Cibele Lhamas - Academia.edu (original) (raw)
Papers by Cibele Lhamas
Cryosurgery has been used to treat different diseases of systems and organs, although adverse eff... more Cryosurgery has been used to treat different diseases of systems and organs, although adverse effects have been reported such as delayed wound healing, large scars, esthetical deformation and functional impairment. Injuries caused naturally by the exposure to extreme cold weather conditions mostly result in gangrene. This study aims to evaluate the influence of adipose-derived stem cells (ADSCs) in the proliferation phase on cutaneous wound healing. Through the application of liquid nitrogen by the spraying technique, a 15 millimeter diameter lesion was produced in the dorsal region of each rat. The wound received treatment according to the group it belonged: 1) ADSCs application on the 15th day (treated group); 2) application of 0.9% sodium chloride solution on the 15th day (sham group); 3) no intervention until euthanasia (control group). The group treated with ADSCs showed the highest wound average contraction rate; this group got a significant statistical difference in relation ...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2020
RESUMO A criocirurgia tem sido utilizada no tratamento de diferentes enfermidades de sistemas e ó... more RESUMO A criocirurgia tem sido utilizada no tratamento de diferentes enfermidades de sistemas e órgãos. Contudo, são relatados efeitos adversos, como cicatrização lenta, cicatrizes extensas, disfunção estética e funcional. As lesões que ocorrem naturalmente pela exposição ao frio extremo, comumente, resultam em gangrena. O presente trabalho teve como objetivo avaliar a influência das células-tronco mesenquimais de origem adiposa (ADSCs) na fase de proliferação da cicatrização de feridas cutâneas. Por meio da aplicação do nitrogênio líquido pela técnica do spray aberto, realizou-se a indução de uma ferida, de aproximadamente 15mm de diâmetro, na região dorsal de cada rato. A ferida recebeu o tratamento de acordo com o grupo ao qual pertencia: 1) aplicação das ADSCs no 15º dia (grupo tratado); 2) aplicação da solução cloreto de sódio 0,9% no 15º dia (grupo sham); 3) nenhuma intervenção até o momento da eutanásia (grupo controle). O grupo tratado com as ADSCs apresentou as maiores taxa...
Universidade Federal do Pampa, Jul 30, 2013
Comparative Clinical Pathology, 2018
Acanthocheilonema reconditum (A. reconditum) is a nematode with low pathogenicity that causes can... more Acanthocheilonema reconditum (A. reconditum) is a nematode with low pathogenicity that causes canine filariasis, without clinical signs. This filarid produces microfilariae that circulate in the blood of their natural hosts. Microfilariae of A. reconditum can be easily confused with microfilariae of Dirofilaria immitis (D. immitis) which causes a severe disease in dogs. The aim of this article is to describe a case of A. reconditum infection in a dog, diagnosed by fine needle aspiration cytology (FNAC) of two cutaneous nodules, blood smear examination, and further confirmed by molecular assay (PCR). This report alerts veterinarians to the presence of A. reconditum in the region of Santa Maria, Rio Grande do Sul, Brazil, and highlights the importance of performing the differentiation of microfilaria species. In addition, it demonstrates the importance of fine needle aspiration cytology as a diagnostic tool.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2005
To observe the short- and mid-term effects of percutaneous coronary intervention (PCI) in patient... more To observe the short- and mid-term effects of percutaneous coronary intervention (PCI) in patients with ST-segment elevation acute myocardial infarction (AMI) complicated by heart failure and/or cardiogenic shock . Altogether 90 patients with AMI were recruited, of whom 58 were treated by PCI, 20 by thrombolytic therapy, and the other received general treatment without reperfusion therapy. The length of hospital stay, major adverse cardiac events (MACE) and left ventricular ejection fraction (LVEF) were compared between PCI and thrombolysis groups. The relationship between the patency time of the infarct-related artery (IRA), thrombolysis in myocardial infarction (TIMI) grade after PCI and prognosis were analyzed in PCI group. The patency rate of IRA was significantly improved in patients receiving PCI therapy in comparison by those with thrombolytic therapy (98.3% vs 65.0%, P<0.01), and the LVEF was also higher in PCI group with lower mortality (6.9% vs 25.0%, P<0.05) during ...
