Ching-Shuang Wu - Academia.edu (original) (raw)

Papers by Ching-Shuang Wu

PubMed, Jul 1, 2022

Background: The latest European System for Cardiac Operative Risk Evaluation (EuroSCORE) II is a ... more Background: The latest European System for Cardiac Operative Risk Evaluation (EuroSCORE) II is a well-accepted risk evaluation system for mortality in cardiac surgery in Europe. Objectives: To determine the performance of this new model in Taiwanese patients. Methods: Between January 2012 and December 2014, 657 patients underwent cardiac surgery at our institution. The EuroSCORE II scores of all patients were determined preoperatively. The short-term surgical outcomes of 30-day and in-hospital mortality were evaluated to assess the performance of the EuroSCORE II. Results: Of the 657 patients [192 women (29.22%); age 63.5 ± 12.68 years], the 30-day mortality rate was 5.48%, and the in-hospital mortality rate was 9.28%. The discrimination power of this new model was good in all populations, regardless of 30-day mortality or in-hospital mortality. Good accuracy was also noted in different procedures related to coronary artery bypass grafting, and good calibration was noted for cardiac procedures (p value > 0.05). When predicting surgical death within 30 days, the EuroSCORE II overestimated the risk (observed to expected: 0.79), but in-hospital mortality was underestimated (observed to expected: 1.33). The predictive ability [area under the curve (AUC) of the receiver operating characteristic (ROC) curve] and calibration of the EuroSCORE II for 30-day mortality (0.792) and in-hospital mortality (0.825) suggested that in-hospital mortality is a better endpoint for the EuroSCORE II. Conclusions: The new EuroSCORE II model performed well in predicting short-term outcomes among patients undergoing general cardiac surgeries. For short-term outcomes, in-hospital mortality was better than 30-day mortality as an indicator of surgical results, suggesting that it may be a better endpoint for the EuroSCORE II.

PubMed, May 1, 2002

To understand the relevance of serological parameters and eliciting allergens in the patients wit... more To understand the relevance of serological parameters and eliciting allergens in the patients with atopic dermatitis (AD) and patients with atopic dermatitis in combination with rhinitis (ADR), we compared the serum total IgE, major basic protein (MBP) and eosinophil cationic protein (ECP) levels and identified the sensitized allergens in these two groups. The serum levels of total IgE, MBP and ECP were not significantly different in these two groups. Patients with ADR had a higher positive rate of aeroallergens including housedust, mite (D.F), mite (D.P) and cockroach than patients with AD. However, a high positive rate of seafood allergens (include crab, shellfish and shrimp) was observed in patients with AD. The serum IgE level correlated well with serum MBP level (r = 0.603, P = 0. 019) in patients with ADR. There was a strong correlation between serum MBP and ECP level (r = 0.773, P = 0.005) in patients with AD and in patients with ADR (r = 0.721, P = 0.008). We also found that numbers of sensitized allergens correlated well with total IgE in patients with AD (r = 0.760, P = 0.001) and in patients with ADR (r = 0.487, P = 0.004). These results suggested that the aeroallergens are the most important allergens causing and aggravating atopic diseases in Taiwan. Seafood may play an important role in the pathogenesis of AD. We suggest the measurement of serum total IgE combined with Multiple Allergens Simultaneous Test-Chemiluminescent Assays(AST-CLA) test could be helpful in the diagnosis of atopic diseases.

Journal of Orthopaedic Surgery Taiwan, Mar 1, 1997

Sarcomas of soft tissue are uncommon. Many different methods of treatment have been developed, bu... more Sarcomas of soft tissue are uncommon. Many different methods of treatment have been developed, but surgical resection is still an effective option. This retrospective study of 26 patients with soft tissue sarcoma treated by surgery between 1987 and 1996 in Kaohsiung Medical College Hospital involved 12 male and 14 female patients, aged from 14to 77 with an average of 44 years. The follow-up period ranged from 6 months to 110 months. While we have found that histological grade and distant metastasis can be closely related to survival in soft tissue sarcoma, local recurrence, adjuvant radiotherapy or chemotherapy, tumor size and surgical procedure (amputation or limb-salvage) do not influence the survival rate. We have also confirmed that limb-salvage is an appropriated treatment for soft tissue sarcoma.

