Claude Carelli - Academia.edu (original) (raw)
Papers by Claude Carelli
Proceedings of the National Academy of Sciences of the United States of America, Aug 15, 1993
International Journal of Peptide and Protein Research, 2009
Two glycopeptides associating the aminoacid sequence of LH‐RH with MDP were prepared, using a Lys... more Two glycopeptides associating the aminoacid sequence of LH‐RH with MDP were prepared, using a Lys residue as a linker. These conjugates, Nα MDP Ne (LH‐RH)‐Lys and Nα MDP Ne (LH‐RH)‐Lys‐NH2 obtained by condensation of fragments were synthesized by liquid as well as solid phase methods. Both compounds were able to induce anti LH‐RH antibodies and immunological castration. They retained the immuno‐adjuvant activity of MDP. Such antigen‐adjuvant constructs, devoid of carrier and obtained by chemically defined and reproducible synthetic methods could offer suitable tools for structure‐activity relationship studies aiming at defining synthetic vaccines.
Osteoarthritis and Cartilage, 2019
Circulation, 1990
Spontaneously hypertensive Okamoto-strain rats (SHR) and normotensive Wistar-Kyoto (WKY) rats wer... more Spontaneously hypertensive Okamoto-strain rats (SHR) and normotensive Wistar-Kyoto (WKY) rats were actively immunized with mouse renin to investigate the effect on blood pressure of blocking the renin-angiotensinogen reaction. Ten male SHR and 10 male WKY rats were immunized with purified mouse submandibular gland renin. Control rats were immunized with bovine serum albumin. Antirenin antibodies were produced by both SHR and WKY rats, but renin-immunized SHR had higher titers of circulating renin antibodies after three injections. The increase in renin antibody in renin-immunized SHR was associated with a significant drop in blood pressure (tail-cuff method) that became similar to that of the WKY control rats after four injections. The blockade by antirenin immunoglobulins of the renin-angiotensinogen reaction also decreased the blood pressure of normotensive rats. Perfusion of renin-immunized rats with mouse submandibular renin (10 micrograms) in vivo caused no increase in blood pr...
International Journal of Immunopharmacology, 1985
Archives des maladies du coeur et des vaisseaux, 1988
To block the renin-angiotensin system by antibodies directed against renin or angiotensins is an ... more To block the renin-angiotensin system by antibodies directed against renin or angiotensins is an old and recent goal. This goal can be attained by passive transfer of antibodies or by active immunization against the different molecules of the system. Only passive transfer of polyclonal antibodies directed against the native substrate (angiotensinogen) has been performed in rats. This acute blockade of angiotensinogen substrate availability decrease blood pressure about 30 mmHg in salt depleted rats. Passive transfer of anti-converting enzyme immunoglobulins has been already performed in rabbit and rat. It induced an immunoallergic reaction in the pulmonary capillary bed. Immunization against angiotensin II has been a powerful tool in the exploration of the role of the renin angiotensin system in hypertension. Passive and active immunization have been performed in different species: rabbit, rat. The majority of the results concerning the decrease in blood pressure was negative. Howev...
Biomedicine & Pharmacotherapy, 1992
We have designed two software systems allowing the study of proteins through a comparison to thos... more We have designed two software systems allowing the study of proteins through a comparison to those stored in data banks. The first one, "Automat", locates in a systematic manner all identities shared by a given protein and the proteins in a data bank. The second, "Critic" enables the selection of specific segments in a given molecule by comparing them with those gathered in a data bank. These sites were termed "critical" since they mostly correspond to functional sites (active sites) of the well-known proteins which were studied with the aid of this program (somatostatin, insulin, IL2, etc). Automat allowed us to reveal homologies between HIV-1 and the CD4, which have remained unsolved until now. These similitudes proved to be critical sites (according to Critic). The putative involvement of these sites in the physiopathological processes as induced by HIV-1 are worth considering since the results of our experiments are consistent with this assumption.
Biomedicine & Pharmacotherapy, 1993
We investigate the validity of the equivalence principle along paths in gravitational theories ba... more We investigate the validity of the equivalence principle along paths in gravitational theories based on derivations of the tensor algebra over a differentiable manifold. We prove the existence of local bases, called normal, in which the components of the derivations vanish along arbitrary paths. All such bases are explicitly described. The holonomicity of the normal bases is considered. The results obtained are applied to the important case of linear connections and their relationship with the equivalence principle is described. In particular, any gravitational theory based on tensor derivations which obeys the equivalence principle along all paths, must be based on a linear connection.
