Chris huang - Academia.edu (original) (raw)
Papers by Chris huang
polymorphism, on the gene expression of human airway smooth
Regulation of Heat Shock Protein Responses, 2018
La presente invention concerne l'amelioration de la selectivite de gravure lors d'une gra... more La presente invention concerne l'amelioration de la selectivite de gravure lors d'une gravure chimique activee par faisceau electronique, des procedes et un appareil permettant d'evaluer la qualite des structures sur une tranche a circuit integre mettant en oeuvre la gravure chimique activee par faisceau electronique, un procede de modification d'une surface d'un substrat (ou une partie de celle-ci), des procedes et un appareil pour l'imagerie d'une structure et un support lisible par processeur associe. Une cible ou une partie de celle-ci peut etre exposee a une composition gazeuse du type qui realise la gravure de la cible lorsque la composition gazeuse et/ou la cible sont exposees a un faisceau electronique. Grâce a l'orientation d'un faisceau electronique en direction de la cible dans le voisinage de la composition gazeuse, une interaction entre le faisceau electronique est la composition gazeuse realise la gravure d'une partie de la cible...
mBio, 2021
Understanding the mechanisms which regulate HCMV latency could allow therapeutic targeting of the... more Understanding the mechanisms which regulate HCMV latency could allow therapeutic targeting of the latent virus reservoir from where virus reactivation can cause severe disease. We show that the BMPR2/TGFbeta receptor/YY1 signaling axis is crucial to maintain HCMV latency in undifferentiated cells and that pharmacological reduction of BMPR2 in latently infected cells leads to reactivation of the viral lytic transcription program, which renders the infected cell open to immune detection and clearance in infected individuals.
BMC complementary and alternative medicine, Jan 2, 2017
Indigo naturalis is a Traditional Chinese Medicine (TCM) ingredient long-recognized as a therapy ... more Indigo naturalis is a Traditional Chinese Medicine (TCM) ingredient long-recognized as a therapy for several inflammatory conditions, including psoriasis. However, its mechanism is unknown due to lack of knowledge about the responsible chemical entity. We took a different approach to this challenge by investigating the molecular profile of Indigo naturalis treatment and impacted pathways. A randomized, double-blind, placebo-controlled clinical study was conducted using Indigo naturalis as topical monotherapy to treat moderate plaque psoriasis in a Chinese cohort (n = 24). Patients were treated with Indigo naturalis ointment (n = 16) or matched placebo (n = 8) twice daily for 8 weeks, with 1 week of follow-up. At week 8, significant improvements in Psoriasis Area and Severity Index (PASI) scores from baseline were observed in Indigo naturalis-treated patients (56.3% had 75% improvement [PASI 75] response) compared with placebo (0.0%). A gene expression signature of moderate psoriasis...
The invention discloses a spinal dynamic stabilization device (10) for relieving low back pain th... more The invention discloses a spinal dynamic stabilization device (10) for relieving low back pain through increasing foramen route of spine stenosis patients. Each vertebra includes a spinous process and symmetric pedicles. The spinal dynamic stabilization device (10) includes a main body (11), a foldable supporting structure (12) and a sliding bar (13). The supporting structure comprises two support portions (121, 122), when the sliding bar approaches the main body, the support portions rotatably expand toward the adjacent vertebrae of the spine.
The invention includes an isolated nucleic acid encoding at least one CNGH0004 polypeptide or ant... more The invention includes an isolated nucleic acid encoding at least one CNGH0004 polypeptide or antibody, at least one novel CNGH0004 polypeptides, antibodies, CNGH0004 vectors, host cells, transgenic animals or plants, and their It relates to a method of making and using, including therapeutic compositions, methods and devices.
Journal of Traditional Chinese Medicine, 2015
BMC bioinformatics, Jan 22, 2015
Next-generation sequencing (NGS) technologies have become much more efficient, allowing whole hum... more Next-generation sequencing (NGS) technologies have become much more efficient, allowing whole human genomes to be sequenced faster and cheaper than ever before. However, processing the raw sequence reads associated with NGS technologies requires care and sophistication in order to draw compelling inferences about phenotypic consequences of variation in human genomes. It has been shown that different approaches to variant calling from NGS data can lead to different conclusions. Ensuring appropriate accuracy and quality in variant calling can come at a computational cost. We describe our experience implementing and evaluating a group-based approach to calling variants on large numbers of whole human genomes. We explore the influence of many factors that may impact the accuracy and efficiency of group-based variant calling, including group size, the biogeographical backgrounds of the individuals who have been sequenced, and the computing environment used. We make efficient use of the G...