Biomarkers and Genomic Medicine, Sep 1, 2013

A weighted enzymatic chip array (WEnCA) can detect the gene expression of circulating tumor cells... more A weighted enzymatic chip array (WEnCA) can detect the gene expression of circulating tumor cells (CTCs) in the peripheral blood of patients with colorectal cancer (CRC). In this study, we used a reagent kit, the GeneCling CRC Enzymatic Gene Chip Detection Kit, which was specifically developed for the WEnCA operation platform to analyze the expression of 31 CRCrelated genes of CTCs in the peripheral blood of 30 CRC patients. We moreover evaluated the expression of the genes by simultaneously using real-time quantitative polymerase chain reaction (RT-QPCR). The results showed the overexpression rate of nine genesdDVL1, ELAVL4, CHRNB1, UBE2C, PSAT1, CEA, PTTG1, KRT19, and hTERTdwas greater than 90% in the CRC patients. This is concordant with the results of the original WEnCA operation procedure. Linear regression analysis demonstrated a high correlation (r Z 0.901; p < 0.0001) between the experimental data of the detection kit and RT-QPCR. The GeneCling CRC Enzymatic Gene Chip Detection Kit is easy, fast, and convenient to operate for detecting gene expression of CTCs from peripheral blood. However,

British Journal of Dermatology, May 1, 2005

tomography scan of the abdomen and bone marrow biopsy, which excluded systemic disease. As there ... more tomography scan of the abdomen and bone marrow biopsy, which excluded systemic disease. As there were no remnants of lesion, radiotherapy was not performed. Mycophenolate mofetil and tacrolimus ANH were reduced to half the previous dosage. There was no recurrence at 12-month follow-up. Cutaneous CD30+ ALTCL is characterized by the expression of CD30 (an activation marker for B or T cells) in more than 75% of cells, and can be subdivided mainly into a primary cutaneous form, defined by skin-only involvement at presentation, or a systemic form, with secondary skin involvement from a node-based lymphoma, or following transformation of mycosis fungoides. 5-6 The localization of PTLs in the skin, as well as the CD30+ ALTCL type, are very uncommon. 2-4 To our knowledge, only five cases of CD30+ PT ALTCL have been reported to date: three in kidney recipients, one in a heart transplant and the other in a combined liver and heart transplantation recipient. 2-4 Whereas classical primary cutaneous CD30+ ALTCL usually carries a good prognosis, CD30+ PT ALTCLs seem to pursue an aggressive course: the previously reported cases were characterized by multiple lesions at presentation and ⁄or a rapid appearance of new nodules within a few months. 2-4 In our patient, there was a unique tumour at presentation, and no further lesions appeared within 12 months, although we think that a longer follow-up is necessary to evaluate the clinical behaviour more thoroughly. We think that the small size of the lesion was the most favourable prognostic factor in our case, as it allowed for a complete excision of tumour. We also found strong positivity for MUM1 ⁄IRF4, which is thought to be crucial for lymphoid development and has been found to be strongly expressed in various types of leukaemia, Band T-cell lymphomas, including ALTCL. 7-9 MUM1 ⁄IRF4 expression is thought to be helpful for diagnostic purposes, whereas its prognostic role is controversial, probably depending on the type of leukaemia ⁄lymphoma considered. 7-9 MUM1 ⁄IRF4 was not investigated in the previously reported cases of CD30+ PT ALTCLs; it could be worthwhile to study its expression on a larger series of PTLs. The unusual clinical behaviour and histotype and the immunophenotype of our case may add knowledge to the spectrum of PTLs.

International Journal of Oncology, Feb 1, 2006

Contact Dermatitis, Dec 7, 2010

Background. Nurses are prone to develop hand dermatitis. Although an atopic constitution has been... more Background. Nurses are prone to develop hand dermatitis. Although an atopic constitution has been identified as a genetic risk factor, the behavioural risk factors associated with hand dermatitis in wet work conditions have not been fully explored. Objectives. This study aimed to clarify the impact of atopic eczema (fulfilling the diagnostic criteria during the past 1 year) on the occurrence of hand dermatitis and to identify the behavioural risk factors among non-atopic nurses with hand dermatitis. Methods. From August 2007 to July 2009, nurses from Kaohsiung Medical University Hospital were recruited. The associations between different risk factors and hand dermatitis were documented. In addition, the behavioural risk factors among non-atopic nurses were evaluated via observational study. Results. One thousand one hundred and thirty-two nurses participated in the first part of the study, which revealed that individuals with atopic eczema had a 3.76-fold increased risk for hand dermatitis. However, among 248 nurses with hand dermatitis, only 43 had atopic eczema. The observational study performed on 140 non-atopic nurses identified frequency of hand washing as the behavioural risk factor associated with hand dermatitis. Conclusions. Although atopic eczema is the major risk factor for hand dermatitis, those with atopic eczema constitute only 17% of nurses with hand dermatitis. Decreasing hand washing frequency is the most effective strategy to reduce the occurrence of hand dermatitis among non-atopic nurses.