Os perfis bioquimicos constituem dados importantes do paciente, alem de serem facilmente disponib... more Os perfis bioquimicos constituem dados importantes do paciente, alem de serem facilmente disponibilizados na rotina clinica da medicina veterinaria. A composicao do soro reflete a integridade celular e a funcao orgânica, possibilitando a deteccao de lesoes ou disfuncoes nos diferentes orgaos. O presente trabalho teve como objetivo determinar as concentracoes sericas de albumina, aspartato aminotransferase (AST), creatina quinase (CK), creatinina, fibrinogenio, gama glutamiltranferase (GGT) e proteinas plasmaticas totais de dezesseis equinos de tracao sem raca definida, naturalmente parasitados. Efetuaram-se colheitas de sangue dos animais em que, apos realizacao de exame quantitativo de fezes, foi constatado contagem superior a 300 ovos por grama de fezes. A mensuracao de proteina total e fibrinogenio foi realizada atraves de refratometria. As demais enzimas foram dosadas com auxilio de conjuntos de reagentes comerciais e leituras espectrofotometricas. Foram obtidas medias e desvios...
ABSTRACT. Lhamas C.L., Anjos B.L., Pfingstag K.G., Quevedo L.S. & Duarte C.A. [Schiff-Sherrington... more ABSTRACT. Lhamas C.L., Anjos B.L., Pfingstag K.G., Quevedo L.S. & Duarte C.A. [Schiff-Sherrington syndrome in a horse - Case report.] Sindrome de Schiff-Sherrington em equino - Relato de caso. Revista Brasileira de Medicina Veterinaria, 37(2):163-166, 2015. Curso de Pos-Graduacao em Ciencia Animal, Universidade Federal do Pampa, Campus Uruguaiana, BR 472, Km 585, Uruguaiana, RS 97500-970, Brasil. E-mail: claudiaduarte@unipampa.edu.br Schiff-Sherrington syndrome clinically corresponds to a manifestation of rigidity extensor or hypertonia of the forelimb and hypotonic paralysis of the hind limbs. It is a common condition in dogs, however, rarely described in large animals. It can be caused by trauma and spinal cord compression conditions. The aim of this study was to describe the case of a two-year-old male horse with Schiff-Sherrington syndrome. The animal was sent with signs of paresis and ataxia of the hind limbs, and during the necropsy, multiple fractures were observed in the 1st...
As fraturas dos corpos vertebrais toracolombares sao incomuns e podem ser causadas por acidentes ... more As fraturas dos corpos vertebrais toracolombares sao incomuns e podem ser causadas por acidentes em saltos, quedas em corridas e tetano. A apresentacao clinica e variavel, dependendo da localizacao, estruturas anatomicas envolvidas e gravidade da compressao medular. Este relato descreve um equino da raca Quarto-de-milha, dois anos de idade com fratura de 1a e 2a vertebras lombares. O animal apresentava paraparesia e ataxia dos membros pelvicos, estava alerta e se alimentava. Permanecia em decubito lateral e, quando estimulado a ficar em posicao quadrupedal, apoiava os membros toracicos, mas nao conseguia fazer o mesmo com os pelvicos, assumindo a posicao de cao sentado e, ao caminhar, arrastava as pincas dos membros pelvicos e os cruzava. Havia edema e sensibilidade dolorosa na regiao lombar dorsal. O animal mantinha o reflexo digital nos quatro membros durante prova de sensibilidade e reflexo anal, mas nao havia reflexo do paniculo, caudal as primeiras vertebras lombares. Nao apres...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2015
O objetivo do presente estudo foi avaliar a influência do parasitismo intestinal crônico sobre pa... more O objetivo do presente estudo foi avaliar a influência do parasitismo intestinal crônico sobre parâmetros hematológicos e de líquido peritoneal por meio da comparação dessas características em equinos naturalmente parasitados e após administração de anti-helmíntico. Utilizaram-se 21 cavalos de tração urbana, entre dois e 19 anos, sem raça definida e com resultado de exame parasitológico superior a 300 ovos por grama de fezes. Foi realizada avaliação física e coleta de fezes, de líquido peritoneal e de sangue em dois momentos do experimento (D0 e D15), sendo efetuado tratamento antiparasitário no D0. No fluido peritoneal foram avaliadas características físicas, bioquímicas, bem como contagem de células nucleadas (CTCN) e diferenciação celular. No sangue foram determinados valores eritrocitários, leucocitários, proteínas plasmáticas totais, glicose e fibrinogênio plasmáticos, além de fosfatase alcalina (FA) sérica. A análise dos parâmetros avaliados não demonstrou diferença significat...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2016
RESUMO A videocirurgia é atualmente uma das principais ferramentas operatórias, com vantagens que... more RESUMO A videocirurgia é atualmente uma das principais ferramentas operatórias, com vantagens que incluem menor estresse, incisões e dor pós-operatória quando comparada aos procedimentos abertos. Objetivou-se comparar o processo inflamatório e o estresse oxidativo resultantes das técnicas de ovário-histerectomia (OVH) convencional e videoassistida, com dois portais em cadelas, por meio de hemograma, avaliação de acetilcolinesterase, butirilcolinesterase, catalase e malondialdeído séricos, imediatamente antes da operação e duas, seis, 12, 24, 48 e 72 horas após a cirurgia. Observou-se menor estresse cirúrgico nas pacientes operadas pela técnica videoassistida, e sugere-se que a técnica convencional possa implicar peroxidação lipídica, mesmo com o uso de anti-inflamatório.