Biopolymers, 2014
This article has been accepted for publication and undergone full peer review but has not been th... more This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as an 'Accepted Article',
Biomedicine & Pharmacotherapy, 1992
In the first AIDS vaccine trial, immunizing preparations were based on HIV-1 Env protein (gp160).... more In the first AIDS vaccine trial, immunizing preparations were based on HIV-1 Env protein (gp160). Immunogenic properties of gp160 which trigger both a humoral and cellular immune response have since justified its use in various vaccine programs, both past and present. Many reports however have underlined deleterious effects on the immune system--anti-HIV-1 enhanced antibodies, anti-CD4 autoantibodies, and inhibition of T cell activation by HIV-1--particularly associated with the Env protein. The present study shows that gp160 presented in a biologically inactivated but immunogenic form, as used in our trial, could avoid these complications. Bio-hazards associated with gp160 which indeed could be removed by appropriate treatment of the native protein, should be taken into consideration in AIDS vaccine programs.
The invention concerns growth hormone agonist peptides and their uses as medicines, foods, and ag... more The invention concerns growth hormone agonist peptides and their uses as medicines, foods, and agents for stimulating in vitro cell proliferation.
PLOS ONE, Apr 14, 2020
ObjectiveREG-O3 is a 24-aminoacid chimeric peptide combining a sequence derived from growth hormo... more ObjectiveREG-O3 is a 24-aminoacid chimeric peptide combining a sequence derived from growth hormone (GH) and an analog of somatostatin (SST), molecules displaying cartilage repair and anti-inflammatory properties, respectively. This study aimed to investigate the disease-modifying osteoarthritis drug (DMOAD) potential of REG-O3 by analyzing its effect on pain, joint function and structure, upon injection into osteoarthritic rat knee joint.DesignOsteoarthritis was induced in the right knee of mature male Lewis rats (n = 12/group) by surgical transection of the anterior cruciate ligament (ACLT) combined with partial medial meniscectomy (pMMx). Treatments were administered intra-articularly from fourteen days after surgery through three consecutive injections one week apart. The effect of REG-O3, solubilized in a liposomal solution and injected at either 5, 25 or 50 μg/50 μL, was compared to liposomal (LIP), dexamethasone and hyaluronic acid (HA) solutions. The study endpoints were the pain/function measured once a week throughout the entire study, and the joint structure evaluated eight weeks after surgery using OARSI score.ResultsACLT/pMMx surgery induced a significant modification of weight bearing in all groups. When compared to liposomal solution, REG-O3 was able to significantly improve weight bearing as efficiently as dexamethasone and HA. REG-O3 (25 μg) was also able to significantly decrease OARSI histological global score as well as degeneration of both cartilage and matrix while the other treatments did not.ConclusionThis study provides evidence of a remarkable protecting effect of REG-O3 on pain/knee joint function and cartilage/matrix degradation in ACLT/pMMx model of rat osteoarthritis. REG-O3 thus displays an interesting profile as a DMOAD.
Methods in Protein Sequence Analysis, 1989
Renin (EC 3.4.23.15), an aspartyl protease, is a rate limiting factor on angiotensin II productio... more Renin (EC 3.4.23.15), an aspartyl protease, is a rate limiting factor on angiotensin II production which is a potent vasoconstrictor peptide implicated in the regulation of blood pressure and electrolyte metabolism. So renin is an important target in the design of antihypertensive drugs. Different approaches in the inhibition of the renin-angiotensinogen system have been adopted, such as the use of substrate analogues (Szelke et al., 1982), statine-containing peptides (Boger et al., 1983, 1985; Guegan et al., 1986), peptides derived from the renin profragment sequence (Cumin et al., 1985) and specific monoclonal renin antibodies (Galen et al., 1984). Recently, specific blockade of the renin-angiotensinogen system has been obtained by active immunization against renin in normotensive marmoset (Michel et al., 1987) but this active immunization induces an autoimmune desease, so it was worthwhile to conduct experiments with synthetic peptides related to renin sequence and to try to determinate the major renin epitopes.