American journal of orthopedics (Belle Mead, N.J.), 2015
Unstable proximal interphalangeal (PIP) joint fracture-dislocations, which can cause significant ... more Unstable proximal interphalangeal (PIP) joint fracture-dislocations, which can cause significant disability, can be treated with multiple techniques. Extension-block pinning (EBP) allows for early motion and is less technically demanding than alternative surgical treatments. In the study reported here, 12 patients with unstable dorsal PIP fracture-dislocations were treated with closed reduction of the PIP joint followed by percutaneous insertion of a Kirschner wire (K-wire) into the distal aspect of the proximal phalanx. For these patients, extent of articular surface involvement averaged 43% (range, 25%-75%). Active motion was initiated early after surgery, and the K-wire was removed a mean of 25 days after pinning. Radiographic reduction of joint dislocation was achieved and maintained for 11 of the 12 patients at a mean follow-up of 35.5 months. At follow-up, mean visual analog scale (VAS) score was 0.64 (scale, 0-10). Mean score on the Quick Disabilities of the Arm, Shoulder, an...
Zhonghua yi xue za zhi, 1990
Superoxide dismutase (SOD) activity in hepatocellular carcinoma (HCC) tissue was studied. It was ... more Superoxide dismutase (SOD) activity in hepatocellular carcinoma (HCC) tissue was studied. It was observed that activities of total SOD, Cu, Zn-SOD and Mn-SOD in HCC tissue were lower than those in normal liver tissues respectively (P less than 0.001 & 0.01 less than P less than 0.05). SOD activity in poorly differentiated HCC tissue was lower than that in well differentiated HCC tissue. Contents of copper, zinc and manganese in HCC tissues were lower than those in normal liver tissues respectively (P less than 0.001 & P less than 0.01). This study suggests that decreased content of copper, zinc and manganese may be one of the factors that lead to impairment of SOD activity. The characteristic of lower SOD activity in HCC tissue and poorly differentiated HCC tissue may be a negative regulation to limitless proliferation and poor differentiation of liver cancer cells.
Muscle & Nerve, 2004
Partial nerve lesions with a varying degree of retained function and often a painful neuroma pose... more Partial nerve lesions with a varying degree of retained function and often a painful neuroma pose a dilemma for the clinician. Surgical treatment of partial nerve lesion is perilous because of possible damage to intact axons and subsequent loss of retained function. We present a new rat model of a partial nerve lesion, allowing further study to improve treatment for this condition. A partial (50%) lesion of the tibial portion of the rat sciatic nerve was created and compared to standard crush and neurectomy control lesions. The extent of lost function and the progress of postoperative recovery following the three lesions were compared using serial walking track analyses and end-point muscle weight ratios for atrophy as outcome measures. All groups had tibial functional indices (TFI) significantly different from one another after 1 week. TFIs for the crush group returned to normal by 4 weeks, whereas the neurectomy group showed no recovery. The partial lesion group gradually improved, reaching a plateau of 44% by 7 weeks. Gastrocnemius muscle weight ratios for the partial, crush, and neurectomy lesions at 9 weeks were 0.63, 0.87, and 0.32, respectively. There was a strong correlation between the TFI and muscle weight ratios (r(2) = 0.89; P < 0.001) suggesting that these outcome measures are highly predictive of function. In conclusion, the partial lesion showed a gradual but incomplete functional recovery with a complementary degree of muscle atrophy. The model may prove useful in the evaluation of proposed treatments for partial nerve lesions and the associated painful state.
Molecular and Cellular Biology, 2006
Class IIa histone deacetylases (HDACs) are found both in the cytoplasm and in the nucleus where t... more Class IIa histone deacetylases (HDACs) are found both in the cytoplasm and in the nucleus where they repress genes involved in several major developmental programs. In response to specific signals, the repressive activity of class IIa HDACs is neutralized through their phosphorylation on multiple N-terminal serine residues and 14-3-3-mediated nuclear exclusion. Here, we demonstrate that class IIa HDACs are subjected to signal-independent nuclear export that relies on their constitutive phosphorylation. We identify EMK and C-TAK1, two members of the microtubule affinity-regulating kinase (MARK)/Par-1 family, as regulators of this process. We further show that EMK and C-TAK1 phosphorylate class IIa HDACs on one of their multiple 14-3-3 binding sites and alter their subcellular localization and repressive function. Using HDAC7 as a paradigm, we extend these findings by demonstrating that signal-independent phosphorylation of the most N-terminal serine residue by the MARK/Par-1 kinases,...
