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Papers by Christine Fleischacker

Journal of Immunology, Feb 15, 1993

Class I-restricted presentation occurs without internalization or processing of exogenous antigen... more Class I-restricted presentation occurs without internalization or processing of exogenous antigenic peptides.

The Journal of Immunology

Ag in the extracellular fluids can be internalized, processed, and presented in association with ... more Ag in the extracellular fluids can be internalized, processed, and presented in association with class I MHC molecules on specialized APC in normal spleen. We examine the fate of these APC after they present Ag to a CTL. When splenocytes present exogenous OVA to CTL, their ability to subsequently present native Ag in association with both class I and class II molecules is inhibited. CTL do not inhibit the ability of splenocytes to present processing independent peptides with class I or class II molecules. Inhibition of Ag presentation is only observed in the presence of the specific Ag recognized by the CTL. This inhibition is MHC-restricted. In the presence of specific Ag, CTL inhibit the ability of APC to present unrelated Ag. However, bystander APC are not affected by activated CTL. Taken together these results indicate that when APC present exogenous Ag to CTL, they are inhibited or killed. The CTL that mediates this activity has a conventional CD4-CD8+ phenotype and utilizes a ...

CBE—Life Sciences Education, 2022

A report on research that explicates three models of pedagogical practice that underpin and chara... more A report on research that explicates three models of pedagogical practice that underpin and characterize inquiry instruction in a course-based research experience.

Proceedings of the National Academy of Sciences of the United States of America, Dec 19, 2017

Engaging undergraduate students in scientific research promises substantial benefits, but it is n... more Engaging undergraduate students in scientific research promises substantial benefits, but it is not accessible to all students and is rarely implemented early in college education, when it will have the greatest impact. An inclusive Research Education Community (iREC) provides a centralized scientific and administrative infrastructure enabling engagement of large numbers of students at different types of institutions. The Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) is an iREC that promotes engagement and continued involvement in science among beginning undergraduate students. The SEA-PHAGES students show strong gains correlated with persistence relative to those in traditional laboratory courses regardless of academic, ethnic, gender, and socioeconomic profiles. This persistent involvement in science is reflected in key measures, including project ownership, scientific community values, science identity, and scientific networking.

Genome announcements, Jan 13, 2017

Mycobacteriophage CrystalP is a newly isolated phage infecting Mycobacterium smegmatis strain mc(... more Mycobacteriophage CrystalP is a newly isolated phage infecting Mycobacterium smegmatis strain mc(2)155. CrystalP has a 76,483-bp genome and is predicted to contain 143 protein-coding and 2 tRNA genes, including repressor and integrase genes consistent with a temperate lifestyle. CrystalP is related to the mycobacteriophages Toto and Kostya and to other Cluster E phages.

The Journal of Immunology, 2001

Immunological Reviews, 2006

The generation of CD4 and CD8 alphabeta T-cell lineages from CD4+ CD8+ double-positive (DP) thymo... more The generation of CD4 and CD8 alphabeta T-cell lineages from CD4+ CD8+ double-positive (DP) thymocyte precursors is a complex process initiated by engagement of major histocompatibility complex (MHC) by T-cell receptor (TCR) and coreceptor. Quantitative differences in TCR signaling induced by this interaction impose an instructional bias on CD4/CD8 lineage commitment that must be reinforced by MHC recognition and TCR signaling over subsequent selection steps in order for the thymocyte to progress and mature in the adopted lineage. Our studies show that the transmembrane receptor Notch plays a role in this process by modifying TCR signal transduction in DP thymocytes. In this review, we consider the functional relationship of TCR and Notch signaling pathways in the selection and specification of CD4 and CD8 T-cell lineages.

Immunity, 2006

Mutations affecting the Tec kinases Itk and Rlk decrease T cell receptor-induced Ca 2+ mobilizati... more Mutations affecting the Tec kinases Itk and Rlk decrease T cell receptor-induced Ca 2+ mobilization and Erk kinase activation and impair both positive and negative thymic selection. Itk 2/2 and Rlk 2/2 Itk 2/2 mice also have decreased CD4:8 T cell ratios, suggestive of altered CD4:8 lineage commitment. Nonetheless, we find that CD8 single-positive (SP) thymocytes and peripheral CD8 + T cells in these mice do not resemble conventional CD8 + T cells. Instead, these cells express memory markers, rapidly produce interferong, and can be selected on hematopoietically derived cells, similar to MHC class Ib-restricted ''innate-type'' lymphocytes. Itk deficiency also greatly increases the number of cells selected by MHC class Ib. Expression of a hypersensitive Erk2 mutant partially corrects the CD8 + T cell phenotypes in Itk 2/2 mice, arguing that altered signaling permits development of this innatetype CD8 + cell population. Our results suggest that Tec kinases differentially regulate development of conventional versus nonconventional lymphocytes.