Proceedings of the National Academy of Sciences of the United States of America, Aug 15, 1993
International Journal of Peptide and Protein Research, 2009
Two glycopeptides associating the aminoacid sequence of LH‐RH with MDP were prepared, using a Lys... more Two glycopeptides associating the aminoacid sequence of LH‐RH with MDP were prepared, using a Lys residue as a linker. These conjugates, Nα MDP Ne (LH‐RH)‐Lys and Nα MDP Ne (LH‐RH)‐Lys‐NH2 obtained by condensation of fragments were synthesized by liquid as well as solid phase methods. Both compounds were able to induce anti LH‐RH antibodies and immunological castration. They retained the immuno‐adjuvant activity of MDP. Such antigen‐adjuvant constructs, devoid of carrier and obtained by chemically defined and reproducible synthetic methods could offer suitable tools for structure‐activity relationship studies aiming at defining synthetic vaccines.
Osteoarthritis and Cartilage, 2019
Circulation, 1990
Spontaneously hypertensive Okamoto-strain rats (SHR) and normotensive Wistar-Kyoto (WKY) rats wer... more Spontaneously hypertensive Okamoto-strain rats (SHR) and normotensive Wistar-Kyoto (WKY) rats were actively immunized with mouse renin to investigate the effect on blood pressure of blocking the renin-angiotensinogen reaction. Ten male SHR and 10 male WKY rats were immunized with purified mouse submandibular gland renin. Control rats were immunized with bovine serum albumin. Antirenin antibodies were produced by both SHR and WKY rats, but renin-immunized SHR had higher titers of circulating renin antibodies after three injections. The increase in renin antibody in renin-immunized SHR was associated with a significant drop in blood pressure (tail-cuff method) that became similar to that of the WKY control rats after four injections. The blockade by antirenin immunoglobulins of the renin-angiotensinogen reaction also decreased the blood pressure of normotensive rats. Perfusion of renin-immunized rats with mouse submandibular renin (10 micrograms) in vivo caused no increase in blood pr...
International Journal of Immunopharmacology, 1985
Archives des maladies du coeur et des vaisseaux, 1988
To block the renin-angiotensin system by antibodies directed against renin or angiotensins is an ... more To block the renin-angiotensin system by antibodies directed against renin or angiotensins is an old and recent goal. This goal can be attained by passive transfer of antibodies or by active immunization against the different molecules of the system. Only passive transfer of polyclonal antibodies directed against the native substrate (angiotensinogen) has been performed in rats. This acute blockade of angiotensinogen substrate availability decrease blood pressure about 30 mmHg in salt depleted rats. Passive transfer of anti-converting enzyme immunoglobulins has been already performed in rabbit and rat. It induced an immunoallergic reaction in the pulmonary capillary bed. Immunization against angiotensin II has been a powerful tool in the exploration of the role of the renin angiotensin system in hypertension. Passive and active immunization have been performed in different species: rabbit, rat. The majority of the results concerning the decrease in blood pressure was negative. Howev...
Biomedicine & Pharmacotherapy, 1992
We have designed two software systems allowing the study of proteins through a comparison to thos... more We have designed two software systems allowing the study of proteins through a comparison to those stored in data banks. The first one, "Automat", locates in a systematic manner all identities shared by a given protein and the proteins in a data bank. The second, "Critic" enables the selection of specific segments in a given molecule by comparing them with those gathered in a data bank. These sites were termed "critical" since they mostly correspond to functional sites (active sites) of the well-known proteins which were studied with the aid of this program (somatostatin, insulin, IL2, etc). Automat allowed us to reveal homologies between HIV-1 and the CD4, which have remained unsolved until now. These similitudes proved to be critical sites (according to Critic). The putative involvement of these sites in the physiopathological processes as induced by HIV-1 are worth considering since the results of our experiments are consistent with this assumption.
Biomedicine & Pharmacotherapy, 1993
We investigate the validity of the equivalence principle along paths in gravitational theories ba... more We investigate the validity of the equivalence principle along paths in gravitational theories based on derivations of the tensor algebra over a differentiable manifold. We prove the existence of local bases, called normal, in which the components of the derivations vanish along arbitrary paths. All such bases are explicitly described. The holonomicity of the normal bases is considered. The results obtained are applied to the important case of linear connections and their relationship with the equivalence principle is described. In particular, any gravitational theory based on tensor derivations which obeys the equivalence principle along all paths, must be based on a linear connection.
