Christopher Brenden - Academia.edu (original) (raw)

Christopher Brenden

Address: Albuquerque, New Mexico, United States

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Papers by Christopher Brenden

Research paper thumbnail of Highly Localized Chemical Sampling at Subsecond Temporal Resolution Enabled with a Silicon Nanodialysis Platform at Nanoliter per Minute Flows

Research paper thumbnail of Highly localized chemical sampling at sub-second temporal resolution enabled with a silicon nanodialysis platform at exceedingly slow flows

bioRxiv (Cold Spring Harbor Laboratory), Sep 11, 2023

Microdialysis (MD) is a versatile and powerful technique for chemical profiling of biological tis... more Microdialysis (MD) is a versatile and powerful technique for chemical profiling of biological tissues and is widely used for quantification of neurotransmitters, neuropeptides, metabolites, biomarkers, and drugs in the central nervous system as well as in dermatology, ophthalmology, and in pain research. However, MD performance is severely limited by fundamental tradeoffs between chemical sensitivity, spatial resolution, and temporal response. Here, by using wafer-scale silicon microfabrication, we develop and demonstrate a nanodialysis (ND) sampling probe that enables highly localized chemical sampling with 100µm spatial resolution and sub-second temporal resolution at high recovery rates. These performance metrics, which are 100X-1000X superior to existing MD approaches, are enabled by a 100X reduction of the microfluidic channel cross-section, a corresponding drastic 100X reduction of flow rates to exceedingly slow few nL/min flows, and integration of a nanometer-thin nanoporous membrane with high transport flux into the probe sampling area. Miniaturized ND probes may allow for the minimally invasive and highly localized sampling and chemical profiling in live biological tissues with unprecedented spatio-temporal resolution for clinical, biomedical, and pharmaceutical applications.

Research paper thumbnail of Enhancement of faradaic current in an electrochemical cell integrated into silicon microfluidic channels

Sensors and Actuators B-chemical, Jun 1, 2023

Research paper thumbnail of Attomole-Level Multiplexed Detection of Neurochemicals in Picoliter Droplets by On-Chip Nanoelectrospray Ionization Coupled to Mass Spectrometry

Analytical Chemistry, Sep 27, 2022

Research paper thumbnail of Enhancement of faradaic current in an electrochemical cell integrated into silicon microfluidic channels

Sensors and Actuators B: Chemical

Research paper thumbnail of Integrated silicon microfluidic chip for picoliter-scale analyte segmentation and microscale printing for mass spectrometry imaging

Lab on a Chip

A silicon integrated microfluidics system prints picoliter-segmented analytes for attomole-level ... more A silicon integrated microfluidics system prints picoliter-segmented analytes for attomole-level chemical analysis with mass spectrometry imaging.

Research paper thumbnail of Lignin valorization to a water-soluble polyacid with comparable industrial dispersant performance

Research paper thumbnail of Droplet-assisted phase separation by integrated silicon electrospray nano-emitter for neurochemical sensing

24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020, 2020

Research paper thumbnail of Attomole-Level Multiplexed Detection of Neurochemicals in Picoliter Droplets by On-Chip Nanoelectrospray Ionization Coupled to Mass Spectrometry

Research paper thumbnail of Droplet-assisted electrospray phase separation using an integrated silicon microfluidic platform

Lab on a Chip, 2021

We report a silicon microfluidic platform that enables monolithic integration of transparent micr... more We report a silicon microfluidic platform that enables monolithic integration of transparent micron-scale microfluidic channels, an on-chip segmentation of analyte flows into picoliter-volume droplets, and a nano-electrospray ionization emitter that enables spatial and temporal separation of oil and aqueous phases during electro-spray for subsequent mass spectrometry analysis.

Research paper thumbnail of Efficient conversion of lignin into a water-soluble polymer by a chelator-mediated Fenton reaction: optimization of H2O2 use and performance as a dispersant

Green Chemistry

Transforming lignin into a water-soluble polymer.