Experimental Dermatology, Oct 20, 2011

The term &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp... more The term &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;hand dermatitis&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; describes inflammatory skin condition localized to the hands. Nurses working at hospital settings are prone to develop hand dermatitis. The current study aimed to evaluate whether certain genetic polymorphisms were associated with the development of atopic eczema or non-atopic hand dermatitis in Taiwanese population. Nurses of Kaohsiung Medical University Hospital were recruited. Atopic eczema, non-atopic hand dermatitis and normal control groups were identified. The serine protease inhibitor Kazal type 5 (SPINK5), filaggrin and interleukin-31 (IL-31) gene variants were compared between the diseased and control groups. Our results showed that rs2303070 T allele of SPINK5 (assuming recessive model; OR=3.58, 95% CI 1.63-7.84; P=0.0014) and rs7977932 G allele of IL-31 (assuming recessive model; OR=18.25, 95% CI =3.27-101.94; P=0.0009) were associated with increased risks of developing atopic eczema, while rs6892205 G allele of SPINK5 (assuming dominant model; OR=3.79, 95% CI 1.55-9.28; P=0.0036) was associated with the development of non-atopic hand dermatitis. In summary, our results showed that distinct SPINK5 and IL-31 gene variants were associated with the development of atopic eczema and non-atopic hand dermatitis. The barrier function, particularly those regulated by SPINK5, may play an important role in the development of both atopic eczema and non-atopic hand dermatitis.

Food & Function, 2016

Our objective was to investigate and compare the effects of heat-killed (HK) and live Lactobacill... more Our objective was to investigate and compare the effects of heat-killed (HK) and live Lactobacillus reuteri GMNL-263 (Lr263) on insulin resistance and its related complications in high-fat diet (HFD)-induced rats. Male Sprague-Dawley rats were fed with a HFD with either HK or live Lr263 for 12 weeks. The increases in the weight gain, serum glucose, insulin, and lipid profiles in the serum and liver observed in the HFD group were significantly reduced after HK or live Lr263 administration. Feeding HK or live Lr263 reversed the decreased number of probiotic bacteria and increased the number of pathogenic bacteria induced by high-fat treatment. The decreased intestinal barrier in the HFD group was markedly reversed by HK or live Lr263 treatments. The elevations of pro-inflammatory associated gene expressions in both adipose and hepatic tissues by high-fat administration were markedly decreased by HK or live Lr263 treatments. The increased macrophage infiltration noticed in adipose tissue after high-fat treatment was effectively suppressed by HK or live Lr263 consumption. The insulin resistance associated gene expressions in both adipose and hepatic tissues, which were downregulated in the HFD group, were markedly enhanced after HK or live Lr263 administration. HK or live Lr263 consumption significantly decreased hepatic lipogenic gene expressions stimulated by high-fat treatment. Administration of HK or live Lr263 significantly reduced hepatic oil red O staining and ameliorated the hepatic steatosis observed in high-fat treated rats. Our data suggested that similar to live Lr263, HK Lr263 exerted significant effects on attenuating obesityinduced metabolic abnormalities by reducing insulin resistance and hepatic steatosis formation.

