysis... more In the present study, we have synthesized novel 1,3,4-thiadiazole derivatives. Elemental analysis, IR, 1 H NMR and mass spectral data elucidated structure of newly synthesized compounds. Some of these novel derivatives showed moderate to potent in vitro antioxidant activity. INTRODUCTION:

Journal of scientific and industrial research

A new starch powder isolated by steeping process from pepino fruits (Solanum muricatum Aiton) as ... more A new starch powder isolated by steeping process from pepino fruits (Solanum muricatum Aiton) as tablet disintegrant was compared with maize starch BP (MS) in paracetamol tablets prepared via wet granulation method. Increasing concentration resulted in slight increase in hardness and decrease in friability for MS, whereas hardness slightly decreased and friability marginally increased for PS. PS absorbed least moisture followed by MS. PS formulations showed longer disintegration time than MS. However disintegration time was comparable at 10% w/w concentration: PS, 4.7; and MS, 5.0 min. PS is likely to initiate disintegration by swelling and capillary action. Drug dissolution (70%) was within 30 min. Thus PS powder appears to be a suitable substitute for MS as internal disintegrant in paracetamol tablet formulations.

Asian Journal of Research in Chemistry, 2015

In the present study, a series of pyrimidines were synthesized by microwave technique. The microw... more In the present study, a series of pyrimidines were synthesized by microwave technique. The microwave technique synthesis was found advantageous over conventional by reducing reaction time, increasing yield and lesser usage of solvent. Structures of the synthesized compounds were established by IR, 1H NMR and mass spectral data. Selected synthesized compounds were screened for their in vitro antifungal activity by method to find out the Minimum Inhibitory Concentration. Among the compounds tested, few were found to be most effective antifungal agents. The results revealed that the synthesized compounds may be potential lead for future drug discovery.

... How to cite this URL: Halve AK, Dubey R, Bhadauria D, Bhaskar B, Bhadauria R. Synthesis, anti... more ... How to cite this URL: Halve AK, Dubey R, Bhadauria D, Bhaskar B, Bhadauria R. Synthesis, antimicrobial screening and structure-activity relationship of some novel 2-hydroxy-5-(nitro-substituted phenylazo ... 1. Dutta, MM, Goswami, BN and Katkay, JCS, J. Indian Chem. ...