Drug and Chemical Toxicology
Brazilian Journal of Pharmaceutical Sciences
Verbena litoralis is a plant popularly known as "gervãozinho-do-campo" in Portuguese. It is tradi... more Verbena litoralis is a plant popularly known as "gervãozinho-do-campo" in Portuguese. It is traditionally used for stomach, liver and gallbladder problems, and as an anti-inflammatory and anthelmintic. The goal of this study was to determine the chemical composition of the crude extract obtained from the aerial parts of V. litoralis by Ultra High Performance Liquid chromatography coupled with Mass Spectrometry in Tandem (UHPLC-MS/MS); assess the anti-inflammatory activity of ethyl acetate fraction and of crude extract; and verify liver, kidney and pancreas damage. In this study, the chemical composition of the extract was identified via UHPLC/MS/MS, assessing the anti-inflammatory activity of the crude extract and the acetate fraction in an induction model of the granulomatous tissue, as well as given liver, kidney and pancreas damage markers. Chlorogenic acid, luteolin, caffeic acid, apigenin, p-coumaric acid, vanillic acid, ferulic acid and quercetin were quantified in the extract. After the seven-day treatment, the granuloma of the animals treated with the plant extract and fraction presented values very close to the positive control (nimesulide). The V. litoralis crude extract and ethyl acetate fraction show antiinflammatory activity similar to the nimesulide without evidence of liver, kidney and pancreas damage, which attributes the plant's pharmacological action to the flavonoids found.
Journal of Ethnopharmacology
Journal of ethnopharmacology, Jan 14, 2018
Verbena litoralis Kunth is a native species of South America, popularly known as gervãozinho-do-c... more Verbena litoralis Kunth is a native species of South America, popularly known as gervãozinho-do-campo ou erva-de-pai-caetano. It is used in gastrointestinal disorders, as detoxifying the organism, antifebrile properties and amidaglitis. To identify the chemical constituents of the hydroethanolic extract obtained from the aerial parts of V. litoralis and to evaluate the acute and sub-acute toxicity in male and female rats. The single dose (2000 mg/kg) of the extract was administered orally to male and female rats. In the subacute study the extract was given at doses of 100, 200 and 400mg/kg during 28 days orally. Biochemical, hematological and histological analyzes were performed, oxidative stress markers were tested and chemical constituents were identified through UHPLC-ESI-HRMS RESULTS: Six classes of metabolites were identified: iridoids glycosides, flavonoids, phenylpropanoids-derived, phenylethanoid-derived, cinnamic acid-derived and triterpenes. In the acute treatment, the ext...
Regulatory toxicology and pharmacology : RTP, Jan 17, 2018
Olea europaea L., popularly known as olive, is a plant widely used worldwide. Its leaves, fruit a... more Olea europaea L., popularly known as olive, is a plant widely used worldwide. Its leaves, fruit and oil are extensively consumed and present important pharmacological properties. However, studies regarding the toxicity of olive leaves are still limited in the literature. Therefore, the aim of the study was to investigate acute and subacute oral toxicities of the ethanolic extract of olive leaves (EEO) in Wistar rats through histopathology and biochemical and hematological parameters. Acute toxicity was assessed using a single dose of 2000 mg/kg of EEO administered by oral gavage to male and female rats. To assess subacute toxicity, EEO was administered during 28 days at different doses (100, 200 and 400 mg/kg) to male and female rats. At the end of the experiments, the liver and kidney were removed and examined microscopically, and blood was collected for hematological and biochemical parameters. A single dose of 2000 mg/kg did not induce mortality or any signs of toxicity among the...