Chinese Journal of Physiology, 2022

Journal of Dermatological Science, Dec 1, 2007

BioMed Research International, 2013

This study is to investigate multiple chemotherapeutic agent-and radiation-related genetic biomar... more This study is to investigate multiple chemotherapeutic agent-and radiation-related genetic biomarkers in locally advanced rectal cancer (LARC) patients following fluoropyrimidine-based concurrent chemoradiotherapy (CCRT) for response prediction. We initially selected 6 fluoropyrimidine metabolism-related genes (DPYD, ORPT, TYMS, TYMP, TK1, and TK2) and 3 radiotherapy response-related genes (GLUT1, HIF-1 , and HIF-2) as targets for gene expression identification in 60 LARC cancer specimens. Subsequently, a high-sensitivity weighted enzymatic chip array was designed and constructed to predict responses following CCRT. After CCRT, 39 of 60 (65%) LARC patients were classified as responders (pathological tumor regression grade 2 ∼ 4). Using a panel of multiple genetic biomarkers (chip), including DPYD, TYMS, TYMP, TK1, and TK2, at a cutoff value for 3 positive genes, a sensitivity of 89.7% and a specificity of 81% were obtained (AUC: 0.915; 95% CI: 0.840-0.991). Negative chip results were significantly correlated to poor CCRT responses (TRG 0-1) (= 0.014, hazard ratio: 22.704, 95% CI: 3.055-235.448 in multivariate analysis). Disease-free survival analysis showed significantly better survival rate in patients with positive chip results (= 0.0001). We suggest that a chip including DPYD, TYMS, TYMP, TK1, and TK2 genes is a potential tool to predict response in LARC following fluoropyrimidine-based CCRT.

Pigment Cell & Melanoma Research, Mar 15, 2013

Light exposure modulates development of living organisms. In the field of medicine, light has fre... more Light exposure modulates development of living organisms. In the field of medicine, light has frequently been used for regenerative purposes. Excimer light (308 nm) has demonstrated superior efficacy in treating vitiligo, a condition requiring development of melanoblasts and a model for studying nerve cell regeneration, as compared to narrow-band ultraviolet B (NBUVB; 311 nm). Using mouse-derived melanoblast cells to examine the pro-differentiation effects of these two light sources, we demonstrated that at equivalent fluence, excimer light induces melanoblast differentiation, while NBUVB failed to so. Mechanistically, activation of aryl hydrocarbon receptor pathway and nuclear translocation of epidermal growth factor receptor are involved in pro-differentiation effects of excimer light. Reduction in irradiance by filter abrogated the effects of excimer light in melanoblasts, even when equivalent fluence was delivered by the same light source. As ultraviolet B (UVB) irradiation is closely associated pigment cell development, future therapy employing UVB for pigmentation purposes should incorporate irradiance as a crucial specification.

Acta dermato-venereologica, 2013

British Journal of Dermatology, 2011

Topical tacrolimus (FK506) has been considered as a treatment option for treating vitiligo, a der... more Topical tacrolimus (FK506) has been considered as a treatment option for treating vitiligo, a dermatosis characterized by disappearance of melanocytes (MCs). Previous reports have shown that a significant portion of treated patients demonstrated follicular repigmentation, indicating that the activation of MC precursor cells residing in the outer root sheath of hair follicles played an important role during the tacrolimus-induced repigmentation process. To investigate the mechanisms involved in follicular pigmentation induced by topical tacrolimus. As stem cells of MC lineage are identified in the lower portion of mouse hair follicles throughout the hair cycle, immature mouse melanoblasts (MBs) derived from neural crest cells (NCCmelb4) were used for this study. Relevant maturation parameters were evaluated. Our results revealed that FK506 stimulated the expressions of protein kinase A, protein kinase C and phosphorylated p38 mitogen-activated protein kinase. However, cell motility, a parameter associated with MB differentiation, was not enhanced by FK506 treatment. Endothelin (ET)-3, a prodifferentiation factor of MBs, also failed to promote NCCmelb4 cell locomotion. Combining ET-3 and FK506, however, stimulated cell mobility. ET B receptor, which was not present in NCCmelb4 cells, was induced after FK506 treatment. In summary, we have shown that FK506 is an efficient differentiation-stimulating agent, especially for cells of neural origin. The clinical efficacy of topical tacrolimus on vitiligo may be enhanced by combination with ET-3.

