Chun-Yu Chuang - Academia.edu (original) (raw)

Papers by Chun-Yu Chuang

In Vitro Cellular & Developmental Biology – Animal, Aug 6, 2018

Toxicology in Vitro, Jun 1, 2017

Environmental Toxicology and Pharmacology, Oct 1, 2016

Environmental Pollution, 2022

Increasing numbers of studies have demonstrated the existence of nanoplastics (1-999 nm) in the e... more Increasing numbers of studies have demonstrated the existence of nanoplastics (1-999 nm) in the environment and commercial products, but the current technologies for detecting and quantifying nanoplastics are still developing. Herein, we present a combination of two techniques, e.g., scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (ToF-SIMS), to analyze submicron-sized plastics. A drop-casting of a 20-nL particle suspension on a Piranha solution-cleaned silicon wafer with dry ice incubation and subsequent freeze-drying was used to suppress the coffee-ring effect. SEM images were used to quantify particles, and this technique is applicable for 0.195-1.04-μm polystyrene (PS), 0.311-μm polyethylene terephthalate (PET), and 0.344-μm polyethylene (PE) at a minimum concentration of 2.49 × 109 particles/mL. ToF-SIMS could not quantify the particle number, while it could semi-quantitatively estimate number ratios of submicron PE, PET, polyvinyl chloride (PVC), and PS particles in the mixture. Analysis of submicron plastics released from three hot water-steeped teabags (respectively made of PET/PE, polylactic acid (PLA), and PET) was revisited. The SEM-derived sizes and particle numbers were comparable to those measured by a nanoparticle tracking analysis (NTA) regardless of whether or not the hydro-soluble oligomers were removed. ToF-SIMS further confirmed the number ratios of different particles from a PET/PE composite teabag leachate. This method shows potential for application in analyzing more-complex plastic particles released from food contact materials.

Japanese Journal of Applied Physics, 2021

This paper presents a new multiple-time programmable (MTP) memory cell that features an n-well as... more This paper presents a new multiple-time programmable (MTP) memory cell that features an n-well as the erasing gate and is implemented in a 16 nm FinFET technology process. It is composed of slot contacts placed beside a metal gate for lateral coupling to the floating gate, while an n-well with a floating gate laid on top of it functions as erasing terminal. With adjusted slot contact length, a programming gate (PG) coupling ratio can be designed for the optimized program, erase and read operations to best meet the needs for logic non-volatile memory array development. An increase in the PG coupling ratio provides an increasing read current and lower leakage current, which brings about an improved read window in a larger array. Good endurance test results and disturb immunity were also demonstrated on these new MPT cells.

Large-scale efforts have been persistently undertaken for medical prophylaxis and treatment of CO... more Large-scale efforts have been persistently undertaken for medical prophylaxis and treatment of COVID-19 disasters worldwide. A variety of novel viral spike protein-targeted vaccine preparations have recently been clinically distributed based on accelerated approval. We revisited the early but inconclusive clinical interest in the combination of azithromycin and zinc sulfate repurposing with safety advantages. In vitro proof of concept was provided for rapid and synergistic suppression of ACE2 expression following treatments in human airway cells, Calu-3 and H322M. The two representative ACE2-expressing human airway cells indicate the upper and lower respiratory tracts. Prophylactic and early therapeutic roles of azithromycin combined with zinc are proposed for virus cellular entry prevention potential bridging to effective antibody production.

Nanomaterials, 2020

Soluble amyloid-β oligomers (oAβ42)-induced neuronal death and inflammation response has been rec... more Soluble amyloid-β oligomers (oAβ42)-induced neuronal death and inflammation response has been recognized as one of the major causes of Alzheimer’s disease (AD). In this work, a novel strategy adopting silica-coated iron oxide stir bar (MSB)-based AD therapy system via magnetic stirring-induced capture of oAβ42 into magnetic plaques (mpAβ42) and activation of microglia on cellular plaque clearance was developed. With oAβ42 being effectively converted into mpAβ42, the neurotoxicity toward neuronal cells was thus greatly reduced. In addition to the good preservation of neurite outgrowth through the diminished uptake of oAβ42, neurons treated with oAβ42 under magnetic stirring also exhibited comparable neuron-specific protein expression to those in the absence of oAβ42. The phagocytic uptake of mpAβ42 by microglia was enhanced significantly as compared to the counterpart of oAβ42, and the M1 polarization of microglia often occurring after the uptake of oAβ42 restricted to an appreciable...

Frontiers in Pharmacology, 2017

Aerosol and Air Quality Research, 2017

Aerosol and Air Quality Research, 2015

Journal of Nuclear Science and Technology, 2015

ABSTRACT It is important to monitor operators’ mental workload during the operation phase. Physio... more ABSTRACT It is important to monitor operators’ mental workload during the operation phase. Physiological measurement approaches could record the operator's mental data continuously, and might be less interruptive on the work activities. However, these methods often require the attachment of physical sensors, which are not unobtrusive in the physical sense. Furthermore, the individual difference makes calibrating to each individual tedious and requires trained persons to use. Often high noise-to-signal ratio data are hard to analyze. Due to these factors, physiological workload measurements are hardly widely applied in practical fields. In this study, an objective, non-intrusive and performance-based mental workload predictive model was proposed with high validity (R2 = 0.51), which can be applied during the operation phrase. This model, developed based on the Rasmussen's skill-rule-knowledge framework, is comprised of two novel cognitive indices, the attention required index and uncertainty index. It can be used as the basis for establishing an early online warning system automatically. Furthermore, this model also predicts the types of error-prone tasks. This kind of information is expected to provide managers and supervisors with opportunities to intervene and improve tasks before error occurred. Finally, the predictive model proposed in this paper requires more practical application in fields to be completed.