Journal of Pharmacology and Experimental Therapeutics, 2005
Journal of Investigative Dermatology, 2007
Studies performed to discover genes overexpressed in inflammatory diseases identified dermokine a... more Studies performed to discover genes overexpressed in inflammatory diseases identified dermokine as being upregulated in such disease conditions. Dermokine is a gene that was first observed as expressed in the differentiated layers of skin. Its two major isoforms, alpha and beta, are transcribed from different promoters of the same locus, with the alpha isoform representing the C terminus of the beta isoform. Recently, additional transcript variants have been identified. Extensive in silico analysis and reverse transcriptase (RT)-PCR cloning has confirmed the existence of these variants in human cells and tissues, identified a new human isoform as well as the gamma isoform in mouse. Recombinant expression and analysis of the C-terminal truncated isoform indicate that the molecule is O-linked glycosylated and forms multimers in solution. In situ hybridization and immunohistochemistry has shown that the gene is differentially expressed in various cells and tissues, other than the skin. These results show that the dermokine gene is expressed in epithelial tissues other than the skin and this expression is transcriptionally and posttranscriptionally complex.
Corrosion, 2009
This paper analyzes, using the theory of critical distances, the environmentally assisted crackin... more This paper analyzes, using the theory of critical distances, the environmentally assisted cracking behavior of two steels (S420 and API X80) subjected to two different aggressive environments. The propagation threshold for environmentally assisted cracking (i.e., the stress intensity factor above which crack propagation initiates) in cracked and notched specimens (K IEAC and K N IEAC) has been experimentally obtained under different environmental conditions. Cathodic polarization has been employed to generate the aggressive environments, at 1 and 5 mA/cm 2 , causing hydrogen embrittlement on the steels. The point method and the line method, both belonging to the theory of critical distances, have been applied to verify their capacity to predict the initiation of crack propagation. The results demonstrate the capacity of the theory of critical distances to predict the crack propagation onset under the different combinations of material and aggressive environments.
polymorphism, on the gene expression of human airway smooth
Regulation of Heat Shock Protein Responses, 2018
La presente invention concerne l'amelioration de la selectivite de gravure lors d'une gra... more La presente invention concerne l'amelioration de la selectivite de gravure lors d'une gravure chimique activee par faisceau electronique, des procedes et un appareil permettant d'evaluer la qualite des structures sur une tranche a circuit integre mettant en oeuvre la gravure chimique activee par faisceau electronique, un procede de modification d'une surface d'un substrat (ou une partie de celle-ci), des procedes et un appareil pour l'imagerie d'une structure et un support lisible par processeur associe. Une cible ou une partie de celle-ci peut etre exposee a une composition gazeuse du type qui realise la gravure de la cible lorsque la composition gazeuse et/ou la cible sont exposees a un faisceau electronique. Grâce a l'orientation d'un faisceau electronique en direction de la cible dans le voisinage de la composition gazeuse, une interaction entre le faisceau electronique est la composition gazeuse realise la gravure d'une partie de la cible...
mBio, 2021
Understanding the mechanisms which regulate HCMV latency could allow therapeutic targeting of the... more Understanding the mechanisms which regulate HCMV latency could allow therapeutic targeting of the latent virus reservoir from where virus reactivation can cause severe disease. We show that the BMPR2/TGFbeta receptor/YY1 signaling axis is crucial to maintain HCMV latency in undifferentiated cells and that pharmacological reduction of BMPR2 in latently infected cells leads to reactivation of the viral lytic transcription program, which renders the infected cell open to immune detection and clearance in infected individuals.
BMC complementary and alternative medicine, Jan 2, 2017
Indigo naturalis is a Traditional Chinese Medicine (TCM) ingredient long-recognized as a therapy ... more Indigo naturalis is a Traditional Chinese Medicine (TCM) ingredient long-recognized as a therapy for several inflammatory conditions, including psoriasis. However, its mechanism is unknown due to lack of knowledge about the responsible chemical entity. We took a different approach to this challenge by investigating the molecular profile of Indigo naturalis treatment and impacted pathways. A randomized, double-blind, placebo-controlled clinical study was conducted using Indigo naturalis as topical monotherapy to treat moderate plaque psoriasis in a Chinese cohort (n = 24). Patients were treated with Indigo naturalis ointment (n = 16) or matched placebo (n = 8) twice daily for 8 weeks, with 1 week of follow-up. At week 8, significant improvements in Psoriasis Area and Severity Index (PASI) scores from baseline were observed in Indigo naturalis-treated patients (56.3% had 75% improvement [PASI 75] response) compared with placebo (0.0%). A gene expression signature of moderate psoriasis...