Biopolymers, 2014
This article has been accepted for publication and undergone full peer review but has not been th... more This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as an 'Accepted Article',
Biomedicine & Pharmacotherapy, 1992
In the first AIDS vaccine trial, immunizing preparations were based on HIV-1 Env protein (gp160).... more In the first AIDS vaccine trial, immunizing preparations were based on HIV-1 Env protein (gp160). Immunogenic properties of gp160 which trigger both a humoral and cellular immune response have since justified its use in various vaccine programs, both past and present. Many reports however have underlined deleterious effects on the immune system--anti-HIV-1 enhanced antibodies, anti-CD4 autoantibodies, and inhibition of T cell activation by HIV-1--particularly associated with the Env protein. The present study shows that gp160 presented in a biologically inactivated but immunogenic form, as used in our trial, could avoid these complications. Bio-hazards associated with gp160 which indeed could be removed by appropriate treatment of the native protein, should be taken into consideration in AIDS vaccine programs.
The invention concerns growth hormone agonist peptides and their uses as medicines, foods, and ag... more The invention concerns growth hormone agonist peptides and their uses as medicines, foods, and agents for stimulating in vitro cell proliferation.
PLOS ONE, Apr 14, 2020
ObjectiveREG-O3 is a 24-aminoacid chimeric peptide combining a sequence derived from growth hormo... more ObjectiveREG-O3 is a 24-aminoacid chimeric peptide combining a sequence derived from growth hormone (GH) and an analog of somatostatin (SST), molecules displaying cartilage repair and anti-inflammatory properties, respectively. This study aimed to investigate the disease-modifying osteoarthritis drug (DMOAD) potential of REG-O3 by analyzing its effect on pain, joint function and structure, upon injection into osteoarthritic rat knee joint.DesignOsteoarthritis was induced in the right knee of mature male Lewis rats (n = 12/group) by surgical transection of the anterior cruciate ligament (ACLT) combined with partial medial meniscectomy (pMMx). Treatments were administered intra-articularly from fourteen days after surgery through three consecutive injections one week apart. The effect of REG-O3, solubilized in a liposomal solution and injected at either 5, 25 or 50 μg/50 μL, was compared to liposomal (LIP), dexamethasone and hyaluronic acid (HA) solutions. The study endpoints were the pain/function measured once a week throughout the entire study, and the joint structure evaluated eight weeks after surgery using OARSI score.ResultsACLT/pMMx surgery induced a significant modification of weight bearing in all groups. When compared to liposomal solution, REG-O3 was able to significantly improve weight bearing as efficiently as dexamethasone and HA. REG-O3 (25 μg) was also able to significantly decrease OARSI histological global score as well as degeneration of both cartilage and matrix while the other treatments did not.ConclusionThis study provides evidence of a remarkable protecting effect of REG-O3 on pain/knee joint function and cartilage/matrix degradation in ACLT/pMMx model of rat osteoarthritis. REG-O3 thus displays an interesting profile as a DMOAD.
Methods in Protein Sequence Analysis, 1989
Renin (EC 3.4.23.15), an aspartyl protease, is a rate limiting factor on angiotensin II productio... more Renin (EC 3.4.23.15), an aspartyl protease, is a rate limiting factor on angiotensin II production which is a potent vasoconstrictor peptide implicated in the regulation of blood pressure and electrolyte metabolism. So renin is an important target in the design of antihypertensive drugs. Different approaches in the inhibition of the renin-angiotensinogen system have been adopted, such as the use of substrate analogues (Szelke et al., 1982), statine-containing peptides (Boger et al., 1983, 1985; Guegan et al., 1986), peptides derived from the renin profragment sequence (Cumin et al., 1985) and specific monoclonal renin antibodies (Galen et al., 1984). Recently, specific blockade of the renin-angiotensinogen system has been obtained by active immunization against renin in normotensive marmoset (Michel et al., 1987) but this active immunization induces an autoimmune desease, so it was worthwhile to conduct experiments with synthetic peptides related to renin sequence and to try to determinate the major renin epitopes.