DNA and Cell Biology, Sep 1, 2007

Urease is involved in Helicobacter pylori infection and survival in acid circumference. This stud... more Urease is involved in Helicobacter pylori infection and survival in acid circumference. This study explored the overexpression of H. pylori-associated urease mRNAs in human gastric cancers by using a well-established membrane array analysis method in our lab. Analysis of 30 gastric cancer tissue specimens and 30 paired adjacent normal tissues demonstrated that urease genes involved in H. pylori infection were upregulated in gastric cancer tissues. UreA, G, and I are predominant genotypes found in gastric cancer tissues. However, the mRNA levels of UreC and UreE were hardly to be found in both gastric cancer and normal tissues in our study. In addition, we treated NIH-3T3 cells with two kinds of H. pylori exudates [weak urease activity (HP-W) and strong urease activity (HP-S)], which contained 1.6, 3.1, 6.5, 13, and 25.9 pg/mL urease of HP-W exudates and 18, 36, 75, 150, and 300 pg/mL urease of HP-S exudates. NIH-3T3 cells were treated with these different concentration components for 24 h. Cell proliferation rate was elevated 2.7%, 9.9%, 18.9%, 36.6%, and 42.9%, respectively, after HP-W exudates were treated, and elevated 8.1%, 31.9%, 45.9%, 74.9%, and 81.3%, respectively, after treatment with HP-S exudates. In further investigation of the time course of NIH-3T3 cells treated with 50 microg/mL H. pylori, the exudates revealed that the proliferation rate was elevated 14%, 23.7%, 38.7%, 31.6%, and 29%, respectively, after HP-W treatment and elevated 29.8%, 50.4%, 78.5%, 62.3%, and 55.9% after HP-S treatment for 6, 12, 24, 48, and 72 h, respectively. In conclusion, membrane array promises a new diagnostic tool to detect H. pylori more sensitively than the CLO test. These results suggest that urease may play an important role in the development of gastric mucosal hyperproliferation in H. pylori-induced gastritis.

Acta dermato-venereologica, 2006

Toxic epidermal necrolysis and Stevens-Johnson syndrome are severe blistering diseases generally ... more Toxic epidermal necrolysis and Stevens-Johnson syndrome are severe blistering diseases generally considered to be hypersensitivity reaction to drugs. The aim of this study is to explore the diagnostic role of soluble Fas ligand secretion by peripheral blood mononuclear cells (PBMCs) in patients with previous drug-induced blistering diseases with or without stimulation with the offending drug. The results revealed that PBMCs from the patient group showed significant soluble Fas ligand secretion after stimulation with 100 μg/ml culprit drug (p<0.05). No significant change was seen in the control groups (p>0.05). Taken as a group, PBMCs from the patient group secreted more soluble Fas ligand than the normal control group regardless of drug stimulation. These results indicate that individuals whose PBMCs are high secretors of soluble Fas ligand are prone to severe toxic epidermal necrolysis/Stevens-Johnson syndrome. The possibility of using soluble Fas ligand secretion as a tool for retrospective determination of culprit drugs in patients with previous drug-induced blistering disease awaits further investigation.

Journal of The European Academy of Dermatology and Venereology, May 4, 2011

Background The pathogenesis of vitiligo remains unclear. Most authorities favoured the autoimmune... more Background The pathogenesis of vitiligo remains unclear. Most authorities favoured the autoimmune cause for the strong associations of vitiligo with multiple autoimmune diseases and the presence of autoantibodies in vitiligo patients. Narrow-band UVB (NBUVB) irradiation has been considered to be an effective treatment for vitiligo with simple treatment procedure and decreased accumulated ultraviolet exposure doses. Objectives The aim this study was to investigate the effects of NBUVB irradiation on normal IgG antibodies (N-IgG) or vitiligo IgG antibodies (V-IgG)-treated NCCmelan5 cells in terms of proliferation, migration and melanin formation. Methods Cultured NCCmelan5 cells were treated with (i) NBUVB irradiation alone, (ii) N-IgG or V-IgG alone, and (iii) combination of N-IgG or V-IgG with NBUVB irradiation. The proliferation of NCCmelan5 cells were evaluated using BrdU incorporation assay. Western blotting was used to determine the expressions of phosphorylated p125 FAK (pp125 FAK) and tyrosinase in NCCmelan5 cells. The locomotion of NCCmelan5 cells was assessed using time-lapse assay and in vitro wound scratch assay. Results Neither N-IgG nor V-IgG significantly affected the proliferation of NCCmelan5 cells. The migration, melanin formation and tyrosinase expression in NCCmelan5 cells were decreased by V-IgG. NBUVB irradiation increased the proliferation of V-IgG treated NCCmelan5 cells. In addition, NBUVB irradiation enhanced the mobility of V-IgGtreated NCCmelan5 cells via upregulation of pp125 FAK. The melanogenesis and tyrosinase expression in V-IgGtreated NCCmelan5 cells were promoted using NBUVB irradiation. Conclusions Our study demonstrated that the deleterious effects of V-IgG in the pathogenesis of vitiligo might be overcome by NBUVB irradiation.