Prenatal Diagnosis, 1998

We report on the prenatal diagnosis, genetic studies, and pathology of a case with de novo isochr... more We report on the prenatal diagnosis, genetic studies, and pathology of a case with de novo isochromosome 13q. A 31-year-old primigravida was referred for genetic counselling at 26 weeks' gestation due to the sonographic findings of intrauterine growth retardation and microcephaly. Level II ultrasonograms further demonstrated alobar holoprosencephaly, hypotelorism, polydactyly, a ventricular septal defect, and a single nostril. A diagnosis of cebocephaly was made. Genetic amniocentesis and cord blood sampling revealed translocation trisomy 13 with a de novo t(13q13q) rearrangement. Chromosomal analysis using G- and C-banding techniques and fluorescence in situ hybridization (FISH) showed an apparent monocentric isochromosome. Molecular analyses using polymorphic molecular markers showed that the rearrangement was consistent with an isochromosome of maternal chromosome 13q[46,XX,i(13)(q10)]. Necropsy confirmed cebocephaly and the prenatally detected anomalies.

Journal of Medical Genetics, 1998

Journal of Biomedical Science, 2006

Cancer Epidemiology, Biomarkers & Prevention, 2012

Background: Previous studies have reported that arsenic (As) exposure in pregnancy induces DNA da... more Background: Previous studies have reported that arsenic (As) exposure in pregnancy induces DNA damage and increased risk of fetal health and development. However, the associations between As-induced DNA damage and adverse birth outcomes in newborns have yet to be established. We aimed to elucidate the association between As exposure and DNA damages in pregnant women, and link it to the increased risks of adverse birth outcomes in newborn. Methods: A birth cohort study of 309 mother-newborn pairs was recruited during 2001-2002. We collected maternal urine samples in the third trimester of pregnancy for measurements of metabolites of inorganic arsenic (iAs) using high-performance liquid chromatography/ inductively coupled plasma mass spectrometry, and DNA damage biomarkers by liquid chromatography tenden mass spectrometer. Information of birth weight, birth length, head circumference, chest girth and Apgar score of newborns were collected to assess newborn health. Results: We found a ...

Acta Obstetricia et Gynecologica Scandinavica, 1997

Glycobiology

Nonalcoholic fatty liver disease is caused by an imbalance in lipid metabolism and immune respons... more Nonalcoholic fatty liver disease is caused by an imbalance in lipid metabolism and immune response to pose a risk factor for liver fibrosis. Recent evidence indicates that M2 macrophages secrete transforming growth factor-β1, which contributes to liver fibrosis. Galectin-12 has been demonstrated to regulate lipid metabolism and macrophage polarization. The purpose of this study is to investigate the role of galectin-12 in the development of nonalcoholic fatty liver disease and fibrosis. Liver tissue from wild-type C57BL/6 mice fed with a high-fat diet containing cholesterol and cholic acid for 4–12 weeks was used to examine galectin-12 expression and its correlation with nonalcoholic fatty liver disease. Furthermore, the effects of galectin-12 on M2 macrophages during the progression of nonalcoholic fatty liver disease were investigated by studying Kupffer cells from galectin-12 knockout mice and doxycycline-inducible Gal12−/–THP-1 cells. Ablation of galectin-12 promoted M2 polariza...

After the reconstruction of SMA ontological networks, several important hub genes were f... more After the reconstruction of SMA ontological networks, several important hub genes were further selected based on the higher connectivity with other genes. (a) In SI/C, 6 out of 20 hub genes were selected as candidate target genes, including TNFα, Bmp4, Serpine1, Ptgs2, Bcl2, and IL6. (b) In SI/SIII, 7 out of 22 hub genes were indicated as candidate target genes, including TNFα, Bmp4, Serpine1, Gata6, Ptgs2, Bcl2, and Cntn1. Among these hub genes, several of them have been indicated in SMA in other studies, including Shh [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref029" target="_blank">29</a>], Bcl2 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref030" target="_blank">30</a>], Ccl2 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref031" target="_blank">31</a>], VEGFA [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref032" target="_blank">32</a>], Prl [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref033" target="_blank">33</a>], Igf1 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref026" target="_blank">26</a>], Fos [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref034" target="_blank">34</a>], Ngf [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref035" target="_blank">35</a>], and Egf [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157426#pone.0157426.ref034" target="_blank">34</a>]. Enlarged red dots, selected candidate target genes; green arrow, activation; yellow line with red bar, inhibition; yellow line with circle, co-expression.</p