Cryosurgery has been used to treat different diseases of systems and organs, although adverse eff... more Cryosurgery has been used to treat different diseases of systems and organs, although adverse effects have been reported such as delayed wound healing, large scars, esthetical deformation and functional impairment. Injuries caused naturally by the exposure to extreme cold weather conditions mostly result in gangrene. This study aims to evaluate the influence of adipose-derived stem cells (ADSCs) in the proliferation phase on cutaneous wound healing. Through the application of liquid nitrogen by the spraying technique, a 15 millimeter diameter lesion was produced in the dorsal region of each rat. The wound received treatment according to the group it belonged: 1) ADSCs application on the 15th day (treated group); 2) application of 0.9% sodium chloride solution on the 15th day (sham group); 3) no intervention until euthanasia (control group). The group treated with ADSCs showed the highest wound average contraction rate; this group got a significant statistical difference in relation ...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2020
RESUMO A criocirurgia tem sido utilizada no tratamento de diferentes enfermidades de sistemas e ó... more RESUMO A criocirurgia tem sido utilizada no tratamento de diferentes enfermidades de sistemas e órgãos. Contudo, são relatados efeitos adversos, como cicatrização lenta, cicatrizes extensas, disfunção estética e funcional. As lesões que ocorrem naturalmente pela exposição ao frio extremo, comumente, resultam em gangrena. O presente trabalho teve como objetivo avaliar a influência das células-tronco mesenquimais de origem adiposa (ADSCs) na fase de proliferação da cicatrização de feridas cutâneas. Por meio da aplicação do nitrogênio líquido pela técnica do spray aberto, realizou-se a indução de uma ferida, de aproximadamente 15mm de diâmetro, na região dorsal de cada rato. A ferida recebeu o tratamento de acordo com o grupo ao qual pertencia: 1) aplicação das ADSCs no 15º dia (grupo tratado); 2) aplicação da solução cloreto de sódio 0,9% no 15º dia (grupo sham); 3) nenhuma intervenção até o momento da eutanásia (grupo controle). O grupo tratado com as ADSCs apresentou as maiores taxa...
Universidade Federal do Pampa, Jul 30, 2013
Comparative Clinical Pathology, 2018
Acanthocheilonema reconditum (A. reconditum) is a nematode with low pathogenicity that causes can... more Acanthocheilonema reconditum (A. reconditum) is a nematode with low pathogenicity that causes canine filariasis, without clinical signs. This filarid produces microfilariae that circulate in the blood of their natural hosts. Microfilariae of A. reconditum can be easily confused with microfilariae of Dirofilaria immitis (D. immitis) which causes a severe disease in dogs. The aim of this article is to describe a case of A. reconditum infection in a dog, diagnosed by fine needle aspiration cytology (FNAC) of two cutaneous nodules, blood smear examination, and further confirmed by molecular assay (PCR). This report alerts veterinarians to the presence of A. reconditum in the region of Santa Maria, Rio Grande do Sul, Brazil, and highlights the importance of performing the differentiation of microfilaria species. In addition, it demonstrates the importance of fine needle aspiration cytology as a diagnostic tool.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2005
To observe the short- and mid-term effects of percutaneous coronary intervention (PCI) in patient... more To observe the short- and mid-term effects of percutaneous coronary intervention (PCI) in patients with ST-segment elevation acute myocardial infarction (AMI) complicated by heart failure and/or cardiogenic shock . Altogether 90 patients with AMI were recruited, of whom 58 were treated by PCI, 20 by thrombolytic therapy, and the other received general treatment without reperfusion therapy. The length of hospital stay, major adverse cardiac events (MACE) and left ventricular ejection fraction (LVEF) were compared between PCI and thrombolysis groups. The relationship between the patency time of the infarct-related artery (IRA), thrombolysis in myocardial infarction (TIMI) grade after PCI and prognosis were analyzed in PCI group. The patency rate of IRA was significantly improved in patients receiving PCI therapy in comparison by those with thrombolytic therapy (98.3% vs 65.0%, P<0.01), and the LVEF was also higher in PCI group with lower mortality (6.9% vs 25.0%, P<0.05) during ...