The invention discloses a spinal dynamic stabilization device (10) for relieving low back pain th... more The invention discloses a spinal dynamic stabilization device (10) for relieving low back pain through increasing foramen route of spine stenosis patients. Each vertebra includes a spinous process and symmetric pedicles. The spinal dynamic stabilization device (10) includes a main body (11), a foldable supporting structure (12) and a sliding bar (13). The supporting structure comprises two support portions (121, 122), when the sliding bar approaches the main body, the support portions rotatably expand toward the adjacent vertebrae of the spine.
The invention includes an isolated nucleic acid encoding at least one CNGH0004 polypeptide or ant... more The invention includes an isolated nucleic acid encoding at least one CNGH0004 polypeptide or antibody, at least one novel CNGH0004 polypeptides, antibodies, CNGH0004 vectors, host cells, transgenic animals or plants, and their It relates to a method of making and using, including therapeutic compositions, methods and devices.
Journal of Traditional Chinese Medicine, 2015
BMC bioinformatics, Jan 22, 2015
Next-generation sequencing (NGS) technologies have become much more efficient, allowing whole hum... more Next-generation sequencing (NGS) technologies have become much more efficient, allowing whole human genomes to be sequenced faster and cheaper than ever before. However, processing the raw sequence reads associated with NGS technologies requires care and sophistication in order to draw compelling inferences about phenotypic consequences of variation in human genomes. It has been shown that different approaches to variant calling from NGS data can lead to different conclusions. Ensuring appropriate accuracy and quality in variant calling can come at a computational cost. We describe our experience implementing and evaluating a group-based approach to calling variants on large numbers of whole human genomes. We explore the influence of many factors that may impact the accuracy and efficiency of group-based variant calling, including group size, the biogeographical backgrounds of the individuals who have been sequenced, and the computing environment used. We make efficient use of the G...
American journal of orthopedics (Belle Mead, N.J.), 2015
Unstable proximal interphalangeal (PIP) joint fracture-dislocations, which can cause significant ... more Unstable proximal interphalangeal (PIP) joint fracture-dislocations, which can cause significant disability, can be treated with multiple techniques. Extension-block pinning (EBP) allows for early motion and is less technically demanding than alternative surgical treatments. In the study reported here, 12 patients with unstable dorsal PIP fracture-dislocations were treated with closed reduction of the PIP joint followed by percutaneous insertion of a Kirschner wire (K-wire) into the distal aspect of the proximal phalanx. For these patients, extent of articular surface involvement averaged 43% (range, 25%-75%). Active motion was initiated early after surgery, and the K-wire was removed a mean of 25 days after pinning. Radiographic reduction of joint dislocation was achieved and maintained for 11 of the 12 patients at a mean follow-up of 35.5 months. At follow-up, mean visual analog scale (VAS) score was 0.64 (scale, 0-10). Mean score on the Quick Disabilities of the Arm, Shoulder, an...
Zhonghua yi xue za zhi, 1990
Superoxide dismutase (SOD) activity in hepatocellular carcinoma (HCC) tissue was studied. It was ... more Superoxide dismutase (SOD) activity in hepatocellular carcinoma (HCC) tissue was studied. It was observed that activities of total SOD, Cu, Zn-SOD and Mn-SOD in HCC tissue were lower than those in normal liver tissues respectively (P less than 0.001 & 0.01 less than P less than 0.05). SOD activity in poorly differentiated HCC tissue was lower than that in well differentiated HCC tissue. Contents of copper, zinc and manganese in HCC tissues were lower than those in normal liver tissues respectively (P less than 0.001 & P less than 0.01). This study suggests that decreased content of copper, zinc and manganese may be one of the factors that lead to impairment of SOD activity. The characteristic of lower SOD activity in HCC tissue and poorly differentiated HCC tissue may be a negative regulation to limitless proliferation and poor differentiation of liver cancer cells.
