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Papers by Christina Wennerberg
IFAC Proceedings Volumes, Mar 1, 2004
Differences and similarities in enzymes from the neopullulanase subfamily isolated from thermophi... more Differences and similarities in enzymes from the neopullulanase subfamily isolated from thermophilic species
IFAC Proceedings Volumes, 2004
Green Chem., 2010
The antioxidant quercetin was extracted from yellow onion waste and converted to its aglycone for... more The antioxidant quercetin was extracted from yellow onion waste and converted to its aglycone form by a combination of subcritical water extraction and enzymatic hydrolysis. The hydrolytic step was catalysed by a double residue (N221S, P342L) mutant of the thermostable β-...
Biologia, 2008
Six glycoside hydrolase (GH) family 13 members, classified under the polyspecific neopullulanase ... more Six glycoside hydrolase (GH) family 13 members, classified under the polyspecific neopullulanase subfamily GH13_20 (also termed cyclomaltodextrinase) were analysed. They originate from thermophilic bacterial strains (Anoxybacillus flavithermus, Laceyella sacchari, and Geobacillus thermoleovorans) or from environmental DNA, collected after in situ enrichments in Icelandic hot springs. The genes were isolated following the CODEHOP consensus primer strategy, utilizing the first two of the four conserved sequence regions in GH13. The typical domain structure of GH13_20, including an N-terminal domain (classified as CBM34), the catalytic module composed of the A-and B-domains, and a C-terminal domain, was found in five of the encoded enzymes (abbreviated Amy1, 89, 92, 98 and 132). These five enzymes degraded cyclomaltodextrins (CDs) and starch, while only three, Amy92 (L. sacchari), Amy98 (A. flavithermus) and Amy132 (environmental DNA), also harboured neopullulanase activity. The L. sac...
IFAC Proceedings Volumes, Mar 1, 2004
Differences and similarities in enzymes from the neopullulanase subfamily isolated from thermophi... more Differences and similarities in enzymes from the neopullulanase subfamily isolated from thermophilic species
IFAC Proceedings Volumes, 2004
Green Chem., 2010
The antioxidant quercetin was extracted from yellow onion waste and converted to its aglycone for... more The antioxidant quercetin was extracted from yellow onion waste and converted to its aglycone form by a combination of subcritical water extraction and enzymatic hydrolysis. The hydrolytic step was catalysed by a double residue (N221S, P342L) mutant of the thermostable β-...
Biologia, 2008
Six glycoside hydrolase (GH) family 13 members, classified under the polyspecific neopullulanase ... more Six glycoside hydrolase (GH) family 13 members, classified under the polyspecific neopullulanase subfamily GH13_20 (also termed cyclomaltodextrinase) were analysed. They originate from thermophilic bacterial strains (Anoxybacillus flavithermus, Laceyella sacchari, and Geobacillus thermoleovorans) or from environmental DNA, collected after in situ enrichments in Icelandic hot springs. The genes were isolated following the CODEHOP consensus primer strategy, utilizing the first two of the four conserved sequence regions in GH13. The typical domain structure of GH13_20, including an N-terminal domain (classified as CBM34), the catalytic module composed of the A-and B-domains, and a C-terminal domain, was found in five of the encoded enzymes (abbreviated Amy1, 89, 92, 98 and 132). These five enzymes degraded cyclomaltodextrins (CDs) and starch, while only three, Amy92 (L. sacchari), Amy98 (A. flavithermus) and Amy132 (environmental DNA), also harboured neopullulanase activity. The L. sac...