Os perfis bioquimicos constituem dados importantes do paciente, alem de serem facilmente disponib... more Os perfis bioquimicos constituem dados importantes do paciente, alem de serem facilmente disponibilizados na rotina clinica da medicina veterinaria. A composicao do soro reflete a integridade celular e a funcao orgânica, possibilitando a deteccao de lesoes ou disfuncoes nos diferentes orgaos. O presente trabalho teve como objetivo determinar as concentracoes sericas de albumina, aspartato aminotransferase (AST), creatina quinase (CK), creatinina, fibrinogenio, gama glutamiltranferase (GGT) e proteinas plasmaticas totais de dezesseis equinos de tracao sem raca definida, naturalmente parasitados. Efetuaram-se colheitas de sangue dos animais em que, apos realizacao de exame quantitativo de fezes, foi constatado contagem superior a 300 ovos por grama de fezes. A mensuracao de proteina total e fibrinogenio foi realizada atraves de refratometria. As demais enzimas foram dosadas com auxilio de conjuntos de reagentes comerciais e leituras espectrofotometricas. Foram obtidas medias e desvios...
ABSTRACT. Lhamas C.L., Anjos B.L., Pfingstag K.G., Quevedo L.S. & Duarte C.A. [Schiff-Sherrington... more ABSTRACT. Lhamas C.L., Anjos B.L., Pfingstag K.G., Quevedo L.S. & Duarte C.A. [Schiff-Sherrington syndrome in a horse - Case report.] Sindrome de Schiff-Sherrington em equino - Relato de caso. Revista Brasileira de Medicina Veterinaria, 37(2):163-166, 2015. Curso de Pos-Graduacao em Ciencia Animal, Universidade Federal do Pampa, Campus Uruguaiana, BR 472, Km 585, Uruguaiana, RS 97500-970, Brasil. E-mail: claudiaduarte@unipampa.edu.br Schiff-Sherrington syndrome clinically corresponds to a manifestation of rigidity extensor or hypertonia of the forelimb and hypotonic paralysis of the hind limbs. It is a common condition in dogs, however, rarely described in large animals. It can be caused by trauma and spinal cord compression conditions. The aim of this study was to describe the case of a two-year-old male horse with Schiff-Sherrington syndrome. The animal was sent with signs of paresis and ataxia of the hind limbs, and during the necropsy, multiple fractures were observed in the 1st...
As fraturas dos corpos vertebrais toracolombares sao incomuns e podem ser causadas por acidentes ... more As fraturas dos corpos vertebrais toracolombares sao incomuns e podem ser causadas por acidentes em saltos, quedas em corridas e tetano. A apresentacao clinica e variavel, dependendo da localizacao, estruturas anatomicas envolvidas e gravidade da compressao medular. Este relato descreve um equino da raca Quarto-de-milha, dois anos de idade com fratura de 1a e 2a vertebras lombares. O animal apresentava paraparesia e ataxia dos membros pelvicos, estava alerta e se alimentava. Permanecia em decubito lateral e, quando estimulado a ficar em posicao quadrupedal, apoiava os membros toracicos, mas nao conseguia fazer o mesmo com os pelvicos, assumindo a posicao de cao sentado e, ao caminhar, arrastava as pincas dos membros pelvicos e os cruzava. Havia edema e sensibilidade dolorosa na regiao lombar dorsal. O animal mantinha o reflexo digital nos quatro membros durante prova de sensibilidade e reflexo anal, mas nao havia reflexo do paniculo, caudal as primeiras vertebras lombares. Nao apres...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2015
O objetivo do presente estudo foi avaliar a influência do parasitismo intestinal crônico sobre pa... more O objetivo do presente estudo foi avaliar a influência do parasitismo intestinal crônico sobre parâmetros hematológicos e de líquido peritoneal por meio da comparação dessas características em equinos naturalmente parasitados e após administração de anti-helmíntico. Utilizaram-se 21 cavalos de tração urbana, entre dois e 19 anos, sem raça definida e com resultado de exame parasitológico superior a 300 ovos por grama de fezes. Foi realizada avaliação física e coleta de fezes, de líquido peritoneal e de sangue em dois momentos do experimento (D0 e D15), sendo efetuado tratamento antiparasitário no D0. No fluido peritoneal foram avaliadas características físicas, bioquímicas, bem como contagem de células nucleadas (CTCN) e diferenciação celular. No sangue foram determinados valores eritrocitários, leucocitários, proteínas plasmáticas totais, glicose e fibrinogênio plasmáticos, além de fosfatase alcalina (FA) sérica. A análise dos parâmetros avaliados não demonstrou diferença significat...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2016
RESUMO A videocirurgia é atualmente uma das principais ferramentas operatórias, com vantagens que... more RESUMO A videocirurgia é atualmente uma das principais ferramentas operatórias, com vantagens que incluem menor estresse, incisões e dor pós-operatória quando comparada aos procedimentos abertos. Objetivou-se comparar o processo inflamatório e o estresse oxidativo resultantes das técnicas de ovário-histerectomia (OVH) convencional e videoassistida, com dois portais em cadelas, por meio de hemograma, avaliação de acetilcolinesterase, butirilcolinesterase, catalase e malondialdeído séricos, imediatamente antes da operação e duas, seis, 12, 24, 48 e 72 horas após a cirurgia. Observou-se menor estresse cirúrgico nas pacientes operadas pela técnica videoassistida, e sugere-se que a técnica convencional possa implicar peroxidação lipídica, mesmo com o uso de anti-inflamatório.