Muscle & Nerve, 2004
Partial nerve lesions with a varying degree of retained function and often a painful neuroma pose... more Partial nerve lesions with a varying degree of retained function and often a painful neuroma pose a dilemma for the clinician. Surgical treatment of partial nerve lesion is perilous because of possible damage to intact axons and subsequent loss of retained function. We present a new rat model of a partial nerve lesion, allowing further study to improve treatment for this condition. A partial (50%) lesion of the tibial portion of the rat sciatic nerve was created and compared to standard crush and neurectomy control lesions. The extent of lost function and the progress of postoperative recovery following the three lesions were compared using serial walking track analyses and end-point muscle weight ratios for atrophy as outcome measures. All groups had tibial functional indices (TFI) significantly different from one another after 1 week. TFIs for the crush group returned to normal by 4 weeks, whereas the neurectomy group showed no recovery. The partial lesion group gradually improved, reaching a plateau of 44% by 7 weeks. Gastrocnemius muscle weight ratios for the partial, crush, and neurectomy lesions at 9 weeks were 0.63, 0.87, and 0.32, respectively. There was a strong correlation between the TFI and muscle weight ratios (r(2) = 0.89; P < 0.001) suggesting that these outcome measures are highly predictive of function. In conclusion, the partial lesion showed a gradual but incomplete functional recovery with a complementary degree of muscle atrophy. The model may prove useful in the evaluation of proposed treatments for partial nerve lesions and the associated painful state.
Molecular and Cellular Biology, 2006
Class IIa histone deacetylases (HDACs) are found both in the cytoplasm and in the nucleus where t... more Class IIa histone deacetylases (HDACs) are found both in the cytoplasm and in the nucleus where they repress genes involved in several major developmental programs. In response to specific signals, the repressive activity of class IIa HDACs is neutralized through their phosphorylation on multiple N-terminal serine residues and 14-3-3-mediated nuclear exclusion. Here, we demonstrate that class IIa HDACs are subjected to signal-independent nuclear export that relies on their constitutive phosphorylation. We identify EMK and C-TAK1, two members of the microtubule affinity-regulating kinase (MARK)/Par-1 family, as regulators of this process. We further show that EMK and C-TAK1 phosphorylate class IIa HDACs on one of their multiple 14-3-3 binding sites and alter their subcellular localization and repressive function. Using HDAC7 as a paradigm, we extend these findings by demonstrating that signal-independent phosphorylation of the most N-terminal serine residue by the MARK/Par-1 kinases,...
Journal of Pharmacology and Experimental Therapeutics, 2005
Journal of Investigative Dermatology, 2007
Studies performed to discover genes overexpressed in inflammatory diseases identified dermokine a... more Studies performed to discover genes overexpressed in inflammatory diseases identified dermokine as being upregulated in such disease conditions. Dermokine is a gene that was first observed as expressed in the differentiated layers of skin. Its two major isoforms, alpha and beta, are transcribed from different promoters of the same locus, with the alpha isoform representing the C terminus of the beta isoform. Recently, additional transcript variants have been identified. Extensive in silico analysis and reverse transcriptase (RT)-PCR cloning has confirmed the existence of these variants in human cells and tissues, identified a new human isoform as well as the gamma isoform in mouse. Recombinant expression and analysis of the C-terminal truncated isoform indicate that the molecule is O-linked glycosylated and forms multimers in solution. In situ hybridization and immunohistochemistry has shown that the gene is differentially expressed in various cells and tissues, other than the skin. These results show that the dermokine gene is expressed in epithelial tissues other than the skin and this expression is transcriptionally and posttranscriptionally complex.
Corrosion, 2009
This paper analyzes, using the theory of critical distances, the environmentally assisted crackin... more This paper analyzes, using the theory of critical distances, the environmentally assisted cracking behavior of two steels (S420 and API X80) subjected to two different aggressive environments. The propagation threshold for environmentally assisted cracking (i.e., the stress intensity factor above which crack propagation initiates) in cracked and notched specimens (K IEAC and K N IEAC) has been experimentally obtained under different environmental conditions. Cathodic polarization has been employed to generate the aggressive environments, at 1 and 5 mA/cm 2 , causing hydrogen embrittlement on the steels. The point method and the line method, both belonging to the theory of critical distances, have been applied to verify their capacity to predict the initiation of crack propagation. The results demonstrate the capacity of the theory of critical distances to predict the crack propagation onset under the different combinations of material and aggressive environments.