Research paper thumbnail of Highly Localized Chemical Sampling at Subsecond Temporal Resolution Enabled with a Silicon Nanodialysis Platform at Nanoliter per Minute Flows

Research paper thumbnail of Highly localized chemical sampling at sub-second temporal resolution enabled with a silicon nanodialysis platform at exceedingly slow flows

bioRxiv (Cold Spring Harbor Laboratory), Sep 11, 2023

Microdialysis (MD) is a versatile and powerful technique for chemical profiling of biological tis... more Microdialysis (MD) is a versatile and powerful technique for chemical profiling of biological tissues and is widely used for quantification of neurotransmitters, neuropeptides, metabolites, biomarkers, and drugs in the central nervous system as well as in dermatology, ophthalmology, and in pain research. However, MD performance is severely limited by fundamental tradeoffs between chemical sensitivity, spatial resolution, and temporal response. Here, by using wafer-scale silicon microfabrication, we develop and demonstrate a nanodialysis (ND) sampling probe that enables highly localized chemical sampling with 100µm spatial resolution and sub-second temporal resolution at high recovery rates. These performance metrics, which are 100X-1000X superior to existing MD approaches, are enabled by a 100X reduction of the microfluidic channel cross-section, a corresponding drastic 100X reduction of flow rates to exceedingly slow few nL/min flows, and integration of a nanometer-thin nanoporous membrane with high transport flux into the probe sampling area. Miniaturized ND probes may allow for the minimally invasive and highly localized sampling and chemical profiling in live biological tissues with unprecedented spatio-temporal resolution for clinical, biomedical, and pharmaceutical applications.

Research paper thumbnail of Enhancement of faradaic current in an electrochemical cell integrated into silicon microfluidic channels

Sensors and Actuators B-chemical, Jun 1, 2023

Research paper thumbnail of Attomole-Level Multiplexed Detection of Neurochemicals in Picoliter Droplets by On-Chip Nanoelectrospray Ionization Coupled to Mass Spectrometry

Analytical Chemistry, Sep 27, 2022

Research paper thumbnail of Enhancement of faradaic current in an electrochemical cell integrated into silicon microfluidic channels

Sensors and Actuators B: Chemical

Research paper thumbnail of Integrated silicon microfluidic chip for picoliter-scale analyte segmentation and microscale printing for mass spectrometry imaging

Lab on a Chip

A silicon integrated microfluidics system prints picoliter-segmented analytes for attomole-level ... more A silicon integrated microfluidics system prints picoliter-segmented analytes for attomole-level chemical analysis with mass spectrometry imaging.

Research paper thumbnail of Lignin valorization to a water-soluble polyacid with comparable industrial dispersant performance

Research paper thumbnail of Droplet-assisted phase separation by integrated silicon electrospray nano-emitter for neurochemical sensing

24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020, 2020

Research paper thumbnail of Attomole-Level Multiplexed Detection of Neurochemicals in Picoliter Droplets by On-Chip Nanoelectrospray Ionization Coupled to Mass Spectrometry

Research paper thumbnail of Droplet-assisted electrospray phase separation using an integrated silicon microfluidic platform

Lab on a Chip, 2021

We report a silicon microfluidic platform that enables monolithic integration of transparent micr... more We report a silicon microfluidic platform that enables monolithic integration of transparent micron-scale microfluidic channels, an on-chip segmentation of analyte flows into picoliter-volume droplets, and a nano-electrospray ionization emitter that enables spatial and temporal separation of oil and aqueous phases during electro-spray for subsequent mass spectrometry analysis.

Research paper thumbnail of Efficient conversion of lignin into a water-soluble polymer by a chelator-mediated Fenton reaction: optimization of H2O2 use and performance as a dispersant

Green Chemistry

Transforming lignin into a water-soluble polymer.

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