Drug and Chemical Toxicology
Brazilian Journal of Pharmaceutical Sciences
Verbena litoralis is a plant popularly known as "gervãozinho-do-campo" in Portuguese. It is tradi... more Verbena litoralis is a plant popularly known as "gervãozinho-do-campo" in Portuguese. It is traditionally used for stomach, liver and gallbladder problems, and as an anti-inflammatory and anthelmintic. The goal of this study was to determine the chemical composition of the crude extract obtained from the aerial parts of V. litoralis by Ultra High Performance Liquid chromatography coupled with Mass Spectrometry in Tandem (UHPLC-MS/MS); assess the anti-inflammatory activity of ethyl acetate fraction and of crude extract; and verify liver, kidney and pancreas damage. In this study, the chemical composition of the extract was identified via UHPLC/MS/MS, assessing the anti-inflammatory activity of the crude extract and the acetate fraction in an induction model of the granulomatous tissue, as well as given liver, kidney and pancreas damage markers. Chlorogenic acid, luteolin, caffeic acid, apigenin, p-coumaric acid, vanillic acid, ferulic acid and quercetin were quantified in the extract. After the seven-day treatment, the granuloma of the animals treated with the plant extract and fraction presented values very close to the positive control (nimesulide). The V. litoralis crude extract and ethyl acetate fraction show antiinflammatory activity similar to the nimesulide without evidence of liver, kidney and pancreas damage, which attributes the plant's pharmacological action to the flavonoids found.
Journal of Ethnopharmacology
Journal of ethnopharmacology, Jan 14, 2018
Verbena litoralis Kunth is a native species of South America, popularly known as gervãozinho-do-c... more Verbena litoralis Kunth is a native species of South America, popularly known as gervãozinho-do-campo ou erva-de-pai-caetano. It is used in gastrointestinal disorders, as detoxifying the organism, antifebrile properties and amidaglitis. To identify the chemical constituents of the hydroethanolic extract obtained from the aerial parts of V. litoralis and to evaluate the acute and sub-acute toxicity in male and female rats. The single dose (2000 mg/kg) of the extract was administered orally to male and female rats. In the subacute study the extract was given at doses of 100, 200 and 400mg/kg during 28 days orally. Biochemical, hematological and histological analyzes were performed, oxidative stress markers were tested and chemical constituents were identified through UHPLC-ESI-HRMS RESULTS: Six classes of metabolites were identified: iridoids glycosides, flavonoids, phenylpropanoids-derived, phenylethanoid-derived, cinnamic acid-derived and triterpenes. In the acute treatment, the ext...
Regulatory toxicology and pharmacology : RTP, Jan 17, 2018
Olea europaea L., popularly known as olive, is a plant widely used worldwide. Its leaves, fruit a... more Olea europaea L., popularly known as olive, is a plant widely used worldwide. Its leaves, fruit and oil are extensively consumed and present important pharmacological properties. However, studies regarding the toxicity of olive leaves are still limited in the literature. Therefore, the aim of the study was to investigate acute and subacute oral toxicities of the ethanolic extract of olive leaves (EEO) in Wistar rats through histopathology and biochemical and hematological parameters. Acute toxicity was assessed using a single dose of 2000 mg/kg of EEO administered by oral gavage to male and female rats. To assess subacute toxicity, EEO was administered during 28 days at different doses (100, 200 and 400 mg/kg) to male and female rats. At the end of the experiments, the liver and kidney were removed and examined microscopically, and blood was collected for hematological and biochemical parameters. A single dose of 2000 mg/kg did not induce mortality or any signs of toxicity among the...